Amino acid binding agents and uses thereof
By developing a variety of binding agents from the binding agent library, the problems of insufficient sensitivity and selectivity in the detection of amino acids in the existing technology have been solved, and high specificity and high affinity binding to a variety of amino acids has been achieved, thereby improving the detection efficiency.
Patent Information
- Application Number
- CN202480069383.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-10-11
- Filing Date
- 2024-09-20
- Publication Date
- 2026-05-29
AI Technical Summary
Existing binding agents lack sufficient sensitivity and selectivity when detecting single monomeric amino acids, making it difficult to efficiently identify and specifically bind multiple amino acids or their derivatives.
A binding agent library containing various binding agents, such as antibodies, antibody fragments, and engineered scFv, was developed. This library can specifically recognize and bind to various amino acids or their derivatives. Affinity maturation was performed using methods such as yeast surface display and phage display. The binding agent library contains polyclonal and monoclonal antibodies, and detection was performed using ELISA, surface plasmon resonance, and biofilm interferometry.
It achieves highly specific and high-affinity binding to a variety of amino acids or their derivatives, and can recognize and bind to specific amino acids at low concentrations, thus improving the sensitivity and selectivity of detection.