Serum core fucosylated antithrombin-iii protein detection kit

By using magnetic bead-coupled antibody and lectin LCA, the core fucosylated antithrombin-III protein can be directly detected from serum samples, solving the problem of complex protein purification in existing technologies, simplifying detection and improving accuracy, and providing a new tool for the early diagnosis of liver diseases and tumors.

CN122410047APending Publication Date: 2026-07-17AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Patent Information

Application Number
CN202610546877.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-23
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing mass spectrometry and lectin chip technologies require complex protein purification steps when detecting glycosylated antithrombin-III protein, and cannot directly use serum samples, which increases the complexity and difficulty of detection and limits their application in clinical testing.

Method used

By using magnetically bead-coupled anti-ATⅢ protein antibody and lentil lectin LCA, core fucosylated antithrombin-III protein can be directly detected from serum samples via colorimetric reaction and ELISA reader, simplifying the detection process and improving detection accuracy.

Benefits of technology

It enables precise measurement of the level of core fucosylated antithrombin-III protein in serum, simplifies the detection procedure, reduces equipment and reagent costs, facilitates implementation in clinical settings, and provides a time window for early disease diagnosis.

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Abstract

本发明涉及一种血清核心岩藻糖基化抗凝血酶‑Ⅲ蛋白检测试剂盒,所述试剂盒包括标准抗原、捕获物和检测物;所述标准抗原为来源于健康个体血清的ATⅢ蛋白;所述捕获物为磁珠偶联的抗ATⅢ蛋白抗体;所述检测物为经生物素标记的能够识别核心岩藻糖的小扁豆凝集素(LCA)。相较于现有技术方案,本发明无需对血清样本进行复杂的蛋白纯化步骤,允许直接使用微量血清样本进行分析,从而显著简化了检测步骤;所需设备和试剂易于获取,使得检测过程既便捷又经济,便于在临床环境中实施;由于ATⅢ蛋白的糖基化状态在肝脏疾病和癌症发展过程中发生改变,这种高效检测手段为疾病的早期诊断和治疗提供了宝贵的时间窗口。
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