Bacillus halodurans and application thereof in promoting growth of sesame and improving salt-tolerance

Abstract

This invention discloses a strain of *Bacillus halophilus* and its application in promoting sesame growth and improving salt and alkali tolerance, belonging to the field of sesame cultivation technology. This strain of *Bacillus halophilus* is... Halobacillus sp. OCR1-S1, with the preservation number CCTCC NO:M 20252396. Experiments showed that inoculation with the OCR1-S1 strain during the sesame germination stage under salt-alkali stress significantly promoted the growth of fresh weight, root length, and shoot length in sesame buds; inoculation with the OCR1-S1 strain during the sesame seedling stage significantly enhanced the sesame's resistance to salt-alkali stress; simultaneously, treatment of sesame roots with the OCR1-S1 strain under normal soil conditions also promoted sesame plant growth and increased phosphorus content. This invention provides a new microbial resource and a reliable solution for sesame to cope with salt-alkali stress, and is of great significance for utilizing my country's large areas of saline-alkali land to meet the needs of sesame industry expansion.

Description

Technical Field

[0001] This invention relates to the field of sesame cultivation technology, specifically to a strain of Bacillus spp. and its application in promoting sesame growth and improving sesame's salt and alkali tolerance. Background Technology

[0002] Soil salinization is a major challenge facing global agricultural production and the ecological environment. my country has a vast area of ​​saline-alkali land, totaling approximately 100 million hectares, ranking third in the world. It is widely distributed in the arid and semi-arid inland regions of Northeast, Northwest, and North China, as well as the eastern coastal areas. Salinity stress severely inhibits crop seed germination and plant growth and development through mechanisms such as reducing soil water potential, causing ion toxicity, disrupting nutrient balance, and interfering with cellular physiological metabolism. This leads to a significant decline in yield and quality, and even crop failure, severely restricting the sustainable use of land resources.

[0003] Sesame, as an important high-quality oilseed crop, has consistently ranked first in the world in domestic consumption, but its self-sufficiency rate is only around 30%, highlighting a significant supply-demand imbalance. Given the increasingly scarce arable land resources, expanding sesame cultivation on existing fertile land risks competing with staple food production and is unsustainable. Therefore, effectively developing and utilizing saline-alkali land resources to expand sesame cultivation has become a strategic path to alleviate supply pressure and ensure industry security. Large areas of saline-alkali land in China possess the potential for improvement and utilization, which highly aligns with the expansion needs of the sesame industry.

[0004] However, large-scale sesame cultivation in saline-alkali areas still faces a series of technical bottlenecks. Traditional breeding methods are time-consuming and inefficient, and the relative scarcity of salt-tolerant genetic resources in sesame germplasm limits the selection of superior salt-tolerant varieties. While genetic engineering technology has potential, practical problems remain, such as an imperfect genetic transformation system, complex multi-gene regulatory mechanisms, and immature biosafety assessments, making it difficult to achieve breakthroughs in application in the short term.

[0005] Research has discovered a special type of salt-tolerant microbial community in saline-alkali environments. This community enhances plant resilience and promotes growth through mechanisms such as promoting nutrient absorption, regulating ion balance, and producing growth-promoting substances. Exploring and utilizing these functional microbial resources provides a new direction for the biological improvement and ecological adaptation of saline-alkali land. This approach not only assists plants in adapting to saline-alkali stress but also directly serves the needs of supporting planting techniques for salt-tolerant varieties, possessing significant scientific research value and broad application prospects. Summary of the Invention

[0006] In view of this, the present invention provides a strain of Bacillus spp. and its application in promoting sesame growth and improving sesame's salt and alkali tolerance, aiming to solve the salt and alkali stress problem faced by large-scale sesame cultivation in saline-alkali areas.

[0007] The specific technical solution of the present invention is as follows: In a first aspect, the present invention provides a strain of *Bacillus halophilus*. Halobacillus sp. OCR1-S1 was deposited at the China Center for Type Culture Collection on October 30, 2025, with accession number CCTCC NO: M 20252396.

[0008] The OCR1-S1 strain was isolated from the rhizosphere soil of sesame in a saline-alkali region. It can survive in heavily saline-alkali environments (such as saline-alkali environments with a NaCl content of 10% and a pH of 10) and has the function of dissolving inorganic phosphorus (such as Ca3(PO4)2). Based on 16SRNA sequence homology analysis, it was identified as belonging to the genus *Haloxybacterium*. Halobacillus bacteria of the sp.

[0009] Secondly, the present invention provides a microbial inoculant comprising Bacillus halosarcina OCR1-S1 and / or its fermentation products. It is understood that the fermentation products may be a fermentation mixture of Bacillus halosarcina OCR1-S1, a fermentation broth filtrate, a fermentation broth extract, or a pure extract of one of its active substances, etc.

[0010] In some embodiments of the present invention, the preparation method of the microbial agent is as follows: after activating and culturing Bacillus halophilus OCR1-S1 on LB solid medium for 2-3 days, a single colony is picked and inoculated into LB liquid medium, cultured at 30°C and 220 r / min, the obtained bacterial culture is centrifuged, and the bacterial resuspended is collected.

[0011] Thirdly, the present invention provides the application of Bacillus halophilus OCR1-S1 and microbial agents containing Bacillus halophilus OCR1-S1 in improving the salt and alkali tolerance of sesame.

[0012] Preferably, in the above applications, sesame seeds are treated with Bacillus halophilus OCR1-S1 or its microbial inoculant to improve the salt and alkali tolerance of sesame during germination. Examples of the present invention show that, under salt and alkali stress, treatment of sesame seeds with Bacillus halophilus OCR1-S1 significantly increases the fresh weight, root length, and shoot length of the resulting sesame seedlings.

[0013] Preferably, in the above applications, sesame plants are treated with root irrigation using Bacillus halophilus OCR1-S1 or its microbial inoculant to improve the salt and alkali tolerance of sesame plants. Embodiments of the present invention show that under salt and alkali stress, root irrigation treatment of sesame plants with Bacillus halophilus OCR1-S1 significantly increases sesame biomass, decreases leaf electrical conductivity, increases water content, and improves leaf phosphorus and potassium levels. + The content increased significantly, while Na + The content decreased significantly, Na + / K + The ratio decreased.

[0014] Preferably, in the above applications, Bacillus halophilus OCR1-S1 is first prepared into OD. 600nm A bacterial suspension with a value of 0.2 was then used to treat sesame seeds or sesame roots.

[0015] Fourthly, the present invention provides the application of Bacillus halosa OCR1-S1 or microbial agents containing Bacillus halosa OCR1-S1 in promoting sesame growth, specifically: inoculating sesame with Bacillus halosa OCR1-S1 at least once using the root irrigation method.

[0016] Some embodiments of the present invention show that even under no salt-alkali stress, root irrigation treatment of sesame plants with Bacillus halophilus OCR1-S1 or its microbial inoculant can significantly increase the fresh weight and leaf area of ​​sesame plants, indicating that Bacillus halophilus OCR1-S1 can promote sesame growth.

[0017] Fifthly, the present invention provides the application of Bacillus halosa OCR1-S1 or microbial agents containing Bacillus halosa OCR1-S1 in increasing the phosphorus content of sesame, specifically: inoculating sesame with Bacillus halosa OCR1-S1 at least once using the root irrigation method.

[0018] Bacillus halophilus OCR1-S1 has the ability to decompose inorganic phosphorus, which can activate and utilize insoluble inorganic phosphorus, thereby promoting the absorption of phosphorus by sesame and thus significantly increasing the phosphorus content in sesame plants.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a strain of Bacillus spp., OCR1-S1, which can effectively alleviate the effects of salt-alkali stress on sesame seed germination and plant growth. Experiments show that inoculation with OCR1-S1 under salt-alkali stress significantly increases root length, shoot length, and fresh weight of sesame seeds 7 days after germination. It also increases sesame plant biomass, leaf water content, and decreases leaf electrical conductivity and leaf Na+. + Content decreased, K + Increased content and Na + / K + The ratio decreased. Furthermore, under normal soil conditions, root irrigation with OCR1-S1 also promoted sesame growth, manifested as increased leaf area, increased above-ground fresh weight, and increased phosphorus content in leaves. This invention provides a new microbial resource and a reliable solution for sesame to cope with saline-alkali stress, and is of great significance for utilizing large areas of saline-alkali land to meet the needs of sesame industry expansion.

[0020] Biological Preservation Information: Preservation institution: China Center for Type Culture Collection (CCTCC); Location of collection: Wuhan University, Wuhan, China; Deposit date: October 30, 2025; Accession number: CCTCC NO: M 20252396; Classification and nomenclature: Bacillus halophilus Halobacillus sp. OCR1-S1. Attached Figure Description

[0021] To more clearly illustrate the technical solution of the present invention, the accompanying drawings used in the present invention will be briefly described below. Obviously, the drawings described below are merely some embodiments of the present invention, and those skilled in the art can obtain other drawings based on these drawings without any creative effort.

[0022] Figure 1 The image shows the detection results of Bacillus halophilus OCR1-S1 growing well in Gibbons modified medium (pH 10 and NaCl content 10%) in Example 1.

[0023] Figure 2 This is a graph showing the detection results of phosphate-solubilizing zones formed by Bacillus halophilus OCR1-S1 in NBRIP solid medium in Example 1.

[0024] Figure 3 This is a comparison of the growth status of sesame seeds 7 days after germination, treated with Bacillus halophilus OCR1-S1 inoculant under water and saline-alkali conditions, as shown in Example 3.

[0025] Figure 4 The graph shows the statistical results of sesame seedling fresh weight (A), root length (B), and shoot length (C) 7 days after germination following treatment with Bacillus halophilus OCR1-S1 in water and saline-alkali treatments in Example 3. The letters are used to indicate whether there are significant differences between groups.

[0026] Figure 5 This is a diagram showing the growth status of sesame plants after 30 days of continuous salt and alkali stress under the treatment of Bacillus halophilus OCR1-S1 inoculum in Example 4.

[0027] Figure 6 The aboveground and underground biomass (AB) of sesame plants after 30 days of continuous salt-alkali stress under Bacillus halophilus OCR1-S1 inoculum treatment in Example 4 are: leaf water content (C), leaf electrical conductivity (D), leaf P (E), and Na. + (F), K + (G) content and Na + / K + Statistical results of the ratio (H); where This indicates that p < 0.05. This means p < 0.01.

[0028] Figure 7 This is a diagram showing the growth status of sesame plants after 21 days of treatment with Bacillus halophilus OCR1-S1 inoculant in Example 5.

[0029] Figure 8 This is a graph showing the statistical results of the aboveground biomass (A), leaf area (B), and leaf phosphorus content (C) of sesame plants after 21 days of treatment with Bacillus halophilus OCR1-S1 inoculum in Example 5. Detailed Implementation

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used herein is for the purpose of describing particular implementations only and is not intended to limit the invention; the terms “comprising” and “having”, and any variations thereof, in the specification and claims of this invention are intended to cover non-exclusive inclusion.

[0031] spp. of Haloxylon ammodendron ( Halobacillus sp.) is a group of Gram-positive, spore-forming, moderately halophilic bacteria defined by Spring et al. in 1996, with its type species being […]. Halobacillus litoralis There are currently no reports on the application of this genus of bacteria in sesame. This invention isolated and screened a strain of *Bacillus halophilus* from the rhizosphere soil of sesame in saline-alkali areas. Halobacillus sp. OCR1-S1 is resistant to heavy salt and alkali conditions and has the function of dissolving inorganic phosphorus.

[0032] Some embodiments of the present invention use a bacterial suspension of Bacillus halophilus OCR1-S1 to treat sesame seeds, which can effectively improve the fresh weight, root length and shoot length of sesame seedlings obtained under salt and alkali stress, indicating that Bacillus halophilus OCR1-S1 can improve the salt and alkali tolerance of sesame during germination.

[0033] In other embodiments of the present invention, root irrigation with a bacterial suspension of Bacillus halophilus OCR1-S1 can effectively improve the growth performance of sesame seedlings under salt-alkali stress. Specifically, this is manifested in: a significant increase in sesame biomass, a decrease in leaf electrical conductivity, an increase in water content, and improvements in leaf phosphorus and potassium content. + The content increased significantly, while Na + The content decreased significantly, Na + / K + The decrease in the ratio indicates that Bacillus halophilus OCR1-S1 can improve the salt and alkali tolerance of sesame seedlings.

[0034] In other embodiments of the present invention, sesame plants in normal soil conditions were treated with a bacterial suspension of Bacillus halophilus OCR1-S1. It was found that even without salt stress, Bacillus halophilus OCR1-S1 could still significantly promote sesame growth (manifested as a significant increase in fresh weight and leaf area), while effectively increasing the phosphorus content of the plants.

[0035] The following are some specific embodiments. It should be noted that the embodiments described below are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. Where specific techniques or conditions are not specified in the embodiments, they shall be performed in accordance with the techniques or conditions described in the literature in this field or according to the product instructions. Reagents or instruments used, unless otherwise specified, are all conventional products that can be obtained commercially.

[0036] In the quantitative experiments in the following examples, three replicate experiments were set up, and the average value of the results was taken.

[0037] The specific culture media used in the following examples and their preparation methods are as follows: LB liquid medium: Dissolve 10 g of tryptone, 5 g of yeast extract and 5 g of sodium chloride in 1 L of distilled water, sterilize at 121℃ for 15 min, and use after cooling; LB solid medium: Dissolve 10 g tryptone, 5 g yeast extract, 5 g sodium chloride and 15 g agar in 1 L distilled water, sterilize at 121℃ for 15 min, cool to 50-60℃, pour the medium into petri dishes and let it cool naturally. Gibbons modified medium: Dissolve 5 g casein, 3 g sodium citrate, 10 g yeast extract, 2 g KCl, 5 g peptone, 2 g MgSO4·7H2O, 15 g agar, and a certain amount of NaCl in 1 L of distilled water, and adjust the pH with 1 mmol / L NaOH solution as needed; the following 6 different NaCl concentrations and pH values ​​were set: 5% NaCl, pH=8.5, 8% NaCl, pH=8.5, 10% NaCl, pH=8.5, 5% NaCl, pH=10, 8% NaCl, pH=10, and 10% NaCl, pH=10; sterilize at 121℃ for 15 min, cool to 50-60℃, pour the medium into petri dishes, and allow to cool naturally; NBRIP solid medium: Weigh 10 g glucose, 5 g Ca3(PO4)2, 0.25 g MgCl2, 0.25 g MgSO4·7H2O, 0.2 g KCl, 0.1 g (NH4)2SO4 and 15 g agar, add 1 L distilled water, sterilize at 121℃ for 15 min, cool to 50-60℃, pour the medium into petri dishes and let it cool naturally.

[0038] The sesame seeds used in the following examples are from the National Oil Crop Germplasm Resource Mid-term Bank, variety number Zhongzhi 75, unified number ZZM7540, and the seed coat color is white.

[0039] Example 1 This example provides Bacillus halosa ( Halobacillus The experimental data related to the separation and identification of sp.) OCR1-S1 are as follows: (1) Isolation of bacteria OCR1-S1.

[0040] Rhizosphere soil samples were collected from healthy sesame plants grown in saline-alkali soil in Dongying, Shandong Province. One g of soil sample was added to 10 ml of sterile phosphate buffer, stirred thoroughly, and allowed to stand. 100 μL of the supernatant was taken and further diluted 500 times with sterile phosphate buffer. 100 μL of the suspension was then spread onto six different concentrations of Gibbons modified agar medium and incubated at 30°C for 3-5 days. Single colonies from the Gibbons modified agar medium were picked and purified at least three times. One selected bacterial strain was named OCR1-S1.

[0041] Single colonies of OCR1-S1 were inoculated into LB liquid medium and cultured at 30°C and 220 r / min for 10 h to obtain bacterial culture. The bacterial culture was mixed with 60% (v / v) glycerol at a ratio of 1:1 (v:v) and stored at -80°C.

[0042] (2) Functional identification of bacterial OCR1-S1.

[0043] OCR1-S1 bacterial culture stored at -80℃ and a negative control strain (this strain lacks high salt and alkali tolerance and phosphate-solubilizing function) were activated on LB solid medium. After culturing for 2-3 days, a single colony was picked from the LB solid medium and inoculated into 10 mL of LB liquid medium. The culture was incubated at 30℃ and 220 rpm / min for 10 h. The resulting bacterial culture was then analyzed. 600nm Adjust the value to 0.02. Take 10 uL of the obtained cell culture medium and drop it onto Gibbons modified solid medium (10% NaCl, pH=10) and NBRIP solid medium respectively, and incubate at 30℃ in the dark for 3-5 days.

[0044] The culture results showed that the OCR1-S1 strain grew well in Gibbons modified solid medium, forming smooth plaques, while the negative control strain showed almost no growth. Figure 1 In NBRIP medium, a transparent phosphate-solubilizing zone forms around OCR1-S1 plaques, while no obvious plaques are observed around negative plaques. Figure 2The above results demonstrate that OCR1-S1 can survive in a saline-alkali environment with a NaCl content of 10% and a pH of 10, and also has the function of phosphorus solubilization.

[0045] (3) Molecular identification of bacterial OCR1-S1.

[0046] Homology analysis was performed using 16SRNA sequences. The 16SRNA of bacterial OCR1-S1 was compared with sequences in NCBI, and the results showed that bacterial OCR1-S1 is homologous to *Bacillus halophilus*. Halobacillus marinus It has the highest homology, reaching 99.92%.

[0047] Based on the above analysis results, the bacteria OCR1-S1 isolated and purified in this case was identified as belonging to the genus *Salmonella* (…). Halobacillus sp.).

[0048] Example 2 This example provides a solution containing Bacillus halophilus ( Halobacillus The preparation of the microbial agent (sp.) OCR1-S1 includes the following steps: Take the OCR1-S1 bacterial culture stored at -80℃ from Example 1 and activate it on LB solid medium. After culturing for 2-3 days, pick a single colony from the LB solid medium and inoculate it into 100 mL of LB liquid medium (250 mL sterile Erlenmeyer flask). Incubate at 30℃ and 220 r / min for 10 h to obtain a bacterial culture. Centrifuge the bacterial culture at 3000 rpm for 10 min. After centrifugation, remove the supernatant and collect the bacterial cells at the bottom.

[0049] Resuspend the bacteria at the bottom in sterile water or saline solution to obtain a bacterial resuspension, and then set the OD value accordingly. 600nm The value was adjusted to 0.2. The bacterial suspension prepared with sterile water is the prepared OCR1-S1 bacterial agent; the bacterial suspension prepared with saline-alkali solution is the OCR1-S1 saline-alkali treatment solution. The saline-alkali solution is a mixture of NaCl and NaHCO3, with a molar ratio of NaCl to NaHCO3 of 9:1, a total molar concentration of 50 mmol / L, and a pH of 8.2 ± 0.1.

[0050] Example 3 This example tested the role of Bacillus halosarcina OCR1-S1 in improving salt and alkali tolerance during sesame germination. The specific experiment is as follows: Take plump sesame seeds with good germination rate, treat them with 75% alcohol solution for 30 seconds, wash them three times with sterile water, then treat them with 1% (v / v) sodium hypochlorite aqueous solution for 5 minutes, and finally wash them three times with sterile water.

[0051] Four treatment groups were set up: CK (treatment solution is sterile water), OCR1-S1 (treatment solution is OCR1-S1 bacterial agent in implementation case 2), saline-alkali treatment (treatment solution is saline-alkali solution in implementation case 2), and saline-alkali + OCR1-S1 treatment (treatment solution is OCR1-S1 saline-alkali treatment solution in implementation case 2).

[0052] Two layers of sterile filter paper were placed in the germination box, and 10 ml of the corresponding treatment solution was added. 50 sesame seeds that had undergone the above pretreatment were sown in each germination box. The culture conditions were: 30℃, dark culture for 2 days, and then light culture for 5 days. The light conditions were 14 h light / 10 h dark and the light intensity was 7000 Lx.

[0053] After cultivation, observe the growth status of sesame seeds; record the fresh weight, root length and bud length of the seedlings, and randomly count 20 seedlings from each germination box.

[0054] The results are as follows Figure 3-4 As shown, under salt-alkali stress, treatment with OCR1-S1 inoculant increased the fresh weight, root length, and shoot length of sesame seedlings by 35.4%, 40.5%, and 70.6%, respectively, compared to the control. This demonstrates that OCR1-S1 can significantly improve the salt-alkali tolerance of sesame seedlings during germination.

[0055] Example 4 This example tested the role of Bacillus halophilus OCR1-S1 in improving the salt and alkali tolerance of sesame seedlings. The specific experiment is as follows: Take plump sesame seeds with good germination rate, treat them with 75% alcohol solution for 30 seconds, wash them 3 times with sterile water, treat them with 1% (v / v) sodium hypochlorite aqueous solution for 5 minutes, and finally wash them 3 times with sterile water.

[0056] The pretreated sesame seeds were sown in a seedling substrate and placed in a plant growth chamber at 30℃, with a 14-hour light / 10-hour dark cycle and a light intensity of 7000 Lx. Thinning was performed after the seedlings developed their first pair of true leaves, leaving one healthy plant per pot. Once the first pair of true leaves were fully expanded, OD seeds were inoculated using the root drenching method. 600nm The Bacillus halophilus OCR1-S1 inoculum (prepared in Example 2) with a value of 0.2 was used to inoculate each seedling with 5 mL of the inoculum; the control group was inoculated with 5 mL of sterile water per seedling. Inoculation was performed once every 2 weeks, for a total of 2 inoculations.

[0057] Three days after inoculation, a saline-alkali treatment was performed using a mixture of NaCl and NaHCO3 in a molar ratio of 9:1, with a total molar concentration of 150 mmol / L and a pH of 8.2 ± 0.1. This treatment was repeated every two weeks for a total of two treatments.

[0058] After one month of continuous treatment, the growth status of sesame was recorded, the fresh weight of the above-ground and underground parts of the plant was counted, and the leaves were collected to determine the leaf conductivity, leaf water content, leaf phosphorus content, and leaf sodium content. + Content, K + Content and Na + / K + ratio.

[0059] Test results as follows Figure 5-6 As shown: Under salt-alkali stress, after applying OCR1-S1 inoculant, the fresh weight of the above-ground and underground parts of sesame plants increased by 49.0% and 88.3%, respectively. Figure 5 and Figure 6 AB), the blade conductivity decreased by 88.1% ( Figure 6 D) Leaf water content increased by 4.3% ( Figure 6 C), Leaf P and K + The content increased by 25.9% and 26.5% respectively. Figure 6 E and Figure 6 G), while Na + The content decreased by 23.2% ( Figure 6 F), Na + / K + The ratio decreased by 39.4% ( Figure 6 H). The above results demonstrate that OCR1-S1 can enhance the salt and alkali tolerance of sesame seedlings.

[0060] Example 5 This example tested the effect of Bacillus halosarcina OCR1-S1 in promoting sesame growth. The specific experiment is as follows: Take plump sesame seeds with good germination rate, treat them with 75% alcohol solution for 30 seconds, wash them 3 times with sterile water, treat them with 1% (v / v) sodium hypochlorite aqueous solution for 5 minutes, and finally wash them 3 times with sterile water.

[0061] The pretreated sesame seeds were sown in a seedling substrate and placed in a plant growth chamber at 30℃ with a 14-hour light / 10-hour dark cycle and a light intensity of 7000 Lx. Thinning was performed after the seedlings developed their first pair of true leaves, leaving one healthy plant per pot. Once the first pair of true leaves were fully expanded, OD seeds were inoculated using the root drenching method. 600nm The Bacillus halophilus OCR1-S1 inoculum (prepared in Example 2) with a value of 0.2 was used to inoculate each seedling with 5 mL of the inoculum; the control group was inoculated with 5 mL of sterile water per seedling. Inoculation was performed once a week for a total of 3 weeks.

[0062] Three weeks after treatment, plant growth was observed, and the fresh weight of the above-ground parts of the plant, the leaf area of ​​the whole plant (excluding the first pair of true leaves) and the phosphorus content of the leaves were recorded.

[0063] The results are as follows Figure 7-8 As shown: After inoculation with OCR1-S1 inoculant, the fresh weight of sesame plants ( Figure 8 A) Total leaf area of ​​the plant ( Figure 8 B) and phosphorus content in leaves ( Figure 8 C) Compared with the control treatment, the growth rates were increased by 88.2%, 45.4%, and 18.1%, respectively. These results demonstrate that OCR1-S1 can significantly promote sesame growth.

[0064] In summary, the Bacillus halophilus OCR1-S1 provided by this invention can not only effectively improve the salt and alkali tolerance of sesame at various stages, but also promote sesame growth and increase phosphorus content, providing a new microbial resource and a reliable solution for sesame to cope with salt and alkali stress.

[0065] It should be noted that the present invention is not limited to the above-described embodiments. The above embodiments are merely examples, and any embodiments that have the same structure and perform the same effects as the technical concept within the scope of the present invention are included within the scope of the present invention. Furthermore, various modifications that can be conceived by those skilled in the art to the embodiments, and other ways of constructing by combining some of the constituent elements of the embodiments, without departing from the spirit of the present invention, are also included within the scope of the present invention.

Claims

1. A strain of Bacillus halophilus OCR1-S1, with accession number CCTCC NO: M 20252396.

2. A microbial inoculant, characterized in that, It includes Bacillus halophilus OCR1-S1 as described in claim 1.

3. The application of Bacillus halophilus OCR1-S1 as described in claim 1 or the microbial agent as described in claim 2 in improving the salt and alkali tolerance of sesame.

4. The application according to claim 3, characterized in that, Sesame seeds were treated with the aforementioned Bacillus halosa OCR1-S1 or microbial inoculants to improve the salt and alkali tolerance of sesame during the germination period.

5. The application according to claim 3, characterized in that, The sesame plants were treated with root irrigation using the Bacillus halophilus OCR1-S1 or microbial inoculants to improve their salt and alkali tolerance.

6. The application according to claim 3, characterized in that, The OD of the microorganism 600nm The value is 0.

2.

7. The application of Bacillus halophilus OCR1-S1 as described in claim 1 or the microbial agent as described in claim 2 in promoting sesame growth.

8. The application according to claim 7, characterized in that, Sesame seeds were inoculated with Bacillus halosa OCR1-S1 at least once using the root irrigation method.

9. The application of Bacillus halophilus OCR1-S1 as described in claim 1 or the microbial agent as described in claim 2 in increasing the phosphorus content of sesame seeds.

10. The application according to claim 9, characterized in that, Sesame seeds were inoculated with Bacillus halosa OCR1-S1 at least once using the root irrigation method.

Images