Method for producing amino acid-containing water-soluble fertilizer by using sugar cane molasses and organic cake fertilizer

Abstract

This invention discloses a method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake. The method involves first establishing an equation relating the composting time of organic oilseed cake to influencing factors, then performing material mixing, enzymatic hydrolysis, centrifugation, filtration, enzyme inactivation, preservation, and adjustment of the effective component content to obtain the amino acid-containing water-soluble fertilizer of this invention. This invention can rapidly compost organic oilseed cake within 12 hours, resulting in a stable fertilizer product with a storage period of ≥3 months. It will not undergo further enzymatic hydrolysis to produce odors or secondary metabolites, and its solubility is ≥95%, which is beneficial for the promotion of fertigation technology. The fertilizer has a high amino acid content, and the nutrient content can be adjusted as needed, significantly improving crop yield and quality after application.

Description

Technical Field

[0001] This invention relates to the field of water-soluble fertilizer production technology, specifically a method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic cake fertilizer. Background Technology

[0002] Amino acid-containing water-soluble fertilizers, as a new type of functional fertilizer, can be rapidly absorbed by crops and have the characteristics of increasing yield, improving fruit quality, enhancing stress resistance, improving soil, and increasing fertilizer utilization. They play a positive role in solving problems such as soil quality decline, environmental pollution, and pesticide residues caused by the excessive use of chemical fertilizers and pesticides in agricultural production. With the rapid development of agriculture in my country, the expansion of intensive land use, and the continuous advancement of integrated water and fertilizer management, agriculture's demand for low-input, high-output, environmentally friendly fertilizers that also improve soil quality will usher in a new era of development for amino acid water-soluble fertilizers.

[0003] In the production of amino acid-containing water-soluble fertilizer from organic cake fertilizer, the enzymatic cleavage reaction of protein-degrading enzymes is a crucial step. Traditional production processes often omit steps such as material crushing, heating, aeration, stirring, centrifugation, filtration, enzyme inactivation, preservation, and adjustment of the content of effective ingredients, resulting in problems such as long composting time, low amino acid conversion efficiency, poor water solubility of fertilizer products, and short shelf life. Summary of the Invention

[0004] The purpose of this invention is to address the problems in the existing technology of producing amino acid-containing water-soluble fertilizer from organic cake fertilizer, such as long composting time, low amino acid conversion efficiency, poor water solubility of fertilizer products, and short storage period, by providing a method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic cake fertilizer.

[0005] 1. To achieve the above objectives, the present invention provides the following technical solution: a method for producing water-soluble fertilizer containing amino acids using sugarcane molasses and organic oilseed cake, comprising the following steps:

[0006] Step 1: Establish the relationship equation between the composting time of organic cake fertilizer and influencing factors;

[0007] Step 2, material mixing: Mix sugarcane molasses, organic oilseed cake, fermentation agent, and water;

[0008] Step 3, enzymatic hydrolysis: Enzymatic hydrolysis is performed under conditions of heating, stirring, and aeration;

[0009] Step 4, centrifugation: Centrifuge using a centrifuge;

[0010] Step 5, filtration: Filter using a 100-120 mesh sieve;

[0011] Step 6, Enzyme Inactivation: Inactivate enzymes by heating;

[0012] Step 7, corrosion prevention: Add 20% magnesium nitrate for corrosion prevention;

[0013] Step 8, Adjust the content of effective ingredients: Adjust the content of amino acids, nitrogen, phosphorus, potassium and trace elements to form a water-soluble fertilizer product containing amino acids.

[0014] Furthermore, in step one, the relationship equations between the composting time of organic cake fertilizer and influencing factors are established as follows: Y = -0.1876X1 + 34.167, Y = -0.9667X2 + 51.333, Y = -3.0333X3 + 23.9, Y = -1.7471X4 + 15.928, where Y is the composting time of organic cake fertilizer (d), X1 is the mesh size of the sieve through which the organic cake fertilizer is crushed (mesh), X2 is the enzymatic hydrolysis reaction temperature of the mixture (°C), X3 is the enzymatic hydrolysis stirring time of the mixture (h / d), and X4 is the amount of air introduced into the mixture during enzymatic hydrolysis (m³). 3 / h).

[0015] Furthermore, steps two and three are determined based on the relationship equation between the composting time of organic cake fertilizer and influencing factors. In step two, the sugar content of sugarcane molasses is ≥40%, the total nutrients are ≥4%, the organic cake fertilizer is crushed to 100-200 mesh, the solid-liquid ratio of organic cake fertilizer to water is 1:5-10, the amount of sugarcane molasses added accounts for 2%-5% of the total weight, and the fermentation agent is rich in protein decomposing enzymes with protein decomposing enzyme activity ≥15U / g or ml, and the amount added is 0.1%-0.3%.

[0016] Furthermore, in step three, the heating temperature during enzymatic hydrolysis is 40-60℃, the stirrer power is 7-15 kWh, the stirring rate is 30-60 rpm, the stirring time is 3-9 h / d, and the aerator is an air aerator with a power of 300-450 kWh and an aeration volume of 0.5-10 m³. 3 / h, fermentation time is 0.5-7d.

[0017] Furthermore, in step four, the centrifuge has a power of 4-6 kilowatts and a rotation speed of 1000-1400 revolutions per minute.

[0018] Furthermore, in step six, the enzyme inactivation temperature is 70-90℃, and the enzyme inactivation time is 10-30 minutes.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] This invention first establishes a relationship equation between the composting time of organic cake fertilizer and factors such as the degree of crushing of organic cake fertilizer, the enzymatic hydrolysis reaction temperature of the mixture, the enzymatic hydrolysis stirring time, and the amount of air introduced during enzymatic hydrolysis. Then, it adjusts the crushing, heating, stirring, and aeration of materials to improve the enzymatic hydrolysis efficiency of organic cake fertilizer. Next, it improves the solubility of water-soluble fertilizer by centrifugation and filtration. Finally, it improves the storage period of water-soluble fertilizer by inactivating enzymes and preventing decay. Detailed Implementation

[0021] The technical solution of the present invention will be further described in detail below with reference to specific embodiments, but the present invention is not limited thereto.

[0022] Example 1

[0023] Sugarcane molasses with a total sugar content of 42% and a total nutrient content of 4.2% and peanut bran ground to 200 mesh were used for enzymatic hydrolysis to produce an amino acid-containing water-soluble fertilizer. Water was added, the solid-liquid ratio was 1:7, and the sugarcane molasses accounted for 4% of the total weight. An endonuclease (protein-degrading enzyme activity of 15.5 U / g) was added at 0.3% of the total weight of the reaction solution. The enzymatic hydrolysis reaction was carried out at a heating temperature of 55℃, a stirring time of 9 hours / day, and an aeration rate of 10 m³ / min. 3 The enzyme hydrolysis time is 0.5 days, followed by centrifugation and filtration at 1400 rpm using a 120-mesh sieve. Next, enzyme inactivation and preservation are performed at 90℃ for 30 minutes. 20% magnesium nitrate is added for preservation. Finally, the amino acid and nutrient content is adjusted to form an amino acid-containing water-soluble fertilizer product with an amino acid content of 10% and a total nutrient content of 5%. This product is diluted 10-50 times for drip irrigation or drenching of crops.

[0024] Example 2:

[0025] Sugarcane molasses with a total sugar content of 40% and a total nutrient content of 4.0% and peanut bran ground to 150 mesh were used for enzymatic hydrolysis to produce an amino acid-containing water-soluble fertilizer. Water was added, with a solid-liquid ratio of 1:10. Sugarcane molasses accounted for 3% of the total weight. An endonuclease (protein-degrading enzyme activity of 15.8 U / g) was added at 0.3% of the total weight of the reaction solution. The enzymatic hydrolysis reaction was carried out at 50℃, with stirring time of 6 hours / day and an aeration rate of 2.5 m³ / h. 3 The enzyme hydrolysis time is 3 days, followed by centrifugation and filtration at 1400 r / min using a 120-mesh sieve. Next, enzyme inactivation and preservation are performed at 90℃ for 30 minutes. 20% magnesium nitrate is added for preservation. Finally, the amino acid and nutrient content is adjusted to form an amino acid-containing water-soluble fertilizer product with an amino acid content of 10% and a total nutrient content of 3%. This product is diluted 6-30 times for drip irrigation or drenching of crops.

[0026] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

Claims

1. A method for producing water-soluble fertilizer containing amino acids using sugarcane molasses and organic oilseed cake, characterized in that: Includes the following steps: Step 1: Establish the relationship equation between the composting time of organic cake fertilizer and influencing factors; Step 2, material mixing: Mix sugarcane molasses, organic oilseed cake, fermentation agent, and water; Step 3, enzymatic hydrolysis: Enzymatic hydrolysis is performed under conditions of heating, stirring, and aeration; Step 4, centrifugation: Centrifuge using a centrifuge; Step 5, filtration: Filter using a 100-120 mesh sieve; Step 6, Enzyme Inactivation: Inactivate enzymes by heating; Step 7, corrosion prevention: Add 20% magnesium nitrate for corrosion prevention; Step 8, Adjust the content of effective ingredients: Adjust the content of amino acids, nitrogen, phosphorus, potassium and trace elements to form a water-soluble fertilizer product containing amino acids.

2. The method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake according to claim 1, characterized in that: In step one, the relationship equations between the composting time of organic cake fertilizer and influencing factors are established as follows: Y = -0.1876X1 + 34.167, Y = -0.9667X2 + 51.333, Y = -3.0333X3 + 23.9, Y = -1.7471X4 + 15.928, where Y is the composting time of organic cake fertilizer (d), X1 is the mesh size of the sieve through which the organic cake fertilizer is crushed (mesh), X2 is the enzymatic hydrolysis reaction temperature of the mixture (°C), X3 is the enzymatic hydrolysis stirring time of the mixture (h / d), and X4 is the amount of air introduced into the mixture during enzymatic hydrolysis (m³). 3 / h).

3. The method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake according to claim 1, characterized in that: Steps two and three are determined based on the relationship equation between the composting time of organic oilseed cake and influencing factors. In step two, the sugar content of sugarcane molasses is ≥40%, the total nutrients are ≥4%, the organic oilseed cake is crushed to 100-200 mesh, the solid-liquid ratio of organic oilseed cake to water is 1:5-10, the amount of sugarcane molasses added accounts for 2%-5% of the total weight, and the fermentation agent is rich in protein-degrading enzymes with protein-degrading enzyme activity ≥15U / g or ml, and the amount added is 0.1%-0.3%.

4. The method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake according to claim 1, characterized in that: In step three, the enzymatic hydrolysis is performed at a heating temperature of 40-60℃, with a mixer power of 7-15 kW, a stirring speed of 30-60 rpm, and a stirring time of 3-9 h / d. An air aerator with a power of 300-450 kW and an aeration volume of 0.5-10 m³ is used. 3 / h, fermentation time is 0.5-7d.

5. The method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake according to claim 1, characterized in that: In step four, the centrifuge power is 4-6 kilowatts and the rotation speed is 1000-1400 revolutions per minute.

6. The method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake according to claim 1, characterized in that: In step six, the enzyme inactivation temperature is 70-90℃, and the enzyme inactivation time is 10-30 minutes.