A composite for ocular disease repair and a method of preparing the composite by aging extraction

Abstract

The application discloses a compound for eye disease repair and a preparation method of the compound by aging extraction, which is synthesized by extracting effective components of Senecio scandens Buch, astaxanthin, Artemisia capillaris Thunb, Gardenia, Ceti, Cassia seed, and has good therapeutic effect on eye diseases such as myopia, pseudomyopia, cataract, glaucoma, chloasma and the like. By taking the extract, intraocular pressure, optic nerve and intraocular pressure tube are gradually improved, so that the eye axis refraction is basically recovered, and the purpose of basic cure is achieved. The extract is obtained by supercritical extraction of pure plants and traditional Chinese medicines, and the compound Tianmocai compound of Senecio scandens Buch is obtained by using three reaction kettles to induce aging technology, the compound is safe and has no side effect, and is an innovative modern new Chinese medicine technical product.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical technology, and in particular to a complex for the repair of eye diseases and a method for its preparation by aging and extraction. Background Technology

[0002] There is a huge demand for ophthalmological medical services in my country. Due to the widespread use of electronic products, the incidence of myopia at a young age is alarmingly high. Simultaneously, with the arrival of an aging society, age-related eye diseases (such as cataracts, presbyopia, and retinal diseases) are increasing. Whether it's the myopia problem in children and adolescents or the eye health needs of the growing elderly, there is a strong need for professional ophthalmological medical institutions to provide better and more comprehensive eye health services to the general public. The incidence of myopia in my country is: 22.78% among primary school students; 55.22% among middle school students; and 70.34% among high school students. The prevalence of cataracts in people over 80 years old is almost 100%. The large population with sub-optimal eye health urgently needs professional and systematic medical services.

[0003] Currently, drug treatment accounts for approximately 25% of ophthalmic disease treatments, while surgery accounts for approximately 75%. Ophthalmic medications (mainly Western medicine) primarily focus on glaucoma, retinal diseases, and artificial tears. Ophthalmic surgery has a wider range of applications, used for various major ophthalmic diseases. However, surgery is not recommended for children aged 5-18 with myopia. Patients with other eye diseases face significant surgical risks, with a high risk of relapse, instability, and regression. Cataract patients require surgery to restore normal vision, and the risks of surgery vary depending on factors such as postoperative risks and immune system weakening in elderly patients. Summary of the Invention

[0004] The purpose of this invention is to provide a compound for the repair of eye diseases. This compound, taken orally, can restore intraocular pressure, optic nerve, and tonometer function without surgery or harm to the body, thereby restoring the axial length and refractive power of the eye and achieving a basic cure.

[0005] To achieve the above objectives, the present invention is implemented according to the following technical solution:

[0006] This invention discloses a complex for the repair of eye diseases, which is synthesized from extracts of Senecio scandens, astaxanthin, Artemisia capillaris, Gardenia jasminoides, Lygodium japonicum, and Cassia tora.

[0007] Preferably, by weight, it contains 5 parts of the Senecio scandens extract, 3 parts of the astaxanthin, 2 parts of Artemisia capillaris, 2 parts of Gardenia jasminoides, 2 parts of Lygodium japonicum, and 2 parts of Cassia tora.

[0008] The preparation method of the complex for eye disease repair of the present invention includes the following steps:

[0009] S1: Select fresh, high-quality Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora for the first aging process;

[0010] S2: The once aged Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora seeds were subjected to supercritical extraction to obtain high-purity extracts.

[0011] S3: The supercritical extraction extracts of Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora were placed into a reaction vessel for a second microbial aging treatment.

[0012] S4: The extracts of Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora after the second aging were synthesized into a complex according to the principles of traditional Chinese medicine.

[0013] S5: The complex is placed in a reactor for a third organic aging process to obtain the Xinsenmai compound Tianmu complex.

[0014] The beneficial effects of this invention are:

[0015] This invention relates to a compound for the repair of eye diseases. Compared with existing technologies, this invention has good therapeutic effects on eye diseases such as myopia, pseudomyopia, cataracts, glaucoma, and melasma. By taking the extract orally, it restores intraocular pressure, optic nerve, and tonometer function, essentially restoring the axial length and refractive power of the eye, achieving a basic cure. This invention uses pure plant and traditional Chinese medicine extracts obtained through supercritical fluid extraction, and employs a three-stage reaction vessel induced aging technology to obtain the Xinsenmai Compound Tianmu Compound. This compound is safe, has no side effects, and is an innovative modern traditional Chinese medicine technology product. Detailed Implementation

[0016] The present invention will be further described below with reference to specific embodiments. The illustrative embodiments and descriptions herein are used to explain the present invention, but are not intended to limit the present invention.

[0017] This invention discloses a complex for the repair of eye diseases, which is synthesized from extracts of Senecio scandens, astaxanthin, Artemisia capillaris, Gardenia jasminoides, Lygodium japonicum, and Cassia tora.

[0018] Preferably, by weight, it contains 5 parts of the Senecio scandens extract, 3 parts of the astaxanthin, 2 parts of Artemisia capillaris, 2 parts of Gardenia jasminoides, 2 parts of Lygodium japonicum, and 2 parts of Cassia tora.

[0019] The Senecio scandens extract is extracted from Senecio scandens, the astaxanthin extract is extracted from Haematococcus pluvialis, the Artemisia capillaris extract is extracted from Artemisia capillaris, the Gardenia jasminoides extract is extracted from Gardenia jasminoides, the Lygodium japonicum extract is extracted from the sporangia of the leaves of Lygodium japonicum, and the Cassia tora extract is extracted from Cassia tora. All of these extraction processes employ supercritical fluid extraction technology.

[0020] The *Senecio scandens* and *Haematococcus pluvialis* were aged in a reaction vessel before being extracted from *Artemisia capillaris*, *Gardenia jasminoides*, *Gynostemma pentaphyllum*, and *Cassia tora*.

[0021] This compound can treat: myopia, pseudomyopia, cataracts, glaucoma, melasma, and other diseases;

[0022] Senecio scandens;

[0023] English name: Climbing Groundsel Herb;

[0024] Chemical name: Hydroquinone;

[0025] Chemical formula: C6H6O2

[0026] Senecio scandens has the effects of clearing heat and detoxifying, clearing the liver and improving eyesight, and promoting diuresis and relieving itching. It is mainly used to treat carbuncles, boils, sores, red and swollen eyes, diarrhea and dysentery.

[0027] Senecio scandens has broad-spectrum antibiotic activity, exhibiting strong inhibitory effects against Staphylococcus aureus, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Shigella, Shigella boydii, and Shigella sonnei. In vitro, Senecio scandens (2 mg / ml) showed bactericidal activity against Staphylococcus aureus and Shigella typhi. At higher concentrations, it also inhibited pathogenic Escherichia coli and Pseudomonas aeruginosa. It also showed varying degrees of inhibitory effects against Proteus, Bacillus cereus, Anthrax, and Micrococcus occulta.

[0028] Treatment for eye diseases primarily involves anti-infection, mainly for treating conjunctivitis and blepharitis caused by wind and heat. In ancient times, eye drops were used to treat inflammatory diseases such as blepharitis and wind-related eye diseases. In modern times, it is mainly used for various ophthalmic infections and also to treat eye damage caused by autoimmune diseases.

[0029] Astaxanthin:

[0030] Foreign name: Astaxanthin[7]

[0031] Production methods: natural extraction, chemical synthesis, etc.

[0032] Extraction sources: Haematococcus pluvialis, Chlorococcus pluvialis, etc.;

[0033] Main functions: strong antioxidant, enhances immunity, enhances cell activity, anti-inflammatory, relieves fatigue, etc.

[0034] Antioxidant: Astaxanthin has a strong antioxidant capacity. After the human body absorbs this substance, it can synthesize a variety of intermediate products such as carotene, lutein, and canthaxanthin. It can effectively inhibit the oxidation reaction in the human body, and can also remove free radicals and lipid peroxides in the human body, helping to maintain cardiovascular health and delay cell aging.

[0035] Immunomodulation: Astaxanthin exerts its immunomodulatory effect by enhancing cellular and humoral immunity. In terms of enhancing cellular immunity, it protects immune cells by inhibiting the cytotoxic effects of singlet oxygen, while enhancing the specific immune responses of Th1 and Th2 cells, increasing the number of immune cells, and enhancing the activity of B cells. It can also improve serum complement activity, enhance the ability of interleukin-2 and interferon-γ, and enhance humoral immunity, thereby improving the body's immunity from both cellular and humoral perspectives.

[0036] Enhanced cell activity: Astaxanthin can effectively regulate the metabolism of articular chondrocytes, enhance cell activity, delay apoptosis, and protect joints and cartilage.

[0037] Anti-inflammatory: The inflammatory response is a normal physiological process by which the body fights against adverse external stimuli. Its main components include inflammatory cells and inflammatory mediators. Oxygen free radicals, as pro-inflammatory mediators, promote the inflammatory response. Astaxanthin alleviates skin inflammation by inhibiting the secretion and expression of pro-inflammatory factors such as nitric oxide, tumor necrosis factor α, prostaglandin E2 (PGE2), IL-1β, and IL-6. Simultaneously, through its antioxidant function, it can block the positive feedback loop between oxidative stress and pro-inflammatory factors.

[0038] Relieving fatigue: Astaxanthin can relieve physical fatigue, alleviate post-exercise fatigue, enhance the function of the oxidative stress system, reduce the accumulation of blood lactic acid after exercise, and thus improve athletic performance to a certain extent.

[0039] The effects of astaxanthin on eye diseases:

[0040] Astaxanthin, with its powerful antioxidant and free radical scavenging effects, improves retinal blood supply and has a very good therapeutic effect on age-related macular degeneration. Cataracts, or clouding of the lens of the eye, are mainly caused by the gradual oxidation of the lens cell membrane by free radicals. Astaxanthin's strong antioxidant and free radical scavenging effects can assist in the treatment and prevention of cataracts. Retinitis pigmentosa (night blindness) is a chronic, progressive, hereditary, nutritional retinal pigment disease caused by degeneration of retinal pigment cells. Astaxanthin can increase retinal pigment density and improve the treatment rate. Diabetic retinopathy is caused by long-term hyperglycemia, leading to obstruction of retinal capillaries, slowed blood flow, tissue hypoxia, and capillary fragility. This results in aneurysms on the posterior retina, punctate or patchy hemorrhages, and cotton-wool exudates, causing visual loss. Astaxanthin can significantly improve the resistance of blood vessels, restore the imbalance of osmotic pressure inside and outside blood vessels, reduce vascular permeability, inhibit the leakage of substances from blood vessels, ensure the integrity of blood vessels, and allow the eyes to receive sufficient blood supply. Therefore, it has good effects on improving myopia refractive error and axial length, relieving eye fatigue, and also helps to restore the tissue morphology of the sclera in myopic eyes.

[0041] Astaxanthin is a ketocarotenoid with the chemical name 3,3′-dihydroxy-4,4′-diketo-β,β′-carotene. It is a red solid powder that is fat-soluble, insoluble in water, but soluble in organic solvents.

[0042] Artemisia capillaris:

[0043] English name: Capillary Wormwood Herb.

[0044] Extraction source: Extracted from the dried aerial parts of Artemisia scoparia Waldst. et Kit. or Artemisia capillaris Thunb. (Asteraceae family) after aging.

[0045] Water solubility: Easily soluble in water.

[0046] Efficacy and effects: Clears damp-heat and relieves jaundice. It belongs to the category of diuretic and dampness-reducing drugs.

[0047] It has choleretic, antipyretic, hepatoprotective, lipid-lowering, blood pressure-lowering, asthma-relieving, antibacterial, antiviral, diuretic, anticancer, analgesic, anticaries, and anti-inflammatory effects, and has an inhibitory effect on hepatitis virus.

[0048] Gardenia:

[0049] English name: Cape Jasmine Fruit.

[0050] Extraction source: Extracted from the aged fruit of Gardenia jasminoides, a dicotyledonous plant belonging to the Rubiaceae family.

[0051] Main effects: Clears heat and relieves irritability, clears heat and promotes diuresis, cools blood and detoxifies, clears liver and improves eyesight, reduces swelling and relieves pain.

[0052] It has the effects of cooling, antipyretics, anti-inflammation, analgesia, sedation, lowering blood pressure, anticonvulsant, choleretic and hepatoprotective effects, and promoting pancreatic secretion; it also has inhibitory effects on Staphylococcus aureus and skin fungi.

[0053] When combined with various traditional Chinese medicines, it can treat pterygium, canthal conjunctivitis and late-stage keratitis; optic nerve atrophy, glaucoma, retinal vein occlusion; glaucoma, retinal vein thrombosis, vitreous hemorrhage caused by retinal periphlebitis; bacterial keratitis, etc.

[0054] Haijinsha:

[0055] English name: Lygodium japonicum (Thunb.) Sw.

[0056] Extraction source: Dried mature spores of Lygodium japonicum (Thunb.) Sw., a plant of the Lygodian family. The vines and leaves are harvested in autumn before the spores fall off, dried, and the spores are extracted by rubbing or crushing. The vines and leaves are then removed. Mature spores are aged before extraction.

[0057] Main effects: Clears heat and dampness, promotes urination and relieves pain, promotes bile secretion, antiviral and antibacterial, invigorates blood circulation and unblocks collaterals.

[0058] It is used for urinary tract infections, including those caused by heat, sand, stones, blood, and chyluria, as well as urethral pain. It is also used to treat urinary tract infections, stones, edema, sore throat, and shingles.

[0059] Cassia seeds:

[0060] English name: Semen Cassiae.

[0061] Extraction source: Mature seeds of Cassia obtusifolia L. (a legume), extracted after aging.

[0062] Main effects: Clears the liver and improves eyesight, promotes urination and bowel movements, lowers lipids and blood pressure.

[0063] It is used for symptoms such as red, swollen, and painful eyes, photophobia with excessive tearing, glaucoma, and cataracts. It is suitable for treating eye diseases caused by liver heat and wind-heat in the liver meridian. It can also treat headaches, dizziness, constipation, and other diseases.

[0064] Supercritical extraction:

[0065] Supercritical fluids are substances in a state that is neither gaseous nor liquid, existing only when their temperature and pressure exceed a critical point. Supercritical fluids have a high density, similar to liquids, while their viscosity is closer to that of gases. Therefore, supercritical fluids are ideal extractants.

[0066] The principle of supercritical CO2 fluid extraction (SFE) separation process is based on the relationship between the solubility of a supercritical fluid and its density, specifically utilizing the influence of pressure and temperature on the solubility of supercritical fluids. In the supercritical state, the supercritical fluid is brought into contact with the substances to be separated, selectively extracting components based on polarity, boiling point, and molecular weight. While the extracts obtained at different pressure ranges will not be singular, optimally proportioned mixtures can be obtained by controlling the conditions. Then, by reducing pressure and increasing temperature, the supercritical fluid is converted into a normal gas, causing the extracted substances to precipitate completely or substantially, thus achieving separation and purification. Therefore, the supercritical CO2 fluid extraction process is a combination of extraction and separation processes.

[0067] The critical pressure of CO2 is 7.3 MPa, and its critical temperature is 31.1 °C. When the pressure and temperature of CO2 exceed 7.3 MPa and 31.1 °C respectively, this state of CO2 is called supercritical CO2. In the supercritical state, CO2 fluid is a compressible, high-density fluid with properties between liquid and gas, exhibiting characteristics of both gas and liquid phases: its density is close to that of a liquid, its viscosity is close to that of a gas, and its self-diffusion coefficient is 100 times that of a liquid. Therefore, it possesses both a high diffusion coefficient and low viscosity comparable to that of a gas, and a density similar to that of a liquid, resulting in a strong dissolving ability for certain substances. Supercritical CO2 can be said to have special permeability and dissolving power for certain materials. Near the supercritical point, the solubility of CO2 increases rapidly with slight changes in temperature or pressure. Supercritical CO2 extraction achieves extraction and separation by controlling changes in pressure and temperature to increase the solubility of CO2 in substances.

[0068] Applications of supercritical fluid extraction technology in the pharmaceutical industry:

[0069] Used for the extraction of effective components from traditional Chinese medicine, the purification of heat-sensitive biological products, and the separation of lipid mixtures;

[0070] In the food industry, it is used for hop extraction and pigment extraction; in the fragrance industry, for the refining of natural and synthetic flavors; and in the chemical industry, for the separation of mixtures. Specific applications can be categorized as follows:

[0071] 1. Extraction of bioactive molecules from medicinal plants, extraction and separation of alkaloids;

[0072] 2. Lipids from different microorganisms, either for lipid recovery or for removing lipids from glycosides and proteins;

[0073] 3. Extract anti-cancer substances from various plants, especially paclitaxel obtained from the bark and leaves of the yew tree for cancer prevention and treatment;

[0074] 4. Vitamins, mainly vitamin E extract;

[0075] 5. Purify various active substances (natural or synthetic) to remove unwanted molecules (such as pesticides from vegetable extracts) or "residue" to obtain purified products.

[0076] 6. Processing of various natural antibacterial or antioxidant extracts, such as basil, salvia, thyme, garlic, onion, chamomile, chili powder, licorice, and fennel seeds.

[0077] Advantages of supercritical fluid extraction technology in the research and development of traditional Chinese medicine:

[0078] 1. Strong extraction ability and high extraction rate;

[0079] Supercritical CO2 extraction of active ingredients from traditional Chinese medicine (TCM) can, under optimal conditions, almost completely extract the desired components, thus significantly improving the purity and concentration of these active ingredients. Furthermore, with the continuous advancement of supercritical CO2 extraction technology and the application of perfluoropolyether ammonium carbonate (PFPE), supercritical CO2 extraction has been extended to aqueous systems, making the extraction of highly polar compounds, such as proteins, which are difficult to extract, possible.

[0080] 2. Precise control;

[0081] The extraction capacity depends on the density of the fluid, and ultimately on temperature and pressure. Changing one or both can alter the solubility, allowing for the selective separation of multiple substances in traditional Chinese medicine. This reduces impurities and highly enriches the effective components of traditional Chinese medicine, facilitating dosage reduction and quality control. Supercritical CO2 extraction allows for easy control of operating parameters, resulting in stable quality of the effective components of traditional Chinese medicine.

[0082] 3. Does not damage the active ingredients;

[0083] Supercritical CO2 has a low critical temperature and low operating temperature, which can better preserve the effective components of traditional Chinese medicine without damage or secondary degradation. Therefore, it is particularly suitable for the extraction of components that are highly sensitive to heat and easily oxidized and decomposed.

[0084] 4. Fast extraction time and short production cycle;

[0085] Once the supercritical CO2 extraction (dynamic) cycle begins, separation starts immediately. Components typically begin to precipitate within 10 minutes of extraction, and complete extraction is achieved in approximately 2–4 hours. Furthermore, no concentration step is required.

[0086] 5. Facilitates the development of new drugs;

[0087] Supercritical CO2 can also directly extract different parts of traditional Chinese medicine (TCM) from single or compound herbs, or directly extract extracts for pharmacological screening and new drug development, greatly improving the speed of new drug screening. Simultaneously, it can extract many substances that cannot be extracted using traditional methods, and it is easier to discover new components from TCM, thereby discovering new pharmacological properties and developing new drugs.

[0088] 6. Quality assurance;

[0089] Supercritical CO2 also has antioxidant and sterilization effects, which helps to ensure and improve the quality of traditional Chinese medicine.

[0090] 7. Accurate content analysis;

[0091] Supercritical fluid extraction (SFE) is a highly efficient analytical technique, often used in conjunction with GC, IR, MS, and LC. Its application in the quality analysis of traditional Chinese medicine (TCM) can objectively reflect the true content of its active ingredients.

[0092] 8. Guaranteed clinical efficacy;

[0093] Pharmacological and clinical studies have shown that supercritical CO2 extraction of traditional Chinese medicine is not only superior in terms of process, but also has stable quality and easy standard control, and its pharmacological and clinical effects can be guaranteed or even better.

[0094] 9. Cost savings;

[0095] Supercritical CO2 extraction is a simple and convenient process that saves labor and a large amount of organic solvents, and reduces pollution from waste. It undoubtedly provides a new and advanced method for the extraction, separation, preparation and concentration of traditional Chinese medicine.

[0096] Traditional Chinese medicine aging techniques:

[0097] Traditional Chinese medicine (TCM) aging is a unique method of processing medicinal materials. During the aging process, TCM is affected by external physical, chemical, and biological factors, which causes changes in its physicochemical properties, active ingredients, and functional effects. After aging, the medicinal properties are milder, the efficacy is enhanced, and the toxicity is reduced, further improving the safety, effectiveness, and quality stability of TCM, making it more in line with clinical medication needs.

[0098] The purpose of aging is mainly reflected in three aspects:

[0099] Firstly, it reduces dryness and moderates the medicinal properties, making it suitable for drugs containing a high amount of irritating components such as volatile oils;

[0100] Secondly, it enhances the efficacy of the medicine;

[0101] Third, it reduces or eliminates adverse reactions and is suitable for toxic or potent drugs.

[0102] Reduce dryness and moderate the medicinal properties:

[0103] pungent and pungent medicines containing a high amount of volatile oils are warm and easily cause dryness. After aging, their odor disappears, which can reduce their pungent, warm, and dry nature.

[0104] Enhance drug efficacy:

[0105] The aging process of traditional Chinese medicine increases the content of its active ingredients and enhances its efficacy. In Magnolia officinalis, the content of magnolol increases with the length of aging, and it also improves the antibacterial and anti-ulcer effects of the herb.

[0106] Reduce or eliminate adverse reactions:

[0107] The aging process of traditional Chinese medicine can not only change its medicinal properties and enhance its efficacy, but also significantly reduce the adverse reactions of some toxic traditional Chinese medicines after aging, thus improving the safety of clinical use.

[0108] With the influence of physical factors (light, temperature, humidity, etc.), chemical factors (oxygen, ozone, etc.), and biological factors (microorganisms, enzymes, etc.), volatile components are lost, and under the mediation of oxygen, microorganisms, enzymes, etc., compound degradation reactions, oxidation reactions, polymerization reactions, etc. occur, which leads to changes in the medicinal substances and efficacy of aged Chinese medicine.

[0109] Microbial aging technology:

[0110] Microbial transformation refers to a class or series of enzymes produced by microorganisms during metabolism that catalyze or modify substrates. Microbial transformation can combine the advantages of modern biotechnology with the strengths of traditional Chinese medicine. Through key reactions such as oxidation-reduction, glycosylation, hydroxylation, and hydrolysis, it modifies the active ingredients of Chinese medicine, producing new substances, enhancing efficacy and reducing toxicity, lowering costs, and improving bioavailability.

[0111] Biocatalysis technology can integrate the advantages of modern biotechnology with traditional Chinese medicine. Natural or artificially synthesized compounds are added to microorganisms as substrates and cultured under suitable conditions for a certain period of time, allowing the substrates to interact with the enzyme system in the whole cell, thereby producing structural changes.

[0112] After microbial aging: effective Chinese medicine components such as genes, cells, proteins, metabolic engineering and immobilized enzymes are screened, and strains are cultivated and modified to obtain microorganisms. The composition is formulated according to the traditional Chinese medicine principles of monarch, minister, assistant and guide. The composition has increased natural activity, improved Chinese medicine properties, reduced toxic side effects of certain Chinese medicines, and better intestinal absorption.

[0113] The formulation of this composition is as follows:

[0114] Principal ingredient: 5 parts of Senecio scandens extract

[0115] Astaxanthin (3 parts)

[0116] Adjuvant herbs: Artemisia capillaris 2 parts, Gardenia jasminoides 2 parts, Lygodium japonicum 2 parts, Cassia tora 2 parts

[0117] Pharmacology: 1. It can soothe the liver and clear heat, nourish yin and benefit the kidneys, regulate the spleen and stomach, and dispel wind and relieve numbness;

[0118] 2. It nourishes qi and blood, and strengthens the liver and improves eyesight;

[0119] 3. Remove obstacles and clear away blemishes;

[0120] 4. Detoxifies and relieves stress;

[0121] 5. Enhances the body's immunity;

[0122] 6. Improves intraocular pressure, regulates the optic nerve, and restores the basic refractive power of the eye axis.

[0123] Efficacy: Promotes blood circulation and removes blood stasis, promotes new blood production, nourishes yin and reduces internal heat. It is effective for symptoms such as black shadows, blurred or distorted vision, decreased or abnormal vision, blood stasis in the fundus, and lethargy. It can improve eye diseases such as myopia, amblyopia, hyperopia, cataracts, and glaucoma.

[0124] The preparation method of the complex for eye disease repair of the present invention includes the following steps:

[0125] S1: Select fresh, high-quality Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora for the first aging process;

[0126] S2: The once aged Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora seeds were subjected to supercritical extraction to obtain high-purity extracts.

[0127] S3: The supercritical extraction extracts of Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora were placed into a reaction vessel for a second microbial aging treatment.

[0128] S4: The extracts of Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora after the second aging were synthesized into a complex according to the principles of traditional Chinese medicine.

[0129] S5: The complex is placed in a reactor for a third organic aging process to obtain the Xinsenmai compound Tianmu complex.

[0130] The extraction in steps S1 to S5 all employs supercritical fluid extraction technology. Extracts obtained using this technology have a large molecular weight and contain standard active ingredients, and are free of harmful components such as pesticide residues and heavy metals.

[0131] The aging time in steps S1, S3, and S5 is 5 to 7 months.

[0132] During the aging process in step S1, the temperature is 28–40°C and the relative humidity is 75%–85%.

[0133] The compositions of the present invention can also be used to prepare soft capsule dosage forms:

[0134] Based on the complex obtained from ultra-precipitated extraction, it was formulated into a soft capsule dosage form. Soft capsules are a convenient dosage form for medication, easily absorbed and digested.

[0135] Modern Chinese medicine fermentation technology mainly relies on the biotransformation of microorganisms. During the growth and metabolism of microorganisms, various enzymes such as cellulase, ligninase, and lipase are produced under the action of probiotics. Enzymes are highly efficient biological catalysts that enable complex biochemical reactions to be completed rapidly at room temperature and pressure. Microorganisms have a strong ability to decompose and transform, and can produce abundant secondary metabolites.

[0136] Clinical efficacy:

[0137] 1. Patient: Hu, male, 10 years old, underwent ophthalmological screening at Sichuan Provincial People's Hospital Jinniu Branch on February 9, 2023. Clinical diagnosis: refractive error, myopia, astigmatism. Left eye astigmatism 1.00, myopia 2.00, oily smoke astigmatism 1.25, myopia 1.75, and wears corrective glasses;

[0138] After taking this compound formula for 4 months without wearing glasses, a follow-up examination on July 9, 2023 showed that the left eye was 55 degrees with myopia of 80 degrees and the right eye was 58 degrees with astigmatism of 75 degrees. The vision had basically recovered.

[0139] 2. Patient: Xin Xin, 12 years old. After taking this compound formula in February 2023, her uncorrected visual acuity improved from 0.2 in the right eye and 0.4 in the left eye to normal visual acuity of 0.8 in the right eye and 0.8 in the left eye in 3 months.

[0140] 3. Patient: Xixi, 10 years old, started taking this compound formula in March 2023. In July 2023, during a follow-up examination, her uncorrected visual acuity improved from 0.12 in the right eye and 0.12 in the left eye to 0.6 in the right eye and 0.8 in the left eye.

[0141] 4. Patient: Leilei, female, 13 years old. After taking the compound formula for 3 and a half months, her uncorrected visual acuity improved from 0.3 in the right eye and 0.3 in the left eye to normal visual acuity of 0.8 in the right eye and 1.0 in the left eye.

[0142] The technical solutions of the present invention are not limited to the specific embodiments described above. Any technical modifications made in accordance with the technical solutions of the present invention fall within the protection scope of the present invention.

Claims

1. A complex for repairing eye diseases, characterized in that: It is synthesized from extracts of Senecio scandens, astaxanthin, Artemisia capillaris, Gardenia jasminoides, Lygodium japonicum, and Cassia tora.

2. The complex for eye disease repair according to claim 1, characterized in that: By weight, it contains 5 parts of the Senecio scandens extract, 3 parts of the astaxanthin, 2 parts of Artemisia capillaris, 2 parts of Gardenia jasminoides, 2 parts of Lygodium japonicum, and 2 parts of Cassia tora.

3. The complex for eye disease repair according to claim 1, characterized in that: The Senecio scandens extract is extracted from Senecio scandens, the astaxanthin extract is extracted from Haematococcus pluvialis, the Artemisia capillaris extract is extracted from Artemisia capillaris, the Gardenia jasminoides extract is extracted from Gardenia jasminoides, the Lygodium japonicum extract is extracted from the sporangia of the leaves of Lygodium japonicum, and the Cassia tora extract is extracted from Cassia tora. All of these extraction processes employ supercritical fluid extraction technology.

4. The complex for eye disease repair according to claim 1, characterized in that: The *Senecio scandens* and *Haematococcus pluvialis* were aged in a reaction vessel before being extracted from *Artemisia capillaris*, *Gardenia jasminoides*, *Gynostemma pentaphyllum*, and *Cassia tora*.

5. A method for preparing a complex for eye disease repair as described in claim 1 or 2, characterized in that, Includes the following steps: S1: Select fresh, high-quality Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora for the first aging process; S2: The once aged Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora seeds were subjected to supercritical extraction to obtain high-purity extracts. S3: The supercritical extraction extracts of Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora were placed into a reaction vessel for a second microbial aging treatment. S4: The extracts of Senecio scandens, Haematococcus pluvialis, Artemisia capillaris, Gardenia jasminoides, Lysimachia christinae, and Cassia tora after the second aging were synthesized into a complex according to the principles of traditional Chinese medicine. S5: The complex is placed in a reactor for a third organic aging process to obtain the Xinsenmai compound Tianmu complex.

6. The method for preparing the complex for eye disease repair according to claim 5, characterized in that: The extraction in steps S1 to S5 all employs supercritical extraction technology.

7. The method for preparing the complex for eye disease repair according to claim 5, characterized in that: The aging time in steps S1, S3, and S5 is 5 to 7 months.

8. The method for preparing the complex for eye disease repair according to claim 5, characterized in that: During the aging process in step S1, the temperature is 28–40°C and the relative humidity is 75%–85%.