A device for inducing periphyton to proliferate in a rice field, periphyton and application

Abstract

This invention discloses a device for inducing the amplification of periphyte organisms in paddy fields, comprising a hollow amplification cavity enclosed by a shell. An artificial carrier, an aeration device, a light module, and a temperature control module are arranged within the amplification cavity. The artificial carrier is a porous material with a pore size of 80-250 μm, a porosity of 85-95%, and a total pore area of ​​0.20-0.80 m². 2 / g. Furthermore, this invention also provides a pericarp biofilm and its applications. The beneficial effects are: the artificial carrier has abundant microbial attachment points, which can promote the growth and expansion of the biofilm and prevent detachment; it can also effectively reduce the mechanical shear force of water flow on microorganisms, intercept suspended organic matter in sewage, provide nutrients for microorganisms, lower the surface energy barrier for microbial attachment, and make it easier for various bacterial and algal communities to colonize, achieving rapid biofilm formation and mass production.

Description

Technical Field

[0001] This invention relates to the field of water treatment technology, specifically to a device for inducing the amplification of periphytes in paddy fields, the periphytes themselves, and their applications. Background Technology

[0002] As a type of microbial community that attaches and grows on the surface of aquatic substrates, *Zygophyllum* has significant application value in water purification, ecological restoration, and resource utilization. Large-scale, standardized cultivation is a key prerequisite for its engineering applications. While existing *Zygophyllum* cultivation devices can simulate natural growth environments to some extent, they still have significant shortcomings in terms of growth rate and adaptability to large-scale cultivation.

[0003] For example, CN105624025B discloses a cultivation device for periclump organisms, comprising: a cultivation chamber; a periclump attachment system disposed within the cultivation chamber for periclump organisms to attach and grow; a lighting system disposed within the cultivation chamber and above the periclump attachment system for providing light and heat sources for the growth of periclump organisms; a water supply system for supplying water to the periclump attachment system for providing a water flow environment for the growth of periclump organisms; a water quality parameter detection system disposed within the cultivation chamber for detecting the water quality conditions for the growth of periclump organisms; and a support system for supporting and adjusting the inclination angle of the cultivation chamber relative to the horizontal plane, for changing the channel conditions of the periclump attachment system within the cultivation chamber by adjusting the inclination angle of the cultivation chamber. The cultivation chamber can be a cuboid or a cube. The water contains at least three elements: carbon, nitrogen, and phosphorus. In particular, the carbon content in the water is 1-10 mg / L, preferably 5-8 mg / L. In particular, the nitrogen content in the water is 0.5-1.5 mg / L, preferably 0.8-1.2 mg / L. In particular, the phosphorus content in the water is 0.1-0.2 mg / L, preferably 0.15 mg / L. In particular, trace elements such as iron (Fe), potassium (K), boron (B), manganese (Mn), zinc (Zn), copper (Cu), and cobalt (Co) may also be added to the water. In particular, the flow velocity of the water is 0.2-1 m / s, preferably 0.5-1 m / s. The periclump bio-attachment system is located at the bottom of the incubator and includes a coarse sand layer and pebbles on top of it. In particular, the thickness of the coarse sand layer is 15-20 cm, and the particle size of the coarse sand in the layer is 0.5-2 mm. In particular, the major axis diameter of the pebbles is 15-20 cm, the minor axis diameter is 10-15 cm, and the thickness of the pebbles is 1-5 cm. Specifically, the pebbles are randomly laid flat on the coarse sand. The coarse sand layer is designed to simulate the natural riverbed environment for the growth of periphytes, while the pebble layer provides a habitat for them to attach and grow. The lighting system, located at the top of the incubator, includes a lighting device and a power supply. Specifically, the light intensity of the lighting device is adjustable, allowing for adjustments to suit the growth needs of the periphytes. Specifically, the lighting device provides a light intensity of 0-50000 lx. Specifically, the power supply is connected to an indoor power outlet to power the lighting device.

[0004] The above scheme involves randomly laying pebbles, resulting in large differences in the size of the gaps and the exposed area. This leads to extremely uneven growth of the surrounding organisms, making them easy to demold and impossible to achieve mass production.

[0005] CN104789472A discloses an apparatus for culturing peritrichous organisms, comprising a peritrichous culture tank. The apparatus is characterized by being made of a transparent material, with a WC mother liquor tank above it for replenishing the culture medium; a drain pipe at the bottom of the culture tank; a carrier within the culture tank; and a lighting and aeration mechanisms. Further improvements to the apparatus include the following optimizations: the WC mother liquor tank replenishes the culture medium to the peritrichous culture tank via a delivery pipe, valves, and a collection tank; the valves can be automatic valves controlled by float valves; the lighting mechanism uses LED lights mounted on the side of the culture tank; the aeration mechanism includes an air pump that aerates the culture medium in the culture tank via a main air pipe, branch air pipes, and aeration stones at the lower end of the branch air pipes; and a float valve control tank is located below the drain pipe at the bottom of the culture tank.

[0006] The above solution relies solely on a float valve to control the replenishment of liquid. Fluctuations in water quality, biofilm adhesion, and blockage by impurities can cause the float valve to malfunction, resulting in fluctuating liquid levels and an unstable growth environment for periphytes, which is not conducive to rapid biofilm formation and mass production.

[0007] Furthermore, CN120442361A discloses an apparatus for culturing peritrichous organisms, comprising a culture chamber body, a control panel connected to the top of the culture chamber body, and a culture container connected inside the culture chamber body. An arc-shaped box is installed on the inner wall of the culture chamber body, and a light lamp is connected to the outer wall of the arc-shaped box. The light lamp is located above the culture container, and a lamp shaft seat is fixedly connected to the end of the light lamp. An arc-shaped cover is fixedly installed on the outer side of the arc-shaped box. An arc-shaped groove is formed on the top of the arc-shaped cover and the arc-shaped box. A chain and two sprockets are connected between the arc-shaped cover and the arc-shaped box. The two sprockets are located at the two ends of the arc-shaped box, and the chain is sleeved on the outer side of the two sprockets. The lamp shaft seat passes through the arc-shaped groove and is fixedly connected to the chain plate. Further, a fixed shaft is fixedly connected to the axis of the two sprockets. The fixed shaft passes through the arc-shaped box and is rotatably connected to it. A drive shaft is fixedly connected to the outer side of one of the fixed shafts, and a motor fixedly connected to the drive shaft is fixedly installed on the outer wall of the culture chamber body. Furthermore, the illumination lamp is a long tube with an irradiation wavelength of 400-700nm. Furthermore, a top plate is fixedly connected to the top surface of the arc-shaped cover, and an inner plate is fixedly connected to the inner wall of the arc-shaped box. Multiple vertical plates are fixedly connected to the top surfaces of both the inner and top plates. Both the top and inner plates are two-sectioned. Teeth are fixedly connected to the inner walls of the multiple vertical plates on the front half of the top surface of the top plate and the inner walls of the multiple vertical plates on the rear half of the top surface of the inner plate. The teeth on the multiple vertical plates on the front half of the top surface of the top plate and the teeth on the multiple vertical plates on the rear half of the top surface of the inner plate are symmetrically and staggered. A knob for adjusting the brightness of the illumination lamp is fixedly installed on the outer side of the lamp shaft seat. A gear connected to the knob is sleeved on the outer side of the lamp shaft seat, and the gear meshes with the teeth on the inner walls of the multiple vertical plates. A first switch and a second switch, which can be opened and closed by the lamp shaft seat, are fixedly connected to the bottom of the arc-shaped box at both ends of the arc.

[0008] In the above scheme, the reciprocating motion of the motor and sprocket chain generates continuous micro-vibrations, which is not conducive to the initial attachment and colonization of periclumps. In particular, the newly formed weak biofilm is very easy to fall off due to vibration, resulting in slow biofilm formation and difficulty in mass production. Summary of the Invention

[0009] The purpose of this invention is to provide a device, the perianth organisms, and their applications for inducing the amplification of rice paddy periphytes that can be rapidly attached to a film, achieve mass production, and are not easily detached from the film.

[0010] To achieve the above objectives, the basic solution of the present invention provides a device for inducing the amplification of periphytes in paddy fields, comprising a hollow amplification cavity enclosed by a shell, wherein an artificial carrier, an aeration device, a light module, and a temperature control module are arranged within the amplification cavity. The artificial carrier is a porous material with a pore size of 80-250 μm, a porosity of 85-95%, and a total pore area of ​​0.20-0.80 m².2 / g.

[0011] The beneficial effects of this basic scheme are as follows: the artificial carrier has abundant microbial attachment points, which can promote the growth and expansion of biofilm and make it less prone to detachment; it can also effectively reduce the mechanical shear force of water flow on microorganisms, intercept suspended organic matter in sewage, provide nutrients for microorganisms, reduce the surface energy barrier for microbial attachment, and make it easier for various bacteria and algae to colonize, so as to achieve rapid biofilm formation and mass production.

[0012] Preferably, the artificial carrier is a sponge with a pore size of 100-170 μm, a porosity of 91-93.5%, and a total pore area of ​​0.35-0.52 m². 2 / g. More preferably, the pore size is 166.21 μm, the porosity is 92.73%, and the total pore area is 0.512 m². 2 / g. The sponge with this parameter was selected as the artificial carrier. Its porous structure provides a large specific surface area, which can provide sufficient attachment sites for peripheral organisms, resulting in fast biofilm formation and high biomass loading. Its hydrophilicity and excellent biocompatibility are conducive to the colonization of algae, bacteria and other microorganisms. At the same time, its pores are permeable, have high mass transfer efficiency, are not easy to clog and can automatically renew the biofilm, ensuring biological activity and preventing biofilm detachment.

[0013] Preferably, the artificial carriers are arranged in multiple layers with spacing within the amplification chamber. Through a rational spatial layout and water flow distribution design, the device ensures that the artificial carriers can uniformly contact the environment and microbial communities, avoiding the low efficiency of traditional single-strip biofilm attachment methods and enabling rapid biofilm attachment and mass production.

[0014] Preferably, the aeration device includes an aeration disc with multiple aeration holes, the diameter of which is 50-100μm and the aeration flow rate is adjustable.

[0015] Preferably, the illumination module is an adjustable LED with an illumination intensity between 10,000 and 50,000 lux.

[0016] Preferably, the light cycle is 12 hours of light / 12 hours of darkness. This ensures that algae can fully photosynthesize during the light period, synthesizing organic matter, providing carbon and oxygen sources for heterotrophic microorganisms, and promoting rapid growth of the surrounding biological community and stable biofilm formation. At the same time, it allows microorganisms to carry out respiratory metabolism during the dark period, reducing photoinhibition and photooxidative damage, avoiding excessive photoaging of algal cells. It also makes the physiological activity rhythm of the algae-bacteria-protozoa community closer to the natural environment, resulting in more balanced metabolism, stronger overall activity, community structure stability, and pollutant removal capacity, and is more conducive to rapid biofilm formation and mass production.

[0017] Preferably, the temperature control module is used to maintain the temperature between 25-35°C.

[0018] Preferably, the amplification chamber is also equipped with an ORP probe and a pH probe. Based on the design of the ORP probe and pH probe, light intensity, aeration rate, temperature, etc. can be adjusted in real time to accelerate the migration and adsorption of microorganisms in the water to the surface of the artificial carrier, significantly shorten the biofilm formation cycle, and greatly increase the biofilm biomass. This allows the artificial carrier to have a stronger biological treatment capacity in a shorter time, which is conducive to achieving mass production.

[0019] Preferably, a display screen is provided on the outside of the housing for receiving and displaying monitoring data from the ORP probe and pH probe.

[0020] Secondly, the present invention provides a periphyte, which is cultured by the device for inducing the amplification of periphytes in paddy fields as described in any of the above-mentioned schemes.

[0021] Thirdly, the present invention also provides an apparatus for inducing the amplification of periphytes in paddy fields as described in any of the above embodiments and / or the application of periphytes in the denitrification treatment of low C / N biogas slurry wastewater.

[0022] The present invention has the following beneficial effects: 1. Artificial carriers have abundant microbial attachment points, which can promote the growth and expansion of biofilms and make them less prone to detachment; they can also effectively reduce the mechanical shear force of water flow on microorganisms, intercept suspended organic matter in sewage, provide nutrients for microorganisms, reduce the surface energy barrier for microbial attachment, and make it easier for various bacteria and algae to colonize, thus achieving rapid biofilm formation and mass production.

[0023] 2. Porous artificial carriers can provide a stable environment for microorganisms to attach and amplify, effectively solving the problems of easy detachment and slow amplification of microorganisms and providing support for their degradation of pollutants. Among them, the preferred sponge material is chemically stable, non-toxic and harmless, with moderate density that can swing with water flow, ensuring that microorganisms attach firmly and have sufficient contact, avoiding secondary pollution. It also has the advantages of impact resistance, easy cutting and installation, and low cost, making it suitable for laboratory culture and actual water body remediation and sewage treatment projects.

[0024] 3. The present invention has a simple structure, and in particular, the consumables are made of low-cost and readily available porous or sponge materials. It is easy to operate and maintain, which can reduce the investment of manpower and time. The overall cost is low, and it is both practical and economical, making it suitable for large-scale promotion. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the present invention.

[0026] Figure 2 This is a schematic diagram of another embodiment of the present invention, namely, Example 1. Detailed Implementation

[0027] The rapid development of large-scale livestock and poultry farming has led to a continuous increase in biogas slurry production. While anaerobic digestion can efficiently degrade organic matter in biogas slurry, it also promotes the conversion of a large amount of organic nitrogen into inorganic nitrogen, resulting in a significant increase in the concentrations of NH4+-N and NO3--N in the biogas slurry. Meanwhile, available carbon sources are relatively scarce, forming typical low carbon-to-nitrogen ratio (C / N) wastewater. Although biogas slurry can be used as a nutrient resource for returning to the fields, its high nitrogen, low C / N ratio, and rapid nutrient release characteristics easily lead to nitrogen leaching and runoff pollution in rice-growing areas in the south, exacerbating agricultural non-point source pollution and increasing the emission risks of greenhouse gases such as CO2 and N2O. Therefore, how to effectively control nitrogen loss and reduce greenhouse gas emissions while promoting the resource utilization of biogas slurry has become a key issue that urgently needs to be addressed in the fields of safe biogas slurry return to the fields and agricultural non-point source pollution control.

[0028] Periphytic biofilms, as microbial aggregates widely distributed at the water-soil interface, have shown significant potential in pollutant removal and carbon and nitrogen cycling. Related studies have shown that periphytic biofilms can effectively remove nitrogen, phosphorus, and organic pollutants from water bodies through adsorption, assimilation, and biotransformation. They can also fix CO2 through photosynthesis, playing a crucial role in environmental buffering and homeostasis maintenance in ecosystems such as paddy fields. From a nitrogen cycle perspective, periphytic biofilms can not only assimilate inorganic nitrogen through algae and autotrophic microorganisms, but the aerobic-anoxic microenvironment they create can also support various nitrogen transformation processes such as nitrification, denitrification, and anaerobic ammonium oxidation, demonstrating outstanding potential in nitrogen retention and speciation.

[0029] Peripheral biofilms, composed of multiple species including algae, bacteria, and fungi, play a crucial role in the nitrogen and phosphorus cycle, such as photosynthetic nitrogen fixation and nutrient buffering. When applied to the treatment of nitrogen pollution from agricultural non-point source wastewater, they can significantly improve treatment efficiency and convert pollutants into recyclable substances. However, under natural conditions, peripheral biofilms require a long time to develop from initial attachment to maturity, and the limited specific surface area of ​​their natural carriers (such as rocks and plant stems and leaves) results in a low biofilm content per unit area. This limitation restricts the efficiency of peripheral biofilms in absorbing and utilizing nutrients such as nitrogen and phosphorus in water, thus affecting their water purification effect and hindering their practical application in biogas slurry treatment.

[0030] Therefore, this embodiment provides a device for inducing the amplification of periclump organisms. By optimizing the biofilm attachment device, it can quickly attach biofilms, achieve mass production, and prevent biofilm detachment, so as to better apply it to the denitrification treatment of low C / N biogas slurry wastewater.

[0031] The following detailed description illustrates the specific implementation method: The reference numerals in the accompanying drawings are as follows: 1-shell; 2-artificial carrier; 3-water inlet; 4-support; 41-support hole; 5-aeration disc; 6-aeration inlet; 7-sludge discharge port; 8-sampling port; 9-water outlet / sampling port; 10-adjustable LED; 11-cover; 12-pH probe; 13-pH display screen; 14-ORP probe; 15-ORP display screen; 16-temperature control module; 17-exhaust port; 18-amplification chamber.

[0032] The device for inducing the amplification of periphyte organisms in paddy fields provided in this embodiment includes a hollow amplification cavity 18 enclosed by a shell 1. An artificial carrier 2, an aeration device, a light module, and a temperature control module 16 are arranged within the amplification cavity 18. The artificial carrier 2 is a porous material with a pore size of 80-250 μm, a porosity of 85-95%, and a total pore area of ​​0.20-0.80 m². 2 / g.

[0033] Even better, the artificial carrier 2 is a sponge with a pore size of 100-170 μm, a porosity of 91-93.5%, and a total pore area of ​​0.35-0.52 m². 2 / g.

[0034] Even better, the artificial carrier 2 is arranged in multiple layers with spacing within the amplification cavity 18.

[0035] Even better, the aeration device includes an aeration disc 5, which is provided with multiple aeration holes with a diameter of 50-100μm and an adjustable aeration flow rate.

[0036] Even better, the lighting module is an adjustable LED10 with a light intensity between 10,000 and 50,000 lux.

[0037] Even better, the light cycle is 12 hours of light / 12 hours of darkness.

[0038] Even better, the temperature control module 16 is used to maintain the temperature between 25-35°C.

[0039] Even better, the amplification chamber 18 is also equipped with an ORP probe 14 and a pH probe 12.

[0040] Even better, a display screen is provided on the outside of the housing 1 to receive and display the monitoring data of the ORP probe 14 and the pH probe 12.

[0041] Secondly, this embodiment provides periphytes, which are cultured using the device for inducing the amplification of periphytes in paddy fields provided in any of the above embodiments.

[0042] In addition, this embodiment also provides an apparatus for inducing the amplification of paddy field periphytes as provided in any of the above embodiments and / or the application of periphytes in the denitrification treatment of low C / N biogas slurry wastewater.

[0043] Example 1 The device for inducing the amplification of periphytes in rice paddies provided in this embodiment is basically as shown in the attached figure. Figure 1 , 2 As shown, its structure consists of a shell 1 and a bracket 4 set at the bottom of the shell 1 to support the entire device. The overall structure is compact, easy to install and maintain, and can be adapted to the needs of use in paddy fields.

[0044] The shell 1 has a hollow interior, forming an amplification chamber 18 for the periclump organisms, providing a closed and controllable environment for their attachment, growth, and amplification. A removable cover 11 is located on top of the amplification chamber 18. The cover 11 is bolted to the shell 1, facilitating later opening of the chamber for artificial carrier replacement, equipment maintenance, and internal cleaning. The cover 11 has an exhaust port 17 that connects the inside and outside, used to expel gases produced by microbial metabolism within the amplification chamber 18, preventing excessive pressure within the chamber from affecting device operation and microbial growth. Simultaneously, an adjustable LED 10 is installed on the inner side of the cover 11. Its core function is to provide suitable lighting conditions for the periclump organisms within the amplification chamber 18. Preferably, the adjustable LED 10 provides adjustable illumination between 10,000 and 50,000 lux, allowing flexible adjustment of light intensity according to the light requirements of different growth stages of the periclump organisms, ensuring efficient growth. In this embodiment, a 12-hour light / 12-hour dark cycle is preferred.

[0045] A thermostatic module 16 is installed inside the amplification chamber 18 to maintain a stable water temperature within the chamber, providing a suitable temperature environment for the growth of periphytes. Preferably, the thermostatic module 16 has a strip-shaped structure, and the central axis of the amplification chamber 18 coincides with the center line of the thermostatic module 16, ensuring uniform temperature distribution within the chamber and preventing localized temperature differences from affecting the growth of periphytes. Simultaneously, a certain gap is left between the thermostatic module 16 and the bottom of the amplification chamber 18, meaning the thermostatic module 16 does not extend to the bottom of the amplification chamber 18. This design prevents the thermostatic module 16 from obstructing the aeration structure below, ensuring uniform aeration, and also provides space for bottom water flow and sludge deposition.

[0046] Within the amplification chamber 18, multiple layers of artificial carriers 2 are arranged circumferentially around the isothermal module 16 at intervals. These artificial carriers 2 form the core structure for the attachment and growth of peripheral organisms. Preferably, the artificial carriers 2 are made of porous materials, most preferably in a strip-like structure. Such materials have a large specific surface area and abundant porosity, which, on the one hand, provides ample attachment sites for peripheral organisms, promoting rapid microbial attachment; on the other hand, the porous structure protects the initially attached microbial community, reducing interference from the external environment.

[0047] From the perspective of mechanism of action, the advantages of porous materials are reflected in several aspects: First, they can intercept suspended organic matter in sewage, providing a continuous supply of nutrients for periphytes and ensuring the growth and proliferation of microorganisms; Second, the strip-shaped artificial carrier will sway slightly under the action of water flow, which can enhance the turbulence of the water body, improve the contact efficiency between sewage and biofilm (periphyte formation), and promote the transfer of oxygen and nutrients in the water body, accelerating microbial proliferation; Third, the gaps inside the porous material can form a microenvironment gradient of "aerobic-anoxic-anaerobic", which can adapt to the growth needs of different functional microorganisms, enrich the diversity of biofilm, further improve the amplification efficiency and functional stability of periphytes, and are not easy to demold.

[0048] Further optimization resulted in the selection of a sponge as the artificial carrier, with a pore size of 166.21 μm, a porosity of 92.73%, and a total pore area of ​​0.512 m². 2 / g.

[0049] Ideally, multiple interfaces are provided on the side wall of the shell 1 to meet different functional requirements, namely, water inlet 3, aeration inlet 6, sludge discharge outlet 7, sampling outlet 8, and water outlet 9. Each interface has a clear division of labor and works in concert: water inlet 3 is connected to an external water inlet pipe to introduce paddy field water or nutrient water containing periphyte inoculum and nutrients into the amplification chamber 18; aeration inlet 6 is connected to an external air inlet pipe to introduce air into the chamber to provide oxygen for periphyte growth; sludge discharge outlet 7 is used to periodically discharge sludge deposited at the bottom of the amplification chamber 18 to prevent sludge accumulation from affecting the aquatic environment and microbial growth; sampling outlet 8 is used to collect water and biofilm samples from the chamber during periphyte amplification for growth status monitoring; and water outlet 9 is used to discharge the water after amplification. Among them, sampling outlet 8 and water outlet 9 are interchangeable, and in order to meet the sampling needs of different amplification stages and different depths, multiple sampling outlets 8 can be set, located at different heights of the shell 1.

[0050] To achieve uniform aeration, an aeration disc 5 is installed at the bottom of the amplification chamber 18. The aeration disc 5 is connected to the aeration inlet 6. Air introduced through the aeration inlet 6 is dispersed by the aeration disc 5 and evenly discharged into the amplification chamber 18. To facilitate the arrangement of the air intake pipe, support holes 41 are provided on the support 4 for the air intake pipe to pass through, ensuring that the pipe arrangement is standardized and does not affect the stability of the device. Even better, the aeration disc 5 is provided with multiple aeration holes with a diameter controlled between 50-100 μm. This diameter allows air to be dispersed into tiny bubbles, increasing the contact area between air and water, improving oxygen dissolution efficiency, and meeting the oxygen requirements for the growth of periphytes. To further optimize the aeration effect, the aeration disc 5 is preferably positioned in the middle of the bottom of the amplification chamber 18, directly above the thermostat module 16, with a gap between the aeration disc 5 and the bottom of the amplification chamber 18 to prevent the thermostat module 16 from blocking bubble diffusion, ensuring uniform aeration in all areas of the chamber and not affecting the overall growth of periphytes.

[0051] To achieve real-time monitoring and precise control of the environmental conditions within the amplification chamber 18, a pH probe 12 and an ORP probe 14 are also installed inside the amplification chamber 18. The pH probe 12 detects the pH value of the water within the chamber, and the ORP probe 14 detects the redox potential of the water; both are key environmental parameters for the growth of peritrichous organisms. Preferably, a pH display screen 13 and an ORP display screen 15 are installed outside the housing 1. The pH display screen 13 is electrically connected to the pH probe 12 and can display the data detected by the pH probe 12 in real time; the ORP display screen 15 is electrically connected to the ORP probe 14 and can display the data signal detected by the ORP probe 14 in real time, allowing operators to intuitively understand the environmental status within the chamber.

[0052] During actual operation, operators can adjust the light intensity of the adjustable LED 10 and the air intake of the aeration inlet 6 in a timely manner based on the real-time data displayed on the pH display screen 13 and the ORP display screen 15. At the same time, the water intake speed and nutrient supply can be adjusted through the water inlet 3 to keep the reaction conditions such as pH value, oxidation-reduction potential, light, temperature, and dissolved oxygen in the amplification chamber 18 at the most suitable state for the growth and amplification of peripheral organisms. This accelerates the migration and adsorption of microorganisms in the water to the surface of the artificial carrier, significantly shortens the biofilm formation cycle, and greatly increases the biofilm biomass. This allows the artificial carrier to have a stronger biological treatment capacity in a shorter time, which helps to achieve mass production.

[0053] Example 2 This embodiment provides a periclump organism, which is obtained by culturing the periclump organism inducing amplification in paddy fields as described in Example 1. The initial sample was collected from a natural paddy field ecosystem, and Woods Hole medium was used as the culture medium. The sample was cultured in the amplification device for 5–10 days, with an optimal culture time of 7 days.

[0054] Example 3 This embodiment also provides the device for inducing the amplification of paddy field periphytes provided in Embodiments 1 and 2 above, and / or the application of periphytes in the denitrification treatment of low C / N biogas slurry wastewater.

[0055] The above descriptions are merely embodiments of the present invention, and common knowledge regarding specific structures and characteristics in the solutions is not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent.

Claims

1. A device for inducing the amplification of periphyte organisms in paddy fields, comprising a hollow amplification cavity enclosed by a shell, wherein an artificial carrier, an aeration device, a light module, and a temperature control module are arranged within the amplification cavity, characterized in that: The artificial carrier is a porous material with a pore size of 80-250 μm, a porosity of 85-95%, and a total pore area of ​​0.20-0.80 m². 2 / g.

2. The device for inducing peritrichous amplification according to claim 1, characterized in that: The artificial carrier is a sponge with a pore size of 100-170 μm, a porosity of 91-93.5%, and a total pore area of ​​0.35-0.52 m². 2 / g.

3. The device for inducing the amplification of periphytes in paddy fields according to claim 1 or 2, characterized in that: The artificial carrier is arranged in multiple layers with spacing within the amplification cavity.

4. The apparatus for inducing the amplification of periphyte organisms in paddy fields according to any one of claims 1-3, characterized in that: The aeration device includes an aeration disc with multiple aeration holes. The diameter of the aeration holes is 50-100μm, and the aeration flow rate is adjustable.

5. The apparatus for inducing the amplification of periphyte organisms in paddy fields according to any one of claims 1-4, characterized in that: The illumination module is an adjustable LED with an illumination intensity between 10,000 and 50,000 lux.

6. The device for inducing the amplification of periphytes in paddy fields according to claim 5, characterized in that: The light cycle is 12 hours of light / 12 hours of darkness.

7. The apparatus for inducing the amplification of periphyte organisms in paddy fields according to any one of claims 1-6, characterized in that: The amplification chamber is also equipped with an ORP probe and a pH probe.

8. The device for inducing the amplification of periphytes in paddy fields according to claim 7, characterized in that: A display screen is provided on the outside of the housing to receive and display monitoring data from the ORP probe and pH probe.

9. A perianth organism, obtained by culturing the perianth organism amplification device according to any one of claims 1-8 in paddy fields.

10. The device for inducing the amplification of paddy field periphytes as described in any one of claims 1-9 and / or the application of periphytes in the denitrification treatment of low C / N biogas slurry wastewater.

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