A tool and method for determining the optical magnification of an optical microscope

By drawing graphic blocks on a background panel, a simple standard for optical microscope magnification is provided, utilizing the resolution of the human retina. This solves the problem of the lack of a unified standard for optical microscopes and enables performance comparison and removal of electronic magnification interference.

CN122282274APending Publication Date: 2026-06-26SHANGHAI LINGHU MEDICAL EQUIPMENT CO LTD
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Patent Information

Application Number
CN202610390962.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-27
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

The lack of a unified standard for determining the optical magnification of existing optical microscopes makes it difficult for users to compare the performance of different microscopes, especially when using electronic magnification technology, where there is a lack of a unified calculation standard.

Method used

A tool that combines graphic blocks drawn on a background is used to set the magnification of an optical microscope by visually recognizing changes in the sharpness of the graphic blocks, taking advantage of the human retinal resolution of 0.10-0.20mm. This provides a simple standard for optical magnification.

Benefits of technology

It achieves a unified determination of the optical magnification of optical microscopes, avoids software calculation correction, can effectively distinguish the performance of different optical microscopes, and is applicable to optical-electron composite microscopes, eliminating electronic magnification interference.

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Abstract

This invention relates to the field of medical device technology, and more particularly to a tool and method for determining the optical magnification of an optical microscope. The tool includes several columns of graphic blocks drawn on a background plate. Each column of the graphic block combination includes four graphic blocks of equal size. The distance between adjacent graphic blocks is equal. The shapes and sizes of the graphics drawn on the graphic blocks in the same column are the same, but their orientations are different. The sizes of the graphics drawn on adjacent columns decrease by a factor of 1. This invention provides a new, simplified standard for optical magnification, using a method similar to a human vision chart. It is the first to utilize the human retina's resolving limit of 0.10-0.20 mm, representing a cross-disciplinary research achievement between medicine and optics. It solves the technical problem of the lack of a unified standard for determining optical magnification in the market. It can determine the performance differences between different optical magnifying glasses and different optical microscopes.
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Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a tool and method for determining the optical magnification of an optical microscope. Background Technology

[0002] Optical magnification is the magnification capability achieved solely through the physical imaging characteristics of optical lenses (such as lenses, objectives, and eyepieces). It is the size ratio between the image formed on the imaging plane after light passes through the optical system and the actual object. The entire process does not involve any electronic magnification methods (such as digital zoom) or post-processing operations. Its calculation formula is: Optical magnification = Image height / Object height = Image distance / Object distance. In short, optical magnification is the ratio of the size of the image of the object on the imaging plane to the actual size of the object.

[0003] An optical stereo microscope determines the optical magnification of an object by passing it through an objective lens and an eyepiece. The optical magnification is calculated as: objective lens magnification × eyepiece magnification. For example, if the objective lens magnification is 10X and the eyepiece magnification is 4X, the optical magnification of this microscope is 10X × 4X = 40X.

[0004] An optical surgical microscope determines the optical magnification by the distance between the objective lens, eyepiece, zoom lens, and working distance. The optical magnification is calculated as follows: Optical magnification = Focal length of the microscope tube × Eyepiece magnification × Zoom factor of the zoom lens / Focal length of the objective lens. For example, if the focal length of the microscope tube is 170mm, the focal length of the eyepiece is 12.5X, the zoom factor of the zoom lens is 2.4, and the focal length of the objective lens is 200mm, then the optical magnification of this microscope is 170 × 12.5 × 2.4 / 200 = 25.5X.

[0005] With advancements in technology and sophisticated computer software algorithms, optical microscopes now incorporate electronic processing steps, further amplifying the image beyond optical magnification. However, there is no unified standard for calculating electronic magnification. Currently, optical microscopes are manufactured using different objectives, eyepieces, and zoom lenses, each calculating its own magnification, resulting in a lack of standardized criteria for judgment. Users also cannot readily determine the quality of different microscopes. For example, if two microscopes both calculate a magnification of 25.5X, a difference in the size of the object being clearly visible may exist; the smaller the object, the better the microscope's performance. Medical data indicates that the human retina can only resolve objects up to 0.10-0.20 mm in size, meaning the smallest object the human eye can perceive is within this range. Summary of the Invention

[0006] The technical problem to be solved by this invention is to utilize the limit size of the human retina to distinguish objects (0.10-0.20 mm) and apply it to the standard of optical magnification, thereby forming an interdisciplinary research result between medicine and optics, effectively avoiding software calculation correction, etc., and establishing a simple standard for determining the optical magnification of an optical microscope.

[0007] To solve the above-mentioned technical problems, the technical solution of the present invention is: a tool for determining the optical magnification of an optical microscope, comprising several columns of graphic block combinations drawn on a background plate, each column of the graphic block combination comprising four graphic blocks, the graphic blocks being of equal size, the spacing between two adjacent graphic blocks being equal, the shapes and sizes of the graphics drawn on the graphic blocks in the same column being the same but the orientations of the graphics being different, and the sizes of the graphics drawn on the graphic blocks in two adjacent columns decreasing by a factor of 1.

[0008] As a preferred technical solution, the background plate is a transparent plate, the graphic block is a black square block, and the graphic is a transparent herringbone frame with a square opening, the side length of which is equal to the line width of the transparent herringbone frame.

[0009] As a preferred technical solution, the square opening is located in the middle of one of the sides of the transparent loop frame.

[0010] As a preferred technical solution, the size of the graphic view block is 1.200mm*1.200mm, and the distance from each side of the transparent loop frame to the corresponding side on the graphic view block is equal.

[0011] As a preferred technical solution, when the line width of the transparent loop frame is defined as 0.200mm, it is considered to be magnified by 1x. Then, when the line width of the transparent loop frame is 0.100mm, 0.050mm, 0.040mm, 0.020mm, and 0.002mm, the corresponding magnification factors are 2x, 4x, 5x, 10x, and 100x, respectively.

[0012] As a preferred technical solution, when the line width of the transparent loop frame is defined as 0.100mm, it is considered to be magnified by 1x. Then, when the line width of the transparent loop frame is 0.050mm, 0.025mm, 0.020mm, 0.010mm, and 0.001mm, the corresponding magnification factors are 2x, 4x, 5x, 10x, and 100x, respectively.

[0013] As a preferred technical solution, the graphic block combination has 10 columns. The side length of the square opening on the graphic block in the first column is 0.200mm. The side lengths of the square openings on the graphic blocks in the second to tenth columns are 0.100mm, 0.067mm, 0.050mm, 0.040mm, 0.033mm, 0.029mm, 0.025mm, 0.022mm, and 0.020mm, respectively.

[0014] The present invention also provides a method for determining the optical magnification of an optical microscope. Using the above-mentioned tool for determining the optical magnification of an optical microscope, when the side length of the square opening is 0.200mm, the direction of the square opening can be clearly seen by the human eye, and the magnification is defined as 1X at this time.

[0015] When the side length of the square opening is 0.100mm, the pattern is not visible to the naked eye. After being magnified twice by an optical microscope, the direction of the square opening can be correctly determined by the naked eye. The magnification of the optical microscope is set to 2X at this time.

[0016] When the side length of the square opening is 0.050mm, the pattern is not visible to the naked eye. After being magnified four times by an optical microscope, the direction of the square opening can be correctly determined by the naked eye. The magnification of the optical microscope is set to 4X at this time.

[0017] When the side length of the square opening is 0.020 mm, the image is not visible to the naked eye. After being magnified ten times by an optical microscope, the direction of the square opening can be correctly determined by the naked eye. The magnification of the optical microscope is set to 10X at this time; and so on.

[0018] Due to the adoption of the above technical solutions, the beneficial effects of this invention are as follows: This invention provides a new, simplified standard for optical magnification, with a determination method similar to a human visual acuity chart. For the first time, it utilizes the human retina's resolving limit of 0.10-0.20 mm and applies it to the standard of optical magnification, forming a cross-disciplinary research result between medicine and optics. Using a black-background transparent frame for optical magnification effectively avoids electronic magnification such as software calculation corrections. This invention can determine the magnification of optical microscopes, solving the technical problem of the lack of a unified standard for determining optical magnification in the market. Furthermore, it can determine the magnification of optical-electron composite microscopes, eliminating electronic magnification interference. It can also determine the performance differences between different optical magnifying glasses and different optical microscopes. Attached Figure Description

[0019] The following figures are intended only to illustrate and explain the present invention and do not limit the scope of the invention. Wherein:

[0020] Figure 1This is a structural schematic diagram of Embodiment 1 of the present invention;

[0021] Figure 2 This is a magnified view of the second column and second row of the graphic view block in Embodiment 1 of the present invention.

[0022] In the image: 1-Background panel; 2-Graphic view block; 3-Transparent spiral frame; 4-Square opening. Detailed Implementation

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments. In the following detailed description, only certain exemplary embodiments of the invention are described by way of illustration. Undoubtedly, those skilled in the art will recognize that various modifications can be made to the described embodiments without departing from the spirit and scope of the invention. Therefore, the drawings and description are illustrative in nature and not intended to limit the scope of the claims.

[0024] The core principle of this invention is as follows: the limit of human eye resolution is 0.10-0.20mm, meaning the smallest size that the human eye can recognize is 0.10-0.20mm. When the side length of the square opening 4 is 0.20mm, the human eye can correctly determine the opening direction of the transparent loop frame 3. This invention sets the magnification to 1X at this time. When the side length of the square opening 4 is 0.10mm, the human eye cannot correctly determine the opening direction of the transparent loop frame 3. After magnification by 2X using an optical microscope, the human eye can again correctly determine the opening direction of the transparent loop frame 3. This invention sets the magnification to 2X at this time. When the side length of the square opening 4 is 0.05mm, the human eye cannot correctly determine the opening direction of the transparent loop frame 3. After magnification by 4X using an optical microscope, the human eye can again correctly determine the opening direction of the transparent loop frame 3. This invention sets the magnification to 4X at this time. And so on. Therefore, the optical magnification = 0.20 / the side length of the square opening 4.

[0025] Example 1: As Figure 1 and Figure 2 As shown, a tool for determining the optical magnification of an optical microscope includes several columns of graphic blocks drawn on a background plate 1. Each column of graphic blocks includes four graphic blocks 2. The graphic blocks 2 are of equal size, and the spacing between two adjacent graphic blocks 2 is equal. The shapes and sizes of the graphics drawn on the graphic blocks 2 in the same column are the same, but the orientation of the graphics are different. The sizes of the graphics drawn on the graphic blocks 2 in two adjacent columns decrease by a factor of 1.

[0026] like Figure 1 and Figure 2As shown, background panel 1 is a transparent panel, graphic view block 2 is a black square view block, and the graphic is a transparent loop frame 3 with a square opening 4. The side length of the square opening 4 is equal to the line width of the transparent loop frame 3.

[0027] The square opening 4 is located in the middle of one of the sides of the transparent loop frame 3.

[0028] The square view block 2 has a size of 1.200mm*1.200mm, and the distances of each side of the transparent loop frame 3 from the corresponding side distances on the graphic view block 2 are equal.

[0029] When the line width of the transparent loop frame 3 is defined as 0.200mm, it is considered magnified by 1x. Then, when the line width of the transparent loop frame 3 is 0.100mm, 0.050mm, 0.040mm, 0.020mm, and 0.002mm, the corresponding magnification factors are 2x, 4x, 5x, 10x, and 100x, respectively.

[0030] In this embodiment, the graphic view block 2 assembly consists of 10 columns. The side length of the square opening 4 on the first column of graphic view block 2 is 0.200mm. The side lengths of the square opening 4 on the second to tenth columns of graphic view block 2 are 0.100mm, 0.067mm, 0.050mm, 0.040mm, 0.033mm, 0.029mm, 0.025mm, 0.022mm, and 0.020mm, respectively.

[0031] In the same column of graphics, the square openings 4 on the transparent loop frame 3 face different directions. In adjacent columns, the square openings 4 on the transparent loop frame 3 in the same row also face different directions.

[0032] This embodiment, much like an eye chart, can determine the magnification of an optical microscope, solving the technical problem of the lack of a unified standard for determining optical magnification in the market. It can also determine the performance differences between different optical magnifying glasses and different optical microscopes.

[0033] Example 2:

[0034] The technical solution of this embodiment is basically the same as that of Embodiment 1, except that: when the line width of the transparent loop frame 3 is defined as 0.100mm, it is considered a magnification of 1x. Therefore, when the line width of the transparent loop frame 3 is 0.050mm, 0.025mm, 0.020mm, 0.010mm, and 0.001mm, the corresponding magnification factors are 2x, 4x, 5x, 10x, and 100x, respectively. The definition of 0.100mm as a magnification of 1x is based on the fact that the limit of human visual resolution is 0.10-0.20mm. Embodiment 1 uses an upper limit of 0.20mm, while Embodiment 2 uses a lower limit of 0.10mm. Both Embodiment 2 and Embodiment 1 can be used as measurement benchmarks for users to choose from.

[0035] Example 3: This invention also provides a method for determining the optical magnification of an optical microscope. Taking Example 1 as an example, the optical microscope to be tested is observed using the tools provided in the example. When the side length of the square opening 4 is 0.200 mm, the direction of the square opening 4 can be clearly seen with the naked eye, and the magnification is defined as 1X at this time;

[0036] When the side length of the square opening 4 is 0.100mm, the human eye cannot see the pattern clearly. After being magnified twice by an optical microscope, the human eye can correctly determine the direction of the square opening 4. The magnification of the optical microscope is set to 2X at this time.

[0037] When the side length of the square opening 4 is 0.050mm, the human eye cannot see the pattern clearly. After being magnified four times by an optical microscope, the human eye can correctly determine the direction of the square opening 4. The magnification of the optical microscope is set to 4X at this time.

[0038] When the side length of the square opening 4 is 0.020mm, the pattern is not visible to the naked eye. After being magnified ten times by an optical microscope, the human eye can correctly determine the direction of the square opening 4. The magnification of the optical microscope is set to 10X at this time; and so on.

[0039] If the optical microscope can clearly see the 0.020mm square opening 4 in this embodiment, it means that the magnification of the optical microscope is 10X.

[0040] Similarly, when comparing two optical microscopes both labeled 10X, the one that can see the 0.020mm square opening 4 more clearly has higher performance.

[0041] This invention provides a new, simplified standard for the magnification of optical microscopes or magnifying glasses, using a method similar to a human visual acuity chart. For the first time, it utilizes the human retina's resolving limit of 0.1-0.2 mm and applies it to the standard of optical magnification, representing a cross-disciplinary research achievement between medicine and optics. Optical magnification based on a black background and transparent wireframe effectively avoids electronic magnification, such as software calculation corrections. This invention can determine the magnification of optical microscopes, solving the technical problem of the lack of a unified standard for determining optical magnification in the market. It can also determine the magnification of optical-electron hybrid microscopes, eliminating electronic magnification interference. Furthermore, it can determine the performance differences between different optical magnifying glasses and different optical microscopes.

[0042] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A tool for determining the optical magnification of an optical microscope, characterized in that: It includes several columns of graphic view blocks drawn on a background plate (1). Each column of the graphic view block combination includes four graphic view blocks (2). The graphic view blocks (2) are of equal size. The spacing between two adjacent graphic view blocks (2) is equal. The graphic shapes and sizes drawn on the graphic view blocks (2) in the same column are the same, but the orientation of the graphic is different. The size of the graphic drawn on the graphic view blocks (2) in two adjacent columns decreases by a multiple.

2. The tool for determining the optical magnification of an optical microscope as described in claim 1, characterized in that: The background plate (1) is a transparent plate, the graphic block (2) is a black square block, and the graphic is a transparent loop frame (3) with a square opening (4). The side length of the square opening (4) is equal to the line width of the transparent loop frame (3).

3. The tool for determining the optical magnification of an optical microscope as described in claim 2, characterized in that: The square opening (4) is located in the middle of one of the sides of the transparent loop frame (3).

4. The tool for determining the optical magnification of an optical microscope as described in claim 2, characterized in that: The size of the graphic view block (2) is 1.200mm*1.200mm, and the distance from each side of the transparent loop frame (3) to the corresponding side of the graphic view block (2) is equal.

5. A tool for determining the optical magnification of an optical microscope as described in claim 4, characterized in that: When the line width of the transparent loop frame (3) is defined as 0.200mm, it is considered to be magnified by 1x. Then, when the line width of the transparent loop frame (3) is 0.100mm, 0.050mm, 0.040mm, 0.020mm, and 0.002mm, the corresponding magnification factors are 2x, 4x, 5x, 10x, and 100x, respectively.

6. The tool for determining the optical magnification of an optical microscope as described in claim 4, characterized in that: When the line width of the transparent loop frame (3) is defined as 0.100mm, it is considered to be magnified by 1x. Then, when the line width of the transparent loop frame (3) is 0.050mm, 0.025mm, 0.020mm, 0.010mm, and 0.001mm, the corresponding magnification factors are 2x, 4x, 5x, 10x, and 100x, respectively.

7. The tool for determining the optical magnification of an optical microscope as described in claim 4, characterized in that: The graphic block combination has 10 columns. The side length of the square opening (4) on the graphic block (2) in the first column is 0.200mm. The side lengths of the square opening (4) on the graphic block (2) in the second to tenth columns are 0.100mm, 0.067mm, 0.050mm, 0.040mm, 0.033mm, 0.029mm, 0.025mm, 0.022mm, and 0.020mm, respectively.

8. A method for determining the optical magnification of an optical microscope, characterized in that: Using the tool for determining the optical magnification of an optical microscope as described in claim 7, when the side length of the square opening (4) is 0.200 mm, the direction of the square opening (4) can be clearly seen by the human eye, and the magnification is defined as 1X at this time; When the side length of the square opening (4) is 0.100mm, the human eye cannot see the pattern clearly. After being magnified twice by an optical microscope, the human eye can correctly determine the direction of the square opening (4). The magnification of the optical microscope is set to 2X at this time. When the side length of the square opening (4) is 0.050mm, the human eye cannot see the pattern clearly. After being magnified four times by an optical microscope, the human eye can correctly determine the direction of the square opening (4). The magnification of the optical microscope is set to 4X at this time. When the side length of the square opening (4) is 0.020 mm, the human eye cannot see the pattern clearly. After being magnified ten times by an optical microscope, the human eye can correctly determine the direction of the square opening (4). The magnification of the optical microscope is set to 10X at this time; and so on.