A solid waste agricultural enzyme for a fish-vegetable symbiotic system and a preparation method and application thereof

Abstract

The application provides a fish-mudgrowing system solid waste agricultural enzyme and a preparation method and application thereof, and belongs to the technical field of waste recycling. The bottom mud, dead fish and tail vegetables of a fish-mudgrowing system are mixed, a compound microbial inoculum and a carbon source are added, and anaerobic fermentation is carried out to obtain the agricultural enzyme, which can be directly used in a planting bed. The application not only realizes resource utilization of all solid waste of the fish-mudgrowing system, but also promotes plant growth and enhances the disease resistance of plants.

Description

Technical Field

[0001] This invention belongs to the field of waste recycling technology, and in particular relates to an agricultural enzyme made from solid waste in an aquaponics system, its preparation method, and its application. Background Technology

[0002] Aquaponics, an ecological circular agriculture model combining aquaculture and hydroponics, transforms fish metabolic waste in the aquaculture water into nutrients that plants can absorb. While absorbing nutrients, the plants purify the water, and the purified water is then returned to the aquaculture ponds. The system achieves a water resource recycling rate exceeding 90%, significantly saving water. Nitrogen utilization rate increases from 30% in traditional agriculture to 85%, and yield per unit area increases by 3 to 5 times. It also eliminates problems such as soil compaction and eutrophication caused by the overuse of chemical fertilizers and pesticides in traditional agriculture. However, there is currently no suitable method for treating solid waste such as uneaten feed, feces, dead fish, and vegetable waste in aquaponics systems. Summary of the Invention

[0003] In view of this, the purpose of this invention is to provide an agricultural enzyme from solid waste of an aquaponics system, its preparation method and application, in which all solid waste is fermented with probiotics to prepare agricultural enzyme, which is then returned to the planting system, truly achieving zero emissions.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides an agricultural enzyme from solid waste of an aquaponics system, comprising bottom mud, dead fish, and vegetable waste, wherein the mass ratio of bottom mud, dead fish, and vegetable waste is 2~4:2~4:1~2, and the bottom mud, dead fish, and vegetable waste are all produced by the aquaponics system.

[0005] Preferably, it also includes 2-3% carbon source of the total mass of bottom mud, dead fish, and vegetable waste, and 3-10% compound microbial agent of the total mass of bottom mud, dead fish, and vegetable waste.

[0006] Preferably, the compound microbial agent includes Lactobacillus hessei, Bacillus subtilis, and Candida utilis, wherein the mass ratio of Lactobacillus hessei, Bacillus subtilis, and Candida utilis is 1:1:1.

[0007] Preferably, the initial viable bacterial concentration of the *Lactobacillus hesslerii* is 5.0~7.2 × 10⁻⁶. 8 The initial viable concentration of Bacillus subtilis was 1.0~1.2×10 CFU / mL. 9 The initial viable concentration of *C. utilis* was 1.0–1.5 × 10⁻⁶ CFU / mL. 8 CFU / mL.

[0008] Preferably, the carbon source includes molasses.

[0009] This invention also provides a method for preparing agricultural enzymes from solid waste of the aforementioned aquaponics system, comprising the following steps: (1) Bottom sediment was captured from the aquaponics system; (2) Collect dead fish and vegetable waste from the aquaponics system, crush the dead fish into fish paste, and mix the vegetable waste and water in equal proportions and crush them to obtain vegetable waste juice. (3) Mix the bottom mud, fish mud, vegetable juice, carbon source, and compound microbial agent, and ferment to obtain agricultural enzymes from solid waste of aquaponics system.

[0010] Preferably, the fermentation temperature in step (3) is 25~35℃ and the fermentation time is 25~35 days.

[0011] The present invention also provides the application of agricultural enzymes from the solid waste of the aquaponics system in inhibiting plant pathogens.

[0012] Preferably, the plant pathogens include Alternaria solanacearum and Fusarium oxysporum.

[0013] This invention also provides the application of agricultural enzymes derived from solid waste in aquaponics systems in vegetable cultivation within aquaponics systems.

[0014] Compared with the prior art, the present invention has the following beneficial effects: (1) The agricultural enzyme of the present invention has the characteristics of good fermentation performance and stable function. Most existing agricultural enzymes are naturally fermented, while the present invention, by inoculating Lactobacillus schrei, Bacillus subtilis and Candida utilis, not only greatly shortens the enzyme preparation cycle, but also helps the enzyme to play a stable role in production.

[0015] (2) The agricultural enzyme of the present invention has comprehensive functions. The agricultural enzyme of the present invention uses bottom mud, dead fish and vegetable waste as fermentation raw materials, with high nutrient content and high effective live bacteria concentration after fermentation; Lactobacillus russei RX strain has strong antibacterial effect and probiotic function against a variety of aquaculture bacteria and pathogens, and Bacillus subtilis K8 has inhibitory effect on a variety of plant pathogenic fungi. Therefore, the agricultural enzyme produced by the present invention can be directly used in planting beds, which can not only provide nutrients for plant growth and promote plant growth, but also have a certain inhibitory effect on plant pathogens in the system.

[0016] (3) This invention achieves true zero emissions and ultra-high resource efficiency. Through microbial fermentation, solid waste such as bottom mud, dead fish, and vegetable waste in the aquaponics system is transformed into agricultural enzymes. Nutrients (nitrogen, phosphorus, potassium, etc.) in the bottom mud and vegetable waste and protein in the dead fish are transformed into nutrients that can be absorbed by plants, realizing 100% internal recycling of solid waste in the system and completely solving the environmental pollution problem of solid waste. Attached Figure Description

[0017] Figure 1 It is the inhibition rate of enzymes against cucumber wilt and tomato early blight; Figure 2 This is a plate graph showing the inhibitory effect of enzymes on plant pathogens. Detailed Implementation

[0018] This invention provides an agricultural enzyme for solid waste from an aquaponics system, comprising bottom mud, dead fish, and vegetable waste. The preferred mass ratio of the bottom mud, dead fish, and vegetable waste is 2~4:2~4:1~2, and more preferably 2:1:1.

[0019] In this invention, the bottom mud, dead fish, and vegetable waste are all produced by the aquaponics system; The sediment is the sediment captured by the microfilter and arc screen in the aquaponics system; The fish paste mentioned refers to dead fish produced during the production process of the aquaponics system; The vegetable waste juice mentioned is the vegetable waste produced by the aquaponics system.

[0020] In this invention, the present invention also includes a carbon source of 2-3% of the total mass of bottom mud, dead fish, and vegetable waste, and a compound microbial agent of 3-10% of the total mass of bottom mud, dead fish, and vegetable waste. The preferred amount of carbon source added is 2.5%, and the carbon source includes molasses; The preferred amount of the compound microbial agent is 10%. The compound microbial agent includes Lactobacillus hessei, Bacillus subtilis, and Candida utilis, with the mass ratio of Lactobacillus hessei, Bacillus subtilis, and Candida utilis being 1:1:1.

[0021] In this invention, *Lactobacillus hessler* is prepared into a *Lactobacillus hessler* bacterial suspension. The preparation method of the *Lactobacillus hessler* bacterial suspension includes the following steps: inoculating *Lactobacillus hessler* into MRS medium for culture to obtain *Lactobacillus hessler* bacterial suspension; the MRS medium uses water as a solvent and includes the following components at the following concentrations: brown sugar 10-20 g / L, preferably 15 g / L; yeast extract 5-15 g / L, preferably 8 g / L; sodium acetate 2-10 g / L, preferably 5 g / L; diammonium citrate 1-5 g / L, preferably 2 g / L; manganese sulfate 0.01-0.05 g / L, preferably 0.02 g / L; potassium dihydrogen phosphate 1-4 g / L, preferably 2 g / L; the culture temperature is 25-40℃, preferably 35℃; the culture time is 18-36 h, preferably 24 h; the initial viable cell concentration of *Lactobacillus hessler* is preferably 5.0-7.2 × 10⁻⁶. 8 CFU / mL, further preferably 7.2 × 10⁻⁶. 8 CFU / mL; In this invention, Bacillus subtilis is prepared into a Bacillus subtilis bacterial suspension. The preparation method of Bacillus subtilis includes the following steps: inoculating Bacillus subtilis into GLY medium and culturing to obtain a Bacillus subtilis bacterial suspension; the GLY medium uses water as a solvent and includes the following components at the following concentrations: soluble starch 7-15 g / L, preferably 10 g / L; peptone 7-15 g / L, preferably 10 g / L; sodium chloride 2-5 g / L, preferably 3 g / L; calcium carbonate 0.5-1.5 g / L, preferably 1 g / L; magnesium sulfate 0.5-1.5 g / L, preferably 1 g / L; dipotassium hydrogen phosphate 0.5-2 g / L, preferably 1 g / L; the culture temperature is 25-40℃, preferably 37℃; the culture time is 18-36 h, preferably 32 h; the initial viable cell concentration of Bacillus subtilis is preferably 1.0-1.2 × 10⁻⁶. 9 CFU / mL, further preferably 1.2 × 10⁻⁶ 9 CFU / mL.

[0022] In this invention, *Candida utilis* is prepared into a *Candida utilis* culture medium. The preparation method of *Candida utilis* includes the following steps: inoculating *Candida utilis* strains into YPD medium and culturing to obtain *Candida utilis* culture medium; the YPD medium uses water as a solvent and includes the following components at the following concentrations: yeast extract 7-15 g / L, preferably 10 g / L; peptone 7-15 g / L, preferably 10 g / L; glucose 15-25 g / L, preferably 20 g / L; the culture temperature is 25-30℃, preferably 28℃; the culture time is 24-48 h, preferably 30 h; the initial viable cell concentration of *Candida utilis* is preferably 1.0-1.5 × 10⁻⁶. 8 CFU / mL, further preferably 1.5 × 10⁻⁶8 CFU / mL.

[0023] This invention also provides a method for preparing agricultural enzymes from solid waste of the aforementioned aquaponics system, comprising the following steps: (1) Bottom sediment was captured from the aquaponics system; (2) Collect dead fish and vegetable waste from the aquaponics system, crush the dead fish into fish paste, and mix the vegetable waste and water in equal proportions and crush them to obtain vegetable waste juice. (3) Mix the bottom mud, fish mud, vegetable juice, carbon source, and compound microbial agent, and ferment to obtain agricultural enzymes from solid waste of aquaponics system.

[0024] In this invention, the fermentation temperature in step (3) is 25~35℃, preferably 30℃; the fermentation time is 25~35d, preferably 30d.

[0025] The present invention also provides the application of agricultural enzymes from the solid waste of the aquaponics system in inhibiting plant pathogens.

[0026] In this invention, the plant pathogens include Alternaria solanacearum and Fusarium oxysporum.

[0027] This invention also provides the application of agricultural enzymes derived from solid waste in aquaponics systems in vegetable cultivation within aquaponics systems.

[0028] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0029] The strains of this invention include Lactobacillus hesitantii. Lactobacillus hilgardii RX (ZL202010939069.6), Candida utilis Candida utilis EMj (see reference: Xie Fengxing, Zhou Ke, Zhang Fengfeng, et al. Mixed fermentation of Candida utilis and Lactobacillus plantarum and its effect on water purification, Food Fermentation and Industry, 2019, 45(6): 185-192.) and Bacillus subtilis Bacillus subtilis K8 (see CN112011486A-A Synchronous Nitrifying and Denitrifying Bacillus subtilis K8 and its Application).

[0030] Example 1

[0031] Preparation of agricultural enzymes

[0032] (1) Collect bottom sediment captured by the microfilter and arc screen in the aquaponics system; Collect dead fish and vegetable waste from the aquaponics system. Crush the dead fish directly and add an equal amount of water to the vegetable waste and crush it. (2) Take 15 g / L brown sugar, 8 g / L yeast extract, 5 g / L sodium acetate, 2 g / L diammonium citrate, 0.02 g / L manganese sulfate, and 2 g / L potassium dihydrogen phosphate and add them to water. Sterilize at 121℃ for 20 min to obtain MRS medium. Inoculate Lactobacillus hessler RX strain into MRS medium at an inoculum of 5 vt% and culture at 35℃ for 24 h to obtain Lactobacillus hessler bacterial suspension. Its initial viable cell concentration is 7.2 × 10⁻⁶. 8 CFU / mL; (3) Take 10 g / L soluble starch, 10 g / L peptone, 3 g / L sodium chloride, 1 g / L calcium carbonate, 1 g / L magnesium sulfate, and 1 g / L dipotassium hydrogen phosphate, add them to water, and sterilize at 121℃ for 20 min to obtain Bacillus GLY medium; inoculate Bacillus strain K8 into the medium at an inoculum of 5 vt%, and culture at 37℃ for 32 h to obtain Bacillus bacterial suspension with an initial viable cell concentration of 1.2 × 10⁻⁶. 9 CFU / mL; (4) YPD medium was prepared by adding 10 g / L yeast extract, 10 g / L peptone, and 20 g / L glucose to water and sterilizing at 115°C for 20 min. The *Candida utilis* EMj strain was inoculated into the YPD medium at a 5 VT inoculum and cultured at 28°C for 30 h to obtain the yeast culture solution with an initial viable cell concentration of 1.5 × 10⁻⁶ cells / mL. 8 CFU / mL; (5) Take the bottom mud, vegetable juice and fish paste and mix them evenly in a ratio of 2:1:1. Add 2.5% carbon source, put it into a plastic container with a one-way valve, add 3% mixed bacterial solution (lactic acid bacteria, yeast and spores mixed in equal proportion), stir evenly, and anaerobic ferment at 30℃ for 30 days to obtain agricultural enzyme.

[0033] Example 2

[0034] Preparation of agricultural enzymes

[0035] (1) Collect bottom sediment captured by the microfilter and arc screen in the aquaponics system; Collect dead fish and vegetable waste from the aquaponics system. Crush the dead fish directly and add an equal amount of water to the vegetable waste and crush it. (2) Take 10 g / L brown sugar, 5 g / L yeast extract, 2 g / L sodium acetate, 1 g / L diammonium citrate, 0.01 g / L manganese sulfate, and 1 g / L potassium dihydrogen phosphate and add them to water. Sterilize at 121℃ for 20 min to obtain MRS medium. Inoculate Lactobacillus hessler RX strain into MRS medium at an inoculum of 5 vt% and culture at 25℃ for 18 h to obtain Lactobacillus hessler bacterial suspension. Its initial viable cell concentration is 5 × 10⁻⁶. 8 CFU / mL; (3) Take 7 g / L soluble starch, 7 g / L peptone, 2 g / L sodium chloride, 0.5 g / L calcium carbonate, 0.5 g / L magnesium sulfate, and 0.5 g / L dipotassium hydrogen phosphate, add them to water, and sterilize at 121℃ for 20 min to obtain Bacillus GLY medium; inoculate Bacillus strain K8 into the medium at an inoculum of 5 vt%, and culture at 25℃ for 18 h to obtain Bacillus bacterial suspension with an initial viable cell concentration of 1.0 × 10⁻⁶. 9 CFU / mL; (4) YPD medium was prepared by adding 7 g / L yeast extract, 15 g / L peptone, and 20 g / L glucose to water and sterilizing at 115°C for 20 min. The *Candida utilis* EMj strain was inoculated into the YPD medium at a 5 VT% inoculum and cultured at 25°C for 24 h to obtain the yeast culture solution with an initial viable cell concentration of 1.0 × 10⁻⁶ cells / mL. 8 CFU / mL; (5) Take the bottom mud, vegetable juice and fish paste and mix them evenly in a ratio of 2:4:1. Add 2.5% carbon source, put it into a plastic container with a one-way valve, add 3% mixed bacterial solution (lactic acid bacteria, yeast and spores mixed in equal proportion), stir evenly, and anaerobic ferment at 25℃ for 30 days to obtain agricultural enzyme.

[0036] Example 3

[0037] Preparation of agricultural enzymes

[0038] (1) Collect bottom sediment captured by the microfilter and arc screen in the aquaponics system; Collect dead fish and vegetable waste from the aquaponics system. Crush the dead fish directly and add an equal amount of water to the vegetable waste and crush it. (2) Take 20 g / L brown sugar, 15 g / L yeast extract, 10 g / L sodium acetate, 5 g / L diammonium citrate, 0.05 g / L manganese sulfate, and 4 g / L potassium dihydrogen phosphate and add them to water. Sterilize at 121℃ for 20 min to obtain MRS medium. Inoculate Lactobacillus hessler RX strain into MRS medium at an inoculum of 5 vt% and culture at 40℃ for 36 h to obtain Lactobacillus hessler bacterial suspension. Its initial viable cell concentration is 7.2 × 10⁻⁶. 8 CFU / mL; (3) Take 15 g / L soluble starch, 25 g / L peptone, 5 g / L sodium chloride, 1.5 g / L calcium carbonate, 1.5 g / L magnesium sulfate, and 1.5 g / L dipotassium hydrogen phosphate, add them to water, and sterilize at 121℃ for 20 min to obtain Bacillus GLY medium; inoculate Bacillus strain K8 into the medium at an inoculum of 5 vt%, and culture at 40℃ for 36 h to obtain Bacillus bacterial suspension with an initial viable cell concentration of 1.0 × 10⁻⁶. 9 CFU / mL; (4) YPD medium was prepared by adding 15 g / L yeast extract, 10 g / L peptone, and 20 g / L glucose to water and sterilizing at 115°C for 20 min. The *Candida utilis* EMj strain was inoculated into the YPD medium at a 5 VT inoculum and cultured at 30°C for 48 h to obtain the yeast culture solution with an initial viable cell concentration of 1.0 × 10⁻⁶ cells / mL. 8 CFU / mL; (5) Take the bottom mud, vegetable juice and fish paste and mix them evenly in a ratio of 4:4:2. Add 2.5% carbon source, put it into a plastic container with a one-way valve, add 3% mixed bacterial solution (lactic acid bacteria, yeast and spores mixed in equal proportion), stir evenly, and anaerobic ferment at 35℃ for 30 days to obtain agricultural enzyme.

[0039] Example 4

[0040] The difference from Example 1 is that the inoculum amount of the mixed bacterial solution is 10%.

[0041] Experimental Example 1

[0042] Determination of indicators of agricultural enzymes

[0043] Effective viable cell count determination: Take 25 mL samples of each enzyme prepared in Examples 1 and 4, add 225 mL of sterile water, place on a shaker at 200 r / min for 30 min, and then serially dilute to obtain concentrations of 10. -3 10 -4 10 -5 The diluent.

[0044] (1) Lactobacillus assay

[0045] Dilute the shake plate using MRS medium and take 10 -3 10 -4 10 -5 1 mL of the diluted sample was incubated at 36°C for 48 h using the pouring method.

[0046] (2) Bacillus assay

[0047] Take 10 -3 10 -4 10 -5 0.1 mL of the diluted sample was plated using LB medium and incubated at 30°C for 48 h.

[0048] (3) Determination of yeast

[0049] Take 10 -3 10 -4 10 -5 0.1 mL of the diluted sample was plated using PDA medium and incubated at 28°C for 48 h.

[0050] (4) Acetic acid bacteria determination

[0051] The culture medium formula is 10g yeast extract, 10g glucose, and 1000mL water (3% anhydrous ethanol; 2g calcium carbonate / bottle added when pouring the plates). Take 10... -3 10 -4 10 -5 Spread 0.1 mL of the diluted sample onto a plate and incubate at 30°C for 72 h.

[0052] (5) Mold assay

[0053] The sample was poured into a 1 mL Erlenmeyer flask using PDA medium and cultured at 28°C for 5 days.

[0054] Take 0.1 mL of each dilution and inoculate it into MRS, LB and PDA media respectively. After culturing at 30℃ for 2 days, determine the effective viable count of each agricultural enzyme.

[0055] Experimental results are shown in Table 1.

[0056] Table 1. Determination Indicators of Agricultural Enzymes

[0057] Table 1 shows that no mold was detected in either the control or the inoculated treatment. The total amount of probiotics in the inoculated treatment was higher than that in the uninoculated treatment, especially in the 10% inoculation treatment, where the total amount of probiotics was 1.5 times that of the control, and the number of acetic acid bacteria was more than 4 times that of the control. Acetic acid bacteria can decompose large organic molecules into small organic acids, amino acids, etc., and secrete some antibacterial substances during reproduction, which can effectively inhibit or repel the reproduction of some soil-borne pathogens, thereby reducing the occurrence of crop diseases and reducing the amount of pesticides used.

[0058] Experiment Example 2

[0059] The antibacterial effect of agricultural enzymes on plant pathogens

[0060] (1) The pathogen of early blight of tomato, Alternaria solanacearum ( Alternaria solani ) and the pathogen of cucumber wilt, Fusarium oxysporum ( Fusarium oxysporum (The strain was obtained from the Agricultural Microbial Germplasm Resource Bank of Tianjin Academy of Agricultural Sciences) The fungal cake was made by punching holes and a 7 mm diameter, vigorous pathogenic fungal cake was inoculated in the center of a PDA plate with the mycelial side facing down.

[0061] (2) At a distance of 2 cm from the pathogen, 10-fold diluted agricultural enzyme solution (Oxford cup) was symmetrically inoculated, with the culture dish without enzyme inoculation as the control. Each treatment was repeated in 3 replicates and incubated in the dark at 25℃.

[0062] (3) When the diameter of the pathogen in the control group reaches 3 / 4 of the diameter of the culture dish, the diameter of the pathogen colony is measured by the cross-cross method and the inhibition rate is calculated.

[0063] Inhibition rate / % = (Coronavirus diameter of control group - Coronavirus diameter of treatment group) / (Coronavirus diameter of control group - 7) × 100.

[0064] Experimental results: such as Figure 1 and Figure 2 As shown, 10-fold dilutions of different treated enzymes showed inhibition rates of 55.9%–58.8% against early blight of tomatoes and 58.1%–61.3% against wilt of cucumbers. This indicates that the enzymes fermented from bottom mud, dead fish, and vegetable waste have a certain inhibitory effect on plant pathogens and can be used for the prevention and control of diseases in planting units.

[0065] As can be seen from the above embodiments and experimental examples, the agricultural enzyme of the present invention uses bottom mud, dead fish and vegetable waste as fermentation raw materials, has high nutrient content, and has a high effective live bacteria concentration of beneficial bacteria after fermentation. It can not only provide nutrients for plant growth and promote plant growth, but also have a certain inhibitory effect on plant pathogens in the system.

[0066] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An agricultural enzyme for solid waste from an aquaponics system, characterized in that, The mixture includes bottom mud, dead fish, and vegetable waste, with a mass ratio of 2~4:2~4:1~2. The bottom mud, dead fish, and vegetable waste are all produced by the aquaponics system.

2. The aquaponics system solid waste agricultural enzyme according to claim 1, characterized in that, It also includes 2-3% carbon source of the total mass of bottom mud, dead fish, and vegetable waste, and 3-10% compound microbial agent of the total mass of bottom mud, dead fish, and vegetable waste.

3. The aquaponics system solid waste agricultural enzyme according to claim 2, characterized in that, The compound microbial agent includes Lactobacillus hessei, Bacillus subtilis, and Candida utilis, with the mass ratio of Lactobacillus hessei, Bacillus subtilis, and Candida utilis being 1:1:

1.

4. The aquaponics system solid waste agricultural enzyme according to claim 3, characterized in that, The initial viable concentration of *Lactobacillus hesitantus* was 5.0–7.2 × 10⁻⁶. 8 The initial viable concentration of Bacillus subtilis was 1.0~1.2×10 CFU / mL. 9 The initial viable concentration of *C. utilis* was 1.0–1.5 × 10⁻⁶ CFU / mL. 8 CFU / mL.

5. The aquaponics system solid waste agricultural enzyme according to claim 2, characterized in that, The carbon source includes molasses.

6. The method for preparing agricultural enzymes from solid waste of aquaponics systems according to any one of claims 1 to 5, characterized in that, Includes the following steps: (1) Bottom sediment was captured from the aquaponics system; (2) Collect dead fish and vegetable waste from the aquaponics system, crush the dead fish into fish paste, and mix the vegetable waste and water in equal proportions and crush them to obtain vegetable waste juice. (3) Mix the bottom mud, fish mud, vegetable juice, carbon source, and compound microbial agent, and ferment to obtain agricultural enzymes from solid waste of aquaponics system.

7. The preparation method according to claim 6, characterized in that, The fermentation temperature in step (3) is 25~35℃, and the fermentation time is 25~35 days.

8. The application of agricultural enzymes from solid waste of aquaponics systems according to any one of claims 1 to 5 in inhibiting plant pathogens.

9. The application according to claim 8, characterized in that, The plant pathogens include Alternaria solanacearum and Fusarium oxysporum.

10. The application of the aquaponics system solid waste agricultural enzyme as described in any one of claims 1 to 5 in the vegetable cultivation of aquaponics systems.

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