Eucalyptus globulus drought-related transcription factor gene egdreb2a and its encoding protein and application

By cloning and applying the EgDREB2A gene, a drought-resistant transcription factor for Eucalyptus globulus, constructing a recombinant vector and introducing it into Eucalyptus globulus cells, the problem of low breeding efficiency of Eucalyptus globulus was solved, enabling the rapid cultivation of drought-resistant Eucalyptus globulus varieties and improving their growth performance and breeding efficiency in arid environments.

CN122405640APending Publication Date: 2026-07-17SOUTHWEST FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST FORESTRY UNIVERSITY
Filing Date
2026-03-11
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Eucalyptus globulus grows under limited conditions in arid environments. Existing breeding methods are inefficient and lack key drought-resistant gene resources, making it difficult to quickly cultivate highly drought-resistant varieties.

Method used

The EgDREB2A gene, a drought-resistant transcription factor associated with Eucalyptus globulus, was cloned. The drought resistance of Eucalyptus globulus and other plants was improved through genetic engineering. A recombinant vector was constructed using the EgDREB2A gene and its encoded protein. The vector was introduced into Eucalyptus globulus cells via Agrobacterium-mediated transformation. After screening and culturing, plants with significantly better drought resistance than non-transgenic plants were obtained.

Benefits of technology

The goal is to rapidly cultivate new drought-resistant Eucalyptus varieties, improve survival rate and growth, reduce water consumption, mitigate economic losses caused by drought stress, and provide genetic resources and technical references for improving the drought resistance of other plants.

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Abstract

本发明属于植物基因工程技术领域,具体是一种蓝桉抗旱相关转录因子基因EgDREB2A及其编码蛋白与应用。本发明从蓝桉(Eucalyptus globulus)中克隆获得EgDREB2A基因,其核苷酸序列如SEQ ID NO:1所示,编码蛋白的氨基酸序列如SEQ ID NO:2所示,包含AP2 DNA结合结构域(第58‑117位氨基酸)。该基因受干旱胁迫强烈诱导表达,24h达峰值(18.6倍)。本发明构建了含EgDREB2A的重组表达载体,通过农杆菌介导法转化蓝桉,获得转基因植株。转基因植株在干旱胁迫下表现出显著增强的抗旱性:叶片相对含水量提高60%,脯氨酸含量提高3.4倍,电解质渗漏率降低50%,且下游抗旱基因Rd29A、P5CS、LEA显著上调表达。亚细胞定位显示EgDREB2A蛋白定位于细胞核,符合转录因子特征。本发明为培育抗旱蓝桉新品种提供了重要基因资源和有效方法。
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