Oyster functional component stable type extraction equipment based on high-speed air circulation technology

Abstract

The application discloses a functional component stable extraction equipment for oysters based on high-speed air circulation technology, which comprises high-speed air circulation pretreatment units, extraction units, solid-liquid separation units and vacuum low-temperature concentration units which are sequentially sealed and communicated, and further comprises an online detection unit and a PLC controller electrically connected with each unit, the high-speed air circulation pretreatment unit comprises a pretreatment cavity, a hot air circulation assembly arranged in the sealed pretreatment cavity, a material bearing and overturning assembly and a multidimensional sensing assembly, the extraction unit comprises an extraction cavity and an ultrasonic-assisted extraction assembly, and the online monitoring unit is connected with the pretreatment unit and the extraction unit respectively. Through the cooperative linkage of the high-speed air circulation pretreatment unit and the extraction unit, the application realizes the synergistic improvement of high retention rate of curcumin, high dissolution rate of active ingredients and high stability of batches, simultaneously reduces energy consumption and operation difficulty, adapts to the industrialized continuous production demand, and has significant technical progress and practical value.

Description

Technical Field

[0001] This invention relates to the field of oyster functional component extraction equipment technology, and more particularly to a stable oyster functional component extraction equipment based on high-speed air circulation technology. Background Technology

[0002] The global incidence of inflammatory diseases continues to rise, and dietary intervention, due to its safety and high compliance, has become a core direction for adjuvant treatment of inflammatory diseases. Curcumin, as the core active ingredient of turmeric, can target and regulate the nuclear factor NF-κB inflammatory signaling pathway and has excellent antioxidant activity, making it an ideal functional factor for dietary intervention. Oysters, as a high-quality aquatic resource, are rich in oyster bioactive peptides, taurine, zinc, and other components that can produce anti-inflammatory synergistic effects with curcumin. At the same time, the polar groups of oyster proteins can form a protective system through intermolecular hydrogen bonds, significantly improving the stability of curcumin.

[0003] However, the β-diketone structure and aromatic ring conjugated system in curcumin molecules are highly sensitive to heat and oxygen, and are prone to hydrolysis and oxidative degradation during processing and extraction. In existing technologies, the retention rate of curcumin after traditional processing is generally below 50%, severely restricting the development of its functional products. Currently, for the processing and extraction of the oyster-turmeric complex system, existing extraction equipment operates with isolated pretreatment and extraction units, lacking a synergistic linkage mechanism. This prevents dynamic parameter adaptation based on the real-time state of the material, resulting in low curcumin retention, unbalanced extraction efficiency, and poor batch stability. This fails to meet the industrial requirements for stable extraction of heat-sensitive components from the oyster-turmeric complex system. Therefore, a dedicated extraction device with synergistic linkage capabilities between the two units is urgently needed to overcome these technical bottlenecks. Summary of the Invention

[0004] The purpose of this invention is to address the aforementioned problems by providing a stable extraction device for oyster functional components based on high-speed air circulation technology. Through the synergistic linkage between the high-speed air circulation pretreatment unit and the extraction unit, the core technical bottleneck of the prior art is fundamentally solved, achieving a synergistic improvement in high retention rate of curcumin, high dissolution rate of active ingredients, and high batch stability. At the same time, it reduces energy consumption and operational difficulty, adapts to the needs of continuous industrial production, and has significant technological advancements and practical value.

[0005] To achieve the above-mentioned objectives, the technical solution adopted by this invention is as follows: According to one aspect of the present invention, a stable extraction device for oyster functional components based on high-speed air circulation technology is provided, comprising a high-speed air circulation pretreatment unit, an extraction unit, a solid-liquid separation unit and a vacuum low-temperature concentration unit that are sequentially sealed and connected, and further comprising an online detection unit and a PLC controller electrically connected to each unit; The high-speed air circulation pretreatment unit includes a sealed pretreatment cavity and a hot air circulation component, a material carrying and flipping component, and a multi-dimensional sensing component disposed in the sealed pretreatment cavity. The hot air circulation component is used to provide circulating hot air to the pretreatment cavity, the material carrying and flipping component is used to carry and flip the material, and the multi-dimensional sensing component is used to collect environmental and material data in the pretreatment cavity. The extraction unit includes an extraction chamber and an ultrasonic-assisted extraction component. The inlet of the extraction chamber is connected to the outlet of the pretreatment chamber through a sealed spiral feeding mechanism. The ultrasonic-assisted extraction component is fixedly installed in the extraction chamber. The feed inlet of the solid-liquid separation unit is connected to the discharge outlet of the extraction chamber, and the feed inlet of the vacuum low-temperature concentration unit is connected to the clear liquid outlet of the solid-liquid separation unit. The online monitoring unit includes an online near-infrared spectroscopy module and an online detection component. The online near-infrared spectroscopy module is fixedly installed in the pretreatment chamber, and the online detection component is connected to the extraction chamber. The PLC controller is electrically connected to the hot air circulation component, the material carrying and turning component, the multi-dimensional sensing component, the ultrasonic-assisted extraction component, the online near-infrared spectroscopy module, and the online detection component, respectively.

[0006] Preferably, the hot air circulation assembly includes a centrifugal fan, an electric heating tube, and an air guide plate arranged in a ring along the inner wall of the pretreatment chamber. The electric heating tube is fixedly installed at the air outlet of the centrifugal fan. An annular air guide channel is formed between the air guide plate and the inner wall of the pretreatment chamber, and a radiant heat area is formed on the inner side of the air guide plate. The annular air guide channel and the radiant heat area are interconnected.

[0007] Preferably, the hot air circulation assembly further includes a return air duct disposed on the side wall of the sealed pretreatment chamber, the air inlet of the return air duct being connected to the pretreatment chamber, and the air outlet of the return air duct being connected to the air inlet of the centrifugal fan.

[0008] Preferably, the material carrying and flipping assembly includes a hollow material tray, a drive motor, and a rotating spindle. The rotating spindle is horizontally inserted inside the sealed pretreatment cavity. The drive motor is fixedly connected to the rotating spindle, and the hollow material tray is fixedly mounted on the rotating spindle.

[0009] Preferably, the multi-dimensional sensing component includes a temperature sensor, a wind speed sensor, and an infrared temperature probe disposed within the cavity, wherein the temperature sensor, the wind speed sensor, and the infrared temperature probe are electrically connected to the PLC controller.

[0010] Preferably, the ultrasonic-assisted extraction assembly includes multiple sets of frequency-adjustable ultrasonic transducers, which are evenly distributed at the bottom of the extraction chamber, and the ultrasonic transducers are electrically connected to the PLC controller.

[0011] Preferably, the online near-infrared spectroscopy module includes a light emitting unit, a light collecting unit, and a photoelectric conversion unit. The light emitting unit is used to emit near-infrared light to the material, the light collecting unit is used to collect diffuse reflected light from the material, and the photoelectric conversion unit is electrically connected to the PLC controller to convert the collected light signal into an electrical signal.

[0012] Preferably, the online detection component includes a sampling pump and a high-performance liquid chromatography (HPLC) detection module. The inlet of the sampling pump is connected to the extraction chamber, and the outlet is connected to the HPLC detection module. The HPLC detection module is electrically connected to the PLC controller and is used to detect the concentrations of curcumin and oyster active peptides in the extract in real time.

[0013] Preferably, the solid-liquid separation unit includes a tubular centrifuge, which is electrically connected to the PLC controller.

[0014] Preferably, the vacuum low-temperature concentration unit includes a scraped vacuum concentrator, the inlet of which is connected to the clear liquid outlet of the solid-liquid separation unit.

[0015] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. This invention constructs a closed-loop linkage control system for pretreatment and extraction by using an online near-infrared spectroscopy module and an online detection component. The online near-infrared spectroscopy module captures the curcumin retention rate and material state during the pretreatment stage and feeds it back to the PLC controller in real time. This links with the closed-loop low-temperature extraction unit to adjust the temperature control and nitrogen purging parameters in advance, preventing curcumin from hydrolyzing or oxidizing and degrading due to overheating. The system also monitors the curcumin concentration in the extract in real time, accurately determining the extraction endpoint and avoiding structural damage caused by over-extraction. Simultaneously, the pretreatment parameters are finely adjusted in reverse, forming a two-way linkage protection system that improves the curcumin retention rate and effectively protects its β-diketone structure and aromatic ring conjugated system, providing support for the development of functional products.

[0016] 2. The online near-infrared spectroscopy module and online detection component of this invention monitor key indicators of the solid material in the pretreatment stage and the liquid extract in the extraction stage in real time. Both sets of data are synchronously transmitted to the PLC. The PLC automatically adjusts the parameters of the two units in response to differences in the state of materials from different batches, effectively avoiding quality differences caused by material fluctuations between batches and reducing the relative deviation of curcumin retention rates between batches. Simultaneously, the two detection components achieve automated online detection, reducing manual intervention. Combined with the coordinated control of the two units, this meets the industrial continuous production requirements for the stable extraction of thermosensitive components from the oyster-curcumin composite system. Attached Figure Description

[0017] Figure 1 This is a block diagram showing the connections between the various units of the present invention; Figure 2 This is a schematic diagram of the structure of the high-speed air exchange pretreatment unit of the present invention; Figure 3 This is a schematic diagram of the extraction unit of the present invention; Figure 4 This is a schematic diagram of the parameter response under the optimal process conditions of the present invention; Figure 5 This is a schematic diagram illustrating the effect of different amounts of turmeric powder added on the curcumin retention rate according to the present invention; Figure 6 This is the UV spectrum of oysters with 3% turmeric powder added according to the present invention; Figure 7 The near-infrared spectrum of oyster with 3% turmeric powder added according to the present invention.

[0018] In the attached diagram: 1. Sealed pretreatment chamber; 2. Centrifugal fan; 3. Electric heating element; 4. Air guide plate; 5. Hollow material tray; 6. Drive motor; 7. Rotary spindle; 8. Extraction chamber; 9. Inert gas protection interface; 10. Solvent inlet; 11. Ultrasonic transducer; 12. Sampling pump; 13. High-performance liquid chromatography detection module. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and preferred embodiments. However, it should be noted that many details listed in the specification are merely to provide the reader with a thorough understanding of one or more aspects of the invention, and these aspects of the invention can be achieved even without these specific details.

[0020] Please see Figures 1 to 3 This invention provides a stable extraction device for oyster functional components based on high-speed air circulation technology, the technical solution of which is as follows: The oyster functional component stabilization extraction equipment based on high-speed air circulation technology includes a high-speed air circulation pretreatment unit, an extraction unit, a solid-liquid separation unit, and a vacuum low-temperature concentration unit that are sealed and connected in sequence. It also includes an online detection unit and a PLC controller that is electrically connected to each unit.

[0021] The high-speed air circulation pretreatment unit includes a sealed pretreatment chamber 1, a hot air circulation assembly, a material carrying and turning assembly, and a multi-dimensional sensing assembly. The hot air circulation assembly includes a centrifugal fan 2, an electric heating element 3, and a guide plate 4 arranged annularly along the inner wall of the chamber, all mounted on the sealed pretreatment chamber 1. The electric heating element 3 is fixedly installed at the outlet of the centrifugal fan 2. An annular airflow channel is formed between the guide plate 4 and the inner wall of the pretreatment chamber, and a radiant heat zone is formed inside the guide plate 4. The annular airflow channel and the radiant heat zone are interconnected. The outlet of the centrifugal fan 2 generates stable hot air through the electric heating element 3, and the return air formed through the annular airflow channel ensures more uniform heating within the radiant heat zone. A return air channel is provided on the side wall of the sealed pretreatment chamber 1. The inlet of the return air channel connects to the bottom of the chamber, and the outlet connects to the inlet of the centrifugal fan 2, forming a closed-loop hot air circulation circuit. A removable dust filter element is installed inside the return air channel to intercept material powder and prevent dust contamination during hot air circulation.

[0022] The material-carrying and flipping assembly includes a perforated material tray 5, a drive motor 6, and a rotating spindle 7. The rotating spindle 7 is horizontally inserted inside the sealed pretreatment chamber 1. One end of the rotating spindle 7 is fixedly connected to the output end of the drive motor 6, which is fixedly mounted on the pretreatment chamber. The perforated material tray 5 is fixed to the rotating spindle 7, and the drive motor 6 drives the perforated material tray 5 to flip at a uniform speed of 5-15 r / min via the rotating spindle 7. The perforated material tray 5 has a hole diameter of 3 mm, and the inner wall of the tray is coated with a food-grade polytetrafluoroethylene non-stick coating. The tray includes a tray body and a tray cover. The tray cover is fastened to the tray body for easy material loading and unloading.

[0023] The multi-dimensional sensing components include a temperature sensor, a wind speed sensor, and an infrared temperature probe housed within the cavity. These sensors are electrically connected to the PLC controller. The temperature and wind speed sensors are fixedly mounted within the pretreatment cavity to collect real-time data on the hot air temperature and wind speed. The infrared temperature probe is fixedly mounted on the top of the pretreatment cavity, directly opposite the tray, to collect real-time data on the material surface temperature. All data collected by the temperature, wind speed, and infrared temperature probes is transmitted to the PLC controller in real time.

[0024] The inlet of the extraction unit is connected to the bottom outlet of the sealed pretreatment chamber 1 via a sealed screw feeding mechanism, achieving closed-loop conveying of the pretreated material and preventing contact with air. The extraction unit includes an extraction chamber 8, a jacketed low-temperature constant temperature module, an ultrasonic-assisted extraction component, an inert gas protection interface 9, and a solvent inlet 10. The jacketed low-temperature constant temperature module is wrapped around the outer wall of the extraction chamber 8 and uses a water bath constant temperature circulation to maintain a constant temperature of 35-45℃ inside the extraction chamber 8. The ultrasonic-assisted extraction component includes four sets of frequency-adjustable ultrasonic transducers 11, evenly distributed at the bottom of the extraction chamber 8. The frequency of the ultrasonic transducers 11 is adjustable from 20-40kHz, and the power of a single transducer is 50-200W. The inert gas protection interface 9 is connected to both the sealed pretreatment chamber 1 and the extraction chamber 8, allowing for the continuous filling with high-purity nitrogen to isolate oxygen and reduce the risk of oxidation of active ingredients.

[0025] The online monitoring unit includes an online near-infrared spectroscopy module and an online detection component. The online near-infrared spectroscopy module comprises a light emission unit, a light acquisition unit, and a photoelectric conversion unit. The light emission unit includes a near-infrared LED chip array, emitting light with wavelengths of 900–1700 nm. This array is fixedly positioned within the pretreatment cavity, directly emitting near-infrared detection light onto the material. The light acquisition unit includes a miniature focusing lens array for collecting diffusely reflected light from the material. The photoelectric conversion unit includes an InGaAs photodetector chip, which directly converts the collected light signal into an analog electrical signal. After internal filtering and amplification, the signal is converted into a digital spectral signal and output to the PLC controller.

[0026] The online detection component includes a sampling pump 12 and a high-performance liquid chromatography (HPLC) detection module 13. The inlet end of the sampling pump 12 extends into the extraction chamber 8, and the outlet end is connected to the HPLC detection module 13. It can detect the concentration of curcumin and oyster active peptides in the extract in real time and transmit the detection data to the PLC controller. When the curcumin concentration reaches the preset threshold, the extraction is automatically terminated.

[0027] The solid-liquid separation unit is a tubular centrifuge with a high separation factor, enabling continuous solid-liquid separation of the extract. Its inlet is connected to the bottom outlet of the extraction chamber 8. The vacuum low-temperature concentration unit is a scraped-plate vacuum concentrator, whose inlet is connected to the clear liquid outlet of the solid-liquid separation unit.

[0028] The PLC controller is electrically connected to the hot air circulation assembly, material handling and turning assembly, multi-dimensional sensing assembly, jacketed low-temperature constant temperature module, ultrasonic-assisted extraction assembly, solid-liquid separation unit, and vacuum low-temperature concentration unit. It has a built-in library of preset process parameters for the oyster-turmeric composite system, enabling multi-parameter collaborative closed-loop control of the entire process from pretreatment to extraction, separation, and concentration. The process parameter library contains preset optimal process parameter sets: sealed pretreatment chamber 1 temperature 180℃, air velocity 2m / s, material turning speed 10r / min, pretreatment time 6min; extraction chamber 8 temperature 45℃, ultrasonic frequency 28kHz, ultrasonic power 400W, extraction time 40min.

[0029] The extraction steps for the oyster-turmeric complex functional components are as follows: S1. Material pretreatment: Take fresh oysters, remove the shells and clean the meat. Rinse with sterile water at 4℃ and homogenize at 10000r / min for 2min to prepare oyster meat paste. Mix the oyster meat paste with turmeric powder at a mass ratio of 40% oyster meat paste and 3% turmeric powder to obtain a mixture. Spread the mixture evenly in the hollow material tray 5 with a thickness of 1cm. Close the sealing door of the pretreatment chamber 1 and fill it with high-purity nitrogen through the inert gas protection interface 9 to replace the air in the chamber. The residual oxygen content is ≤0.5%.

[0030] S2. High-speed air circulation stabilization pretreatment: The optimal process parameter set is called through the PLC controller, starting the hot air circulation component and the material carrying and turning component, controlling the chamber temperature at 180℃, hot air velocity at 2m / s, and material turning speed at 10r / min, with a pretreatment time of 6min; after pretreatment, it is naturally cooled to below 40℃. The parameter response surface under the optimal process conditions is as follows: Figure 4 As shown.

[0031] The effect of varying the proportion of turmeric powder added on the optimal high-speed air circulation technology on curcumin retention rate was analyzed. Results are as follows: Figure 5 As shown, with the gradual increase of turmeric powder addition, the overall curcumin retention rate reached over 60% with minimal fluctuations between groups, exhibiting good stability. This indicates that the high-speed air circulation technology, through uniform hot air circulation, can achieve uniform cooking of oyster cakes without accelerating the hydrolysis, oxidation, and other degradation reactions of curcumin due to excessively high temperatures. The 6-minute processing time balances the adequacy of oyster cake cooking with the cumulative degradation of curcumin, avoiding insufficient cooking due to too short a time or accelerated curcumin degradation due to too long a time. It also avoids localized overheating or prolonged heating that could lead to curcumin degradation, providing stable and favorable conditions for curcumin production. The processing technology of 180℃, 6 minutes, and 2 m / s speed was verified to maintain a high curcumin retention level in turmeric-powdered oysters.

[0032] S3. Closed-loop low-temperature extraction: After pretreatment, the material is directly fed into the extraction chamber 8 through a sealed screw feeder. An 80% methanol aqueous solution containing 0.1% phosphoric acid is added at a material-to-liquid ratio of 1:10 (g / mL). Nitrogen gas is introduced into the extraction chamber 8 through the inert gas protection interface 9 for protection. The jacketed low-temperature constant temperature module is activated to control the temperature of the extraction chamber 8 to a constant 45℃. The ultrasonic-assisted extraction component is activated, with the ultrasonic frequency set to 28kHz and the ultrasonic power to 400W. An intermittent working mode is adopted: ultrasonic operation for 2 seconds, pause for 3 seconds, and extraction time for 40 minutes. During the extraction process, the curcumin concentration is monitored in real time by an online detection component. When the concentration tends to stabilize, the extraction is terminated.

[0033] S4. Solid-liquid separation and concentration: After extraction, the extract is sent to a tubular centrifuge and centrifuged at 3500 r / min for 10 min to remove residue. The clear liquid is sent to a scraped vacuum concentrator and concentrated at low temperature at 40℃ and 0.09 MPa vacuum to obtain oyster-turmeric complex functional component concentrate.

[0034] Comparative Example 1 A control experiment was conducted using a traditional hot reflux extraction device. Using the same material ratio and raw materials as in this embodiment, the process parameters of the traditional hot reflux extraction equipment are as follows: the material is not pretreated by high-speed air circulation, and the extraction solvent is added directly at the same material-liquid ratio. The hot reflux extraction temperature is 80°C and the extraction time is 2 hours. After extraction, the material is centrifuged and concentrated under the same conditions to obtain the control sample.

[0035] Comparative Example 2 A control experiment was conducted using an oven pretreatment and a low-temperature cold soaking device. Using the same material ratio and raw materials as in this embodiment, the pretreatment was carried out in a traditional oven at 180°C for 6 minutes without turning, hot air circulation, or nitrogen protection. After pretreatment, the material was subjected to low-temperature cold maceration extraction at 45°C for 120 minutes, with the remaining conditions consistent with the embodiment, to obtain the control sample.

[0036] Example 3 Sample Performance Testing The concentrates obtained from Examples 1, 2, and 3 were subjected to performance testing. The test indicators included curcumin retention rate, DPPH radical scavenging rate, ABTS radical scavenging rate, and anti-inflammatory activity (NO release inhibition rate). The test results are shown in the table below: The test results show that the samples prepared using the equipment described in this invention have significantly better curcumin retention rate, antioxidant activity, and anti-inflammatory activity than those prepared using traditional equipment. The curcumin retention rate is 156% higher than that of traditional hot reflux equipment and 58.6% higher than that of oven pretreatment and cold soaking equipment, which fully demonstrates the stabilizing and protective effect of the equipment of this invention on heat-sensitive functional components and its excellent extraction performance.

[0037] Ultraviolet full-wavelength scanning is a key method for characterizing the state of matter, structural integrity, and matrix interference. This invention explores the effect of high-speed air circulation technology on the structure of curcumin through comparative analysis of three sets of spectra. Figure 6 As shown, the CK group only showed two weak absorption peaks at 200-220 nm (n-σ transition of starch hydroxyl groups) and 270-290 nm (π→π transition of tyrosine and tryptophan residues in oyster meat protein). There were no obvious absorption peaks in the curcumin characteristic absorption region at around 420 nm, and the absorbance in the 300-400 nm range was close to the baseline (absorbance <0.05). This result confirms that the matrix itself does not produce interfering absorption in the curcumin characteristic absorption region. The untreated group exhibited a sharp, symmetrical, and strong characteristic absorption peak at 420 nm (absorbance value of 1.432), with no peak splitting or tailing, consistent with the typical spectral characteristics of the curcumin molecular conjugation system (β-diketone structure conjugated with aromatic rings). Simultaneously, matrix absorption peaks at 200-220 nm and 270-290 nm remained, but their absorbance values ​​(1.923 and 0.752, respectively) were significantly lower than the 420 nm curcumin characteristic peak, indicating no masking or superposition interference with the curcumin characteristic peak. Combining peak integrity and absorption intensity, it can be concluded that with 3% curcumin powder added, curcumin is uniformly dispersed in the oyster paste, and its initial structure remains intact. The 3% addition group still maintained a clear characteristic absorption peak of curcumin at 420 nm, with no obvious peak distortion, indicating that the high-speed air circulation technology did not cause serious structural damage to curcumin. Compared with the untreated group, the absorbance value at 420 nm decreased by about 18.7%, but the absorbance values ​​of the matrix absorption peaks at 200-220 nm and 270-290 nm did not change significantly (p>0.05), proving that the high-speed air circulation technology only caused a small amount of curcumin molecule degradation, but the core conjugated structure necessary for curcumin to exert its biological activity was preserved as a whole, and the destructive effect of the process on the curcumin structure was weak.

[0038] By comparing the infrared spectra of the experimental group, the matrix blank group, and the untreated group, and combining the molecular structure of curcumin (containing phenolic hydroxyl groups, aromatic rings, β-diketones, ether bonds, and side-chain alkyl groups) with the characteristics of the oyster matrix, the changes in the molecular structure of the system and the mechanism of action of curcumin were clarified. Figure 7As shown, the matrix blank group exhibited weak absorptions at 1530 cm⁻¹ (protein amide II NH bending vibration) and 1150 cm⁻¹ (carbohydrate CO bond stretching vibration). Both the untreated group and the 3% turmeric powder group showed significant curcumin characteristic absorption peaks at 3500 cm⁻¹ (free OH stretching vibration), 1710 cm⁻¹ (C=O stretching vibration), 1580 cm⁻¹ (aromatic ring skeleton vibration), 1280 cm⁻¹ (COC ether bond stretching vibration), and 830 cm⁻¹ (para-substituted benzene ring CH bending vibration). Heating is key to driving the synergistic effect between curcumin and the matrix. Compared to the untreated group, the hydrogen bonds between curcumin molecules in the 3% turmeric powder group dissociated, reaching a peak at 3250 cm⁻¹. - The double peak of the associated OH peak disappeared at ¹, and the transmittance increased from 56.3% to 74.8%. The free phenolic hydroxyl groups after dissociation formed new weak hydrogen bonds with the polar groups (such as amide NH) and carbohydrate hydroxyl groups exposed after protein denaturation in the oyster cake matrix; at the same time, the β-diketone structure of curcumin underwent keto-enol tautomerism

[21] , 1710 cm - The transmittance of the C=O peak at ¹ increased from 44.8% to 62.2%, and the reduced free carbonyl groups prevented excessive cross-linking with protein amino groups; in addition, the vibrational coupling of aromatic ring substituents was weakened, and the transmittance at 1580 cm⁻¹ was reduced. - The splitting peak at ¹ became shallower, and the transmittance increased from 45.9% to 65.3% at 2920 cm⁻¹. - ¹Side chain alkyl groups are combined with oyster cake 2850 cm - ¹ The saturated CH bonds of the lipids at this point undergo concerted vibrations, causing the double-peaked peak to transform into a diffuse peak, while the core framework of curcumin (ether bond, aromatic ring C=C, para-substitution structure) remains intact. In the untreated group, because the matrix was not activated by heating, curcumin exists in an independent molecular state, and at 3250 cm⁻¹... - The peak at ¹ shows a strong hydrogen bond association double peak, at 1710 cm⁻¹. - ¹High free carbonyl peak and 1580 cm⁻¹ - ¹ Strong aromatic ring coupling splitting peak; after heating, the free phenolic hydroxyl groups of curcumin in the 3% curcumin powder group form stable hydrogen bonds with the amide NH and carbohydrate hydroxyl groups of oyster cake protein, and the aromatic ring and the polar side chain of the protein produce conjugation effect. Moreover, no new impurity peaks are generated in the blank group, indicating that the heating process ensures the structural stability and matrix compatibility of curcumin, providing support for the functionalization of oyster.

[0039] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A stable extraction device for oyster functional components based on high-speed air circulation technology, characterized in that, It includes a high-speed air circulation pretreatment unit, an extraction unit, a solid-liquid separation unit, and a vacuum low-temperature concentration unit that are sequentially sealed and connected. It also includes an online detection unit and a PLC controller that is electrically connected to each unit. The high-speed air circulation pretreatment unit includes a sealed pretreatment cavity and a hot air circulation component, a material carrying and flipping component, and a multi-dimensional sensing component disposed in the sealed pretreatment cavity. The hot air circulation component is used to provide circulating hot air to the pretreatment cavity, the material carrying and flipping component is used to carry and flip the material, and the multi-dimensional sensing component is used to collect environmental and material data in the pretreatment cavity. The extraction unit includes an extraction chamber and an ultrasonic-assisted extraction component. The inlet of the extraction chamber is connected to the outlet of the pretreatment chamber through a sealed spiral feeding mechanism. The ultrasonic-assisted extraction component is fixedly installed in the extraction chamber. The feed inlet of the solid-liquid separation unit is connected to the discharge outlet of the extraction chamber, and the feed inlet of the vacuum low-temperature concentration unit is connected to the clear liquid outlet of the solid-liquid separation unit. The online monitoring unit includes an online near-infrared spectroscopy module and an online detection component. The online near-infrared spectroscopy module is fixedly installed in the pretreatment chamber, and the online detection component is connected to the extraction chamber. The PLC controller is electrically connected to the hot air circulation component, the material carrying and turning component, the multi-dimensional sensing component, the ultrasonic-assisted extraction component, the online near-infrared spectroscopy module, and the online detection component, respectively.

2. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The hot air circulation assembly includes a centrifugal fan, an electric heating tube, and an air guide plate arranged in a ring along the inner wall of the pretreatment chamber. The electric heating tube is fixedly installed at the air outlet of the centrifugal fan. An annular air guide channel is formed between the air guide plate and the inner wall of the pretreatment chamber, and a radiant heat area is formed on the inner side of the air guide plate. The annular air guide channel and the radiant heat area are interconnected.

3. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 2, characterized in that: The hot air circulation assembly also includes a return air duct disposed on the side wall of the sealed pretreatment chamber. The air inlet of the return air duct is connected to the pretreatment chamber, and the air outlet of the return air duct is connected to the air inlet of the centrifugal fan.

4. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The material carrying and flipping assembly includes a hollow material tray, a drive motor, and a rotating spindle. The rotating spindle is horizontally inserted inside the sealed pretreatment cavity. The drive motor is fixedly connected to the rotating spindle, and the hollow material tray is fixedly mounted on the rotating spindle.

5. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The multi-dimensional sensing component includes a temperature sensor, a wind speed sensor, and an infrared temperature probe disposed within the cavity. The temperature sensor, wind speed sensor, and infrared temperature probe are electrically connected to the PLC controller.

6. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The ultrasonic-assisted extraction assembly includes multiple sets of frequency-adjustable ultrasonic transducers, which are evenly distributed at the bottom of the extraction chamber. The ultrasonic transducers are electrically connected to the PLC controller.

7. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The online near-infrared spectroscopy module includes a light emitting unit, a light collecting unit, and a photoelectric conversion unit. The light emitting unit is used to emit near-infrared light to the material, the light collecting unit is used to collect diffuse reflected light from the material, and the photoelectric conversion unit is electrically connected to the PLC controller to convert the collected light signal into an electrical signal.

8. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The online detection component includes a sampling pump and a high-performance liquid chromatography (HPLC) detection module. The inlet of the sampling pump is connected to the extraction chamber, and the outlet is connected to the HPLC detection module. The HPLC detection module is electrically connected to the PLC controller and is used to detect the concentration of curcumin and oyster active peptides in the extract in real time.

9. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The solid-liquid separation unit includes a tubular centrifuge, which is electrically connected to the PLC controller.

10. The oyster functional component stabilization extraction device based on high-speed air circulation technology according to claim 1, characterized in that: The vacuum low-temperature concentration unit includes a scraped vacuum concentrator, the inlet of which is connected to the clear liquid outlet of the solid-liquid separation unit.

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