A protein-based hydrogel in-situ crosslinking agent, in-situ crosslinked hydrogel and application thereof

The protein-based composite hydrogel formed by crosslinking mercapto-polyethylene glycol and metal ions with proteins solves the problems of biocompatibility and gelation time of existing sealing materials, realizes rapid sealing and reversible desealing, and expands the application scenarios.

CN122272874APending Publication Date: 2026-06-26SHANGHAI RUINING BIOTECH CO LTD
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI RUINING BIOTECH CO LTD
Filing Date
2026-04-01
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing occlusion materials have problems in clinical applications, such as poor biocompatibility, excessively long gelation time, insufficient mechanical strength, excessively rapid or irreversible degradation, and high swelling rate, which cannot meet the clinical needs of minimally invasive occlusion and rapid de-occlusion.

Method used

Thiol-modified polyethylene glycol (PEGSH) and metal ions (such as Cu2+) are used to crosslink proteins with proteins through metal coordination to form protein-based composite hydrogels, achieving rapid gelation, self-healing, low swelling rate, and reversible gelation.

Benefits of technology

It achieves rapid gelation (gelation within 3 seconds), strong self-healing ability, low swelling rate, good elasticity, and reversible gelation, expanding the application scenarios of hydrogels in body cavity sealing materials.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122272874A_ABST
    Figure CN122272874A_ABST
Patent Text Reader

Abstract

This invention belongs to the field of biomaterials and discloses a protein-based hydrogel in-situ crosslinking agent. The in-situ crosslinking agent is characterized by being composed of a first precursor solution and a second precursor solution. The first precursor solution is prepared by dissolving thiolated polyethylene glycol (PEGSH) and protein in a buffer solution and adjusting the pH to 7.2 with NaOH. The second precursor solution is prepared by dissolving CuSO4 in deionized water. This protein-based hydrogel in-situ crosslinking agent possesses injectability, rapid gelation properties, and the hydrogel formed after crosslinking exhibits strong self-healing ability, low swelling ratio, good elasticity, and is not easily crushed. Furthermore, it can rapidly disintegrate under the action of a degelating agent.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Injectable protein / polyethylene glycol-based hydrogel material and preparation method and application thereof

    CN110193091A

  • A method for constructing non-denatured protein hydrogels using molecular self-assembly technology

    CN114315994B