Construction of a deletion-rescue insect baculovirus protein expression system and its application

By constructing insect cell lines that stably express GP64 and baculovirus genomes with the GP64 gene knocked out, the problem of GP64 protein interfering with the expression of exogenous proteins was solved, achieving efficient and stable recombinant protein production suitable for industrial applications.

CN122405741APending Publication Date: 2026-07-17CHENGDU NANOMICROGEN BIOTECH CO LTD +1
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Patent Information

Application Number
CN202511846438.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-09
Publication Date
2026-07-17

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Abstract

本发明涉及生物工程技术领域，特别涉及一种缺失‑拯救型昆虫杆状病毒蛋白表达系统的构建及其应用。本发明提供了一种新的昆虫杆状病毒表达平台，以突破传统BEVS在外源蛋白表达中的瓶颈。该平台由稳定表达杆状病毒膜融合蛋白GP64的昆虫细胞株（Sf9‑GP64）与敲除gp64基因的重组杆状病毒载体（Δgp64‑Bacmid）组成。转染Sf9‑GP64细胞后，病毒可被拯救并扩增；拯救的病毒可感染Sf9或Hi5细胞，实现高效外源蛋白表达而不再产生病毒扩增。该系统显著提高蛋白表达量、可溶性和稳定性，降低宿主及病毒源杂质，简化纯化流程。由于感染后病毒不增殖、不裂解细胞，该平台在重组蛋白、VLP及疫苗抗原的高纯度制备中具备高产、稳定、安全的产业化应用价值。
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Citation Information

Patent Citations

  • Recombinant baculovirus genome and its transformation method, application and method for expressing foreign protein

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