Preparation method and application of enzyme-controlled nutrient microspheres with built-in glucose delivery system
By preparing enzyme-controlled nutrient microspheres with built-in glucose delivery systems, the problems of uncontrollable glucose release and osmotic pressure imbalance have been solved, achieving safe energy supply and improved cell survival rate, and has broad application prospects in tissue regeneration.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- QILU INST OF TECH
- Filing Date
- 2026-04-25
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies suffer from problems such as uncontrollable glucose release, easy osmotic pressure imbalance, inactivation of exogenous enzymes inside microspheres, performance limitations of natural polysaccharides and synthetic polymers, residual organic solvents, and high costs.
Enzyme-controlled nutrient microspheres with an integrated glucose delivery system were prepared by dissolving a hydrogel carrier, glucosyl amylase (AMG), and a cross-linking agent in an aqueous starch solution to form an aqueous phase. The aqueous phase was then mixed with an emulsifier and solvent, heated and stirred, cross-linked under ultraviolet light, and freeze-dried. Starch molecules formed a low-osmotic-pressure active reservoir in the GelMA network, and glucose was slowly diffused after enzymatic hydrolysis.
It achieves safe energy supply, reduces the risk of osmotic pressure imbalance, improves the performance limitations of natural polysaccharides and synthetic polymers, provides controllable degradation and cell adhesion, and improves cell survival rate and the stability of glucose release.
Smart Images
Figure CN122398735A_ABST