A method for iso5 level clean hatching of spf ducklings

Abstract

The application provides an ISO5-level clean hatching method for SPF ducks and belongs to the technical field of animal breeding equipment.The method solves the problems of the traditional hatching equipment, such as insufficient cleanliness, high pollution risk, poor environmental control precision and the inability to meet the biological safety and sterile breeding requirements of SPF ducks.The method comprises the following steps: 1: introducing air from the outside and sequentially performing three-stage progressive purification treatment on the introduced air through a multi-stage air inlet filtering structure (9) of a hatcher; 2: sending the three-stage purified clean air into a hatching bin (1) of the hatcher in a vertical laminar flow mode; 3: sending an egg tray vehicle (13) carrying SPF duck eggs into the hatching bin (1); and 4: operating personnel operate the SPF duck eggs in the hatching bin (1) through a glove operation system (4) installed on the hatching bin (1) under the condition of maintaining the positive pressure and cleanliness in the hatching bin (1). The method is mainly used for the breeding of poultry, such as SPF ducks, which do not carry specific pathogenic bacteria.

Description

Technical Field

[0001] This invention belongs to the field of animal breeding equipment technology, and in particular relates to an ISO 5 grade purification hatching method for SPF ducks. Background Technology

[0002] SPF ducks refer to ducks that have undergone rigorous screening to ensure they do not carry specific pathogens. SPF ducks are indispensable experimental animals and core supporting materials in agricultural science research on waterfowl diseases and in the development, production, and testing of waterfowl biological products such as vaccines. The hatching stage of SPF ducks is the most vulnerable stage in their early life and the most critical point for the prevention and control of microbial contamination, directly affecting the biosafety and purity of the entire population. Currently, traditional poultry hatching equipment is mainly designed for ordinary poultry farming and has the following defects: it lacks a high-efficiency air filtration device, and the cleanliness of the equipment cannot be precisely controlled, falling far short of the ISO 5 level purification standard required for SPF animal husbandry; it lacks a continuous and stable positive pressure guarantee, making it easy for external pollutants to enter, and cross-contamination can easily occur inside, leading to embryonic death or ducklings carrying the virus; the equipment materials are not designed for sterility, resulting in unsanitary corners and a lack of integrated control with full traceability. Summary of the Invention

[0003] In view of this, the present invention aims to propose an ISO 5-level purification hatching method for SPF ducks, in order to solve the problems of insufficient cleanliness, high risk of contamination, poor environmental control precision, and inability to meet the biosafety and aseptic breeding requirements of SPF ducks in traditional hatching equipment.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a method for ISO 5 grade purification hatching of SPF ducks, comprising the following steps: Step 1: Air is introduced from the outside and undergoes a three-stage progressive purification process through the multi-stage filtration air intake structure of the brooder. Step 2: The clean air after the three-stage purification process is sent into the hatching chamber of the hatcher in a vertical laminar flow manner, and the positive pressure in the hatching chamber is maintained at 15Pa to 20Pa. The air exchange rate is not less than 50 times per hour. The temperature in the hatching chamber is controlled at 37.1℃±0.1℃ and the humidity is within the range of 65%±2% to 75%±2%. Step 3: The egg tray carrying SPF duck eggs is sent into the hatching chamber through the pass-through window system; Step 4: The operator operates the system through gloves installed on the hatching chamber, and handles the SPF duck eggs in the hatching chamber while maintaining positive pressure and cleanliness. Step 5: The hatched ducklings are transferred to the purification isolator system via a transfer window system for rearing.

[0005] Furthermore, the hatcher also includes a multi-stage filtration air outlet structure and an ultraviolet sterilization component. The air inlet at the top of the hatching chamber is connected to the multi-stage filtration air inlet structure, and the air outlet at the bottom of the hatching chamber is connected to the multi-stage filtration air outlet structure through an air outlet pipe. The egg tray is placed inside the hatching chamber. An ultraviolet sterilization component is installed on one side of the hatching chamber. The air inlet end of the multi-stage filtration air inlet structure is connected to the air inlet, and the air outlet end of the multi-stage filtration air outlet structure is connected to the air outlet. The multi-stage filtration air inlet structure includes a primary air inlet filter, a secondary air inlet filter, and a tertiary air inlet filter arranged sequentially along the air supply direction. The multi-stage filtration air outlet structure includes a primary air outlet filter and a secondary air outlet filter arranged sequentially along the air exhaust direction. A stable positive pressure of 15-20 Pa is maintained inside the hatching chamber.

[0006] Furthermore, the air outlet of the secondary filter is provided with an activated carbon filter layer.

[0007] Furthermore, a first fan is provided between the activated carbon filter layer and the exhaust port.

[0008] Furthermore, an electric heating device is provided between the air inlet and the primary air filter at the air inlet end, and a humidifier is provided between the tertiary air filter at the air inlet end and the air inlet end of the air inlet pipe.

[0009] Furthermore, a second fan is provided between the secondary air inlet filter and the tertiary air inlet filter.

[0010] Furthermore, the first fan, electric heating device, humidifier, and second fan are all connected to a digital control panel. The hatching chamber is equipped with a temperature and humidity probe, which transmits the detected temperature and humidity to the control system within the digital control panel.

[0011] Furthermore, the transfer window system includes a transfer window, a first sealing door, and a second sealing door. The transfer window is installed on the side of the hatching chamber away from the ultraviolet sterilization component. The second sealing door and the first sealing door are respectively installed at the connection between the transfer window and the hatching chamber and at the outlet.

[0012] Furthermore, the egg tray cart is evenly distributed with multiple layers of detachable egg trays.

[0013] Furthermore, four glove operating systems are provided on both sides of the hatching chamber.

[0014] Furthermore, the hatching chamber is made of 16L stainless steel.

[0015] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention uses a three-stage progressive air filtration system, combined with vertical laminar flow and more than 50 air changes per hour, to ensure that the core area of ​​the hatching chamber consistently reaches ISO 5 cleanliness, providing the highest level of air purification guarantee for SPF duck hatching.

[0016] 2. The hatching chamber of this invention maintains a stable positive pressure of 15-20 Pa, forming an effective air barrier to prevent the intrusion of unpurified air from the outside. At the same time, the exhaust side is also highly efficient filtered to prevent internal pollutants from leaking out, thus achieving two-way biological isolation.

[0017] 3. The main body of the hatching chamber of this invention is made of 316L stainless steel with seamless welding and mirror polishing, with no dead corners for hygiene, making it easy to clean and disinfect. Combined with egg trays that can be sterilized by high temperature and high pressure, a built-in ultraviolet sterilization system, as well as isolation operation gloves and a transfer window, a complete sterile system from hardware to operation process is constructed.

[0018] 4. This invention achieves precise closed-loop control of key environmental parameters such as temperature, humidity, and pressure difference through a control system, meeting the stringent requirements for SPF duck embryo development. All operating data is automatically recorded, meeting the traceability requirements of GMP (Good Manufacturing Practice) for pharmaceuticals and GLP (Good Laboratory Practice). Attached Figure Description

[0019] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings: Figure 1 This is a schematic diagram of an ISO 5-level purification hatching method for SPF ducks according to the present invention. Figure 2 This is a cross-sectional schematic diagram of an ISO 5-level purification hatching method for SPF ducks according to the present invention. In the picture: 1- Hatching chamber, 2- Pass-through window, 3- First sealed door, 4- Glove operating system, 5- Air outlet duct, 6- Multi-stage filtration air outlet structure, 7- Exhaust vent, 8- Air inlet, 9- Multi-stage filtration air inlet structure, 10- Air inlet duct, 11- Air supply vent, 12- Second sealed door, 13- Egg tray cart, 14- Air outlet, 15- Ultraviolet sterilization component, 16- Primary air outlet filter, 17- Secondary air outlet filter, 18- Activated carbon filter layer, 19- First fan, 20- Electric heating device, 21- Humidifier, 22- Primary air inlet filter, 23- Secondary air inlet filter, 24- Secondary fan, 25- Tertiary air inlet filter, 26- Digital control panel, 27- Temperature and humidity probe. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of the present invention can be combined with each other, and the described embodiments are only some embodiments of the present invention, not all embodiments.

[0021] Detailed Implementation Method 1: See Figure 1-2 This embodiment describes a method for raising SPF ducklings with ISO 5-level purification, comprising the following steps: Step 1: Air is introduced from the outside and undergoes a three-stage progressive purification process through the multi-stage filtration air intake structure 9 of the brooder. Step 2: The clean air after the three-stage purification process is sent into the hatching chamber 1 of the hatcher in a vertical laminar flow manner, and the positive pressure in the hatching chamber 1 is maintained at 15Pa to 20Pa. The air exchange rate is not less than 50 times per hour. The temperature in the hatching chamber 1 is controlled at 37.1℃±0.1℃ and the humidity is within the range of 65%±2% to 75%±2%. Step 3: Send the egg tray 13 carrying SPF duck eggs into the hatching chamber 1; Step 4: The operator operates the system 4 through the gloves installed on the hatching chamber 1 to operate the SPF duck eggs in the hatching chamber 1 while maintaining positive pressure and cleanliness. Step 5: The hatched ducklings are transferred to the purification isolator system via a transfer window system for rearing.

[0022] Furthermore, the hatcher also includes a multi-stage filtration air outlet structure 6 and an ultraviolet sterilization component 15. The air outlet 11 at the top of the hatching chamber 1 is connected to the multi-stage filtration air inlet structure 9, and the air outlet 14 at the bottom of the hatching chamber 1 is connected to the multi-stage filtration air outlet structure 6 through an air outlet pipe 5. The egg tray 13 is installed inside the hatching chamber 1, and an ultraviolet sterilization component 15 is installed on one side inside the hatching chamber 1. The air inlet end of the multi-stage filtration air inlet structure 9 is connected to the air inlet 8 through an air inlet pipe 10, and the air outlet end of the multi-stage filtration air outlet structure 6 is connected to the air outlet 7. The multi-stage filtration air inlet structure 9 includes an air inlet primary filter 22, an air inlet secondary filter 23, and an air inlet tertiary filter 25 arranged sequentially along the air supply direction. The multi-stage filtration air outlet structure 6 includes an air outlet primary filter 16 and an air outlet secondary filter 17 arranged sequentially along the air exhaust direction.

[0023] The hatching chamber 1 has an air inlet 11 at the top and an air outlet 14 at the bottom. External air is drawn in through the air inlet 8 and flows sequentially through the electric heating device 20 and humidifier 21 integrated on the air inlet pipe 10 for initial temperature and humidity regulation. Subsequently, the air enters the multi-stage filtration air inlet structure 9 and passes sequentially through the first-stage filter 22 (e.g., a G4 pre-filter), the second-stage filter 23 (e.g., an F8 medium-efficiency filter), and is driven by the second fan 24. Finally, it undergoes final purification through the third-stage filter 25 (e.g., an H14 high-efficiency filter). After these three stages of filtration, the air that reaches ISO 5 cleanliness is delivered into the hatching chamber 1 from the air inlet 11 in a vertical laminar flow manner.

[0024] Inside the hatching chamber 1, clean air flows evenly from top to bottom, providing a stable and clean incubation environment for the SPF duck eggs on the egg tray 13. The egg tray 13 is equipped with multiple removable egg trays for easy loading and unloading. After use, the air maintains positive pressure inside the chamber and enters the multi-stage filtration air outlet structure 6 from the bottom air outlet 14 through the air outlet pipe 5. In this structure, the air first passes through the primary air outlet filter 16, and then through the secondary air outlet filter 17, such as the H14 high-efficiency filter, to prevent any biological particles from being discharged from the equipment. Subsequently, the air can pass through an activated carbon filter layer 18 to absorb odors, and finally, driven by the first fan 19, it is safely discharged from the exhaust port 7.

[0025] The inner wall of one side of the hatching chamber 1 is equipped with an ultraviolet sterilization component 15, which can disinfect the space inside the chamber before and after hatching. The outer wall of the other side is equipped with a transfer window 2, which is equipped with a second sealing door 12 and a first sealing door 3. Both sealing doors are equipped with sealing strips around them to realize the transfer of materials under sterile conditions. Multiple glove operating systems 4 can also be installed on both sides of the hatching chamber 1. The glove operating system 4 refers to flexible gloves that are fixedly and sealed on the side of the hatching chamber 1. Operators can put their hands into these gloves to operate the inside of the equipment without opening the equipment or damaging the clean environment inside.

[0026] The entire equipment, including the first fan 19, the second fan 24, the electric heating device 20, the humidifier 21, etc., is automatically controlled and monitored by the control system to ensure accurate and stable environmental parameters. All data can be recorded and exported. The control system achieves fully automatic closed-loop control of temperature and humidity ±0.1℃ / ±2%, pressure difference, filtration resistance, and sterilization process.

[0027] Among them, the primary air inlet filter 22 adopts a G4 pre-filter to intercept hair and dust and protect the downstream filter element; the secondary air inlet filter 23 adopts an F8 medium-efficiency filter to filter aerosols and particulate carriers; and the tertiary air inlet filter 25 adopts an H14 high-efficiency filter to achieve ISO 5 cleanliness.

[0028] An electric heating device 20 is installed between the air inlet 8 and the primary air inlet filter 22, and a humidifier 21 is installed between the tertiary air inlet filter 25 and the air inlet end of the air inlet pipe 10. The humidifier adjusts the air before it enters the temperature and humidity sensitive tertiary air inlet filter 25, avoiding the risk of uneven local temperature and humidity, water droplets, or condensation that may occur when adjusting the air directly inside the hatching chamber 1. Heating the air can reduce its relative humidity, especially in cold seasons, preventing the high-efficiency filter from becoming damp and failing due to the intake of high-humidity air, and extending the service life of the core filter components. The pre-treated air is close to the set parameters when it enters the hatching chamber. Combined with possible fine-tuning inside the chamber, it can reach and maintain the precise temperature and humidity required for hatching more quickly and smoothly, with the temperature being 37.1℃±0.1℃ and the humidity being 65-75%±2%.

[0029] The transfer window system includes a transfer window 2, a first sealing door 3, and a second sealing door 12. The transfer window 2 is installed on the side of the hatching chamber 1 away from the ultraviolet sterilization component 15. The second sealing door 12 and the first sealing door 3 are installed at the connection between the transfer window 2 and the hatching chamber 1 and at the outlet, respectively. Without damaging the ISO5 clean environment and positive pressure state inside the hatching chamber 1, the system can safely transfer hatching trays, tools, and other items. The two-layer door structure effectively eliminates the risk of external contaminants directly entering the core clean area with the airflow when transferring items.

[0030] Among them, the air exchange rate in hatching chamber 1 is ≥50 times / h, and the positive pressure inside the chamber is maintained at 15 Pa to 20 Pa.

[0031] The specific embodiments of the present invention disclosed above are merely illustrative of the invention. These embodiments do not exhaustively describe all details, nor do they limit the invention to the specific embodiments described. Many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention.

Claims

1. A method for ISO 5 grade purification hatching of SPF ducks, characterized in that: Includes the following steps: Step 1: Air is introduced from the outside and undergoes a three-stage progressive purification process through the multi-stage filtration air intake structure (9) of the hatcher. Step 2: The clean air after the three-stage purification process is sent into the hatching chamber (1) of the hatcher in a vertical laminar flow manner, and the positive pressure in the hatching chamber (1) is maintained at 15Pa to 20Pa. The air exchange rate is not less than 50 times per hour. The temperature in the hatching chamber (1) is controlled at 37.1℃±0.1℃ and the humidity is within the range of 65%±2% to 75%±2%. Step 3: The egg tray (13) carrying SPF duck eggs is sent into the hatching chamber (1) through the transfer window system; Step 4: The operator operates the SPF duck eggs in the hatching chamber (1) by operating the system (4) through the gloves installed on the hatching chamber (1) while maintaining the positive pressure and cleanliness inside the hatching chamber (1); Step 5: The hatched ducklings are transferred to the purification and isolation system via a pass-through window system for feeding. keep.

2. The method for ISO 5 grade purification hatching of SPF ducks according to claim 1, characterized in that: The hatcher also includes a multi-stage filtration air outlet structure (6) and an ultraviolet sterilization component (15). The air outlet (11) at the top of the hatching chamber (1) is connected to the multi-stage filtration air inlet structure (9) through an air inlet pipe (10). The air outlet (14) at the bottom of the hatching chamber (1) is connected to the multi-stage filtration air outlet structure (6) through an air outlet pipe (5). The egg tray (13) is set inside the hatching chamber (1). An ultraviolet sterilization component (15) is installed on one side inside the hatching chamber (1). The multi-stage filtration... The air inlet end of the air inlet structure (9) is connected to the air inlet (8), and the air outlet end of the multi-stage filtration air outlet structure (6) is connected to the air outlet (7). The multi-stage filtration air inlet structure (9) includes an air inlet primary filter (22), an air inlet secondary filter (23), and an air inlet tertiary filter (25) arranged sequentially along the air supply direction. The multi-stage filtration air outlet structure (6) includes an air outlet primary filter (16) and an air outlet secondary filter (17) arranged sequentially along the air exhaust direction.

3. The method for ISO 5 grade purification hatching of SPF ducks according to claim 2, characterized in that: The outlet end of the secondary filter (17) is provided with an activated carbon filter layer (18).

4. The method for ISO 5 grade purification hatching of SPF ducks according to claim 3, characterized in that: A first fan (19) is provided between the activated carbon filter layer (18) and the exhaust port (7).

5. The method for ISO 5 grade purification hatching of SPF ducks according to claim 4, characterized in that: An electric heating device (20) is provided between the air inlet (8) and the primary air inlet filter (22), and a humidifier (21) is provided between the tertiary air inlet filter (25) and the air inlet of the air inlet pipe (10).

6. The method for ISO 5 grade purification hatching of SPF ducks according to claim 5, characterized in that: A second fan (24) is provided between the secondary air inlet filter (23) and the tertiary air inlet filter (25).

7. The method for ISO 5 grade purification hatching of SPF ducks according to claim 6, characterized in that: The first fan (19), electric heating device (20), humidifier (21) and second fan (24) are all connected to the digital control panel (26). The hatching chamber (1) is equipped with a temperature and humidity probe (27), which transmits the detected temperature and humidity to the control system in the digital control panel (26).

8. The method for ISO 5 grade purification hatching of SPF ducks according to claim 2, characterized in that: The transfer window system includes a transfer window (2), a first sealing door (3), and a second sealing door (12). The transfer window (2) is installed on the side of the hatching chamber (1) away from the ultraviolet sterilization component (15). The second sealing door (12) and the first sealing door (3) are installed at the connection between the transfer window (2) and the hatching chamber (1) and at the outlet, respectively.

9. A method for ISO 5 grade purification hatching of SPF ducks according to claim 2, characterized in that: The egg tray cart (13) is evenly distributed with multiple layers of detachable egg trays.

10. A method for ISO 5 grade purification hatching of SPF ducks according to claim 2, characterized in that: The hatching chamber (1) is equipped with four glove operating systems (4) on both sides.

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