Cell-free protein synthesis reactor

Abstract

The utility model relates to the technical field of biomedical instruments, in particular to a cell-free protein synthesis reactor which comprises an outer sleeve, a protein synthesis tube and a pop-up component, the protein synthesis tube is mounted on the inner side of the outer sleeve, the pop-up component comprises a mounting ring, an abutting plate, a driving component and a driving plate, the mounting ring is fixedly connected with the protein synthesis tube, and the abutting plate is fixedly connected with the driving component. The mounting ring is fixedly connected with the outer sleeve and located on the side, away from the outer sleeve, of the protein synthesis tube, the abutting plate is fixedly connected with the mounting ring and located on the side, away from the protein synthesis tube, of the mounting ring, the driving plate is connected with the outer sleeve through the driving component, the driving component drives the driving plate to move, and the driving plate abuts against the abutting plate, so that the protein synthesis tube and the outer sleeve are separated more stably and easily; and therefore, the use safety is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological medical instrument technical field especially relates to a cell -free protein synthesis reactor. BACKGROUND

[0002] The cell -free protein synthesis reactor can exchange the material required for protein synthesis through the material exchange window, and complete the cell -free protein synthesis. The size of the current material exchange window directly affects the exchange efficiency or the protein synthesis efficiency. The existing synthesis reactor cannot change the fixed size of the material exchange window, which is very inconvenient in the use process of cell -free protein synthesis and cannot control the exchange influencing factors.

[0003] In the existing patent technology CN219930090U cell -free protein synthesis reactor, the sealing cover is recorded, and the outer sleeve capable of containing protein synthesis reagent is also recorded. The protein synthesis tube is arranged in the outer sleeve, and the protein synthesis tube and the outer sleeve can exchange the synthesis raw materials required for protein synthesis. The cell -free protein synthesis reactor forms a stable bearing reaction structure through the outer sleeve and the protein synthesis tube capable of being inserted and matched with each other, which is convenient for assembly and separation. The synthesis reactor can change the conduction degree of the synthesis material exchange window through the adjusting cover, has adjustability, is conducive to controlling the efficiency of protein synthesis. In addition, the shrinkable pipe body structure with gradually decreasing pipe diameter at the bottom of the outer sleeve can obtain greater liquid level pressure, which is conducive to promoting reagent exchange and better promoting protein synthesis.

[0004] In actual use, the protein synthesis tube is connected to the outer sleeve tightly. After the reaction is completed, the staff needs to spend a lot of effort to take out the protein synthesis tube from the outer sleeve, which is easy to cause the rupture of the protein synthesis tube and has low safety. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a cell -free protein synthesis reactor, which solves the problem that the staff needs to spend a lot of effort to take out the protein synthesis tube from the outer sleeve after the reaction is completed, which is easy to cause the rupture of the protein synthesis tube and has low safety.

[0006] To achieve the above-mentioned purpose, the utility model provides a cell -free protein synthesis reactor, which comprises an outer sleeve, a protein synthesis tube and a pop -out assembly. The protein synthesis tube is installed on the inner side of the outer sleeve. The pop -out assembly comprises a mounting ring, an abutment plate, a driving member and a driving plate. The mounting ring is fixedly connected with the protein synthesis tube and located on the side of the protein synthesis tube away from the outer sleeve. The abutment plate is fixedly connected with the mounting ring and located on the side of the mounting ring away from the protein synthesis tube. The driving plate is connected with the outer sleeve through the driving member. The driving member drives the driving plate to move. The driving plate abuts against the abutment plate.

[0007] The driving member comprises a mounting seat, a threaded rod and a transmission element, the mounting seat is fixedly connected with the outer sleeve and located at one side of the outer sleeve close to the driving plate, the threaded rod is rotatably connected with the mounting seat and located at one side of the mounting seat close to the driving plate, and the threaded rod is connected with the driving plate, the transmission element is installed on the outer sleeve, and the transmission element drives the threaded rod to rotate.

[0008] The transmission element comprises a rotating ring and a driving gear, the rotating ring is slidably connected with the outer sleeve and located at one side of the outer sleeve close to the mounting seat, and the driving gear is fixedly connected with the threaded rod and located at one end of the threaded rod close to the rotating ring, and the driving gear is connected with the rotating ring.

[0009] The transmission element further comprises a rubber pad, the rubber pad is fixedly connected with the rotating ring and located at one side of the rotating ring away from the driving gear.

[0010] The driving member further comprises a sliding block, the sliding block is fixedly connected with the driving plate and located at one side of the driving plate close to the outer sleeve, and the sliding block is connected with the outer sleeve.

[0011] The utility model discloses a cell -free protein synthesis reactor, the mounting ring is installed on the outside of protein synthesis pipe through bolt, the abutted board is installed on the one side away from the protein synthesis pipe of mounting ring through bolt, make the outer sleeve can be located at the interlayer of abutted board and protein synthesis pipe, the driving plate is installed on the outside of outer sleeve through driving member, drives the driving plate to move upwards through driving member, thereby makes the driving plate with abutted board and abutted board drive, and move upwards in turn, and then drive protein synthesis pipe to move upwards, thereby make protein synthesis pipe move out the outer sleeve, thereby realize more stable, easy separation protein synthesis pipe and outer sleeve, thereby improved the security when using. BRIEF DESCRIPTION OF DRAWINGS

[0012] In order to more clearly illustrate the technical scheme in the embodiment or prior art of the present application, the drawings needed to be used in the embodiment or prior art description will be briefly introduced as follows.

[0013] Fig. 1 It is the whole structure schematic diagram of a cell -free protein synthesis reactor of the utility model first embodiment.

[0014] Fig. 2 It is the front view of a cell -free protein synthesis reactor of the utility model first embodiment.

[0015] Fig. 3 is the overall structure diagram of the driving member of the first embodiment of the utility model.

[0016] In the figure: 100 - outer sleeve, 101 - protein synthesis tube, 102 - mounting ring, 103 - abutment plate, 104 - driving plate, 105 - mounting seat, 106 - threaded rod, 107 - rotating ring, 108 - driving gear, 109 - rubber pad, 110 - sliding block. DETAILED DESCRIPTION

[0017] The embodiments of the utility model are described in detail below, the examples of the embodiments are shown in the drawings, the embodiments described below by referring to the drawings are exemplary, and are intended to explain the utility model, and can not be understood as the limitation of the utility model.

[0018] The first embodiment of the application is:

[0019] Please refer to Figs. 1 to 3 , Fig. 1 is the overall structure diagram of a cell-free protein synthesis reactor of the first embodiment of the utility model, Fig. 2 is the front view of a cell-free protein synthesis reactor of the first embodiment of the utility model, Fig. 3 is the overall structure diagram of the driving member of the first embodiment of the utility model.

[0020] The utility model provides a kind of cell-free protein synthesis reactor, including outer sleeve 100, protein synthesis tube 101 and ejecting assembly, the ejecting assembly includes mounting ring 102, abutment plate 103, driving member and driving plate 104, the driving member includes mounting seat 105, threaded rod 106, transmission element and sliding block 110, the transmission element includes rotating ring 107, driving gear 108 and rubber pad 109, by this scheme, it solves that after reaction is completed, staff needs to spend larger force to be able to take out protein synthesis tube from outer sleeve, easy to cause the rupture of protein synthesis tube, the problem of lower safety.

[0021] For this specific embodiment, the protein synthesis tube 101 is mounted on the inner side of the outer sleeve 100, the protein synthesis tube 101 is the protein synthesis tube recorded in the existing patent technology CN219930090U cell-free protein synthesis reactor, the inner side of the outer sleeve 100 can contain protein synthesis reagent, the cooperation of the protein synthesis tube 101 and the outer sleeve 100 promotes reagent exchange, better promotes protein synthesis, and the protein synthesis tube and the outer sleeve are separated more stably and easily by the scheme, thereby improving the safety during use.

[0022] The installation ring 102 is fixedly connected with the protein synthesis pipe 101 and located on the side of the protein synthesis pipe 101 away from the outer sleeve 100, the abutment plate 103 is fixedly connected with the installation ring 102 and located on the side of the installation ring 102 away from the protein synthesis pipe 101, the driving plate 104 is connected with the outer sleeve 100 through the driving member, the driving member drives the driving plate 104 to move, the driving plate 104 abuts against the abutment plate 103, the installation ring 102 is installed on the outer side of the protein synthesis pipe 101 through bolts, the abutment plate 103 is installed on the side of the installation ring 102 away from the protein synthesis pipe 101 through bolts, so that the outer sleeve 100 can be located at the interlayer of the abutment plate 103 and the protein synthesis pipe 101, the driving plate 104 is installed on the outer side of the outer sleeve 100 through the driving member, the driving plate 104 is driven to move upward through the driving member, so that the driving plate 104 abuts against the abutment plate 103 and drives the abutment plate 103 to move upward, and then drives the protein synthesis pipe 101 to move upward, so that the protein synthesis pipe 101 moves out of the outer sleeve 100, so that the protein synthesis pipe and the outer sleeve pipe are more stably and easily separated, and the safety during use is improved.

[0023] Secondly, the mounting seat 105 is fixedly connected with the outer sleeve 100 and located on the side of the outer sleeve 100 close to the driving plate 104; the threaded rod 106 is rotatably connected with the mounting seat 105 and located on the side of the mounting seat 105 close to the driving plate 104 and connected with the driving plate 104, the transmission element is installed on the outer sleeve 100, the transmission element drives the threaded rod 106 to rotate, the mounting seat 105 is fixedly installed on the outer side of the outer sleeve 100 through bolts, the threaded rod 106 is installed on the mounting seat 105 through a bearing and extends into the inner side of the driving plate 104, the driving plate 104 has a threaded hole matched with the threaded rod 106, so that the threaded rod 106 can drive the driving plate 104 to move upward when rotating on the mounting seat 105, so as to eject the protein synthesis pipe 101, the transmission element is installed on the outer sleeve 100, the threaded rod 106 is driven to rotate through the transmission element, and then the driving plate 104 is driven to move.

[0024] Meanwhile, the rotating ring 107 is in sliding connection with the outer sleeve 100 and located at one side of the outer sleeve 100 close to the mounting base 105; the driving gear 108 is in fixed connection with the threaded rod 106 and located at one end of the threaded rod 106 close to the rotating ring 107 and connected with the rotating ring 107, the rotating ring 107 is rotatably installed on the outer sleeve 100, the outer sleeve 100 has a sliding groove matched with the rotating ring 107, so that the rotating ring 107 can stably slide on the outer sleeve 100, the driving gear 108 is fixedly installed at one side of the threaded rod 106 close to the rotating ring 107, the threaded rod 106 is driven to rotate through the driving gear 108, the driving gear 108 is in engagement with the rotating ring 107, the inner side of the rotating ring 107 has a gear groove matched with the driving gear 108, so that the rotating ring 107 can drive the driving gear 108 to rotate, thereby controlling the driving plate 104 to move up and down.

[0025] In addition, the rubber pad 109 is in fixed connection with the rotating ring 107 and located at one side of the rotating ring 107 away from the driving gear 108, the rubber pad 109 is installed on the outer side of the rotating ring 107 in a manner of adhesion, the friction between the rotating ring 107 and the hand is increased through the rubber pad 109, so that the staff can more stably rotate the rotating ring 107.

[0026] Finally, the sliding block 110 is in fixed connection with the driving plate 104 and located at one side of the driving plate 104 close to the outer sleeve 100 and connected with the outer sleeve 100, the sliding block 110 is fixedly installed at one side of the driving plate 104 close to the outer sleeve 100 through bolts, the outer sleeve 100 has a sliding groove matched with the sliding block 110, the movement of the driving plate 104 is limited through the sliding block 110, thereby making the threaded rod 106 more stably drive the driving plate 104 to move.

[0027] After the reaction is completed, the rotating ring 107 is rotated in the cell-free protein synthesis reactor of the embodiment, the rotating ring 107 drives the driving gear 108 to rotate, the driving gear 108 drives the threaded rod 106 to rotate on the mounting base 105, the driving plate 104 is driven to move upward through the threaded rod 106, thereby making the driving plate 104 abut against the abutting plate 103, thereby making the driving plate 104 top the protein synthesis tube 101 out of the outer sleeve 100 through the abutting plate 103, so that the protein synthesis tube and the outer sleeve are more stably and easily separated, thereby improving the safety during use.

[0028] The above disclosure only shows one or more preferred embodiments of the present application, and cannot limit the scope of the present application. Those skilled in the art can understand that the implementation of all or part of the above processes, and the equivalent changes made according to the claims of the present application, still belong to the scope covered by the present application.

Claims

1. A cell-free protein synthesis reactor, comprising an outer sleeve and a protein synthesis tube, the protein synthesis tube being installed on the inner side of the outer sleeve, characterized in that, It further comprises a pop-up assembly; The pop-up assembly comprises a mounting ring, an abutment plate, a driving member and a driving plate, the mounting ring is fixedly connected with the protein synthesis tube and located on the side of the protein synthesis tube away from the outer sleeve, the abutment plate is fixedly connected with the mounting ring and located on the side of the mounting ring away from the protein synthesis tube, the driving plate is connected with the outer sleeve through the driving member, the driving member drives the driving plate to move, and the driving plate abuts against the abutment plate.

2. The cell-free protein synthesis reactor of claim 1, characterized in that, The driving member comprises a mounting seat, a threaded rod and a transmission element, the mounting seat is fixedly connected with the outer sleeve and located on the side of the outer sleeve close to the driving plate; the threaded rod is rotatably connected with the mounting seat and located on the side of the mounting seat close to the driving plate, and connected with the driving plate, the transmission element is installed on the outer sleeve, and the transmission element drives the threaded rod to rotate.

3. The cell-free protein synthesis reactor of claim 2, characterized in that, The transmission element comprises a rotating ring and a driving gear, the rotating ring is slidably connected with the outer sleeve and located on the side of the outer sleeve close to the mounting seat; the driving gear is fixedly connected with the threaded rod and located on the end of the threaded rod close to the rotating ring, and connected with the rotating ring.

4. The cell-free protein synthesis reactor of claim 3, characterized in that, The transmission element further comprises a rubber pad, the rubber pad is fixedly connected with the rotating ring and located on the side of the rotating ring away from the driving gear.

5. The cell-free protein synthesis reactor of claim 1, characterized in that, The driving member further comprises a sliding block, the sliding block is fixedly connected with the driving plate and located on the side of the driving plate close to the outer sleeve, and connected with the outer sleeve.

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