Monoclonal antibody combination specifically recognizing hemagglutinin protein of avian influenza virus h6 subtype and use thereof

CN122103323AActive Publication Date: 2026-05-29BEIJING SUBENYUANHE BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING SUBENYUANHE BIOTECHNOLOGY CO LTD
Filing Date
2026-04-29
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing detection methods for the H6 subtype of avian influenza virus suffer from cross-reactivity interference, leading to false positives and making it difficult to achieve a balance between specificity and sensitivity. In particular, there is a lack of effective means in field screening and epidemiological surveillance.

Method used

A combination of monoclonal antibodies that specifically recognize the hemagglutinin protein of the H6 subtype of avian influenza virus, including monoclonal antibodies 2D12 and 4G3, was used to construct colloidal gold test strips targeting different epitopes for rapid and convenient in vitro detection.

Benefits of technology

It improves the accuracy and sensitivity of detection, avoids cross-reaction with other influenza subtypes such as H1, H5, H7, and H9, as well as common avian pathogens, and is suitable for on-site screening and early detection of H6 subtype avian influenza virus.

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Abstract

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for specifically recognizing hemagglutinin protein of avian influenza virus H6 subtype and a use thereof. The antibody combination is composed of monoclonal antibodies 2D12 and 4G3, is directed to different epitopes of the H6 hemagglutinin protein, has clear heavy chain and light chain variable region complementarity determining region sequences, and is respectively shown as SEQ ID NO. 1-12. The antibody combination has high specificity and affinity, and can effectively avoid cross reactions of other influenza subtypes such as H1, H5, H7 and H9 and common avian pathogens. A colloidal gold detection test strip based on the antibody combination has high sensitivity and simple operation, is suitable for in vitro detection and epidemiological screening of the H6 subtype avian influenza virus, solves the false positive problem caused by cross reactions in the prior art, and provides a reliable technical means for monitoring and quarantine of the H6 subtype virus.
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Citation Information

Patent Citations

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