Treating system for mycoprotein mash

Abstract

The utility model relates to the technical field of treatment of thallus mash, in particular to a treatment system of thallus protein mash. The treatment system comprises a first vacuum concentration system which comprises a mycoprotein mash buffer tank, a first vacuum concentration device and a concentrated protein mash buffer tank, the mycoprotein mash buffer tank is communicated with the first vacuum concentration device, and the first vacuum concentration device is communicated with the concentrated protein mash buffer tank; the concentrated protein mash buffer tank is communicated with the high-pressure homogenizer; the enzymolysis system comprises a batching tank, an alkali liquor feeding pipe and an enzymolysis tank, the batching tank is communicated with the high-pressure homogenizer, the batching tank is communicated with the enzymolysis tank, and the alkali liquor feeding pipe is communicated with the enzymolysis tank; the purification treatment part is communicated with the enzymolysis tank. According to the treatment system, the first vacuum concentration system 1, the high-pressure homogenizer 2, the enzymolysis system 3 and other operation units are combined, so that the content of peptide substances in the mycoprotein mash can be effectively increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of processing of bacterial broth, and particularly relates to a system for processing of bacterial protein broth. BACKGROUND

[0002] At present, unconventional protein resources such as biological bacterial proteins can be used as alternative resources of protein feed. Among them, the technology of producing Clostridium ethanoligenes protein by biological fermentation with carbon-containing gas as raw material not only can realize the effective substitution of protein feed, but also can utilize the carbon-containing industrial tail gas resources, which is an effective measure to practice green and low-carbon development and new-quality production. Existing researches show that the Clostridium ethanoligenes protein has the characteristics of: the weight content of crude protein ≥80%, the amino acid protein ratio is more than 90%, the types of amino acids are complete and the balance is good, and it is easy to be digested and absorbed by animals, etc. Moreover, the bacterial protein has been demonstrated to be safe, effective and environmentally friendly for microorganisms and feed products, and is currently widely used in aquatic, piglet and poultry feed. However, the production process and application of Clostridium ethanoligenes protein have the following problems:

[0003] (1) The water consumption of the production process of Clostridium ethanoligenes protein is large, the use proportion of recycled water in the fermentation system is limited, and the sewage treatment section has a heavy burden. In addition to bacterial proteins, the protein broth after distillation also contains metabolic products such as acetic acid (mass fraction about 5%), phosphoric acid (mass fraction about 0.05%) and lactic acid (mass fraction about 0.01%). Although acetic acid and lactic acid can be used as acidifiers, and phosphoric acid can be used as a phosphorus supplement, these acidifiers and phosphorus supplements can be used in aquatic feed. However, the culture medium after recovering the bacterial protein of the protein broth at the present stage is directly treated as sewage, which undoubtedly causes waste of biological resources;

[0004] (2) The Clostridium ethanoligenes protein cannot be completely recovered and the production energy consumption is high. Since the size of Clostridium ethanoligenes is small (average size is 0.5*3.2 μm) and the mass concentration is low (less than 16 g / L), and at present, the bacterial protein in the bacterial protein broth is mainly recovered by centrifugation and spray drying. However, centrifugation will lose part of the bacterial protein or free amino acids, and in addition, the power consumption and steam consumption of spray drying are high, and the drying cost is high;

[0005] (3) The crushing particle size of Clostridium ethanoligenes protein is too low. Although the bacterial protein product can be improved to a certain extent in the process of spray drying, the crushing particle size of the bacterial protein product after spray drying is still low (90% passes through 300 mesh), which is easy to form dust in the subsequent batching process, and there are safety and health hazards;

[0006] (4) Clostridium ethanoligenes protein has a heavy taste. During the metabolic process of Clostridium ethanoligenes, mixed acids and fusel alcohols are produced, and these mixed acids and fusel alcohols will form special odors in the tail gas during the spray drying process. These special odors will affect the surrounding environment, and the final bacterial protein powder will also have special odors. In the case of high addition amount in animal feed, these special odors will affect the feed intake of animals;

[0007] (5) The processing and nutritional properties of Clostridium ethanoligenes protein cannot be fully released. Clostridium ethanoligenes is a gram-positive bacterium, and its cell wall is mainly composed of peptidoglycan, which has strong mechanical strength and resistance to degradation, limiting the high viscosity properties of Clostridium ethanoligenes protein. In addition, Clostridium ethanoligenes protein contains 6.5% nucleic acid by mass fraction. Although nucleic acid can be directly digested and absorbed by animals, the utilization efficiency of nucleic acid is low. In addition, although Clostridium ethanoligenes protein contains a large amount of bioactive peptides, these bioactive peptides have antioxidant, blood pressure-lowering, and blood sugar-lowering properties. However, due to the lack of directional enzyme system in the animal body, these bioactive peptides cannot effectively exert their own functions.

[0008] In view of the above-mentioned defects of Clostridium ethanoligenes protein, at the present stage, the protein broth containing Clostridium ethanoligenes protein can be subjected to high-efficiency enzymatic reaction treatment to fully release the peptide substances in the protein broth. However, the content of the released peptide substances is low at the present stage, which will cause waste of Clostridium ethanoligenes protein resources.

[0009] At the present stage, the high-efficiency enzymatic reaction treatment technology for protein broth containing Clostridium ethanoligenes protein includes:

[0010] (1) A treatment method and system for industrial tail gas fermentation broth. After homogenization, enzymatic reaction, distillation, and drying treatment of the fermentation broth, small peptide products can be obtained. (2) A treatment method for amino acid fermentation broth. This treatment method uses ultrasonic waves to denature the bacterial cells of the fermentation broth containing amino acids, and then performs solid-liquid separation on the denatured mixture. Utility model content

[0011] The present application provides a system for removing the bad odor of Clostridium bacterial protein, to solve the technical problem of how to simultaneously reduce the content of organic acids and ash in Clostridium bacterial protein.

[0012] In a first aspect, the present application provides a bacterial protein broth treatment system, which comprises:

[0013] The first vacuum concentration system comprises a bacterial protein mash buffer tank, a first vacuum concentration device and a concentrated protein mash buffer tank, the discharge port of the bacterial protein mash buffer tank is communicated with the feed port of the first vacuum concentration device, and the discharge port of the first vacuum concentration device is communicated with the concentrated protein mash buffer tank.

[0014] The high-pressure homogenizer is communicated with the discharge port of the concentrated protein mash buffer tank and the feed port of the high-pressure homogenizer to realize the wall breaking treatment of the concentrated protein mash.

[0015] The enzymolysis system comprises a dosing tank, an alkali liquid feed pipe and an enzymolysis tank, the feed port of the dosing tank is communicated with the discharge port of the high-pressure homogenizer, the discharge port of the dosing tank is communicated with the feed port of the enzymolysis tank, and the discharge port of the alkali liquid feed pipe is communicated with the liquid inlet of the enzymolysis tank, so that the concentrated protein mash is subjected to neutralization reaction and enzymolysis reaction to obtain a protein enzymolysis reaction liquid.

[0016] The purification treatment part is communicated with the discharge port of the enzymolysis tank to purify the protein enzymolysis reaction liquid to obtain a bacterial protein treatment liquid.

[0017] Optionally, the purification treatment part comprises a filter, an electrodialysis system, a second vacuum concentration system and a sterilization device, the feed port of the filter is communicated with the discharge port of the enzymolysis tank, the feed port of the electrodialysis system is communicated with the discharge port of the filter, the feed port of the second vacuum concentration system is communicated with the discharge port of the electrodialysis system, and the feed port of the sterilization device is communicated with the discharge port of the second vacuum concentration system.

[0018] Optionally, the electrodialysis system comprises an electrodialysis device and a desalted liquid temporary storage tank, the feed port of the electrodialysis device is communicated with the discharge port of the filter to subject the protein enzymolysis reaction liquid to desalination treatment to obtain a desalted protein liquid, and the feed port of the desalted liquid temporary storage tank is communicated with the feed port of the electrodialysis device to store the desalted protein liquid.

[0019] Optionally, the second vacuum concentration system comprises a second vacuum concentration device, a gas guide pipe, an adsorption material feed pipe and a desalted protein tank, the feed port of the second vacuum concentration device is communicated with the discharge port of the electrodialysis device, the gas guide pipe is communicated with the gas inlet of the second vacuum concentration device, the adsorption material feed pipe is communicated with the feed port of the second vacuum concentration device, and the feed port of the desalted protein tank is communicated with the discharge port of the second vacuum concentration device; the discharge port of the desalted protein tank is communicated with the sterilization device to subject the desalted protein concentrated liquid to sterilization treatment.

[0020] Optionally, the treatment system further comprises a delivery pump group comprising a fourth delivery pump, and the fourth delivery pump is arranged between the second vacuum concentration device and the desalted liquid temporary storage tank.

[0021] Optionally, the delivery pump group further comprises a first delivery pump, which is arranged between the microbial protein broth buffer tank and the first vacuum concentration device.

[0022] Optionally, the delivery pump group further comprises a second delivery pump, which is arranged between the concentrated protein broth buffer tank and the high-pressure homogenizer.

[0023] Optionally, the delivery pump group further comprises a third delivery pump, which is arranged between the filter and the enzymolysis tank.

[0024] Optionally, the microbial protein broth buffer tank is provided with a first stirring device; and / or

[0025] The concentrated protein broth buffer tank is provided with a second stirring device.

[0026] Optionally, the filter is provided with a microfiltration membrane.

[0027] Compared with the prior art, the above technical solution provided by the embodiments of the present application has the following advantages:

[0028] The processing system for microbial protein broth provided by the present application first enriches the microbial bodies of the microbial protein broth into concentrated protein broth with high microbial body concentration through the first vacuum concentration system comprising a microbial protein broth buffer tank, a first vacuum concentration device and a concentrated protein broth buffer tank, and then performs wall breaking treatment on the concentrated protein broth with high microbial body concentration through a high-pressure homogenizer, so that the microbial bodies of the concentrated protein broth are fully broken, and the microbial proteins and other nutritional ingredients in the microbial bodies are released, and at the same time, the high-pressure homogenizer can also uniformly disperse the microbial proteins and other nutritional ingredients in the concentrated protein broth. The uniformly distributed concentrated protein broth is processed through the enzymolysis system comprising a dosing tank, an alkali liquid feeding pipe and an enzymolysis tank. The concentrated protein broth is first subjected to the alkali liquid feeding pipe to be in an alkaline environment, and the concentrated protein broth in the alkaline environment can promote the microbial proteins to enter the liquid phase system to obtain concentrated protein reaction liquid with high microbial protein content. Then, the concentrated protein reaction liquid is subjected to enzymolysis reaction through the enzymolysis tank, so that most of the microbial proteins are converted into various bioactive peptides. Then, the bioactive peptides and a small amount of microbial proteins generated by the enzymolysis reaction are separated from the concentrated protein reaction liquid through the purification processing unit, so that the microbial protein processing liquid containing a large amount of peptide substances is obtained. Therefore, the processing system combines the operation units such as the first vacuum concentration system, the high-pressure homogenizer and the enzymolysis system, so that the content of peptide substances in the microbial protein broth can be effectively improved. BRIEF DESCRIPTION OF DRAWINGS

[0029] The accompanying drawings, which are incorporated into and form a part of the specification, illustrate preferred embodiments of the present application and, together with the description, serve to explain the principles of the application.

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the accompanying drawings needed to be used in the embodiments or prior art description will be briefly introduced. Obviously, for those skilled in the art, based on the accompanying drawings, other drawings can be obtained without creative labor.

[0031] Figure 1 A schematic diagram of the logical structure of a bacterial protein mash treatment system provided by the embodiments of the present application is shown in the figure.

[0032] Figure 2 A schematic diagram of the detailed logical structure of a bacterial protein mash treatment system provided by the embodiments of the present application is shown in the figure.

[0033] Figure 3 A schematic diagram of the actual structure of a bacterial protein mash treatment system provided by the embodiments of the present application is shown in the figure.

[0034] In the figure, 1 is a first vacuum concentration system, 11 is a bacterial protein mash buffer tank, 12 is a first vacuum concentration device, 13 is a concentrated protein mash buffer tank, 2 is a high-pressure homogenizer, 3 is an enzymatic system, 31 is a batching tank, 32 is an enzymatic tank, 33 is an alkali liquid feeding pipe, 4 is a filter, 5 is an electrodialysis system, 51 is an electrodialysis device, 52 is a desalted liquid temporary storage tank, 6 is a second vacuum concentration system, 61 is a second vacuum concentration device, 62 is a gas guide pipe, 63 is an adsorption material feeding pipe, 64 is a desalted protein tank, 7 is a sterilization device, 8 is a delivery pump set, 81 is a first delivery pump, 82 is a second delivery pump, 83 is a third delivery pump, and 84 is a fourth delivery pump. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solutions, and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0036] Various embodiments of the present application can exist in a range of forms; it should be understood that the description in a range form is merely for the convenience and brevity, and should not be understood as a hard limit to the scope of the present application; therefore, it should be considered that the range described has been specifically disclosed all possible sub-ranges and single values within the range; for example, it should be considered that the range description from 1 to 6 has been specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range; in addition, whenever a numerical range is indicated herein, it refers to any cited number (fraction or integer) within the indicated range.

[0037] In this document, the terms "comprise" and the like are used to mean "include, but not limited to". The terms such as "first" and "second" and the like are merely used to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between the entities or operations. The "and / or", the association relationship of the associated objects, indicates that there can be three relationships, for example, A and / or B, which can represent the cases of A alone, A and B together, and B alone; wherein A and B can be singular or plural. "At least one" means one or more, "multiple" means two or more; "at least one", "at least one of the following" or the like means any combination of the items, including single item or combination of multiple items; for example, "at least one of a, b, or c", or "at least one of a, b, and c", can represent a, b, c, a-b (i.e. a and b), a-c, b-c, or a-b-c, wherein a, b, and c can be single or multiple.

[0038] It should be noted that all directional indications such as up, down, left, right, front, back, etc. in the embodiments of the present application are merely used to explain the relative position relationship between the components, the movement condition, etc. in a certain specific posture (as shown in the drawings), and if the specific posture changes, the directional indications will also change accordingly.

[0039] In the present application, unless otherwise specifically defined and limited, the terms "connection", "fixing", etc. should be understood broadly, for example, "fixing" can be fixed connection, or detachable connection, or integral, which can be mechanical connection, or electrical connection or magnetic connection; can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise specifically limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0040] It should be noted that the reason why the clostridium cell produces an unpleasant odor may be that the production process of clostridium cell protein is a biological fermentation process, and biological fermentation is mainly affected by cell activity, metabolic level and pathway. In the case of unpredictable or unblockable changes in external production conditions, clostridium species will metabolize a high level of organic acids (containing acetic acid and butyric acid), which makes the final cell protein product have a high content of organic acids. These organic acids cause the cell protein to have an unpleasant odor.

[0041] Figure 1 An exemplary schematic diagram of a cell protein mash processing system provided by the present application is shown;

[0042] Figure 3 An exemplary schematic diagram of a cell protein mash processing system provided by the present application is shown as Figure 1 and Figure 3 The present application provides a cell protein mash processing system, which comprises:

[0043] A first vacuum concentration system 1, comprising a cell protein mash buffer tank 11, a first vacuum concentration device 12 and a concentrated protein mash buffer tank 13, wherein the discharge port of the cell protein mash buffer tank 11 is communicated with the feed port of the first vacuum concentration device 12, and the discharge port of the first vacuum concentration device 12 is communicated with the concentrated protein mash buffer tank 13;

[0044] A high-pressure homogenizer 2, wherein the discharge port of the concentrated protein mash buffer tank 13 is communicated with the feed port of the high-pressure homogenizer 2 to realize the wall breaking treatment of the concentrated protein mash;

[0045] An enzymolysis system 3, comprising a dosing tank 31, an alkali feed pipe 33 and an enzymolysis tank 32, wherein the feed port of the dosing tank 31 is communicated with the discharge port of the high-pressure homogenizer 2, the discharge port of the dosing tank 31 is communicated with the feed port of the enzymolysis tank 32, and the discharge port of the alkali feed pipe 33 is communicated with the liquid inlet of the enzymolysis tank 32, so that the concentrated protein mash is subjected to neutralization reaction and enzymolysis reaction to obtain a protein enzymolysis reaction liquid;

[0046] A purification treatment part, which is communicated with the discharge port of the enzymolysis tank 32 to purify and treat the protein enzymolysis reaction liquid to obtain a cell protein treatment liquid.

[0047] It should be noted that the enzyme solution tank 32 generally adds a complex enzyme system including lysozyme, alkaline protease, nuclease and flavor protease, the mass m1 of the lysozyme, the mass m2 of the alkaline protease, the mass m3 of the nuclease and the mass m4 of the flavor protease can satisfy the relationship: m1:m2:m3:m4≥1:1:1:1, this ratio makes the complex enzyme system have sufficient lysozyme, alkaline protease, nuclease and flavor protease, sufficient lysozyme can dissolve the cell wall of Clostridium ethanolium after the broken wall treatment, and act on the β-1,4 glycosidic bond of the peptidoglycan of the cell wall, so that the cell wall of Clostridium ethanolium is disintegrated and the peptidoglycan forms small molecular oligosaccharides, in addition, the disintegrated cell wall of Clostridium ethanolium releases a large amount of bacterial protein and nucleic acid components, and sufficient alkaline protease can destroy the peptide bond of the bacterial protein in the alkaline environment of the protein concentration reaction solution, so that the bacterial protein is decomposed into peptide substances, thereby the concentration of the peptide substances in the enzyme solution reaction solution of the protease can be improved; in addition, sufficient nuclease can divide the nucleic acid molecules into small fragments of nucleotides and nucleosides and other components, which can be quickly absorbed by the body and converted into corresponding nutrients; in addition, sufficient flavor protease can further convert the bacterial protein and part of the peptide substances into amino acids and peptide substances with specific flavors, so as to further optimize the flavor and nutritional value of the bacterial protein treatment solution.

[0048] It should be noted that the lysozyme can use the lysozyme (20,000 U / g) produced by Nanning Dongheng Huadao Biological Technology Co., Ltd.; the alkaline protease can use the alkaline protease (200,000 U / g) produced by Nanning Dongheng Huadao Biological Technology Co., Ltd.; the nuclease can use the nuclease (11,000 U / g) produced by Suzhou Jinan Protein Technology Co., Ltd.; and the flavor protease can use the flavor protease (30,000 U / g) produced by Nanning Dongheng Huadao Biological Technology Co., Ltd.

[0049] It should be noted that the mass m2 of the alkaline protease, the mass m4 of the flavor protease and the dry matter weight m5 of the protein concentration reaction solution satisfy the relationship: (m2+m4) / m5=(1-7):100, so that the protein concentration reaction solution has sufficient alkaline protease and flavor protease, sufficient alkaline protease can destroy the peptide bond of the bacterial protein in the alkaline environment of the protein concentration reaction solution, so that the bacterial protein is decomposed into peptide substances; in addition, sufficient flavor protease can further convert the bacterial protein and part of the peptide substances into amino acids and peptide substances with specific flavors, which can improve the taste and flavor of the subsequent puffed buoyant feed and improve the feeding amount of the meat-eating fish.

[0050] It should be noted that the enzymatic tank 32 also has an enzyme inactivation system, specifically a heating wire is arranged in the enzymatic tank 32 or a heating channel is arranged outside the enzymatic tank 32. Through these structures, the protein enzymatic reaction liquid in the enzymatic tank 32 can be heated to 70-100°C and maintained for 5-10 min, so that the complex enzyme system in the enzymatic tank 32 is inactivated, effectively terminating the progress of the enzymatic reaction, thereby controlling the content of peptide substances in the bacterial protein mash.

[0051] It should be noted that the present application provides a bacterial protein mash processing system. Through the organic combination of multiple operation units, the content of peptide substances in the bacterial protein mash can be significantly improved. Specifically:

[0052] (1) The system first concentrates the bacterial protein mash through the first vacuum concentration system 1 to achieve bacterial enrichment, obtaining a concentrated protein mash with high bacterial concentration. In this step, the bacterial protein mash passes through the bacterial protein mash buffer tank 11 into the first vacuum concentration device 12, and the excess water is removed by vacuum concentration, so that the bacterial concentration is improved. The concentrated protein mash is then temporarily stored in the concentrated protein mash buffer tank 13 to prepare for subsequent processing.

[0053] (2) Then, the concentrated protein mash with high bacterial concentration is subjected to wall breaking treatment by the high-pressure homogenizer 2. The high-pressure action of the high-pressure homogenizer 2 can fully break the bacteria in the concentrated protein mash, so that the bacterial proteins and other nutrients in the bacteria are effectively released. At the same time, the high-pressure homogenizer 2 can also uniformly disperse these nutrients in the concentrated protein mash, providing favorable conditions for subsequent enzymatic reaction.

[0054] (3) Then, the uniformly distributed concentrated protein mash enters the enzymatic system 3 for processing. The enzymatic system 3 includes a dosing tank 31, an alkali feeding pipe 33, and an enzymatic tank 32. First, alkali is added to the concentrated protein mash in the dosing tank 31 through the alkali feeding pipe 33 to make it in an alkaline environment. The alkaline environment is conducive to the entry of bacterial proteins into the liquid phase system, thereby improving the efficiency of enzymatic hydrolysis. Then, the treated concentrated protein mash is sent to the enzymatic tank 32 for enzymatic reaction. Under the action of enzymes, most of the bacterial proteins are converted into various bioactive peptides, which have higher nutritional value and biological activity.

[0055] (4) Finally, the bioactive peptides and a small amount of bacterial proteins generated by the enzymatic reaction are separated from the concentrated protein reaction liquid by the purification treatment part. The purpose of this step is to obtain a bacterial protein processing liquid containing a large amount of peptide substances. The purification treatment part can use appropriate separation and purification techniques to ensure the quality and purity of the final product.

[0056] In summary, the bacterial protein mash treatment system realizes efficient extraction and conversion of bacterial protein through the organic combination of the first vacuum concentration system 1, the high-pressure homogenizer 2, and the enzymatic hydrolysis system 3, and provides strong support for the production of bacterial protein products with high peptide content.

[0057] In some alternative embodiments, the purification treatment part includes a filter 4, an electrodialysis system 5, a second vacuum concentration system 6, and a sterilization device 7; the feed inlet of the filter 4 is communicated with the discharge outlet of the enzymatic hydrolysis tank 32; the feed inlet of the electrodialysis system 5 is communicated with the discharge outlet of the filter 4, the feed inlet of the second vacuum concentration system 6 is communicated with the discharge outlet of the electrodialysis system 5, and the feed inlet of the sterilization device 7 is communicated with the discharge outlet of the second vacuum concentration system 6.

[0058] In these embodiments, the filter 4 is used to filter and remove impurities from the protein enzymatic hydrolysis reaction liquid stored in the enzymatic hydrolysis tank 32, so that large-particle impurities in the protein enzymatic hydrolysis reaction liquid can be removed; the electrodialysis device 51 is used to further remove metal ions such as sodium, magnesium, and iron from the protein filtrate, and then the desalted protein liquid is stored in the desalted liquid temporary storage tank 52; the second vacuum concentration system 6 including the second vacuum concentration device 61, the gas conduit 62, the adsorbent material feeding pipe 63, and the desalted protein material tank 64 is used to introduce composite gas into the second vacuum concentration device 61 through the gas conduit 62, and then introduce adsorbent material into the second vacuum concentration device 61 through the adsorbent material feeding pipe 63, so that the dry matter weight content of the desalted protein liquid after vacuum concentration is more than 50%; finally, the desalted protein concentrate obtained from the second vacuum concentration device 61 is stored in the desalted protein material tank 64 for subsequent processing by the sterilization device 7, so as to finally obtain pure bacterial protein treatment liquid.

[0059] In some alternative embodiments, the electrodialysis system 5 includes an electrodialysis device 51 and a desalted liquid temporary storage tank 52; the feed inlet of the electrodialysis device 51 is communicated with the discharge outlet of the filter 4, so that the protein enzymatic hydrolysis reaction liquid is subjected to desalination treatment to obtain desalted protein liquid; and the feed inlet of the desalted liquid temporary storage tank 52 is communicated with the feed inlet of the electrodialysis device 51, so as to store the desalted protein liquid.

[0060] In these embodiments, the electrodialysis system 5 can include an electrodialysis device 51 and a desalted liquid temporary storage tank 52; the electrodialysis device 51 can be used to remove metal ions such as sodium, magnesium, and iron from the protein filtrate, further reduce the odor of the bacterial protein, and expand the application approach of the bacterial protein treatment liquid; finally, the desalted protein liquid is stored in the desalted liquid temporary storage tank 52 for subsequent processing by the second vacuum concentration system 6.

[0061] It should be noted that the specific use process of the electrodialysis device 51 is as follows: the protein filtrate is placed in the fresh water tank of the electrodialysis device 51, then tap water is placed in the concentrated water tank of the electrodialysis device 51, and sodium chloride brine is placed in the polar water tank of the electrodialysis device 51, the voltage of the electrodialysis device 51 is controlled to be 200V, and the electrodialysis is performed for a time of 60min to 120min according to a cycle period, so as to obtain the desalted protein liquid.

[0062] In some optional embodiments, the second vacuum concentration system 6 comprises a second vacuum concentration device 61, a gas conduit 62, an adsorbent material feeding pipe 63, and a desalted protein material tank 64, the feeding port of the second vacuum concentration device 61 is communicated with the discharging port of the electrodialysis device 51, the gas inlet of the second vacuum concentration device 61 is communicated with the gas conduit 62, the feeding port of the second vacuum concentration device 61 is communicated with the adsorbent material feeding pipe 63, and the discharging port of the second vacuum concentration device 61 is communicated with the feeding port of the desalted protein material tank 64; the discharging port of the desalted protein material tank 64 is communicated with the sterilization device 7, so as to perform sterilization treatment on the desalted protein concentrate.

[0063] In these embodiments, the second vacuum concentration system 6 comprising the second vacuum concentration device 61, the gas conduit 62, the adsorbent material feeding pipe 63, and the desalted protein material tank 64, the composite gas can be introduced into the second vacuum concentration device 61 by the gas conduit 62, and the adsorbent material can be introduced by the adsorbent material feeding pipe 63, during the working stage of the second vacuum concentration device 61, the continuously introduced composite gas can carry out the abnormal odor generated in the vacuum concentration stage, and the dry matter weight content of the desalted protein liquid after vacuum concentration is more than 50%.

[0064] It should be noted that the composite gas can comprise carbon dioxide and / or nitrogen; in the case that the composite gas comprises carbon dioxide, the input flow rate of the carbon dioxide can be 1% to 5% of the total flow rate of the composite gas; in the case that the composite gas comprises nitrogen, the input flow rate of the nitrogen can be 1% to 5% of the total flow rate of the composite gas, so that there is sufficient carbon dioxide and nitrogen in the composite gas, thereby effectively carrying out the abnormal odor generated in the vacuum concentration stage.

[0065] It should be noted that the adsorbent material can be activated carbon, and the mass ratio of the adsorbent material to the desalted protein liquid can be greater than or equal to 1:100, so that the desalted protein liquid has sufficient adsorbent material, the sufficient adsorbent material can effectively enrich the bacterial protein, the peptide substance, the oligosaccharide, the nucleotide, and the nucleoside and other small molecule substances, avoid the composite gas from carrying out these small molecule substances, and make the dry matter weight content of the desalted protein liquid after vacuum concentration be more than 50%.

[0066] Figure 3An actual structure schematic diagram of a bacteria protein mash processing system provided by the embodiment of the present application is exemplarily shown;

[0067] In some optional embodiments, as shown in Figure 3 The processing system further comprises a delivery pump set 8 including a fourth delivery pump 84, which is arranged between the second vacuum concentration device 61 and the desalted liquid temporary storage tank 52.

[0068] In these embodiments, the introduction of the delivery pump set 8 including the fourth delivery pump 84 in the processing system can completely transfer the desalted protein liquid obtained by the electrodialysis device 51 to the second vacuum concentration device 61 for vacuum concentration, so as to realize the rapid transfer and processing of the desalted protein liquid, thereby improving the processing efficiency of the overall processing system and increasing the content of the peptide substances in the bacteria protein processing liquid.

[0069] In some optional embodiments, the delivery pump set 8 further comprises a first delivery pump 81, which is arranged between the bacteria protein mash buffer tank 11 and the first vacuum concentration device 12.

[0070] In these embodiments, the introduction of the first delivery pump 81 in the delivery pump set 8 can rapidly transfer the raw material of the bacteria protein mash in the bacteria protein mash buffer tank 11 to the first vacuum concentration device 12 for vacuum concentration, thereby improving the processing efficiency of the overall processing system and increasing the content of the peptide substances in the bacteria protein processing liquid.

[0071] In some optional embodiments, the delivery pump set 8 further comprises a second delivery pump 82, which is arranged between the concentrated protein mash buffer tank 13 and the high-pressure homogenizer 2.

[0072] In these embodiments, the introduction of the second delivery pump 82 in the delivery pump set 8 can completely transfer the concentrated protein mash in the concentrated protein mash buffer tank 13 to the high-pressure homogenizer 2 for wall-breaking processing, thereby improving the processing efficiency of the overall processing system and increasing the content of the peptide substances in the bacteria protein processing liquid.

[0073] In some optional embodiments, the delivery pump set 8 further comprises a third delivery pump 83, which is arranged between the filter 4 and the enzymolysis tank 32.

[0074] In these embodiments, the introduction of the third delivery pump 83 in the delivery pump set 8 can completely transfer the protease enzymolysis reaction liquid in the enzymolysis tank 32 to the filter 4 for filtration processing, thereby improving the processing efficiency of the overall processing system and increasing the content of the peptide substances in the bacteria protein processing liquid.

[0075] In some alternative embodiments, the bacteria protein broth buffer tank 11 is provided with a first stirring device 111; and / or

[0076] The concentrated protein broth buffer tank 13 is provided with a second stirring device 131.

[0077] In these embodiments, the introduction of the first stirring device 111 in the bacteria protein broth buffer tank 11 can make the various substances in the raw material of the bacteria protein broth fully mixed; in addition, the introduction of the second stirring device 131 in the concentrated protein broth buffer tank 13 can promote the uniform mixing of the concentrated protein broth, facilitating the subsequent enzymatic reaction of the enzymatic system 3.

[0078] In some alternative embodiments, the filter 4 is provided with microfiltration membranes.

[0079] In these embodiments, the filter 4 is provided with microfiltration membranes, which can effectively remove large-particle impurities from the protease reaction solution, ensuring the purity of small-molecule substances such as bacterial proteins, peptides, oligosaccharides, nucleotides, and nucleosides.

[0080] In summary, the present application provides a bacteria protein broth processing system, which first enriches the bacteria of the bacteria protein broth into concentrated protein broth with high bacteria concentration through the first vacuum concentration system 1 comprising the bacteria protein broth buffer tank 11, the first vacuum concentration device 12, and the concentrated protein broth buffer tank 13, then performs wall breaking treatment on the concentrated protein broth with high bacteria concentration through the high-pressure homogenizer 2, which can fully break the bacteria of the concentrated protein broth, so that the bacterial proteins and other nutritional ingredients in the bacteria are released, and the high-pressure homogenizer 2 can also uniformly disperse the bacterial proteins and other nutritional ingredients in the concentrated protein broth. The uniformly distributed concentrated protein broth is then processed by the enzymatic system 3 comprising the dosing tank 31, the alkali liquid feeding pipe 33, and the enzymatic tank 32. The concentrated protein broth is first subjected to an alkaline environment through the alkali liquid feeding pipe 33, which can promote the bacterial proteins to enter the liquid phase system to obtain concentrated protein reaction liquid with high bacterial protein content. Then, the concentrated protein reaction liquid is subjected to enzymatic reaction through the enzymatic tank 32, which can convert most of the bacterial proteins into various bioactive peptides. Finally, the bioactive peptides and a small amount of bacterial proteins generated by the enzymatic reaction are separated from the concentrated protein reaction liquid through the purification processing unit, thereby obtaining bacteria protein processing liquid containing a large amount of peptide substances. Therefore, the combination of the first vacuum concentration system 1, the high-pressure homogenizer 2, and the enzymatic system 3 can effectively improve the content of peptide substances in the bacteria protein broth.

[0081] In addition, the application provides a processing system for the bacterial protein mash, which introduces an alkali liquor feeding pipe 33 in the enzymolysis system 3, can convert the miscellaneous acids such as acetic acid, phosphoric acid and lactic acid in the bacterial protein mash into salts, realizes the high-value utilization of the miscellaneous acids, eliminates the peculiar smell of the miscellaneous acids, and through the directional enzymolysis treatment of the enzymolysis system 3, can fully enzymolyze the bacterial protein in the bacterial protein mash to form peptide substances, so as to release the processing characteristics and nutritional characteristics of Clostridium ethanolium protein.

[0082] In addition, the application provides a processing system for the bacterial protein mash, compared with the traditional bacterial protein processing system, can save the use of a large number of centrifugal and spray drying devices, so as to reduce the risk of losing bacterial protein due to centrifugation or spray drying stage, reduce the energy consumption of bacterial protein production, and reduce the sewage discharge amount of bacterial protein processing, so that the bacterial protein mash can be directly supplied to the downstream feed producer in the form of liquid enzymolysis liquid.

[0083] The above description is only a specific embodiment of the application, which enables those skilled in the art to understand or implement the application. Various modifications of these embodiments will be apparent to those skilled in the art, and the general principles defined in the application can be implemented in other embodiments without departing from the spirit or scope of the application. Therefore, the application will not be limited to these embodiments shown in the application, but will conform to the widest scope consistent with the principles and novel features of the application claimed.

Claims

1. A system for processing bacterial protein mash, characterized in that, The processing system includes: The first vacuum concentration system (1) includes a microbial protein mash buffer tank (11), a first vacuum concentration device (12), and a concentrated protein mash buffer tank (13). The outlet of the microbial protein mash buffer tank (11) is connected to the inlet of the first vacuum concentration device (12), and the outlet of the first vacuum concentration device (12) is connected to the concentrated protein mash buffer tank (13). The outlet of the high-pressure homogenizer (2) is connected to the inlet of the high-pressure homogenizer (2) to achieve the cell wall breaking treatment of the concentrated protein mash. The enzymatic hydrolysis system (3) includes a mixing tank (31), an alkali feed pipe (33), and an enzymatic hydrolysis tank (32). The feed inlet of the mixing tank (31) is connected to the discharge outlet of the high-pressure homogenizer (2), the discharge outlet of the mixing tank (31) is connected to the feed inlet of the enzymatic hydrolysis tank (32), and the discharge outlet of the alkali feed pipe (33) is connected to the liquid inlet of the enzymatic hydrolysis tank (32), so that the concentrated protein mash undergoes a neutralization reaction and an enzymatic hydrolysis reaction to obtain a protein hydrolysis reaction solution. The purification treatment unit is connected to the outlet of the enzymatic hydrolysis tank (32) to purify the enzymatic hydrolysis reaction solution and obtain the bacterial protein treatment solution.

2. The processing system according to claim 1, characterized in that, The purification process includes: a filter (4), an electrodialysis system (5), a second vacuum concentration system (6), and a sterilization device (7); the inlet of the filter (4) is connected to the outlet of the enzymatic hydrolysis tank (32); the inlet of the electrodialysis system (5) is connected to the outlet of the filter (4); the inlet of the second vacuum concentration system (6) is connected to the outlet of the electrodialysis system (5); and the inlet of the sterilization device (7) is connected to the outlet of the second vacuum concentration system (6).

3. The processing system according to claim 2, characterized in that, The electrodialysis system (5) includes an electrodialysis device (51) and a desalination solution storage tank (52). The inlet of the electrodialysis device (51) is connected to the outlet of the filter (4) so ​​that the proteolytic reaction solution is desalted to obtain a desalted protein solution. The inlet of the desalination solution storage tank (52) is connected to the inlet of the electrodialysis device (51) to store the desalted protein solution.

4. The processing system according to claim 3, characterized in that, The second vacuum concentration system (6) includes a second vacuum concentration device (61), a gas conduit (62), an adsorbent material feed pipe (63), and a desalted protein tank (64). The inlet of the second vacuum concentration device (61) is connected to the outlet of the electrodialysis device (51). The gas conduit (62) is connected to the air inlet of the second vacuum concentration device (61). The adsorbent material feed pipe (63) is connected to the inlet of the second vacuum concentration device (61). The inlet of the desalted protein tank (64) is connected to the outlet of the second vacuum concentration device (61). The outlet of the desalted protein tank (64) is connected to the sterilization device (7) to sterilize the desalted protein concentrate.

5. The processing system according to claim 4, characterized in that, The processing system further includes a transfer pump group (8), including a fourth transfer pump (84), which is located between the second vacuum concentration device (61) and the desalination liquid storage tank (52).

6. The processing system according to claim 5, characterized in that, The pump assembly (8) further includes a first pump (81), which is located between the bacterial protein mash buffer tank (11) and the first vacuum concentration device (12).

7. The processing system according to claim 5, characterized in that, The pump assembly (8) further includes a second pump (82), which is located between the concentrated protein mash buffer tank (13) and the high-pressure homogenizer (2).

8. The processing system according to claim 5, characterized in that, The pump assembly also includes a third pump (83), which is located between the filter (4) and the enzymatic hydrolysis tank (32).

9. The processing system according to claim 1, characterized in that, The bacterial protein mash buffer tank (11) is equipped with a first stirring device (111); and / or The concentrated protein mash buffer tank (13) is equipped with a second stirring device (131).

10. The processing system according to claim 2, characterized in that, The filter (4) is equipped with a microfiltration membrane.

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