A method for constructing a preeclampsia animal model based on down-regulation of HAPLN1 to induce abnormal placental cell aging and application thereof

By injecting pregnant mice with Ad-shHAPLN1 virus to downregulate HAPRN1 expression, an animal model of preeclampsia was constructed. This solved the problem that existing models could not reflect placental cell aging, and enabled the simulation of key pathological processes in preeclampsia, providing a research tool that is closer to the essence of the disease.

CN122104811AActive Publication Date: 2026-05-29THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
Filing Date
2026-04-13
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing animal models of preeclampsia are insufficient to effectively reflect the core pathological process of placental cell aging, leading to inadequate research on the disease mechanism.

Method used

By injecting pregnant mice with Ad-shHAPLN1 virus to downregulate HAPLN1 expression levels, an animal model of preeclampsia that can simulate abnormal placental cell aging was constructed, and relevant pathological indicators were monitored to evaluate the establishment of the model.

Benefits of technology

It successfully reproduced the main clinical manifestations of preeclampsia, including persistent high blood pressure, significant proteinuria, multiple organ damage, and intrauterine growth restriction in fetal mice, and dynamically simulated placental cell aging, thus having stronger pathological representativeness and practicality.

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Abstract

The application discloses a kind of based on HAPLN1 down-regulation induced preeclampsia animal model construction method and application of placental cell abnormal aging, belong to the field of biological medicine technology.The application can stably induce the animal model with typical preeclampsia clinical characteristics by reducing the expression level of HAPLN1 in pregnant mice, while reproducing the abnormal phenotype of placental cell aging.The method has strong controllability in operation process, and the model has good reproducibility, which can provide effective experimental tools for in-depth study of the pathogenesis of preeclampsia and development of new diagnosis and treatment strategies.
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Citation Information

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