Construction method and application of high-rhamnolipid-producing engineered pseudomonas aeruginosa
By overexpressing key genes in Pseudomonas aeruginosa PAO1, a recombinant engineered strain was constructed, which solved the problem of low rhamnolipin production and achieved a significant increase in rhamnolipin production and efficient separation and purification of downstream products.
CN122405780APending Publication Date: 2026-07-17TIANJIN UNIV OF SCI & TECH
View PDF 0 Cites 0 Cited by
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TIANJIN UNIV OF SCI & TECH
- Filing Date
- 2026-04-09
- Publication Date
- 2026-07-17
Smart Images
Figure CN122405780A_ABST
Abstract
本发明公开了一种高产鼠李糖脂的工程铜绿假单胞菌构建方法及应用。该方法通过在铜绿假单胞菌中过表达参与鼠李糖生物合成的关键基因rmlA、rmlB、rmlC、rmlD、algC，以及鼠李糖脂生物合成途径中的核心基因rhlA、rhlB、rhlC和rhlG，构建高效合成鼠李糖脂的工程菌株。通过该方法，有效克服了鼠李糖供应不足和鼠李糖脂合成效率低下的问题，显著提高了铜绿假单胞菌的鼠李糖脂产量。本发明还涉及利用该方法构建的工程菌株，以及该工程菌株在实际生产中的应用。
Need to check novelty before this filing date? Find Prior Art