A nutrient supplement containing five grain worm oil and its preparation method and application

Abstract

This invention discloses a nutritional supplement containing five-grain insect oil, its preparation method, and its application. The active ingredients of this nutritional supplement are composed of five-grain insect oil, DHA, and phosphatidylserine in a mass ratio of (2-4):(1-3):(1-2), preferably 3:2:1. The preparation method includes mixing and emulsifying five-grain insect oil, DHA, and phosphatidylserine, adding a wall material for microencapsulation, spray drying to obtain microencapsulated powder, and then mixing with excipients to form the target dosage form. The nutritional supplement prepared by this invention can effectively refresh the mind, relieve mental fatigue, improve concentration and memory, and delay cognitive decline, making it suitable for long-term consumption by mental workers, students, and the middle-aged and elderly.

Description

Technical Field

[0001] This invention relates to the field of nutritional supplement technology, specifically to a nutritional supplement containing five-grain insect oil, its preparation method, and its application. Background Technology

[0002] With the fast pace of modern life and increasing work pressure, mental workers, students, and the middle-aged and elderly commonly face problems such as declining memory, poor concentration, and mental fatigue. The brain is one of the organs with the highest energy consumption and oxygen metabolism in the human body, and has extremely high requirements for lipids, proteins, and energy. When nutritional supply is insufficient or the metabolic function of brain cells declines, it easily leads to mental fatigue, and long-term accumulation may lead to cognitive decline.

[0003] Therefore, developing nutritional supplements that can effectively improve brain function and relieve brain fatigue is of great significance. Currently, there are many types of brain nutrition products on the market, with common functional ingredients including docosahexaenoic acid (DHA), phosphatidylserine (PS), and fish oil. However, these products generally suffer from drawbacks such as limited ingredient variety, insufficient synergistic effects, and low bioavailability, making it difficult to meet the comprehensive and multi-layered nutritional needs of the brain. Summary of the Invention

[0004] In view of this, the present invention provides a nutritional supplement containing five-grain insect oil, its preparation method and application. The nutritional supplement is composed of five-grain insect oil, DHA and phosphatidylserine, and has the effects of refreshing the mind, relieving brain fatigue, improving concentration and memory. Moreover, the components have synergistic effects, high bioavailability and are suitable for long-term use.

[0005] The technical solution provided by this invention is as follows: In a first aspect, the present invention provides a nutritional supplement containing five-grain insect oil, wherein the active ingredients are composed of five-grain insect oil, DHA, and phosphatidylserine in a mass ratio of (2-4):(1-3):(1-2).

[0006] Furthermore, the active ingredients consist of five grain insect oil, DHA, and phosphatidylserine in a mass ratio of 3:2:1.

[0007] Secondly, the present invention provides a method for preparing a nutritional supplement containing five-grain insect oil as described in any of the preceding claims, comprising the following steps: S1. Weigh out the five grain insect oil, DHA and phosphatidylserine, mix them evenly to obtain an oil phase mixture; S2. Add an aqueous phase containing an emulsifier to the oil phase mixture obtained in step S1, and perform high-shear emulsification to form a uniform and stable emulsion; S3. Add wall material to the emulsion obtained in step S2 for microencapsulation and embedding treatment, and then spray dry to obtain microencapsulated powder; S4. Mix the microcapsule powder obtained in step S3 with excipients to prepare the corresponding dosage form.

[0008] Furthermore, the emulsifier mentioned in step S2 is selected from at least one of soybean lecithin, glyceryl monostearate, glyceryl distearate, and sodium octenyl succinate starch.

[0009] Furthermore, the high-shear emulsification in step S2 is emulsification at 10,000-15,000 rpm for 10-15 minutes.

[0010] Furthermore, the wall material mentioned in step S3 is selected from at least one of maltodextrin, gum arabic, modified starch, gelatin, sodium alginate, and chitosan.

[0011] Furthermore, in step S3, the inlet air temperature of the spray dryer is 160-180℃, and the outlet air temperature is 80-90℃.

[0012] Furthermore, the dosage form described in step S4 is a soft capsule, granule, oral emulsion, or tablet.

[0013] Thirdly, the present invention provides the application of a nutritional supplement containing five-grain insect oil as described in any of the preceding claims in the preparation of products that improve memory, relieve brain fatigue, enhance concentration, and delay cognitive decline.

[0014] The beneficial effects of this invention are as follows: This invention provides a nutritional supplement containing five-grain insect oil, which is a compound of five-grain insect oil, DHA, and phosphatidylserine in a specific mass ratio. The three components work synergistically. Five-grain insect oil is rich in various unsaturated fatty acids and nervonic acid, which, together with DHA and phosphatidylserine, can effectively nourish nerve cells, enhance nerve signal transmission efficiency, and promote brain energy metabolism, thereby significantly improving central excitability, relieving brain fatigue, and improving concentration. Animal experiments show that the nutritional supplement prepared in this invention can significantly increase the number of spontaneous activities in mice, exhibiting good effects in refreshing the mind and relieving brain fatigue. Simultaneously, five-hole attention behavior tests show that this nutritional supplement can significantly improve the hit rate and accuracy of mice, and reduce the false alarm rate and omission rate, indicating that it can effectively improve attention levels and enhance concentration. Furthermore, this invention uses a microencapsulation process to prepare microencapsulated powder, effectively protecting the stability of the active ingredients and improving their bioavailability. The nutritional supplement prepared by this invention is suitable for long-term use by mental workers, students and the elderly. It has broad application prospects in improving memory, relieving mental fatigue, enhancing concentration and delaying cognitive decline. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only a part of the embodiments of this invention, not all of them. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention. Experimental methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the manufacturer. Unless otherwise stated, percentages and parts are weight percentages and parts by weight.

[0016] Unless otherwise specified, the technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. It should be noted that the terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the exemplary embodiments of this application.

[0017] Preparation Example A method for preparing five-grain insect oil includes the following steps: After the dried grain insects were crushed and passed through a 40-mesh sieve, the crushed insects were placed in a supercritical extraction vessel. The extraction pressure was set to 25 MPa, the extraction temperature to 43℃, and the CO2 flow rate to 20 L / h. The extraction was carried out for 3 hours, and the extract was collected to obtain crude oil from the grain insects. According to the test results from PONY Testing Group (report number: B1G205033B1F10I3053), the total amount of unsaturated fatty acids in Wugu Worm Oil is 49.1g / 100g, of which the content of EPA is 2.16g / 100g and the content of nervonic acid is 0.0104g / 100g. Example

[0018] A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh out the five grain insect oil, DHA and phosphatidylserine in a mass ratio of 3:2:1, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture; S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Add 20% maltodextrin and 10% gum arabic as wall material to the emulsion obtained in step S2, continue stirring for 30 minutes to fully dissolve and disperse it, and then spray dry it with an inlet air temperature of 170°C and an outlet air temperature of 85°C to obtain microcapsule powder. S4. Mix the microcapsule powder obtained in step S3 with microcrystalline cellulose and magnesium stearate at a mass ratio of 95:4:1, fill the capsule shells, and make soft capsules with a net weight of 500mg of contents per capsule. Example

[0019] A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh out the five grain insect oil, DHA and phosphatidylserine in a mass ratio of 3:2:1, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture; S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Add 20% maltodextrin and 10% gum arabic as wall material to the emulsion obtained in step S2, continue stirring for 30 minutes to fully dissolve and disperse it, and then spray dry it with an inlet air temperature of 170°C and an outlet air temperature of 85°C to obtain microcapsule powder. S4. Mix the microcapsule powder obtained in step S3 with lactose, microcrystalline cellulose and cross-linked povidone in a mass ratio of 5:10:3:1. Add 10% povidone K30 solution to make a soft mass, granulate it through an 18-mesh sieve, dry it at 55℃ until the moisture content is <5%, granulate it through a 14-mesh sieve, add 1% magnesium stearate and mix it evenly to obtain the granules.

[0020] Comparative Example 1 A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh DHA and phosphatidylserine at a mass ratio of 2:1, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture; S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Add 20% maltodextrin and 10% gum arabic as wall material to the emulsion obtained in step S2, continue stirring for 30 minutes to fully dissolve and disperse it, and then spray dry it with an inlet air temperature of 170°C and an outlet air temperature of 85°C to obtain microcapsule powder. S4. Mix the microcapsule powder obtained in step S3 with microcrystalline cellulose and magnesium stearate at a mass ratio of 95:4:1, fill the capsule shells, and make soft capsules with a net weight of 500mg of contents per capsule.

[0021] Compared to Example 1, this comparative example did not contain any grain insect oil.

[0022] Comparative Example 2 A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh out the five grain insect oil and phosphatidylserine at a mass ratio of 3:1, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture; S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Add 20% maltodextrin and 10% gum arabic as wall material to the emulsion obtained in step S2, continue stirring for 30 minutes to fully dissolve and disperse it, and then spray dry it with an inlet air temperature of 170°C and an outlet air temperature of 85°C to obtain microcapsule powder. S4. Mix the microcapsule powder obtained in step S3 with microcrystalline cellulose and magnesium stearate at a mass ratio of 95:4:1, fill the capsule shells, and make soft capsules with a net weight of 500mg of contents per capsule.

[0023] This comparative example, compared to Example 1, did not contain added DHA.

[0024] Comparative Example 3 A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh out the five grain insect oil and DHA in a mass ratio of 3:2, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture; S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Add 20% maltodextrin and 10% gum arabic as wall material to the emulsion obtained in step S2, continue stirring for 30 minutes to fully dissolve and disperse it, and then spray dry it with an inlet air temperature of 170°C and an outlet air temperature of 85°C to obtain microcapsule powder. S4. Mix the microcapsule powder obtained in step S3 with microcrystalline cellulose and magnesium stearate at a mass ratio of 95:4:1, fill the capsule shells, and make soft capsules with a net weight of 500mg of contents per capsule.

[0025] Compared to Example 1, this comparative example did not contain phosphatidylserine.

[0026] Comparative Example 4 A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh out the five grain insect oil, DHA and phosphatidylserine in a mass ratio of 1:4:3, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture. S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Add 20% maltodextrin and 10% gum arabic as wall material to the emulsion obtained in step S2, continue stirring for 30 minutes to fully dissolve and disperse it, and then spray dry it with an inlet air temperature of 170°C and an outlet air temperature of 85°C to obtain microcapsule powder. S4. Mix the microcapsule powder obtained in step S3 with microcrystalline cellulose and magnesium stearate at a mass ratio of 95:4:1, fill the capsule shells, and make soft capsules with a net weight of 500mg of contents per capsule.

[0027] Compared to Example 1, the mass ratio of five-grain insect oil, DHA, and phosphatidylserine in this comparative example is 1:4:3.

[0028] Comparative Example 5 A method for preparing a nutritional supplement containing five-grain insect oil includes the following steps: S1. Weigh out the five grain insect oil, DHA and phosphatidylserine in a mass ratio of 3:2:1, place them in a mixing container, and stir and mix them evenly in a water bath at 45°C under nitrogen protection to obtain an oil phase mixture; S2. Take another deionized water and add sodium octenyl succinate starch, accounting for 4% of the total mass of the oil phase, as an emulsifier. Heat to 65°C and stir to dissolve, which will be the aqueous phase. Slowly add the oil phase mixture to the aqueous phase while stirring, and emulsify at 12000 rpm for 12 minutes using a high-shear emulsifier to form a uniform and stable emulsion. S3. Concentrate the emulsion obtained in step S2 under reduced pressure to a paste to obtain a paste; S4. Mix the paste obtained in step S3 with microcrystalline cellulose and magnesium stearate at a mass ratio of 95:4:1, fill the mixture into capsule shells, and make soft capsules with a net weight of 500mg of contents per capsule.

[0029] Compared to Example 1, this comparative example did not undergo microencapsulation.

[0030] Experiment Example 1: Autonomous Activity Test Eighty SPF-grade male Kunming mice, weighing 18-22g, were randomly divided into eight groups of ten mice each after one week of acclimatization. The control group was administered physiological saline by gavage, while the experimental groups were administered the corresponding nutritional supplements prepared in the examples and comparative examples by gavage at a dose of 1g / kg, once daily for 14 consecutive days. During the experiment, all mice were provided with normal food and water. Two hours after the last administration on day 14, the mice were placed in a spontaneous activity measuring device for 5 minutes to acclimatize. The number of spontaneous activities was recorded within 10 minutes, and then recorded every 30 minutes for a total of three measurements. The average value was taken as the number of spontaneous activities for each group. The experimental results are shown in Table 1.

[0031] Table 1. Effects of various nutritional supplements on spontaneous activity in mice.

[0032] As shown in Table 1, compared with the blank control group, the number of spontaneous activities in mice in each treatment group was significantly increased. Specifically, the number of spontaneous activities in the Example 1 and Example 2 groups was significantly higher than that in the comparative groups, indicating that the nutritional supplement of the present invention has a significant central nervous system stimulant effect and can effectively refresh the mind and relieve brain fatigue. Comparative groups 1-3, lacking one of the components of five-grain insect oil, DHA, or phosphatidylserine, had significantly lower numbers of spontaneous activities than the Example groups, indicating a synergistic effect among the three. The effect of comparative group 4 was also less than that of the Example groups. Comparative group 5, without microencapsulation treatment, also had a lower effect than the Example groups, indicating that microencapsulation can effectively improve the stability and bioavailability of the components, further enhancing the product's efficacy.

[0033] Experiment Example 2: Test to Improve Attention A five-hole cognitive behavior testing box was used to conduct animal behavioral tests. Five testing holes are arranged in an arc shape on the rear wall of the box. Each hole is equipped with an infrared beam detection module and a visual stimulus light source. The device is equipped with an automatic food reward dispensing system. Eighty SPF-grade male Kunming mice, weighing between 18 and 22 g, were selected. Before the experiment, all mice underwent a 14-day acclimatization training period to familiarize them with the testing procedure. In the initial training phase, only one hole light source was activated to guide the mice to complete the target exploration response. As training progressed, the system switched to the complete five-hole mode, simultaneously setting two types of stimulus conditions: single target stimulus (randomly illuminating one hole light source) and global non-target stimulus (simultaneously illuminating all five holes light sources), establishing a behavioral paradigm of target stimulus response and non-target stimulus inhibition in the mice. Mice were considered to have achieved training goals when they consistently achieved a hit rate of no less than 80%, a correct rejection rate of no less than 80%, and a fluctuation range of less than 10% in each behavioral indicator for three consecutive days.

[0034] Eighty trained mice were randomly divided into eight groups of ten each: a blank control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. The blank control group was administered an equal volume of physiological saline by gavage, while each treatment group was administered the corresponding sample by gavage at a dose of 1 g / kg·d for 14 consecutive days. Twenty minutes after the last administration, a five-hole attention behavior test was formally conducted. Each test lasted 40 minutes, with a total of 200 response trials, and target stimuli and non-target stimuli each accounted for 50%. The number of hits, misses, false alarms, and correct rejections were automatically recorded and counted using a behavioral data acquisition system. The hit rate, false alarm rate, miss rate, and accuracy rate were further calculated to evaluate the attention level of each group of mice.

[0035] Table 2. Effects of various nutritional supplements on the concentration of mice.

[0036] As shown in Table 2, compared with the blank control group, the hit rate and accuracy of mice in Example 1 and Example 2 groups were significantly improved, while the false alarm rate and omission rate were significantly reduced, indicating that the nutritional supplement of the present invention can effectively improve the concentration level of mice. Among them, Comparative Examples 1-3, lacking one of the components of five-grain insect oil, DHA, or phosphatidylserine, had significantly lower hit rates and accuracy than the Example groups, and significantly higher false alarm rates and omission rates, indicating that the three have a synergistic effect. The concentration improvement effect of Comparative Example 4 was also not as good as that of the Example groups. Comparative Example 5, without microencapsulation treatment, had a significantly worse concentration improvement effect than the Example groups, indicating that the microencapsulation process can effectively improve the stability and bioavailability of active ingredients, thereby enhancing the concentration improvement effect of the product.

[0037] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A nutritional supplement containing five-grain insect oil, characterized in that, The active ingredients consist of five grain insect oil, DHA, and phosphatidylserine in a mass ratio of (2-4):(1-3):(1-2).

2. The nutritional supplement containing five-grain insect oil according to claim 1, characterized in that, The active ingredients consist of five grain insect oil, DHA, and phosphatidylserine in a mass ratio of 3:2:

1.

3. A method for preparing a nutritional supplement containing five-grain insect oil as described in any one of claims 1 or 2, characterized in that, Includes the following steps: S1. Weigh out the five grain insect oil, DHA and phosphatidylserine, mix them evenly to obtain an oil phase mixture; S2. Add an aqueous phase containing an emulsifier to the oil phase mixture obtained in step S1, and perform high-shear emulsification to form a uniform and stable emulsion; S3. Add wall material to the emulsion obtained in step S2 for microencapsulation and embedding treatment, and then spray dry to obtain microencapsulated powder; S4. Mix the microcapsule powder obtained in step S3 with excipients to prepare the corresponding dosage form.

4. The method for preparing a nutritional supplement containing five-grain insect oil according to claim 3, characterized in that, The emulsifier mentioned in step S2 is selected from at least one of soybean lecithin, glyceryl monostearate, glyceryl distearate, and sodium octenyl succinate starch.

5. The method for preparing a nutritional supplement containing five-grain insect oil according to claim 3, characterized in that, The high-shear emulsification in step S2 is emulsification at 10,000-15,000 rpm for 10-15 minutes.

6. The method for preparing a nutritional supplement containing five-grain insect oil according to claim 3, characterized in that, The wall material mentioned in step S3 is selected from at least one of maltodextrin, gum arabic, modified starch, gelatin, sodium alginate, and chitosan.

7. The method for preparing a nutritional supplement containing five-grain insect oil according to claim 3, characterized in that, The inlet air temperature of the spray drying process in step S3 is 160-180℃, and the outlet air temperature is 80-90℃.

8. The method for preparing a nutritional supplement containing five-grain insect oil according to claim 3, characterized in that, The dosage form mentioned in step S4 is a soft capsule, granule, oral emulsion, or tablet.

9. The use of a nutritional supplement containing five-grain insect oil as described in any one of claims 1 or 2 in the preparation of products that improve memory, relieve brain fatigue, enhance concentration, and delay cognitive decline.

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