An evaporation-resistant reaction plate device for PCR reaction

Abstract

The application relates to the field of biological experimental consumables, and discloses an evaporation-preventing reaction plate device for PCR (Polymerase Chain Reaction) reaction. In the application, the PCR reaction plate body is provided with a top cover layer, a heating layer is fixedly arranged in the middle of the top cover layer, a heat cover is fixedly arranged at the top of the heating layer, and a silica gel sealing ring is arranged at the periphery of the bottom of the heat cover. A heating device is arranged at the top of the PCR reaction device, the heating device heats the heat cover to about 105 DEG C, the temperature of the heat cover is higher than that of the reaction liquid, the reaction liquid is effectively prevented from evaporating, the sealing property of the reaction tube is further improved through the silica gel sealing ring at the bottom of the heat cover, each heat cover can be used independently, the heat cover can be directly taken out by buckling from the top, a dust shield is arranged at the top to effectively intercept dust, the reaction device is convenient to use normally next time, the reaction plate at the bottom and the reaction tube groove are integrally quickly detachable and installable, and are convenient to clean and replace.

Description

Technical Field

[0001] This application belongs to the field of biological experimental consumables technology, specifically an anti-evaporation reaction plate device for PCR reactions. Background Technology

[0002] PCR, or polymerase chain reaction, is a commonly used technique in modern molecular biology research and clinical medical diagnosis. PCR is a method for rapidly amplifying specific genes or DNA sequences in vitro; it is also known as in vitro gene amplification. PCR is a molecular biology technique used to amplify specific DNA fragments; it can be viewed as a special form of DNA replication outside of a living organism. The most significant characteristic of PCR is its ability to dramatically increase minute amounts of DNA. After several cycles of denaturation, annealing, and extension using two oligonucleotide primers flanking and complementary to the DNA fragment to be amplified, the DNA is amplified by a power of 2. However, the thermal cycling process in experiments can lead to significant liquid evaporation, which can cause inaccurate results. Therefore, PCR reaction devices with anti-evaporation features were developed.

[0003] For example, CN206666509U discloses a PCR reaction plate, including a workbench, a shaping frame, and a base. The workbench and shaping frame are disposed on the base. The workbench is disposed inside the shaping frame. The workbench includes a connecting plate and a reaction tube. The connecting plate is connected to the shaping frame. The upper end of the reaction tube is open and connected to the connecting plate, and the lower end of the reaction tube is closed. The opening of the reaction tube protrudes from the connecting plate to form a sealing ring. A heat-sealing film is separately disposed from the workbench, and the heat-sealing film is thermally fused with the sealing ring to seal the reaction tube. The PCR reaction plate provided by this utility model has excellent sealing effect, avoiding the evaporation of reaction reagents during thermal cycling, thereby ensuring the accuracy of experimental results.

[0004] However, common PCR reaction plates only seal the reaction solution, resulting in insufficient overall evaporation protection. During thermal cycling, the large amount of liquid evaporation can easily lead to inaccurate experimental results, requiring further improvement. Utility Model Content

[0005] The purpose of this application is to provide an anti-evaporation reaction plate device for PCR reactions, in order to address the problem that the overall anti-evaporation performance of the aforementioned device is not perfect, the amount of liquid evaporation during thermal cycling is large, and the experimental results are easily inaccurate, which requires further improvement.

[0006] The technical solution adopted in this application is as follows: An anti-evaporation reaction plate device for PCR reaction includes a PCR reaction plate body, a top cover layer is provided on the top of the PCR reaction plate body, a heating layer is fixedly installed in the middle of the top cover layer, a heat cover is fixed on the top of the heating layer, a heat cover top is provided on the top of the heat cover, a heat cover connecting post is fixedly connected below the heat cover top, a heat cover bottom is fixedly connected to the bottom of the heat cover connecting post, and a silicone sealing ring is provided around the bottom of the heat cover.

[0007] By adopting the above technical solution, a heating layer is set at the top of the PCR reaction device, and a heating device is set in the heating layer. The heating device heats the hot cap to about 105 degrees Celsius. By heating the hot cap to a temperature higher than that of the reaction solution, the reaction solution is less likely to evaporate. The silicone sealing ring at the bottom of the hot cap further increases the sealing of the reaction tube. Because the hot cap is hollow in the middle and connected to the bottom through a hot cap connecting post, each hot cap can be used independently, making it easy to disassemble and replace. When replacement is needed, the hot cap can be removed directly from the top.

[0008] In a preferred embodiment, a dustproof plate fixing block is fixedly installed on the top of the top cover layer, and a fixing handle is rotatably connected to the outer side of the dustproof plate fixing block.

[0009] By adopting the above technical solution, a mounting block is fixed on the top of the heating layer, and a fixing handle is installed on the other side of the mounting block, thereby facilitating the movement of the dust cover.

[0010] In a preferred embodiment, the fixed handle is fixedly connected to the rotating handle.

[0011] By adopting the above technical solution, a rotating handle is installed on the fixed handle, thereby driving the rotation function.

[0012] In a preferred embodiment, the rotating handle is rotatably connected to a rotating connector, and the rotating connector is fixedly connected to a dust cover.

[0013] By adopting the above technical solution, the dust cover can be fixed on the rotating connector. Under normal storage conditions, the dust cover function can be used. When the reaction plate device function is needed, the dust cover can be reversed to the back. The dust cover can also be easily replaced and disassembled when maintenance and cleaning are required.

[0014] In a preferred embodiment, the bottom of the top cover layer is fixedly connected to a reaction tank shell, and a sliding groove is provided on the side of the reaction tank shell.

[0015] By adopting the above technical solution, the outer shell of the reaction tube tank is fixed below the top cover layer, and a slide is set on the outer shell. By sliding the fixing handle and fixing block, it is helpful for the normal use and storage of the reaction plate.

[0016] In a preferred embodiment, a fixed base is fixedly installed at the bottom of the top cover layer.

[0017] By adopting the above technical solution and installing a fixed base at the bottom of the reaction plate, the overall stability of the PCR anti-evaporation device is improved.

[0018] In a preferred embodiment, the fixed base is slidably connected to a fixed block.

[0019] By adopting the above technical solution, the reaction plate is used and stored by sliding along the slide rail. The reaction plate can be disassembled separately, making it convenient to disassemble when maintenance and cleaning are required.

[0020] In a preferred embodiment, a reaction plate is slidably connected to the other side of the reaction tube tank shell, the reaction plate is provided with a reaction tube tank, and a reaction tube is placed inside the reaction tube tank.

[0021] By adopting the above technical solution, a reaction tube groove is set in the reaction plate. First, slide the reaction plate to the usable position, then pour the reaction liquid into the reaction tube, or directly place the prepared reaction tube in the groove, fix the position, and then press the corresponding hot cap according to the position of the reaction tube to complete the overall sealing of the reaction tube.

[0022] In summary, due to the adoption of the above technical solution, the beneficial effects of this application are:

[0023] In this application, a heating layer is set at the top of the PCR reaction device, and a heating device is set in the middle of the heating cover layer. The heating device heats the temperature of the heating cover to about 105 degrees Celsius, making the temperature of the heating cover higher than the temperature of the reaction liquid, thereby reducing the evaporation of the reaction liquid, thus ensuring normal experimentation and the accuracy of experimental results. Then, the rubber sealing ring at the bottom of the heating cover is used to further enhance the sealing of the reaction tube and further reduce the possibility of evaporation. Each heating cover can be used independently, which is convenient for disassembly and replacement. When replacement is needed, the heating cover can be removed directly from the top. A dust shield is set at the top to help the reaction device effectively intercept dust when not in use and facilitate normal use next time. The reaction plate and reaction tube tank at the bottom can also be quickly disassembled and installed, which is convenient for cleaning and replacement. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall PCR reaction plate of this application;

[0025] Figure 2 This is a schematic diagram of the unfolded reaction plate in this application;

[0026] Figure 3 This is a schematic diagram of the heating layer structure in this application;

[0027] Figure 4 This is a schematic diagram of the heat-cap structure in this application;

[0028] Figure 5 This is a schematic diagram of the reaction tank structure in this application.

[0029] The diagram shows the following components: 1. PCR reaction plate body; 2. Top cover layer; 3. Heating layer; 4. Heat cover; 5. Fixing handle; 6. Dust shield; 7. Reaction tube holder shell; 8. Sliding groove; 9. Fixing base; 10. Fixing block; 11. Reaction plate; 12. Reaction tube holder; 13. Dust shield fixing block; 14. Rotating handle; 15. Rotating connector; 16. Top of heat cover; 17. Heat cover connecting post; 18. Bottom of heat cover; 19. Silicone sealing ring; 20. Reaction tube. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below in conjunction with the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0031] Reference Figure 1-5 ,

[0032] Example:

[0033] An anti-evaporation reaction plate device for PCR reaction includes a PCR reaction plate body 1, a top cover layer 2 on the top of the PCR reaction plate body 1, and a heating layer 3 fixedly installed in the middle of the top cover layer 2. By setting a heating layer on the top of the PCR reaction device and setting a heating device in the heating layer, the heating device heats the hot cover to about 105 degrees Celsius. By heating the hot cover to a temperature higher than that of the reaction solution, the reaction solution is effectively prevented from evaporating.

[0034] A heat cover 4 is fixed to the top of the heating layer 3. A heat cover top 16 is provided on the top of the heat cover 4. A heat cover connecting post 17 is fixedly connected to the bottom of the heat cover top 16. A heat cover bottom 18 is fixedly connected to the bottom of the heat cover connecting post 17. A silicone sealing ring 19 is provided around the bottom of the heat cover. The silicone sealing ring at the bottom of the heat cover further increases the sealing of the reaction tube. The middle of the heat cover is hollow and connected to the bottom through the heat cover connecting post, ensuring that each heat cover can be used independently and is easy to disassemble and replace. When replacement is needed, the heat cover can be taken out directly from the top.

[0035] A dustproof plate fixing block 13 is fixedly installed on the top of the top cover layer 2. A fixing handle 5 is rotatably connected to the outside of the dustproof plate fixing block 13. By fixing the mounting block on the top of the heating layer and installing the fixing handle on the other side of the mounting block, the dustproof plate can be moved.

[0036] The fixed handle 5 is fixedly connected to the rotating handle 14. By installing the rotating handle on the fixed handle, the rotation function is driven.

[0037] The rotating handle 14 is rotatably connected to the rotating connector 15, and the rotating connector 15 is fixedly connected to the dust cover 6. By fixing the dust cover to the rotating connector, the dust cover function can be used when the device is normally stored. When the reaction plate device function needs to be used, the dust cover can be reversed to the back. The dust cover can be easily replaced and disassembled when maintenance and cleaning are required.

[0038] The bottom of the top cover layer 2 is fixedly connected to the outer shell 7 of the reaction tube tank. A sliding groove 8 is provided on the side of the outer shell 7 of the reaction tube tank. The outer shell of the reaction tube tank is fixed below the top cover layer. A slide is provided on the outer shell. By sliding the fixing handle and fixing block, it is helpful for the normal use and storage of the reaction plate.

[0039] A fixing base 9 is fixedly installed at the bottom of the top cover layer 2. The overall stability of the PCR anti-evaporation device is improved by installing the fixing base at the bottom of the reaction plate.

[0040] The fixed base 9 is slidably connected to the fixed block 10, which slides through the slide rail to be used for storing the reaction plate, and can be easily disassembled when maintenance and cleaning are required.

[0041] A reaction plate 11 is slidably connected to the other side of the reaction tube tank shell 7. The reaction plate 11 is provided with a reaction tube tank 12. The reaction tube 20 is placed inside the reaction tube tank 12. By providing a reaction tube tank inside the reaction plate, the reaction plate is first slid to a usable position, and then the reaction liquid is poured into the reaction tube, or the prepared reaction tube is directly placed in the tank, the position is fixed, and then the corresponding hot cap is pressed according to the position of the reaction tube to complete the overall sealing of the reaction tube.

[0042] The implementation principle of the anti-evaporation reaction plate device for PCR reaction in this application is as follows: A top cover layer 2 is provided on the top of the PCR reaction plate body 1. A heating layer 3 is fixedly installed in the middle of the top cover layer 2. A hot cover 4 is fixed on the top of the heating layer 3. A hot cover top 16 is provided on the top of the hot cover 4. A hot cover connecting post 17 is fixedly connected below the hot cover top 16. A hot cover bottom 18 is fixedly connected to the bottom of the hot cover connecting post 17. A silicone sealing ring 19 is provided around the hot cover bottom 18. By providing a heating device on the top of the PCR reaction device, the hot cover is heated to about 105 degrees Celsius. By heating the hot cover to a temperature higher than that of the reaction liquid, the reaction liquid is effectively prevented from evaporating. The silicone sealing ring at the bottom of the hot cover further increases the sealing of the reaction tube.

[0043] The above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. An evaporation-proof reaction plate device for PCR reaction, comprising a PCR reaction plate main body (1), characterized in that: The PCR reaction plate body (1) has a top cover layer (2) on top, a heating layer (3) is fixedly installed in the middle of the top cover layer (2), a heat cover (4) is fixed on the top of the heating layer (3), a heat cover top (16) is provided on the top of the heat cover (4), a heat cover connecting post (17) is fixedly connected below the heat cover top (16), a heat cover bottom (18) is fixedly connected to the bottom of the heat cover connecting post (17), and a silicone sealing ring (19) is provided around the heat cover bottom (18).

2. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 1, wherein: A dustproof plate fixing block (13) is fixedly installed on the top of the top cover layer (2), and a fixing handle (5) is rotatably connected to the outer side of the dustproof plate fixing block (13).

3. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 2, wherein: The fixed handle (5) is fixedly connected to the rotating handle (14).

4. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 3, wherein: The rotating handle (14) is rotatably connected to a rotating connector (15), and the rotating connector (15) is fixedly connected to a dust cover (6).

5. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 1, wherein: The bottom of the top cover layer (2) is fixedly connected to the reaction tank shell (7), and a sliding groove (8) is provided on the side of the reaction tank shell (7).

6. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 1, wherein: The bottom of the top cover layer (2) is fixedly installed with a fixed base (9).

7. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 6, wherein: The fixed base (9) is slidably connected to a fixed block (10).

8. An evaporation resistant reaction plate apparatus for PCR reactions as defined in claim 5, wherein: A reaction plate (11) is slidably connected to the other side of the outer shell (7) of the reaction tube tank. A reaction tube tank (12) is provided on the reaction plate (11), and a reaction tube (20) is placed inside the reaction tube tank (12).

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