An alcohol dehydrogenase mutant and its application in preparing trans-4-propylcyclohexanol
By directionally mutagenesis of Rhodococcus alcohol dehydrogenase, the alcohol dehydrogenase mutant F43V/Y294G was formed, which solved the problem of insufficient stereoselectivity in the preparation of trans-4-propylcyclohexanol, realized a highly efficient and mild catalytic reaction, and improved production efficiency and economy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- NANJING UNIV
- Filing Date
- 2026-04-20
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for the preparation of trans-4-propylcyclohexanol suffer from insufficient stereoselectivity, resulting in harsh reaction conditions, the use of hazardous reagents, the generation of environmental pollutants, and poor overall atom economy, making it difficult to achieve efficient and green synthesis.
By directionally mutagenesis of Rhodococcus alcohol dehydrogenase, specifically by replacing amino acids at positions 43 and 294 of the amino acid sequence with specific amino acids, an alcohol dehydrogenase mutant F43V/Y294G was formed, which is used to catalyze the efficient stereoselective reduction of 4-propylcyclohexanone.
The alcohol dehydrogenase mutant F43V/Y294G significantly improves catalytic activity and stereoselectivity, with mild reaction conditions and reduced difficulty in product separation and purification, thereby enhancing the production efficiency and economic benefits of high-value drugs and liquid crystal materials.
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Figure CN122405573A_ABST