Cultivated bamboo bird's nest and application thereof

Abstract

This invention discloses a cultivable bamboo bird's nest and its applications, belonging to the field of biotechnology. The cultivable bamboo bird's nest of this invention is named Green Coral (accession number BBN1), and was deposited on September 10, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO.42097. This invention collects wild bamboo bird's nests from different origins and environments, and after multiple rounds of screening, finally obtained the cultivable bamboo bird's nest strain of this invention. Compared with wild bamboo bird's nests, the bamboo bird's nest strain of this invention can be stably cultivated artificially. The fruiting bodies are generally light green in color, gradually deepening to dark green with prolonged storage after harvesting. The mycelium is crystal clear, pure, and free of impurities, and its growth temperature range is wider than that of wild bamboo bird's nests. The cultivable bamboo bird's nest strain of this invention fills the gap in the domestic and international markets that have long relied on wild harvesting, and has broad market prospects.

Description

[0001] This application is a divisional application of patent application No. 202511655258.X, filed on November 12, 2025. Technical Field

[0002] This invention relates to the field of biotechnology, specifically to a cultivable bamboo bird's nest and its applications. Background Technology

[0003] Bamboo bird's nest, scientifically known as *Scorias spongiosa*, also called bamboo fungus, bamboo flower, or bamboo mushroom, is a valuable type of fungal green food. It originates from the secretions left on bamboo by the bamboo aphid after it feeds on the sap of young bamboo. Under suitable temperature and humidity, these secretions are utilized by microorganisms, forming a natural fungal-microbial symbiosis. Because its appearance and taste are similar to bird's nest, with a fresh, smooth texture, it was named bamboo bird's nest. Bamboo bird's nest contains various essential and non-essential amino acids, proteins, vitamins, and polysaccharides, among other active substances. It possesses antibacterial, anti-inflammatory, and antioxidant properties, making it a novel edible fungal resource with significant economic value and development potential. However, current research indicates that bamboo bird's nest cannot form large fruiting bodies, and its market availability relies entirely on wild collection, making large-scale artificial production difficult. Therefore, it is essential to develop a method for artificially cultivating bamboo bird's nest. Summary of the Invention

[0004] In order to overcome the above-mentioned shortcomings of the existing technology, the purpose of this invention is to provide a cultivable bamboo bird's nest and its application, so as to solve the problem that existing bamboo bird's nests cannot form large fruiting bodies and are difficult to achieve artificial large-scale production.

[0005] The technical solution of the present invention to solve the above-mentioned technical problems is as follows: a cultivable bamboo bird's nest is provided, which is named Green Coral (number BBN1), and was deposited at the China General Microbiological Culture Collection Center on September 10, 2025, with the accession number CGMCC NO.42097, and classified as Scorias spongiosa.

[0006] This invention provides the application of the above-mentioned cultivable bamboo bird's nest in the preparation of food or health products.

[0007] The present invention provides a food product, including the above-mentioned cultivable bamboo bird's nest.

[0008] This invention provides a health product, including the above-mentioned cultivable bamboo bird's nest.

[0009] This invention provides an application of the above-mentioned cultivable bamboo bird's nest in bamboo bird's nest agricultural cultivation.

[0010] This invention also provides a method for cultivating bamboo bird's nest in agriculture, including the following steps: inoculating the fruiting bodies of the cultivable bamboo bird's nest strain onto a culture rack, controlling the temperature at 20-23℃, the air humidity at 90-95%, the carbon dioxide concentration at less than 1000ppm, cultivating in the dark, spraying nutrient solution every 3 hours, and cultivating for 15-20 days.

[0011] Furthermore, the nutrient solution includes the following components: glucose, honey, sugar, and water.

[0012] Furthermore, the mass ratio of glucose, honey, white sugar, and water is 15-20:1-5:1-5:70-75.

[0013] Furthermore, the mass ratio of glucose, honey, white sugar, and water is 20:2:3:75.

[0014] This invention offers the following advantages: It collects wild bamboo bird's nests from different origins and environments, and obtains single-spore bamboo bird's nest strains capable of forming fruiting bodies through spore isolation and culture. After multiple rounds of screening, the cultivable bamboo bird's nest strain of this invention is finally obtained. Compared to wild bamboo bird's nests, the bamboo bird's nest strain of this invention can be stably cultivated artificially. The fruiting bodies are initially light green, gradually turning dark green with increasing storage time. The mycelium is crystal clear, pure, and free of impurities, and its growth temperature range is wider than that of wild bamboo bird's nests, ranging from 10-23℃. This cultivable bamboo bird's nest strain fills the gap in the domestic and international markets that have long relied on wild harvesting, giving the cultivable bamboo bird's nest of this invention a broad market prospect. Attached Figure Description

[0015] Figure 1 It is a strain of wild bamboo bird's nest;

[0016] Figure 2 This invention relates to the artificially cultivated bamboo bird's nest strain "Green Coral". Detailed Implementation

[0017] The examples given below are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified, conditions in the examples are performed under standard conditions or as recommended by the manufacturer. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0018] Example

[0019] A method for isolating and artificially cultivating cultivable bamboo bird's nests, specifically including the following steps:

[0020] (1) Separation of cultivable bamboo bird's nest

[0021] 1. Collect bamboo bird's nests from the Shunan Bamboo Sea in Sichuan and Guizhou;

[0022] 2. Preparation of PDA plate culture medium: Weigh 200g of peeled potatoes and cut them into strips. Put them in a pot, add 1L of deionized water, and then cook the potato strips until soft. Filter the medium and pour the filtrate into a beaker. Add deionized water to make up the volume of the filtrate to 1L. Add 20g of glucose and 18g of agar powder, and heat until completely dissolved. While hot, pour the culture medium into a petri dish (15mL of culture medium) and let it cool and solidify.

[0023] 3. Under aseptic conditions, a small piece of mycelial tip tissue was cut from wild bamboo bird's nests collected from different regions using a sterile blade and placed in an appropriate amount of sterile water to obtain conidial suspensions from wild bamboo bird's nests from different regions.

[0024] 4. Spread these spore suspensions onto the PDA plate medium prepared in step 2, and then incubate them in a constant temperature incubator at 25℃ for 3 days. Observe the germination status of the bamboo bird's nest single spore colonies under a stereomicroscope. Select the colonies with good growth and transfer them to the PDA plate medium prepared in step 2 using a sterile inoculation spoon. Continue to incubate them in a constant temperature incubator at 25℃ for 5 days.

[0025] 5. Using a sterile tissue separation blade and inoculation hook, cut a portion of the mycelial tissue from the combination selected in step 4, inoculate it onto the PDA plate medium prepared in step 2 for subculture again, and then place it in a constant temperature incubator at 25℃ for 5 days to obtain a bamboo bird's nest strain with stable genetic traits.

[0026] (2) Artificial cultivation methods for cultivating bamboo bird's nest

[0027] The young fruiting bodies of the bamboo bird's nest strain obtained in step (1) were placed on a raised arc-shaped plastic culture rack. The ambient temperature was controlled at 21°C, the air humidity at 90%, the carbon dioxide concentration at 500 ppm, and the environment was kept away from light. A special nutrient solution (the culture solution formula is: 20 wt% glucose, 2 wt% honey, 3 wt% white sugar and 75 wt% water) was sprayed every 3 hours and cultured for 15-20 days.

[0028] The strains of wild bamboo bird's nest and the bamboo bird's nest artificially cultivated according to this invention are described in [the following text is missing]. Figure 1-2This invention, by comparing the growth rate, volume, and color of fruiting bodies, screened out a bamboo bird's nest strain, "Green Coral" (BBN1), which exhibits excellent comprehensive traits, including rapid growth, large fruiting body volume, and vibrant color, and possesses stable genetic characteristics. Compared to wild bamboo bird's nests, which grow only once a year depending on the season and are characterized by branch diameters of approximately 0.5 mm and variable sizes (single cluster length and width ranging from 1 cm to 10 cm), and are often covered with dead branches, decaying leaves, and aphids, this invention's bamboo bird's nest strain can achieve approximately 20 growth cycles per year through artificial cultivation. The branch diameter can reach approximately 1.0 mm, with uniform size (single cluster length and width reaching approximately 15 cm), and the fruiting bodies are crystal clear, pure, and free of impurities, resulting in superior quality. By optimizing its cultivation conditions, the artificial large-scale production and cultivation of the bamboo bird's nest strain "Green Coral" has been successfully achieved, giving it broad market prospects.

[0029] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for cultivating bamboo edible bird's nest agriculture, characterized in that, The process includes the following steps: inoculate the fruiting bodies of the cultivable bamboo bird's nest strain onto a culture rack, control the temperature at 20-23℃, the air humidity at 90-95%, the carbon dioxide concentration at less than 1000ppm, culture in the dark, spray nutrient solution every 3 hours, and culture for 15-20 days.

2. The cultivable bamboo bird's nest agricultural planting method according to claim 1, characterized in that, The cultivable bamboo bird's nest, named Green Coral, was deposited at the China General Microbiological Culture Collection Center on September 10, 2025, with the accession number CGMCC NO.42097.

3. The method according to any one of claims 1-2, wherein the method is characterized in that, The nutrient solution comprises the following components: glucose, honey, sugar, and water.

4. The method according to any one of claims 1-3, wherein the method is characterized in that, The mass ratio of glucose, honey, white sugar and water is 15-20:1-5:1-5:70-75.

5. The method according to any one of claims 1-4, wherein the method is characterized in that, The mass ratio of glucose, honey, white sugar and water is 20:2:3:75.

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