Vibrio volutius bacteriophage with high temperature resistance and screening method and application thereof

By using instantaneous high temperature and rapid cooling treatment to screen for thermoresistant Aeromonas verrucose phages, the problems of long screening cycles and unstable heat resistance in existing technologies have been solved. This method enables the rapid acquisition of thermoresistant phages and reduces production costs, making it suitable for disease prevention and control in aquaculture.

CN122278779APending Publication Date: 2026-06-26QINGDAO RUNDA BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO RUNDA BIOTECH
Filing Date
2026-03-10
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing technologies lack a rapid, chemical-protective method that can stably screen for thermostable Aeromonas versicolor phages with excellent genetic traits. Traditional methods are time-consuming and have unstable heat resistance.

Method used

Thermostable thermoresistant Aeromonas verrucosa phages were screened by instantaneous high temperature and rapid cooling treatment. The phage suspension was treated with high temperature-cooling cycle treatment, and the process was repeated multiple times to obtain stable thermoresistant phages.

Benefits of technology

It achieves stable, heat-resistant bacteriophages within 5 days, reducing production costs by 30%-50%, while maintaining excellent heat resistance and broad-spectrum lytic activity, making it suitable for tropical water bodies and feed processing environments.

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Abstract

This invention relates to the field of microbial technology, specifically disclosing a thermoresistant Aeromonas verrucosa phage, its screening method, and its applications. The phage is deposited under CGMCC No. 47009. This phage belongs to the Myocaudidae family and exhibits significant heat resistance, maintaining a titer of 10 even after treatment at 70°C for 60 minutes. ^ 8 This bacteriophage exhibits a PFU / mL concentration above [amount missing] and can withstand the high temperatures of feed pelleting. It displays broad-spectrum lytic activity against *Aeromonas vesiculosus* and can cross-lyze *Aeromonas guinea pig* and *Aeromonas hydrophila*. This invention also provides a rapid screening method for this heat-resistant bacteriophage. Through a cycle of instantaneous heat treatment at 70°C followed by rapid cooling in an ice bath, genetically stable bacteriophages can be obtained within 5 days, solving the problems of long cycles and poor stability associated with traditional acclimatization methods. The bacteriophage and composition of this invention can be used to prepare veterinary drugs and disinfectants for the prevention and treatment of aquatic aeromonas diseases, showing broad application prospects.
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