Oscillating device for DNA methylation test

Abstract

The utility model relates to an oscillation device for DNA methylation test, which comprises a working box, the top of the working box is hinged with a box cover, the inside of the working box is rotatably connected with a plurality of rollers, the tops of the rollers are movably contacted with a storage box, the back of the storage box is provided with a driving assembly for driving the storage box to oscillate, and the driving assembly is connected with the working box. The top of the storage box is movably connected with a protective cover, two limiting rods are fixed to the bottom of the protective cover, separation assemblies are arranged on the left side and the right side of the storage box, and a positioning mechanism is arranged on the inner bottom wall of the storage box. According to the oscillation device for the DNA methylation test, a sample tube is placed on the positioning mechanism, through operation of the positioning mechanism, the sample tube can be fixed, then the protective cover is covered, the box cover is closed, and the driving assembly is started; the driving assembly can drive the storage box to move left and right, so that the purpose of stably oscillating the plurality of sample tubes is achieved.

Description

TECHNICAL FIELD

[0001] The utility model relates to DNA methylation detection technical field, concretely is a kind of DNA methylation test with shock device. BACKGROUND

[0002] DNA methylation is an important mechanism of gene expression regulation, and DNA methylation detection refers to determining the degree of DNA methylation in tumor cells using various methods. In DNA methylation test experiments, sample tube mixing is a common experimental operation.

[0003] Currently, the commonly used test reagent requires the operator to hold the sample tube and perform shaking mixing. Each time, only a single test tube can be shaken, and when shaking, if the operator does not hold the sample tube firmly, the sample tube may fall, causing sample contamination and damage. Therefore, a DNA methylation test shock device is proposed to solve the above problems. UTILITY MODEL CONTENT

[0004] In view of the deficiencies of the prior art, the utility model provides a DNA methylation test shock device, which has the advantages of stable shaking and solves the problems of sample contamination and damage.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: a DNA methylation test shock device, comprising a working box, a box cover is hingedly connected to the top of the working box, a plurality of rollers are rotatably connected inside the working box, a storage box is movably connected to the top of the roller, a driving assembly for driving the storage box to shake is arranged on the back of the storage box, a protective cover is movably connected to the top of the storage box, two limiting rods are fixed to the bottom of the protective cover, a separation assembly is arranged on the left and right sides of the storage box, and a positioning mechanism is arranged on the inner bottom wall of the storage box.

[0006] The positioning mechanism comprises a storage seat fixed to the inner bottom wall of the storage box, a second motor is fixed to the right side wall of the inner cavity of the storage seat, a threaded rod is fixed to the output shaft of the second motor, a support seat is sleeved on the outer surface of the threaded rod and fixed to the inner bottom wall of the storage seat, a plurality of moving rods are fixed to the outer surface of the threaded rod and penetrate through the top of the storage seat, and two clamping plates are fixed between each group of moving rods.

[0007] Further, the separation assembly comprises a button movably connected to the left and right sides of the storage box, two springs are fixed to the opposite side of each button, and a push rod is fixed to the opposite side of each button.

[0008] Further, the driving assembly comprises a protection bin fixed to the back of the working box, a first motor is fixed to the rear sidewall of the inner cavity of the protection bin, an output shaft of the first motor is fixed with a rotating rod penetrating into the inner cavity of the working box, a driving gear is fixed to the front side of the rotating rod, and the rear sidewall of the inner cavity of the working box is provided with a moving structure meshing with the driving gear.

[0009] Further, the moving structure comprises two positioning rails fixed to the rear sidewall of the inner cavity of the working box, and the opposite sides of the two positioning rails are both slidably connected with a rack meshing with the driving gear, the left and right sides of the rack are both fixed with a connecting rod, and the front side of the connecting rod is fixed with two link rods fixed to the back of the storage box.

[0010] Further, the top of the storage box is provided with a limiting groove matched with the limiting rod, and the bottom of the protection cover is fixed with a sealing gasket.

[0011] Further, the threaded rod is fixed with seven groups of threaded rods with opposite directions at two ends, and the moving rod is provided with threaded through holes matched with the corresponding threaded rods.

[0012] Further, the top of the storage seat is provided with a storage groove, and the bottom of the storage groove is fixed with a shock pad.

[0013] Compared with the prior art, the technical scheme has the following beneficial effects:

[0014] The DNA methylation test shock device is used for placing the sample tube on the positioning mechanism, and the sample tube can be fixed through the operation of the positioning mechanism. The protection cover is covered, the box cover is closed, the driving assembly is started, the driving assembly can drive the storage box to move left and right through the operation of the driving assembly, and the purpose of stable shock of the plurality of sample tubes is achieved. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a structural schematic view of the utility model;

[0016] Figure 2 It is the utility model Figure 1 It is an enlarged view of A in the utility model;

[0017] Figure 3 It is a structural schematic view of the utility model working box;

[0018] Figure 4 It is a side view of the utility model working box;

[0019] Figure 5 It is a structural schematic view of the positioning mechanism of the utility model;

[0020] Figure 6The utility model discloses a three-dimensional appearance view of the positioning mechanism.

[0021] In the drawing: 1 work box, 2 box cover, 3 roller, 4 storage box, 5 protective cover, 6 limiting rod, 7 button, 8 spring, 9 push rod, 10 positioning mechanism, 1001 storage seat, 1002 second motor, 1003 threaded rod, 1004 support seat, 1005 moving rod, 1006 clamping plate, 1007 shock pad, 11 protective bin, 12 first motor, 13 rotating rod, 14 drive gear, 15 positioning rail, 16 rack, 17 connecting rod, 18 link rod. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0023] Please refer to Figures 1-4 In the embodiment, the DNA methylation test shock device includes a work box 1, the top of the work box 1 is hinged with a box cover 2, a plurality of rollers 3 are rotationally connected in the work box 1, the top of the roller 3 movably contacts a storage box 4, a limiting groove matched with the limiting rod 6 is formed in the top of the storage box 4, a driving assembly for driving the storage box 4 to shake is arranged on the back of the storage box 4, the bottom of the protective cover 5 movably connected with the top of the storage box 4 is fixed with a sealing gasket, the sealing gasket can seal the top of the sample tube, and the sample in the sample tube can be prevented from spilling out during shaking, the bottom of the protective cover 5 is fixed with two limiting rods 6, and a separation assembly is arranged on the left and right sides of the storage box 4, the separation assembly includes a button 7 movably connected with the left and right sides of the storage box 4, the opposite side of the two buttons 7 is fixed with two springs 8, the opposite side of the two buttons 7 is fixed with a push rod 9, when the protective cover 5 needs to be opened, the button 7 is pressed to drive the push rod 9 to move and push the limiting rod 6, so that the limiting rod 6 is separated from the limiting groove, and the protective cover 5 is separated from the storage box 4, and the inner bottom wall of the storage box 4 is provided with a positioning mechanism 10.

[0024] The driving assembly in the embodiment comprises a protective bin 11 fixed to the back of the working box 1, the rear side wall of the inner cavity of the protective bin 11 is fixed with a first motor 12, the output shaft of the first motor 12 is fixed with a rotating rod 13 penetrating into the inside of the working box 1, the front side of the rotating rod 13 is fixed with a driving gear 14, the driving gear 14 is a half-tooth gear, when the driving gear 14 rotates, the driving gear 14 can always mesh with the rack 16 on one side, so that the moving structure can move back and forth, and the rear side wall of the inner cavity of the working box 1 is provided with a moving structure meshing with the driving gear 14.

[0025] The moving structure comprises two positioning rails 15 fixed to the rear side wall of the inner cavity of the working box 1, the opposite sides of the two positioning rails 15 are both slidably connected with the rack 16 meshing with the driving gear 14, the left and right sides of the rack 16 are both fixed with a connecting rod 17, and the front side of the connecting rod 17 is fixed with two connecting rods 18 fixed to the back of the storage box 4.

[0026] It should be noted that the rotating rod 13 can drive the driving gear 14 to rotate through the operation of the first motor 12, the driving gear 14 meshes with the rack 16, and when the driving gear 14 rotates, the rack 16 can be driven to move left and right, so that the driving assembly can drive the storage box 4 to move left and right as a whole, so that the oscillation device can normally oscillate the sample.

[0027] Please refer to Figures 5-6 , in order to stably oscillate multiple samples, the positioning mechanism 10 in the embodiment comprises a storage seat 1001 fixed to the inner bottom wall of the storage box 4, the top of the storage seat 1001 is provided with a storage groove, the bottom of the storage groove is fixed with a damping pad 1007, the damping pad 1007 can increase the friction between the sample tube and the storage groove, so that the sample tube will not collide with the inner wall of the storage groove when oscillating the sample, thereby improving the stability of the oscillation device during use, the right side wall of the inner cavity of the storage seat 1001 is fixed with a second motor 1002, the output shaft of the second motor 1002 is fixed with a threaded rod 1003, the threaded rod 1003 is fixed with seven groups of threaded rods with opposite directions at both ends, through the setting of the reverse threaded rods, the corresponding moving rods 1005 can move relatively, so that the moving rods 1005 can drive the clamping plates 1006 to clamp and fix the sample tubes, the outer surface of the threaded rod 1003 is sleeved with a support seat 1004 fixed to the inner bottom wall of the storage seat 1001, the outer surface of the threaded rod 1003 is threadedly connected with multiple groups of moving rods 1005 penetrating through the top of the storage seat 1001, the moving rods 1005 are provided with threaded holes corresponding to the threaded rods, and each group of moving rods 1005 is fixed with two clamping plates 1006.

[0028] It should be noted that the threaded rod 1003 is rotated by the operation of the second motor 1002, the moving rod 1005 is moved by the rotation of the threaded rod 1003, the two clamping plates 1006 can clamp and fix the sample tube by the movement of the moving rod 1005, and the object seat 1001 is provided with a plurality of object grooves for placing sample tubes, so that the positioning mechanism 10 can fix a plurality of sample tubes at a time.

[0029] The electric elements in the text are all electrically connected with the controller and the power supply. The control mode of the utility model is controlled by the controller. The control circuit of the controller can be realized by simple programming of the skilled person in the art. The power supply also belongs to the common knowledge in the art. And the utility model is mainly used for protecting mechanical devices, so the control mode and circuit connection of the utility model will not be explained in detail.

[0030] The working principle of the above embodiment is as follows:

[0031] In use, the sample tube is placed in the object groove on the object seat 1001, the second motor 1002 is started to rotate the threaded rod 1003, the moving rod 1005 is moved by the rotation of the threaded rod 1003, so that the moving rod 1005 can drive the clamping plate 1006 to move, so that the clamping plate 1006 can clamp the sample tube, after clamping the sample tube, the limiting rod 6 on the protective cover 5 is aligned with the limiting groove on the top of the object box 4 and put in, the protective cover 5 can be connected with the object box 4 by the connection of the limiting rod 6 and the limiting groove, then the box cover 2 is closed, the first motor 12 is started to drive the rotating rod 13 to rotate, so that the rotating rod 13 can drive the driving gear 14 to rotate, the driving gear 14 can drive the rack 16 to move by the meshing of the driving gear 14 and the rack 16, so that the rack 16 can drive the connecting rod 17 to move left and right, the object box 4 can be moved left and right by the action of the connecting rod 17, so as to realize the purpose of stable oscillation of a plurality of sample tubes.

[0032] It is to be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. An element proceeded by "comprises... a" does not, without more constraints, exclude the presence of additional identical elements in the process, method, article, or apparatus that comprises the element.

[0033] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the application.

Claims

1. A shock device for DNA methylation test, comprising a working box (1), characterized in that: The top of the working box (1) is hinged with a box cover (2), a plurality of rollers (3) are rotatably connected in the working box (1), the top of the roller (3) movably contacts with a storage box (4), the back of the storage box (4) is provided with a driving assembly for driving the storage box (4) to oscillate, the top of the storage box (4) movably connects with a protective cover (5), the bottom of the protective cover (5) is fixed with two limiting rods (6), the left and right sides of the storage box (4) are provided with a separation assembly, and the inner bottom wall of the storage box (4) is provided with a positioning mechanism (10). The positioning mechanism (10) comprises a storage seat (1001) fixed to the inner bottom wall of the storage box (4), a second motor (1002) fixed to the right side wall of the inner cavity of the storage seat (1001), a threaded rod (1003) fixed to the output shaft of the second motor (1002), a support seat (1004) sleeved on the outer surface of the threaded rod (1003) and fixed to the inner bottom wall of the storage seat (1001), and a plurality of moving rods (1005) penetrating through the top of the storage seat (1001) and being threadedly connected to the outer surface of the threaded rod (1003). Each group of moving rods (1005) is fixed with two clamping plates (1006).

2. The shaking device for DNA methylation test according to claim 1, characterized in that: The separation assembly comprises a button (7) movably connected to the left and right sides of the storage box (4), two springs (8) fixed to the opposite side of each of the two buttons (7), and a push rod (9) fixed to the opposite side of each of the two buttons (7).

3. The shaking device for DNA methylation test according to claim 1, characterized in that: The driving assembly comprises a protective bin (11) fixed to the back of the working box (1), a first motor (12) fixed to the rear side wall of the inner cavity of the protective bin (11), a rotating rod (13) penetrating into the inner cavity of the working box (1) and fixed to the output shaft of the first motor (12), a driving gear (14) fixed to the front side of the rotating rod (13), and a moving structure arranged on the rear side wall of the inner cavity of the working box (1) and meshing with the driving gear (14).

4. The shaking device for DNA methylation test according to claim 3, characterized in that: The moving structure comprises two positioning rails (15) fixed to the rear side wall of the inner cavity of the working box (1), a rack (16) movably connected to the opposite side of each of the two positioning rails (15) and meshing with the driving gear (14), a connecting rod (17) fixed to the left and right sides of the rack (16), and two link rods (18) fixed to the front of the connecting rod (17) and fixed to the back of the storage box (4).

5. The shaking device for DNA methylation test according to claim 1, characterized in that: The top of the storage box (4) is provided with a limiting groove matched with the limiting rod (6), and the bottom of the protective cover (5) is fixed with a sealing gasket.

6. The shaking device for DNA methylation test according to claim 1, characterized in that: Seven groups of thread strips with opposite directions at both ends are fixed on the threaded rod (1003), and a threaded hole is formed in the moving rod (1005) and matched with the corresponding thread strip.

7. The shaking device for DNA methylation test according to claim 1, characterized in that: A storage groove is formed in the top of the storage seat (1001), and a damping pad (1007) is fixed to the bottom of the storage groove.

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