Mass spectrum and chromatography combined wine mycotoxin metabolite non-targeted recognition method

By using high-performance liquid chromatography coupled with time-of-flight mass spectrometry, the problem of the inability to identify unknown mycotoxins in existing technologies has been solved, enabling efficient separation and analysis of mycotoxins in wine and ensuring the accuracy and sensitivity of identification.

CN122283031APending Publication Date: 2026-06-26NINGXIA HUI AUTONOMOUS REGION FOOD TESTING RES INST
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Patent Information

Application Number
CN202610161931.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-04
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing mass spectrometry-chromatography techniques mainly rely on targeted detection, which cannot effectively identify unknown fungal toxins and their metabolites. Furthermore, traditional methods involve complex sample pretreatment and long analysis times.

Method used

High-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-MS/MS) was used to achieve non-targeted identification of the mycotoxin ochratoxin A and its metabolites in wine through gradient elution and full-scan mass spectrometry data acquisition, combined with mass spectrometry fragment ion analysis. This included sample pretreatment, mass spectrometry data screening, and fragment ion confirmation.

Benefits of technology

This method enables efficient separation and analysis of mycotoxins in wine, significantly reducing analysis time and operational complexity. It can rapidly identify unknown mycotoxins and their metabolites, improving the separation and sensitivity of detection and ensuring the accuracy of identification.

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Abstract

The invention relates to a non-targeted identification method for mycotoxin metabolites in wine based on mass spectrum and chromatography combination, which is particularly used for detecting and identifying ochratoxin A and metabolites thereof in wine. The steps of sample pretreatment, chromatographic separation, mass spectrometry detection, data screening, fragment ion confirmation and the like are carried out on a wine sample, and non-targeted detection can be carried out on the ochratoxin A and the metabolite thereof under the condition of not depending on a standard substance; in the sample pretreatment stage, an acidified acetonitrile solution is used for extracting a target object, and impurities are removed through salting-out and centrifuging steps; high performance liquid chromatography and time-of-flight mass spectrometry are combined, and a reversed-phase C18 chromatographic column is adopted for gradient elution, so that separation of the ochratoxin A and the metabolite of the ochratoxin A is realized; mass spectrometric analysis uses a full-scanning mode, data are alternately collected in a positive and negative ion mode, high resolution and high sensitivity are guaranteed, the method is suitable for mycotoxin detection in wine and other food, and the food safety supervision requirement is met.
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