Kit for ultrasensitive detection of PLA2R-IgG antibody
By introducing a foldable handle, sliding rail structure, and positioning slot into the PLA2R-IgG TRFIA detection kit, the problems of unreasonable internal space design and insufficient portability of the kit have been solved, thereby improving the stability and portability of the components.
Patent Information
- Application Number
- CN202520485834.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-19
- Publication Date
- 2026-03-20
- Estimated Expiration
- 2035-03-19
AI Technical Summary
Existing PLA2R-IgG TRFIA detection kits have an unreasonable internal space design, which can lead to component collision damage, and they are not portable enough, affecting the accuracy of test results and the stability of transportation.
A reagent kit was designed, which includes a foldable handle, a sliding rail structure, and positioning slots. The horizontal box and the bottom box are connected by the sliding rail, and the positioning slots are set to fix the reagent bottles and reaction plates. ABS plastic and polyurethane foam insulation layer are used to ensure the stability and portability of the components.
This improves the stability and portability of the reagent kit during transportation, avoids damage from component collisions, and ensures the accuracy of test results and safety during transportation.
Smart Images
Figure CN224014239U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the medical detection technical field relates to a kind of antibody detection kit, specifically relates to a kind of kit for the super-sensitive detection of PLA2R-IgG antibody. BACKGROUND
[0002] The continuous improvement of modern medical detection and diagnosis technology makes many diseases more accurate diagnosis, among them, immune analysis antibody detection technology plays an important role in disease diagnosis. Idiopathic membranous nephropathy (IMN) is one of the most common pathological types of adult nephrotic syndrome, and is clinically manifested as massive proteinuria and nephrotic syndrome. PLA2R-IgG antibody detection is a specific indicator for diagnosing idiopathic membranous nephropathy (IMN), and the detection result of PLA2R-IgG antibody can determine whether the patient has membranous nephropathy. Current research believes that the anti-PLA2R antibody (anti-phospholipase A2 receptor antibody) of membranous nephropathy patients is closely related to the occurrence and development of the disease, and the anti-PLA2R antibody is expressed on the surface of human glomerular podocytes. In the membranous nephropathy patients positive for anti-phospholipase A2 receptor antibody, antigen / antibody complexes are deposited on the glomerular basement membrane, activating the complement system, ultimately causing damage to podocytes and glomerular filtration barrier, protein entering the original urine, and then proteinuria occurs. Clinical application results show that anti-PLA2R antibody has good sensitivity and specificity in the diagnosis of primary membranous nephropathy, and is suitable for serological auxiliary diagnosis of primary membranous nephropathy. The positive rate of serum anti-PLA2R antibody in patients with primary membranous nephropathy is significantly higher than that in patients with secondary membranous nephropathy and other glomerulonephritis, and the level of anti-PLA2R antibody is positively correlated with the amount of urine protein in patients with primary membranous nephropathy. Many hospitals have developed anti-phospholipase A2 receptor antibody IgG (PLA2R-IgG) detection, an antibody detection project, for the diagnosis and differential diagnosis of primary membranous nephropathy. When massive proteinuria occurs in clinic, anti-phospholipase A2 receptor antibody IgG (PLA2R-IgG) will be detected, and if the antibody is positive, membranous nephropathy may be considered to be large, with an accuracy rate of 90%. By detecting the level of PLA2R-IgG antibody in the serum of patients, the type of nephropathy can be quickly and accurately determined, so as to guide doctors to develop effective treatment plans and improve the quality of life of patients.
[0003] There are many methods for detecting PLA2R-IgG antibody in serum, including enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), indirect immunofluorescence (IFA), time-resolved fluorescence immunoassay (TRFIA), etc. Compared with other antibody detection methods, time-resolved fluorescence immunoassay (TRFIA) has the advantages of high sensitivity and wide detection range.
[0004] The current research and application show that the time-resolved fluorescence immunoassay (TRFIA) with high sensitivity and wide detection range is used to quantitatively detect the PLA2R-IgG4 antibody level and the IgG4 antibody level specific to different domains of PLA2R of the IMN (idiopathic membranous nephropathy) patient, and the method is more helpful for accurate diagnosis of the disease of the patient and evaluation of the treatment effect and the prognosis. In the TRFIA (time-resolved fluorescence immunoassay) detection of the PLA2R-IgG, the detection kit is a carrier of the detection reagent and the reagent bottle, the reaction plate, the calibrant and other key components, and the detection kit is a very important link.
[0005] The existing detection kit for the TRFIA (time-resolved fluorescence immunoassay) detection of the PLA2R-IgG has many problems to be solved in actual application. First, the internal space of the kit is not reasonably designed. The traditional kit is mostly a single cavity structure, and the reagent bottle, the reaction plate, the standard component and other components are mixed and placed, and effective separation is lacked. In the transportation process, the components collide with each other, and the reagent bottle is easily damaged and the reaction plate is easily contaminated, thereby affecting the accuracy of the detection result. Second, the kit is not portable enough. Most of the kits are not provided with a special carrying structure, and the medical staff or the detection personnel can only hold the side of the box body when carrying, which is not convenient and the kit is easily dropped due to hand slipping. In view of the above defects and deficiencies in the prior art, the technical field urgently needs a kit for the TRFIA (time-resolved fluorescence immunoassay) super-sensitive detection of the PLA2R-IgG, which is reasonably designed, structurally optimized and functionally perfect, to meet the needs of the kit for portability and stability in actual detection work. Practical new type content
[0006] The technical problem to be solved by the utility model is that the kit is not reasonably designed in the internal space and the kit is not portable enough, and the kit for the super-sensitive detection of the PLA2R-IgG antibody is reasonably designed, structurally optimized and functionally perfect, to meet the needs of the kit for portability and stability in actual detection work.
[0007] The utility model adopts the following technical scheme to solve the technical problem:
[0008] The utility model provides a kit for PLA2R-IgG antibody ultra-sensitive detection, including kit main part namely box (1), the box (1) is cuboid shape, the front side of box (1) is equipped with box cover door (2), and one end of box cover door (2) is hinged (is connected through hinge or hinge) with one end of box (1), and the box (1) is equipped with foldable handle (3), and the middle position of the outer surface of the left and right sides of box (1) is fixedly installed with rotary connecting piece (4), and the both ends of foldable handle (3) are rotatably connected with the rotary connecting piece (4) of the outer surface of the left and right sides of box (1) through rotary connecting piece (4), and the inner wall of the left and right sides of box (1) is fixedly installed with a slide rail (7) with recess in upper position and bottom position, and the recess of slide rail (7) in upper position is connected with cross box (5), and cross box (5) is a certain thickness rectangular flat plate, and the both sides of cross box (5) are inserted into the recess of slide rail (7) and can slide in the recess of slide rail (7), and the upper surface of cross box (5) is provided with rectangular positioning recess (6) for placing polystyrene hole plate, and the recess of slide rail (7) in bottom position is connected with bottom box (8), and bottom box (8) is cuboid shape, and the both sides of bottom end are equipped with convex strip, and the both sides of convex strip of bottom box (8) are inserted into the recess of slide rail (7), and bottom box (8) can slide in the recess of slide rail (7), and the upper surface of bottom box (8) is provided with a plurality of circular positioning hole slots (9) for placing reagent bottle for placing reagent or standard sample, and is also provided with rectangular positioning hole slot (10) for placing ice bag, and the thickness of bottom box (8) is much larger than the thickness of cross box (5), and the depth of circular positioning hole slot (9) and rectangular positioning hole slot (10) on bottom box (8) is much larger than the depth of rectangular positioning recess (6) on cross box (5).
[0009] Preferably, the rotary connecting piece (4) includes a fixed end bearing and a movable end rotating shaft; the box (1) is provided with an installation hole in the middle position of the left and right sides; the fixed end bearing of the rotary connecting piece (4) is embedded in the installation hole (with interference fit); the left and right rotary connecting pieces (4) are respectively fixedly installed on the outer surfaces of the left and right sides of the box (1) by embedding the fixed end bearings thereof into the installation holes (with interference fit); the both ends of the foldable handle (3) are fixedly connected with the movable end rotating shafts of the rotary connecting pieces (4) on the outer surfaces of the left and right sides of the box (1), so that the foldable handle (3) can be rotated.
[0010] More preferably, the both ends of the foldable handle (3) are provided with connecting holes; the both ends of the foldable handle (3) are sleeved on the rotating shafts through the connecting holes; the outer ends of the rotating shafts are provided with threads; fixed bolts are installed on the outer ends of the rotating shafts; the foldable handle (3) can be fixed with the rotary connecting piece (4) and rotatably connected with the box (1) by tightening the fixed bolts.
[0011] Preferably, the box cover door (2) is connected with the box (1) through a stainless steel hinge, which is corrosion-resistant and has a long service life.
[0012] More preferably, the box cover door (2) is provided with a magnet in the box body (1), and the opening and closing between the box cover door (2) and the box body (1) is achieved by magnetic attraction and adsorption.
[0013] Preferably, the foldable handle (3) is made of medical grade ABS plastic, and the surface is smooth; the foldable handle (3) is connected to the side of the box body (1) through a rotating connecting piece containing a bearing, a rotating shaft and a fixed bolt, and can be folded by 180 degrees; when carrying, it is perpendicular; when not in use, it is attached to the side of the box body (1). The foldable handle (3) is in the shape of a circular arch, the middle arch of the circular arch is a hand holding part, and the two ends of the circular arch are connected to the two sides of the box body (1) through rotating connecting pieces containing bearings, rotating shafts and fixed bolts; the foldable handle (3) can be folded by 180 degrees; when carrying, the foldable handle (3) is lifted vertically to the box body (1) to stand upright, and the middle arch of the handle, that is, the hand holding part, is above the box body (1), which is convenient for holding and carrying; when not in use, the foldable handle (3) is folded and placed close to the side of the box body (1), and the middle arch of the handle, that is, the hand holding part, is placed at the back side of the box body (1), which does not affect the opening of the door of the box body (1) to take and place articles.
[0014] Preferably, the inner wall of the box body (1) is provided with a heat preservation and insulation layer. The inner wall of the box body (1) is pasted with a polyurethane foam heat preservation and insulation layer, and the inside of the box body (1) is pasted with polyurethane foam, which has excellent heat preservation and insulation effect, light weight and waterproof, and forms a continuous heat preservation structure.
[0015] Preferably, the box body (1) is integrally formed by high-strength engineering plastic, and has a cuboid structure, the size conforms to ergonomics, and the wall thickness is calculated by mechanics, so that light weight and pressure resistance are considered.
[0016] Preferably, the upper surface of the horizontal box (5) is provided with two large rectangular positioning grooves (6) for placing polystyrene well plates for detection; the upper surface of the bottom box (8) is provided with a plurality of circular positioning hole grooves (9) with different inner diameters for placing reagent bottles for reagents or standard samples, and is also provided with a rectangular positioning hole groove (10) for placing an ice bag.
[0017] Preferably, the upper surface of the bottom box (8) is also provided with a ground glass treated rectangular label sticking area, and the label is used for marking the name of the reagent box, the detection item, the valid period and the like, so as to facilitate identification and management.
[0018] More preferably, the inner wall of the circular positioning hole groove (9) for placing the reagent bottle for reagents or standard samples is pasted with a 2-3mm thick silica gel elastic buffer layer, which functions to fix the reagent bottle and absorb vibration.
[0019] The utility model discloses the beneficial effects of:
[0020] The utility model provides a design is reasonable, structural optimization, function perfect for PLA2R-IgG antibody hypersensitive detection's kit, the kit is set up through installation slide rail (7), cross box (5), bottom box (8), positioning hole groove, foldable handle (3) etc.
[0021] The utility model discloses a kit for PLA2R-IgG antibody hypersensitive detection has the following beneficial effects:
[0022] (I) the kit side is equipped with foldable handle (3), adopts ABS plastic, and the weight is light and high in strength.
[0023] (II) through the design humanization foldable handle (3), has improved the carrying comfort.
[0024] (III) foldable handle (3) is connected with box (1) through the rotary connecting piece containing the bearing, and the angle can be flexibly adjusted: when carrying, vertically lifts, conforms to human engineering, and the stress is evenly dispersed to the palm, reduces the hand compression feeling;When not using, foldable and adhere to the side of box (1), avoid occupying additional space, facilitate stacking transportation or storage.
[0025] (Four) the size of box (1) is optimized, satisfies the internal component containing demand, also control overall volume. With foldable handle (3), when the kit is transported in the vehicle, is shipped by air or is manually carried, can be easily put into medical kit, backpack and other containers.
[0026] (V) the cross box (5) and bottom box (8) in the kit are connected with box (1) through slide rail (7), and the extraction is convenient.
[0027] (Six) the upper surface of cross box (5) and bottom box (8) is equipped with a plurality of positioning hole grooves, such as circular positioning hole groove (9) adapts reagent bottle, rectangular positioning hole groove (6) adapts reaction plate (polystyrene hole plate), and rectangular positioning hole groove (10) adapts ice bag;The inner wall of positioning hole groove is additionally provided with elastic buffer layer. During transportation, after reagent bottle is inserted into positioning hole groove, is tightly wrapped, and elastic buffer layer absorbs vibration energy, ensures that the component displacement amount is controlled in a very small range (such as within ±1mm). Even if experiencing bumpy road conditions or carrying collision, internal components can remain stable, avoid the occurrence of events such as mutual extrusion, dumping or damage. BRIEF DESCRIPTION OF DRAWINGS
[0028] Figure 1 It is the external structure schematic diagram of the utility model;
[0029] Figure 2It is the internal structure schematic diagram of the utility model;
[0030] Figure 3 It is the internal box structure schematic diagram of the utility model.
[0031] In the drawing, 1, box body; 2, box cover door; 3, foldable handle; 4, rotary connecting piece; 5, horizontal box; 6, rectangular positioning groove; 7, slide rail; 8, bottom box; 9, circular positioning hole groove; 10, rectangular positioning hole groove. DETAILED DESCRIPTION
[0032] In order to make the technical problems, technical schemes and beneficial effects to be solved by the utility model more clear, the following will be combined with the drawings and examples, and the utility model will be further more detailed, and the technical scheme in the utility model example will be clearly and completely described, obviously, the described example is a part of the utility model example, not all examples. The components of the utility model example described and shown in the following examples and drawings can be arranged and designed in various different configurations.
[0033] The detailed description of the utility model example and the components in the following drawings is not intended to limit the protection scope of the claimed utility model, but only represents the selected example of the utility model. Based on the example in the utility model, all other examples obtained by the ordinary skilled person in the art without creative labor belong to the protection scope of the utility model.
[0034] It should be noted that: similar signs and letters represent similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second" and the like are only used for distinguishing description, and cannot be understood as indicating or implying relative importance.
[0035] It should be noted that: similar signs and letters represent similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second" and the like are only used for distinguishing description, and cannot be understood as indicating or implying relative importance.
[0036] Example 1
[0037] As Figure 1 , Figure 2 , Figure 3As shown, the utility model relates to a kit for super-sensitive detection of PLA2R-IgG antibody, which comprises a kit main body, i.e. a box body 1; the box body 1 is in the shape of a cuboid, a box cover door 2 is arranged on the front side of the box body 1, one end of the box cover door 2 is hinged (connected through a hinge) to one end of the box body 1; a foldable handle 3 is arranged on the box body 1; a rotating connecting piece 4 is fixedly installed at the middle position of the left and right sides of the box body 1; the two ends of the foldable handle 3 are rotatably connected to the box body 1 through the rotating connecting pieces 4 on the outer surfaces of the left and right sides of the box body 1; a groove-equipped slide rail 7 is fixedly installed at the upper position and the bottom position on the inner walls of the left and right sides of the box body 1; a transverse box 5 is connected in the groove of the slide rail 7 at the upper position; the transverse box 5 is a flat plate in the shape of a rectangle with a certain thickness; the two side ends of the transverse box 5 are inserted into the groove of the slide rail 7 and can slide in the groove of the slide rail 7; a rectangular positioning groove 6 is formed in the upper surface of the transverse box 5 and is used for mounting and placing a polystyrene well plate; a bottom box 8 is connected in the groove of the slide rail 7 at the bottom position; the bottom box 8 is in the shape of a cuboid; protrusions are arranged on the two side ends of the bottom box 8; the protrusions on the bottom box 8 are inserted into the groove of the slide rail 7, so that the bottom box 8 can slide in the groove of the slide rail 7; a plurality of circular positioning holes 9 and a rectangular positioning hole 10 are formed in the upper surface of the bottom box 8; the circular positioning holes 9 are used for placing reagent bottles for reagents or standard products; and the rectangular positioning hole 10 is used for placing an ice bag.
[0038] The rotating connecting piece 4 comprises a fixed end bearing and a movable end rotating shaft; installation holes are formed at the middle positions of the left and right sides of the box body 1; the fixed end bearing of the rotating connecting piece 4 is embedded in the installation hole (with interference fit); and the left and right rotating connecting pieces 4 are fixedly installed on the outer surfaces of the left and right sides of the box body 1 by embedding the fixed end bearings thereof into the installation holes (with interference fit); the two ends of the foldable handle 3 are fixedly connected to the movable end rotating shafts of the rotating connecting pieces 4 on the outer surfaces of the left and right sides of the box body 1, so that the foldable handle 3 can be rotated; connection holes are arranged at the two ends of the foldable handle 3; the two ends of the foldable handle 3 are sleeved on the rotating shafts through the connection holes; the outer ends of the rotating shafts are provided with threads; fixed bolts are arranged on the outer ends of the rotating shafts; and the foldable handle 3 and the rotating connecting piece 4 can be fixed by tightening the fixed bolts, and the foldable handle 3 and the box body 1 can be rotatably connected.
[0039] The box body 1 is integrally formed by using high-strength engineering plastic and is in the shape of a cuboid; the optimal size is 280*160*200mm; the box body 1 meets the ergonomics; the wall thickness is 3mm after mechanical calculation; and the box body 1 has light weight and pressure resistance.
[0040] The inner wall of the box body 1 is provided with a heat insulation layer; a polyurethane foam layer is attached to the inner wall of the box body 1; the inside of the box body 1 is attached with a polyurethane foam; the heat insulation effect is excellent; the texture is light and waterproof; and a continuous heat insulation structure is formed.
[0041] The box cover door 2 is connected with the box body 1 through a stainless steel hinge, the hinge is corrosion resistant and has a long service life. Magnets are arranged in the box cover door 2 and the box body 1, and the opening and closing of the box cover door 2 and the box body 1 is achieved through magnetic attraction.
[0042] Foldable handles 3 are fixed on the two sides of the box body 1; the foldable handles 3 are made of medical-grade ABS plastic and have a smooth surface; the foldable handles 3 are in the shape of a circular arc arch, the middle arch top of the circular arc arch is a hand holding part, and the two ends of the circular arc arch are connected with the two sides of the box body 1 through rotating connecting pieces containing bearings, rotating shafts and fixing bolts; the foldable handles 3 can be rotated and folded by 180 degrees, when carrying and using, the foldable handles 3 are lifted to be perpendicular to the box body 1 to be in a vertical state, and the middle arch top of the handle, i.e. the hand holding part, is located above the box body 1, so that the handle is convenient to hold and carry; when not carrying and using, the foldable handles 3 are folded and placed close to the sides of the box body 1, and the middle arch top of the handle, i.e. the hand holding part, is placed at the back side of the box body 1, so that the box body 1 is not affected to open the door to take and place articles.
[0043] The upper surface of the horizontal box 5 is provided with two large rectangular positioning grooves 6 for placing polystyrene hole plates for detection; the upper surface of the bottom box 8 is provided with a plurality of circular positioning hole grooves 9 of different inner diameters for placing reagent bottles containing reagents or standard samples, and is also provided with rectangular positioning hole grooves 10 for placing ice bags; the upper surface of the bottom box 8 is also provided with a rectangular label sticking area with frosted treatment, the label is used for marking the name of the reagent box, the detection item, the validity period and the like, so as to be convenient for identification and management. A 3mm thick silica gel elastic buffer layer is pasted on the inner wall of the circular positioning hole groove 9 for placing the reagent bottles containing reagents or standard samples, which functions to fix the reagent bottles and absorb vibration. The thickness of the bottom box 8 is much larger than that of the horizontal box 5. The depth of the circular positioning hole groove 9 and the rectangular positioning hole groove 10 on the bottom box 8 is much larger than that of the rectangular positioning groove 6 on the horizontal box 5.
[0044] The box body 1 is integrally assembled: the box body 1 is made of ABS material and is injection molded, with a wall thickness of 3 mm, giving consideration to light weight and pressure resistance. The box cover door 2 is connected to the box body 1 through a stainless steel hinge, and the hinge is fixed to the side of the box body 1 through a screw, and a reserved hole is provided at the edge of the box cover door 2, the opening and closing smoothness is tested, and the rotation angle is ensured to be greater than 120 degrees. The foldable handle 3 is an arc-shaped structure and is made of medical-grade ABS plastic material, and can bear a weight of 5 kg without deformation; the rotating connecting piece 4 comprises a metal rolling bearing, a rotating shaft and a fixing bolt; the metal rolling bearing of the rotating connecting piece 4 is embedded into the mounting hole of the box body 1 (with interference fit), the inner ring of the bearing is sleeved on the rotating shaft, the two ends of the foldable handle 3 are connected to the rotating shaft of the rotating connecting piece 4, the outer end of the rotating shaft is provided with a thread, the fixing bolt is tightened for fixed connection, the rotating function is debugged, and it is ensured that the foldable handle 3 can be rotated and folded by 180 degrees and can bear a weight of 5 kg without deformation. Positioning hole grooves are provided according to the sizes of reagent bottles and reaction plates, and a 3 mm thick silica gel buffer layer (fixed by double-sided adhesive tape) is pasted on the inner wall of the hole to tightly wrap the components to prevent shock. C-shaped sliding rails 7 are arranged on the inner wall of the box body 1, the horizontal box 5 and the bottom box 8 are slidably matched with the sliding rails 7, and a 10 cm*5 cm frosted label area is pasted on the front of the bottom box 8, so as to ensure the stability and convenience of the reagent box.
[0045] The use method of the reagent box is as follows: when it is needed to detect and use, first, the foldable handle 3 is folded and placed close to the side of the box body 1, and the middle arch top of the handle, that is, the hand holding part, is placed at the rear side of the box body 1; then, the box cover door 2 at the front side of the box body 1 is opened, so that the rotation angle of the box cover door 2 is greater than 90 degrees; then, the horizontal box 5 is pulled out of the box body 1 along the sliding rails 7 and is horizontally placed on the desktop, the polystyrene hole plate in the rectangular positioning recess 6 mounted on the horizontal box 5 is taken out, and then the horizontal box 5 is inserted into the sliding rails 7 and is placed in the box body 1 along the sliding rails 7; then, the bottom box 8 is pulled out of the box body 1 along the sliding rails 7, according to the sequence from left to right and from top to bottom, the standard sample is first taken out from the circular positioning hole groove 9, and the label colors are red, orange, yellow, green, blue and purple in sequence, then various reagents, such as secondary antibody solution, Eu labeled antibody solution, enhancer, analysis buffer and washing liquid, are taken out from the remaining circular positioning hole grooves 9 in sequence, and the ice bag in the rectangular positioning hole groove 10 is kept unchanged. Finally, the bottom box 8 is inserted into the sliding rails 7 and is placed in the box body 1 along the sliding rails 7, the box cover door 2 is closed, and the foldable handle 3 is vertically lifted.
[0046] The detection operation steps of the reagent box for detecting the PLA2R-IgG antibody are as follows: first, 100 muL of a sample to be detected is added into the coated PLA2R polystyrene hole plate, and is shaken for 1 hour, then, the Tris-HCl washing liquid is used for washing, and the unbound sample is washed away; then, the secondary antibody mouse anti-human IgG is added into the operated polystyrene hole plate for incubation reaction, and the incubation reaction condition is shaking at room temperature for 1 hour; then, the Eu 3+The labeled detection antibody goat anti-mouse IgG is added into the operated polystyrene well plate for incubation reaction, the incubation reaction condition is 1 hour of shaking at room temperature, to obtain the coated antigen-antibody-secondary antibody-Eu 3+ The labeled antibody fluorescence immune complex is obtained; then, the Tris-HCl washing solution is used for washing again; finally, the enhancement solution is added, and after 5 minutes of shaking, the fluorescence intensity is detected by a machine to determine the content of the detection index.
[0047] Example 2
[0048] As Figure 1 , Figure 2 , Figure 3 The utility model relates to a kit for PLA2R-IgG antibody ultra-sensitive detection, including kit main part that is box body 1, the box body 1 is cuboid shape, and the front side of box body 1 is equipped with box cover door 2, and one end of box cover door 2 is hinged (is connected through hinge) with one end of box body 1, and the box body 1 is equipped with foldable handle 3, and the middle position of the left and right sides of box body 1 is fixedly installed with rotary connecting piece 4, and the both ends of foldable handle 3 are rotatably movably connected with box body 1 through the rotary connecting piece 4 on the outer surface of the left and right sides of box body 1, and the inner wall of the left and right sides of box body 1, the upper position and bottom position are fixedly installed with a slide rail 7 with groove respectively, and the groove in the slide rail 7 of upper position is connected with horizontal box 5, and horizontal box 5 is the rectangular flat plate of a certain thickness, and the both sides of horizontal box 5 are inserted into the groove of slide rail 7 and can slide in the groove of slide rail 7, and the upper surface of horizontal box 5 is provided with rectangular positioning recess 6, and rectangular positioning recess 6 is used for installing polystyrene well plate, and the groove in the slide rail 7 of bottom position is connected with bottom box 8, and bottom box 8 is cuboid shape, and the both sides of bottom box 8 are equipped with convex strip, and the convex strip on bottom box 8 is inserted into the groove of slide rail 7, so that bottom box 8 can slide in the groove of slide rail 7, and the upper surface of bottom box 8 is provided with a plurality of circular positioning hole groove 9 and rectangular positioning hole groove 10, and circular positioning hole groove 9 can place reagent bottle of reagent or standard product, and rectangular positioning hole groove 10 can place ice bag.
[0049] The rotary connecting piece 4 comprises a fixed end bearing and a movable end rotary shaft; the box body 1 is provided with mounting holes at the middle positions of the left and right sides; the fixed end bearing of the rotary connecting piece 4 is embedded in the mounting hole (with interference fit), and the left and right rotary connecting pieces 4 are respectively fixedly installed on the outer surfaces of the left and right sides of the box body 1 by embedding the fixed end bearings thereof into the mounting holes (with interference fit); the two ends of the foldable handle 3 are fixedly connected with the movable end rotary shafts of the rotary connecting pieces 4 on the left and right sides of the box body 1, so that the foldable handle 3 can rotate. The two ends of the foldable handle 3 are provided with connecting holes, the two ends of the foldable handle 3 are sleeved on the rotary shafts through the connecting holes, the outer ends of the rotary shafts are provided with threads, and the fixing bolts are installed on the outer ends of the rotary shafts; when the fixing bolts are tightened, the foldable handle 3 and the rotary connecting piece 4 can be fixed, and the foldable handle 3 and the box body 1 can be rotatably connected.
[0050] The box body 1 is integrally formed by high-strength engineering plastic, has a cuboid structure, and has optimal dimensions of 280x160x200mm, which conforms to human engineering; the wall thickness is 3mm after mechanical calculation, and the light weight and pressure resistance are considered.
[0051] The inner wall of the box body 1 is pasted with a polyurethane foam layer, the inside of the box body 1 is pasted with polyurethane foam, the heat preservation and heat insulation effect is excellent, the texture is light and waterproof, and a continuous heat preservation structure is formed.
[0052] The box cover door 2 and the box body 1 are connected through a stainless steel hinge, the hinge is corrosion-resistant and has a long service life. Magnets are arranged in the box cover door 2 and the box body 1, and the opening and closing between the box cover door 2 and the box body 1 is achieved by magnetic adsorption.
[0053] The foldable handle 3 is made of medical-grade ABS plastic and has a smooth surface; the foldable handle 3 has a circular arc shape, the middle arch of the circular arc is a hand holding part, and the two ends of the circular arc are connected to the two sides of the box body 1 through rotary connecting pieces comprising bearings, rotary shafts and fixing bolts; the foldable handle 3 can be rotated and folded by 180 degrees, when it is needed to be carried and used, the foldable handle 3 is lifted to be perpendicular to the box body 1 to be in a vertical state, the middle arch of the handle, i.e. the hand holding part, is located above the box body 1, so that the handle is convenient to hold and carry; when it is not needed to be carried and used, the foldable handle 3 is folded and placed close to the side of the box body 1, the middle arch of the handle, i.e. the hand holding part, is placed at the back side of the box body 1, so that the front side of the box body 1 is not affected to open the door to take and place articles.
[0054] The upper surface of the transverse box 5 is provided with a large rectangular positioning groove 6 for placing polystyrene well plates for detection; the upper surface of the bottom box 8 is provided with a plurality of circular positioning holes 9 of different inner diameters for placing reagent bottles containing reagents or standard samples, and is also provided with a rectangular positioning hole 10 for placing an ice bag; the upper surface of the bottom box 8 is also provided with a rectangular label sticking area subjected to frosted treatment, and the label is used for marking the name of the reagent box, detection items, validity period and the like, so as to facilitate identification and management. The inner wall of the circular positioning hole 9 for placing the reagent bottle containing the reagent or the standard sample is pasted with a 2mm-thick silica gel elastic buffer layer, which functions to fix the reagent bottle and absorb vibration. The thickness of the bottom box 8 is much greater than that of the transverse box 5. The depth of the circular positioning hole 9 and the rectangular positioning hole 10 on the bottom box 8 is much greater than that of the rectangular positioning groove 6 on the transverse box 5.
[0055] The use method and detection operation steps of the reagent box of the embodiment are the same as those of the embodiment 1.
[0056] The reagent box for super-sensitive detection of PLA2R-IgG antibodies in the embodiments 1 and 2 has the following beneficial effects:
[0057] (I) The reagent box is provided with a foldable handle 3 on the side, which is made of ABS plastic and has light weight and high strength.
[0058] (II) The foldable handle 3 is designed to be humanized, and the carrying comfort is improved.
[0059] (III) The foldable handle 3 is connected with the box body 1 through a rotating connecting piece containing a bearing, and the angle can be flexibly adjusted: when carrying, it is vertically lifted, which conforms to ergonomics and uniformly disperses the stress to the palm, reducing the hand compression feeling; when not in use, it can be folded and attached to the side of the box body 1, avoiding occupying additional space and facilitating stacking, transportation or storage.
[0060] (IV) The size of the box body 1 is optimized, which meets the internal component accommodation requirement and controls the overall volume. In combination with the foldable handle 3, the reagent box can be easily put into a container such as a medical kit or a backpack during vehicle transportation, air transportation or manual transportation.
[0061] (V) The transverse box 5 and the bottom box 8 in the reagent box are connected with the box body 1 through the slide rail 7, and can be smoothly and conveniently pulled out for use.
[0062] (Six) the upper surface of the cross box 5 and the bottom box 8 is provided with a plurality of positioning hole slots, such as circular positioning hole slots 9 adapt reagent bottles, rectangular positioning hole slots 6 adapt reaction plate (polystyrene hole plate), rectangular positioning hole slots 10 adapt ice bag; the inner wall of the positioning hole slot is additionally provided with an elastic buffer layer. During transportation, the reagent bottle is tightly wrapped after being inserted into the positioning hole slot, and the elastic buffer layer absorbs the vibration energy, ensuring that the displacement of the components is controlled within a very small range (such as ±1mm). Even if it experiences bumpy road conditions or handling collisions, the internal components can remain stable, avoiding events such as mutual extrusion, dumping or damage.
[0063] It can be seen that the reagent kit for super-sensitive detection of PLA2R-IgG antibody provided by the embodiments 1 and 2 of the utility model has the advantages of reasonable design, optimized structure, perfect function, etc. Through the installation of the sliding rail (7), the cross box (5), the bottom box (8), the positioning hole slot and the foldable handle (3), the convenience and stability during storage and transfer are improved, and the needs for portability and stability of the detection reagent kit in actual detection work can be met.
[0064] The above is only the preferred embodiment of the utility model, and is not used to limit the utility model. For those skilled in the art, the utility model can have various changes and changes, and any modification, equivalent replacement, improvement, etc. within the spirit and principles of the utility model should be included in the protection scope of the utility model.
Claims
1. A kit for ultrasensitive detection of PLA2R-IgG antibodies, comprising a kit body, i.e., a box (1), wherein the box (1) is rectangular; characterized in that: The front of the box body (1) is provided with a lid door (2), one end of which is hinged to one end of the box body (1); the box body (1) is provided with a foldable handle (3); a rotating connector (4) is fixedly installed in the middle of the outer surface of the left and right sides of the box body (1); the two ends of the foldable handle (3) are rotatably connected to the box body (1) through the rotating connector (4); a grooved slide rail (7) is fixedly installed on the inner wall of the left and right sides of the box body (1) at the top and bottom positions respectively; a horizontal box (5) is connected in the groove of the upper slide rail (7), the horizontal box (5) is a rectangular flat plate with a certain thickness, and the two ends of the horizontal box (5) are inserted into the groove of the slide rail (7), and can be placed in the groove of the slide rail (7). The upper surface of the horizontal box (5) is provided with a rectangular positioning groove (6) for placing a polystyrene perforated plate; the bottom box (8) is connected to the groove of the slide rail (7) at the bottom position. The bottom box (8) is rectangular in shape. The two ends of the bottom box (8) are inserted into the groove of the slide rail (7) and the bottom box (8) can slide in the groove of the slide rail (7); the upper surface of the bottom box (8) is provided with several circular positioning slots (9) for placing reagent bottles containing reagents or standards, and also with rectangular positioning slots (10) for placing ice packs; the thickness of the bottom box (8) is greater than the thickness of the horizontal box (5); the depth of the circular positioning slots (9) and the rectangular positioning slots (10) on the bottom box (8) is greater than the depth of the rectangular positioning grooves (6) on the horizontal box (5).
2. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, The rotating connector (4) includes a fixed end bearing and a movable end rotating shaft; mounting holes are provided in the middle of the left and right sides of the box body (1); the fixed end bearing of the rotating connector (4) is embedded in the mounting hole; the two ends of the foldable handle (3) are fixedly connected to the movable end rotating shaft of the rotating connector (4) on the outer surface of the left and right sides of the box body (1), so that rotation can be realized.
3. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 2, characterized in that, The foldable handle (3) has connecting holes at both ends. The two ends of the foldable handle (3) are fitted onto the rotating shaft of the rotating connector (4) through the connecting holes. The outer end of the rotating shaft is threaded, and the fixing bolt is installed on the outer end of the rotating shaft to achieve fixation.
4. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, The box cover (2) is connected to the box body (1) by stainless steel hinges.
5. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, Magnets are provided inside the lid (2) and the box body (1), and the switch between the lid (2) and the box body (1) is opened and closed by magnetic attraction.
6. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 3, characterized in that, The foldable handle (3) is made of medical grade ABS plastic with a smooth surface; the foldable handle (3) is in the shape of an arc arch, the middle arch is the grip part, and the two ends of the arc arch are connected to the two sides of the box body (1) through a rotating connector containing bearings, a rotating shaft and fixing bolts; the foldable handle (3) can be rotated and folded 180 degrees.
7. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, The inner wall of the box (1) is covered with a polyurethane foam insulation layer.
8. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, The box body (1) is made of high-strength engineering plastic in one piece, with a rectangular structure and ergonomic dimensions.
9. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, The upper surface of the bottom box (8) is also provided with a frosted rectangular label pasting area. The label is used to mark the reagent kit name, test items, and expiration date for easy identification and management.
10. The kit for ultrasensitive detection of PLA2R-IgG antibody according to claim 1, characterized in that, A 2-3 mm thick silicone elastic buffer layer is pasted on the inner wall of the circular positioning hole groove (9) of the reagent bottle that can hold reagents or standards.