Bacillus licheniformis-derived peptide-2 and its application in the prevention and control of root-knot nematode disease

By optimizing the nematicide-2 derived from Bacillus licheniformis, the problems of scarce root-knot nematode resistance resources and pollution from chemical control have been solved, providing an efficient and green control method and improving the control effect of root-knot nematodes.

CN122404498APending Publication Date: 2026-07-17HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2026-05-19
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Root-knot nematodes cause serious damage and there is a lack of resistant resources. Chemical control leads to pesticide residues and resistance, making it difficult to meet the needs of green agriculture.

Method used

The nematicidal peptide Peptide-2 derived from Bacillus licheniformis was optimized using bioinformatics technology. By introducing methionine at the amino terminus and adjusting the number of positively charged residues and hydrophobic characteristics, it was optimized into a highly lethal nematicidal peptide.

Benefits of technology

Peptide-2 exhibits high lethality against root-knot nematodes and a high corrected mortality rate, meeting the needs of green agriculture for the development of biocontrol agents and the creation of new germplasm resources for root-knot nematode-resistant crops, thus overcoming the shortcomings of chemical control.

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Abstract

本发明公开一种地衣芽孢杆菌源杀线肽Peptide‑2及其在根结线虫病害防控中的应用,所述杀线肽Peptide‑2的氨基酸序列如SEQ ID NO.1所示。本发明还提供了一种防治根结线虫的药剂,所述药剂包括上述的杀线肽Peptide‑2。本发明的杀线肽Peptide‑2来源明确、序列短、易于化学合成或异源表达,可应用于根结线虫绿色生防制剂的开发以及抗根结线虫作物新种质资源创制,丰富了根结线虫抗性基因资源,有助于克服现有化学杀线剂的农药残留、抗药性、生态污染等缺陷,符合绿色农业发展需求。
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