An immunochromatographic test card, a detection method and application for widening the quantitative detection range

By combining the sandwich method and the competitive method in the immunochromatographic detection card, the detection range is expanded, the hook effect problem of high concentration samples is solved, and high sensitivity and accurate detection are achieved over a wide concentration range, making it suitable for multi-item joint detection.

CN122361799APending Publication Date: 2026-07-10WUXI BIOHERMES BIO & MEDICAL TECH CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUXI BIOHERMES BIO & MEDICAL TECH CO LTD
Filing Date
2026-04-24
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing immunochromatographic detection techniques exhibit a hook effect in high-concentration samples, resulting in a narrow detection range and poor accuracy. Existing methods either reduce sensitivity or increase costs, and the signal gradient is poor near the critical concentration.

Method used

The test card design combines sandwich and competitive methods. By setting capture antibody detection lines, antigen detection lines, and control lines on the nitrocellulose membrane and coating different markers on the conjugate pad, the optimal detection mode is automatically selected based on the curve algorithms of each item, thus expanding the detection range.

Benefits of technology

It achieves good linearity over a wide concentration range, avoids the hook effect, ensures detection accuracy and sensitivity, is suitable for multi-item joint detection, and reduces the need for dilution of high-concentration samples.

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Abstract

This invention discloses an immunochromatographic assay card, detection method, and application for broadening the quantitative detection range. The immunochromatographic assay card for broadening the quantitative detection range includes: a sample pad, a conjugation pad, a nitrocellulose membrane pad, and absorbent paper sequentially connected on a base plate; the nitrocellulose membrane pad includes a detection zone and a control zone; the detection zone is provided with a capture antibody detection line and an antigen detection line, and the control zone is provided with a control line; the conjugation pad is coated with a first label and a second label, the first label including a labeled antibody that binds to the target analyte, and the second label including a label that specifically binds to the capture reagent coated on the control line. This invention, by combining the sandwich method with a competitive method, effectively broadens the linear detection range, avoids the hook effect, and improves the accuracy and sensitivity of the detection, making it particularly suitable for quantitative detection of items with a wide concentration range.
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