The application of enzymatic hydrolysis cottonseed meal in preparation of low-protein daily ration, promotion of intestinal development of animals, improvement of production performance of animals and improvement of carcass quality

By preparing low-protein diets through enzymatic hydrolysis of cottonseed meal and using compound protease to hydrolyze cottonseed meal, the problems of amino acid imbalance and anti-nutritional factor limitation in cottonseed meal in animal feed are solved, improving animal intestinal health and production performance, and reducing feeding costs.

CN122250561APending Publication Date: 2026-06-23XINJIANG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XINJIANG AGRI UNIV
Filing Date
2026-05-14
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

The current application of cottonseed meal in animal feed is limited by amino acid imbalance and anti-nutritional factors, resulting in low utilization efficiency in low-protein diets, and the reduction of protein levels may be accompanied by a decline in production performance.

Method used

Enzymatically hydrolyzed cottonseed meal was prepared by hydrolyzing cottonseed meal using a complex protease. By hydrolyzing protein peptide bonds, large protein molecules were degraded into small peptides and free amino acids. The complex protease obtained by liquid fermentation of Bacillus subtilis had an enzyme activity of 50,000–80,000 U/g. The hydrolysis time was 24–72 h and the temperature was 30–40 °C. Enzymatically hydrolyzed cottonseed meal can replace part or all of the cottonseed meal to prepare low-protein diets.

Benefits of technology

It increases the content of small peptides and free amino acids in enzymatically hydrolyzed cottonseed meal, improves animal gut health, enhances production performance and carcass quality, reduces feeding costs, and achieves efficient utilization of low-protein diets.

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Abstract

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.
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