Immunohistochemical staining kit

Abstract

The utility model relates to a staining kit, in particular to an immunohistochemical staining kit. The kit comprises a storage box, a clamping plate is arranged in the storage box, a plurality of clamping grooves are evenly formed in the clamping plate, a kit body is arranged in the clamping grooves, a plurality of placing grooves are evenly formed in the kit body around the circle center, reagent bags are arranged in the placing grooves, and different reagents are stored in the reagent bags; a sliding groove is formed in the inner wall of the top end of the kit close to the placing groove, and a cutting assembly is slidably connected into the sliding groove and used for cutting the reagent capsule. According to the kit storage box, a combined structure of the clamping grooves and the kits is designed, and the kits are placed in the clamping grooves, so that the kits are orderly arranged in the storage box and are convenient to manage and take, the efficiency of an experiment preparation stage is favorably improved, and disorder and loss of the kits are reduced; the reagent bag body can be stably placed in the placing groove, stability and safety of the reagent in the storage process are guaranteed, meanwhile, the reagent bag body can rapidly release the reagent into the kit after being cut through the arrangement of the liquid inlet, automatic feeding of the reagent is achieved, and complexity and errors of manual operation are avoided.

Description

Technical Field

[0001] The utility model relates to a staining kit, in particular to an immunohistochemical staining kit. Background Art

[0002] Staining reagents are commonly used tools in biomedical research and clinical diagnosis. They utilize the specificity of antigen-antibody reactions to form antigen-antibody complexes by binding labeled antibodies to target antigens in tissues, and then display the presence and distribution of target proteins through color developers.

[0003] In clinical experiments, frozen sections need to be placed in the test kit, and then multiple reagents such as primary antibodies, secondary antibodies, blocking agents, color developers, buffer solutions, fixatives, etc. are added one by one. These reagents often need to be added manually by experimenters, and the equipment needs to be cleaned and disinfected after addition. If multiple frozen sections need to be tested, the progress and efficiency of the test will be affected. Experimenters should spend their time and energy on analyzing data results.

[0004] Therefore, it is urgent to provide an immunohistochemical staining kit to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide an immunohistochemical staining kit to solve the problem that in the existing immunohistochemical staining test, many reagents need to be added manually in sequence and the equipment needs to be cleaned, resulting in low efficiency.

[0006] The purpose of the utility model is achieved through the following technical solutions: an immunohistochemical staining kit, comprising a storage box, a splint is provided in the storage box, a plurality of slots are evenly opened in the splint, and the reagent kit is provided in the slots;

[0007] The reagent kit is provided with a plurality of placement grooves evenly arranged around the center of the circle, wherein reagent capsules are provided in the placement grooves, and different reagents are stored in the reagent capsules;

[0008] A sliding groove is provided on the inner wall of the top of the reagent box adjacent to the placement groove, and a cutting assembly is slidably connected in the sliding groove for cutting the reagent capsule. The reagent box is provided with a cap adapted to the reagent box, and the cutting assembly is fixedly connected to the inner wall of the top of the cap;

[0009] A guide groove adapted to the cutting assembly is also provided in the top of the reagent box.

[0010] As a further improvement of the present technical solution, a liquid inlet connected to the reagent kit is provided in the bottom end of the placement groove, so as to allow the reagent in the reagent capsule to flow into the reagent kit.

[0011] As a further improvement of the present technical solution, the cutting assembly includes a slider, the top of which is fixedly connected to the inner wall of the top of the cap, and a cutting block is fixedly connected to one side of the slider for cutting the reagent capsule.

[0012] As a further improvement of the present technical solution, the sliding block is in an L-shaped structure, and the contact surface between the cutting block and the reagent capsule is in a sharp wedge-shaped structure.

[0013] As a further improvement of the present technical solution, the guide groove is a circular ring structure and is in an open state, with the opening direction facing the inside of the reagent kit.

[0014] As a further improvement of the present technical solution, when the slider is placed at the bottom end of the slide groove, the top inner wall of the cap fits with the top of the reagent box, and at this time the horizontal end of the slider is also located in the guide groove.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. This immunohistochemical staining kit, by designing a combined structure of a card slot and a reagent kit, places the reagent kit in the card slot, so that the reagent kit is arranged in an orderly manner in the storage box, which is convenient for management and access, and is conducive to improving the efficiency of the experimental preparation stage and reducing the confusion and loss of the reagent kit; secondly, the reagent capsule can be stably placed in the placement slot, ensuring the stability and safety of the reagent during storage; at the same time, the setting of the liquid inlet enables the reagent capsule to quickly release the reagent into the reagent kit after cutting, realizing automatic reagent delivery and avoiding the tedious manual operation and errors.

[0017] 2. This immunohistochemical staining kit drives the cutting assembly to slide in the slide and guide groove by rotating the cap, and the cutting block automatically cuts the reagent capsule. This automated process greatly simplifies the reagent addition steps and improves the convenience and efficiency of experimental operations. Secondly, the cap not only serves as a sealing component of the kit to ensure the stability and safety of the reagents during the experiment, but also serves as a driving component of the cutting assembly. Automatic reagent addition can be achieved by rotating it. This design greatly saves experimental time and improves experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 This is an exploded view of the test kit of the present utility model;

[0020] Figure 3 This is a schematic structural diagram of the cutting assembly of the present utility model;

[0021] Figure 4It is a schematic diagram of the cross-sectional structure of the test kit of the present utility model.

[0022] The meaning of each number in the figure is:

[0023] 1. Storage box; 2. Clamp; 3. Reagent kit; 4. Cap; 5. Cutting assembly; 6. Placement slot; 7. Reagent capsule; 8. Guide slot; 9. Slide; 10. Liquid inlet; 51. Slider; 52. Cutting block. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0026] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referenced. Therefore, a feature identified as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of this utility model, "plurality" or "several" means two or more, unless otherwise specifically defined.

[0027] See also Figure 1-Figure 4 As shown, the purpose of this embodiment is to provide an immunohistochemical staining kit, including a storage box 1, a splint 2 is provided in the storage box 1, a plurality of slots are evenly opened in the splint 2, and a reagent kit 3 is provided in the slots.

[0028] Specifically, a plurality of placement grooves 6 are evenly arranged around the center of the circle in the reagent chamber 3. Reagent capsules 7 are arranged in the placement grooves 6. Different reagents are stored in the reagent capsules 7. It should be noted that a liquid inlet 10 connected to the reagent chamber 3 is provided at the bottom of the placement groove 6 for flowing the reagents in the reagent capsules 7 into the reagent chamber 3.

[0029] Furthermore, a slide groove 9 is provided on the inner wall of the top of the reagent box 3 adjacent to the placement groove 6, and a cutting assembly 5 is slidably connected to the slide groove 9. The slide groove 9 extends vertically, wherein the cutting assembly 5 includes a slider 51, the top of the slider 51 is fixedly connected to the inner wall of the top of the cap 4, and a cutting block 52 is fixedly connected to one side of the slider 51. It should be noted that the slider 51 is an L-shaped structure, and the contact surface of the cutting block 52 with the reagent capsule 7 is a sharp wedge-shaped structure for cutting the reagent capsule 7; the cap 4 is adapted to the reagent box 3 to close the top of the reagent box 3, and when the slider 51 is placed at the bottom of the slide groove 9, the top inner wall of the cap 4 fits with the top of the reagent box 3, and at this time the horizontal end of the slider 51 is also located in the guide groove 8;

[0030] Furthermore, a guide groove 8 adapted to the cutting assembly 5 is provided in the top of the reagent box 3 . It is worth mentioning that the guide groove 8 is a circular ring structure and is in an open state, with the opening direction facing the inside of the reagent box 3 .

[0031] The specific working principle is as follows:

[0032] During operation, several frozen sections are placed in each reagent box 3, and then the slider 51 in the cap 4 is aligned with the slide groove 9 and snapped into place. When the cap 4 completely covers the top of the reagent box 3 and the slider 51 is snapped into the bottom end of the slide groove 9, the horizontal end of the slider 51 is also in the guide groove 8. In this way, the cap 4 can be rotated to drive the L-shaped slider 51 to rotate, and the cutting block 52 fixedly connected to one side can cut the reagent capsule 7 exposed in the guide groove 8. It should be noted that the placement groove 6 and the guide groove 8 overlap, so that the reagent capsule 7 can be exposed in the guide groove 8, which is convenient for the cutting block 52 to cut the reagent capsule 7 when sliding in the guide groove 8.

[0033] It should also be noted that, depending on the direction of rotation of the slider 51, the reagent capsules 7 placed in each placement slot 6 in sequence should follow the experimental principle, that is, which one is added first, which one is added later, and how much is added to prevent implementation errors, and the amount of reagent in the reagent capsule 7 is also added as needed.

[0034] In this way, while rotating the cap 4, each reagent capsule 7 is cut, so that the reagents inside flow into the reagent box 3 through the liquid inlet 10 in the placement groove 6 in turn, act on the frozen section, and complete the test agent addition with high efficiency. In addition, the cap 4 can seal the reagent box 3, thereby improving the safety and accuracy of the experiment.

[0035] This effectively solves the problem that in existing immunohistochemical staining experiments, many reagents need to be added manually in sequence, and the equipment needs to be cleaned, which affects efficiency.

[0036] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely preferred examples of the present invention and are not intended to limit the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and improvements fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. An immunohistochemical staining kit, characterized in that: The invention comprises a storage box (1), wherein a clamping plate (2) is arranged in the storage box (1), a plurality of card slots are evenly opened in the clamping plate (2), and a reagent box (3) is arranged in the card slots; A plurality of placement grooves (6) are evenly arranged around the center of the circle in the reagent box (3), and a reagent capsule (7) is arranged in the placement groove (6), and different reagents are stored in the reagent capsule (7); A sliding groove (9) is provided on the inner wall of the top end of the reagent box (3) adjacent to the placement groove (6), and a cutting assembly (5) is slidably connected in the sliding groove (9) for cutting the reagent capsule (7). A cap (4) adapted to the reagent box (3) is provided on the reagent box (3), and the cutting assembly (5) is fixedly connected to the inner wall of the top end of the cap (4); A guide groove (8) adapted to the cutting assembly (5) is also provided in the top end of the reagent box (3).

2. The immunohistochemical staining kit according to claim 1, characterized in that: A liquid inlet (10) connected to the reagent box (3) is provided at the bottom of the placement tank (6) for allowing the reagent in the reagent capsule (7) to flow into the reagent box (3).

3. The immunohistochemical staining kit according to claim 1, wherein: The cutting assembly (5) comprises a slider (51), the top end of which is fixedly connected to the inner wall of the top end of the cap (4), and a cutting block (52) is fixedly connected to one side of the slider (51) for cutting the reagent capsule (7).

4. The immunohistochemical staining kit according to claim 3, characterized in that: The sliding block (51) is in an L-shaped structure, and the contact surface between the cutting block (52) and the reagent capsule (7) is in a sharp wedge-shaped structure.

5. The immunohistochemical staining kit according to claim 3, characterized in that: The guide groove (8) is a circular ring structure and is in an open state, with the opening direction facing the inside of the reagent box (3).

6. The immunohistochemical staining kit according to claim 3, characterized in that: When the slider (51) is placed at the bottom end of the slide groove (9), the top inner wall of the cap (4) fits with the top end of the reagent box (3), and at this time, the horizontal end of the slider (51) is also located in the guide groove (8).

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