Test strip for detecting thiophanate-methyl in vegetables and fruits and application thereof

The rapid test strip for methylthiophanate prepared using colloidal gold immunochromatography technology solves the problems of insufficient sensitivity and complex operation of existing detection methods, and realizes rapid and low-cost detection of methylthiophanate residues, which is suitable for large-scale sample testing in grassroots units.

CN122361794APending Publication Date: 2026-07-10BEIJING KWINBON BIOTECH
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Patent Information

Application Number
CN202610155769.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-04
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing methods for detecting thiophanate-methyl suffer from insufficient sensitivity, complex operation, high cost, and long detection time, making it difficult to meet the needs of grassroots units for large-scale rapid testing.

Method used

Using colloidal gold immunochromatography, rapid test strips are prepared using thiophanate-methyl monoclonal antibody-colloidal gold labeling and thiophanate-methyl hapten-carrier protein conjugate, achieving rapid and sensitive detection through antigen-antibody reaction.

Benefits of technology

It achieves high sensitivity, low cost, and rapid detection of thiophanate-methyl residues, is suitable for large-scale sample testing in grassroots units, is simple to operate, provides intuitive results, and has a wide range of applications.

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Abstract

This invention discloses a test strip for detecting thiophanate-methyl and its application. The test strip includes a sample absorption pad (1), a conjugate release pad (2), a reaction membrane (3), an absorbent pad (4), and a base plate (7). The reaction membrane has a detection line (5) coated with a thiophanate-methyl hapten-carrier protein conjugate and a control line (6) coated with goat anti-mouse anti-antibody. The conjugate release pad (2) is coated with a thiophanate-methyl monoclonal antibody-colloidal gold label. This invention also provides a method for detecting thiophanate-methyl residues in vegetables and fruits using the above-mentioned thiophanate-methyl test strip. The test strip provided by this invention has the characteristics of simple operation, high sensitivity, fast detection speed, and low cost, and is suitable for screening large numbers of samples and on-site monitoring.
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