A rotary seal pipette reactor

Abstract

The utility model relates to the field of biological medicine experiment technology, concretely is a kind of reactor of rotary sealed pipetting, including the rotary cover for being inserted with syringe body and the sealing cover for being detachably connected with reagent bottle body, the rotary cover is sleeved in the sealing cover far from the one end of reagent bottle body far from syringe body, and the upper cavity of sealing cover is provided with annular sealing washer between sealing cover and rotary cover, the surface of annular sealing washer is provided with a plurality of sealing washer through holes. The utility model can form a stable conduction structure capable of switching by rotation by the cooperation of rotary cover, sealing cover, annular sealing washer and fixing nut;And the structure can make reagent bottle body and external container communicate, then combine commercial syringe body, ensure the accuracy of pipetting by using own capacity scale, then realize the accurate injection or suction of liquid, finally achieve small volume closed pipetting.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental technology, specifically to a rotary sealed liquid transfer reactor. Background Technology

[0002] In the field of biomedical experiments, the transfer of reagents between containers (i.e., pipetting) is one of the core steps in the experimental procedure. The airtightness, volume accuracy, and cost control of this operation directly affect the reliability of experimental results and the economy of the experimental protocol. Currently, the three common pipetting methods in the industry are manual pouring, syringe pump pipettes, and peristaltic pump air displacement pipetting modules.

[0003] Among these methods, manual pouring is a completely open operation, which not only makes it impossible to precisely control the volume of liquid being pipetted, but also easily leads to reagent waste or experimental deviations due to operational errors. At the same time, the reagents are directly exposed to the environment, posing a risk of contamination. Although the syringe pump pipette can achieve precise control of the volume of liquid being pipetted through its mechanical structure, it is still an open pipetting system. The reagents come into direct contact with the air during the transfer process, making them susceptible to the influence of environmental contaminants such as bacteria and dust, which can lead to reagent deterioration or distorted experimental results, failing to meet the requirements of a sterile environment for biomedical experiments. While the peristaltic pump air displacement pipetting module can achieve closed pipetting and avoid reagent contamination, its core components have inherent defects: on the one hand, long-term use of the motor drive and silicone tubing can easily lead to tubing wear, directly causing unstable pipetting volumes, requiring additional sensors for real-time correction, increasing the complexity and cost of the equipment; on the other hand, due to its structural principle, this module cannot meet the small-volume pipetting needs commonly encountered in biomedical experiments.

[0004] Currently, microfluidics are commonly used for closed-loop pipetting of small volumes. However, microfluidics typically employs customized chemical etching processes, which are not only time-consuming but also expensive to customize, hindering their widespread adoption in conventional laboratories or small and medium-sized enterprises. Therefore, there is a need to propose a low-cost, accurate closed-loop reactor. Utility Model Content

[0005] This invention provides a rotary sealed liquid transfer reactor to solve the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows: A rotary sealed pipetting reactor includes a rotating cap for insertion into a syringe body and a sealing cap for detachable connection to a reagent bottle body. The end of the rotating cap away from the syringe body is fitted onto the end of the sealing cap away from the reagent bottle body. An annular sealing gasket is disposed within the upper cavity of the sealing cap between the sealing cap and the rotating cap. Multiple through holes are formed on the surface of the annular sealing gasket. A needle hole corresponding to one of the through holes is formed on the rotating cap. At least one first pipetting hole and at least one second pipetting hole are formed on the sealing cap. All ports are configured to correspond to the through holes of the sealing gasket, and the sum of the number of the first pipetting hole and the second pipetting hole is equal to the number of the through holes of the sealing gasket. The other port of the first pipetting hole is located in the lower cavity of the sealing cap, and the other port of the second pipetting hole is located on the circumferential wall of the sealing cap. The other port of the second pipetting hole is provided with a perforated connector. A central component is integrally formed in the central area of ​​the sealing cap. A central hole is provided through the central component along the axial direction of the sealing cap. One end of the central component penetrates the bottom of the rotating cap and extends into the inner cavity of the rotating cap. A fastener is detachably connected to the end of the central component located in the inner cavity of the rotating cap.

[0007] Preferably, the fastener includes a nut washer and a fixing nut. The nut washer is used to be sleeved on one end of the central member, and the fixing nut is used to be threaded to one end of the central member. The outer wall of one end of the central member is provided with a first external thread section for connecting with the fixing nut, and the inner wall of one end of the central member is provided with a first internal thread section. The first internal thread section and the first external thread section are located at the same end of the central member.

[0008] Preferably, the outer wall of one end of the first pipette hole is provided with an anti-slip protrusion, the inner wall of one end of the second pipette hole is provided with a second internal thread section, and the first pipette hole and the second pipette hole are distributed at intervals along the circumferential direction on the sealing cap.

[0009] Preferably, the sealing gasket has n through holes, and n≥2. When n is even, the n sealing gasket through holes are axially symmetrically distributed with respect to the central axis of the annular sealing gasket. When n is odd, the n sealing gasket through holes are axially symmetrically distributed or asymmetrically distributed along the circumference.

[0010] Preferably, the rotating cover has an annular groove at one end near the sealing cover, the inner wall of the annular groove has at least one first sealing ring groove for placing the sealing ring, and the inner cavity of the rotating cover has at least one second sealing ring groove for placing the sealing ring.

[0011] Preferably, one side of the needle tube hole is provided with a through hole opened on the rotating cover, one end of the central member is used to be disposed inside the through hole, and the inner wall of the through hole is provided with at least one third sealing ring groove for placing the sealing ring.

[0012] Preferably, the outer wall of the rotating cover is provided with teeth along the circumferential direction, and one end of each tooth is provided with a partition area. The outer wall of the rotating cover is provided with a vertical column corresponding to the partition area, and the central longitudinal section of the vertical column along the length direction is on the same plane as the center of the needle hole.

[0013] Preferably, the sealing cap is provided with at least one first positioning groove and at least one second positioning groove along the circumferential direction. The number of first positioning grooves is equal to the number of first pipetting holes, and the number of second positioning grooves is equal to the number of second pipetting holes. The first positioning grooves are arranged in a one-to-one correspondence with the first pipetting holes, and the second positioning grooves are arranged in a one-to-one correspondence with the second pipetting holes.

[0014] Preferably, the perforated connector has a through hole extending along the axial direction, and the outer wall of the perforated connector has a second external thread section and an anti-slip texture section, wherein the second external thread section is used to thread-connect with the second internal thread section of the inner wall of the second pipetting hole.

[0015] Preferably, the outer wall of the sealing cap is provided with an anti-slip groove along the circumferential direction, and the inner wall of the lower cavity of the sealing cap is provided with a third internal thread section, and the sealing cap is connected to the reagent bottle body through the third internal thread section.

[0016] By adopting the above technical solution, the beneficial effects achieved by this utility model are as follows: In this invention, the combination of a rotating cap, a sealing cap, an annular sealing gasket, and a fixing nut forms a stable conductive structure that can be switched by rotation. This structure allows the reagent bottle body to connect with an external container. Combined with a commercially available syringe body, the built-in volume scale ensures the accuracy of pipetting, thereby achieving precise injection or aspiration of liquid and ultimately achieving closed pipetting of small volumes.

[0017] In this invention, by cooperating with the annular sealing gaskets in the first and third sealing ring grooves respectively, and installing sealing O-rings in each sealing ring groove, a tight seal can be achieved between the rotating cap and the sealing cap. After installing sealing O-rings in the second sealing ring groove, the sealing between the rotating cap and the syringe body can be guaranteed. Through the above-mentioned double sealing setup, the entire reactor is ensured to remain in a sealed state during the liquid transfer process, achieving both a closed liquid transfer effect and avoiding reagent waste, experimental contamination, and liquid transfer accuracy deviations caused by liquid leakage.

[0018] In this invention, the design of the partition area, the first positioning groove, and the second positioning groove allows the experimenter to quickly determine the current position of the needle hole by observing the corresponding position of the partition area and the first or second positioning groove. This ensures that the positioning method can make the needle hole accurately correspond to the first or second pipetting hole in different areas, thereby realizing the accurate aspiration and injection of various reagents in a sealed environment.

[0019] In summary, this rotary sealed pipette reactor allows researchers to efficiently and accurately aspirate and inject various liquids in a closed environment. Furthermore, this invention is compatible with both manual operation and automated equipment, effectively ensuring the stability and repeatability of experiments. Simultaneously, the reactor's rotating cap, sealing cap, annular sealing gasket, and perforated connector are compact and small, enabling low-cost mass production through injection molding and machining processes. All other components utilize commercially available products, significantly reducing overall costs and resulting in high economic benefits. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0021] Figure 2 This is a schematic diagram of the overall structure of this utility model in an exploded view.

[0022] Figure 3 This is an exploded view of a portion of the structure of this utility model.

[0023] Figure 4 This is an exploded view of a portion of the structure of this utility model from another perspective.

[0024] Figure 5 This is a partial structural cross-sectional schematic diagram of the present invention.

[0025] Figure 6 This is a three-dimensional structural diagram of the rotating cover of this utility model.

[0026] Figure 7 This is a three-dimensional structural diagram of the sealing cap of this utility model.

[0027] Figure 8 This is a schematic cross-sectional view of the sealing cap of this utility model.

[0028] Figure 9 This is a schematic diagram of the reagent bottle body structure of this utility model.

[0029] In the diagram: 1. Rotating cap; 11. Needle hole; 12. Annular groove; 13. First sealing ring groove; 14. Second sealing ring groove; 15. Through hole; 16. Third sealing ring groove; 17. Gear tooth; 18. Partition zone; 19. Vertical column; 2. Sealing cap; 21. First pipette orifice; 211. Anti-slip protrusion; 22. Second pipette orifice; 221. Second internal thread section; 23. Connector with hole; 231. Connector through hole; 232. Second external thread section; 233. Anti-slip textured section; 24. Center component; 241. First external thread section; 242. First internal thread section; 25. Center hole; 26. First positioning groove; 27. Second positioning groove; 28. Anti-slip groove; 29. ​​Third internal thread section; 3. Annular sealing gasket; 31. Sealing gasket through hole; 4. Nut washer; 5. Fixing nut; 6. Pin hole; 100. Syringe body; 110. Cylindrical barrel; 120. Needle; 130. Piston rod; 200. Reagent bottle body; 210. Bottle body; 220. Bottle cavity; 230. Third external thread section. Detailed Implementation

[0030] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0031] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0032] like Figures 1-9As shown, this utility model provides a rotary sealed pipetting reactor, including a rotary cap 1 for insertion into a syringe body 100 and a sealing cap 2 for detachable connection to a reagent bottle body 200. The end of the rotary cap 1 away from the syringe body 100 is fitted onto the end of the sealing cap 2 away from the reagent bottle body 200. An annular sealing gasket 3 is disposed in the upper cavity of the sealing cap 2 between the sealing cap 2 and the rotary cap 1. Multiple sealing gasket through holes 31 are formed through the surface of the annular sealing gasket 3. A needle hole 11 corresponding to the sealing gasket through hole 31 is formed through the rotary cap 1. At least one first pipetting hole 21 and at least one second pipetting hole 22 are formed through the sealing cap 2. One end of each of the first and second pipetting holes 21 is used to correspond to the sealing gasket through hole 31. The sum of the number of the first and second pipetting holes 21 and the number of the sealing gasket through hole 22 is equal to the number of the sealing gasket through hole 31. The number of through holes 31 is equal, and the other end of the first pipette hole 21 is located in the lower cavity of the sealing cap 2, and is used to draw liquid from the inner cavity of the reagent bottle body 200 or inject liquid into the inner cavity of the reagent bottle body 200 through the fluid connection fitting. The other end of the second pipette hole 22 is located on the circumferential wall of the sealing cap 2, and the other end of the second pipette hole 22 is provided with a perforated connector 23 for inserting external equipment or a connecting rigid tube or flexible tube of the reagent bottle body 200. Multiple perforated connectors 23 are provided, corresponding to the number of second pipette holes 22. The upper cavity of the sealing cap 2 and the central area of ​​the sealing cap 2 are integrally formed with a central member 24. A central hole 25 is opened through the central member 24 along the axial direction of the sealing cap 2. One end of the central member 24 penetrates the bottom of the rotating cap 1 and extends into the inner cavity of the rotating cap 1. The end of the central member 24 located in the inner cavity of the rotating cap 1 is detachably connected with a fastener.

[0033] Combination Figure 2 , Figure 3 , Figure 4 and Figure 9 As shown, both the syringe body 100 and the reagent bottle body 200 are commercially available components. The syringe body 100 includes a barrel 110, a needle tube 120 connected to the bottom of the barrel 110, and a piston rod 130 located inside the barrel 110. When the piston rod 130 is pulled or pushed, liquid can enter or exit the interior of the barrel 110 from the needle tube 120. The reagent bottle body 200 includes a bottle body 210, a bottle cavity 220, and a third external thread section 230. The outer wall of the sealing cap 2 is provided with an anti-slip groove 28 along the circumferential direction, and the inner wall of the lower cavity of the sealing cap 2 is provided with a third internal thread section 29. The sealing cap 2 is connected to the third external thread section 230 of the reagent bottle body 200 through the third internal thread section 29.

[0034] The syringe body 100 can be equipped with commercially available syringes of various sizes without needles, depending on the required pipetting volume. During operation, the operator can use automated equipment or manually pull the piston rod 130 to create positive or negative pressure to drive the liquid flow inside the reactor. The barrel 110 is used to connect with a matching rotating cap 1, and the outer wall of the barrel 110 and the inner wall of the rotating cap 1 are sealed together using commercially available O-rings. The outer diameter of the syringe needle 120 is compatible with the inner diameter of the needle hole 11, and they can be connected by an interference fit to ensure a tight seal at the connection.

[0035] It is worth noting that the bottle cavity 220 of the reagent bottle body 200 is divided into four regions by partitions, and each region corresponds to one of the first pipette holes 21. When one end of each of the four first pipette holes 21 enters the bottle cavity 220, the partitions inside the bottle cavity 220 are positioned low, so one end of the first pipette hole 21 will not touch the partition. Furthermore, when the reagent bottle body 200 is connected to the sealing cap 2, the initial position of one end of each of the four first pipette holes 21 can be close to one partition in each region. When the three internal thread section 29 and the three external thread section 230 are threaded together, one end of each of the four first pipette holes 21 can gradually move closer to another partition in each region. Thus, when the first pipette hole 21 is about to approach another partition, the three internal thread section 29 and the three external thread section 230 can complete the threaded connection, causing the sealing cap 2 to be threadedly connected to the reagent bottle body 200. It is worth noting that one end of each first pipette hole 21 moves at a certain angle in each corresponding region cavity, which can cause the third internal thread section 29 to connect with the third external thread section 230, without requiring the sealing cap 2 or the reagent bottle body 200 to make a 360-degree or higher circular motion.

[0036] When the reagent bottle body 200 is connected to the sealing cap 2, a flexible silicone tube can be fitted onto one end of each first pipette hole 21 on the sealing cap 2. The end of the silicone tube away from the first pipette hole 21 is used to guide the liquid into the inner cavity of the four areas, thereby allowing the liquid to be drawn or injected through the silicone tube. It should be noted that, due to the elasticity of the silicone tube, even if the sealing cap 2 or the reagent bottle body 200 is rotated excessively, one end of the silicone tube will always remain within the corresponding bottle cavity 220, without affecting operation; and the reagent bottle body 200 uses a commercially available transparent reagent bottle, making it convenient for laboratory personnel to observe the position of the first pipette hole 21 and the silicone tube through the transparent bottle body.

[0037] Combination Figure 3 and Figure 5As shown, as a further step, the fastener includes a nut washer 4 and a fixing nut 5. The nut washer 4 is used to fit onto one end of the center member 24, and the fixing nut 5 is used to thread onto one end of the center member 24. The nut washer 4 is used to fit onto the end of the center member 24 that extends into the inner cavity of the rotating cover 1, so as to ensure that the fixing nut 5 is not easy to loosen during the rotation of the rotating cover 1.

[0038] Combination Figure 7 and Figure 8 As shown, one end of the central component 24 has a pin hole 6 for placing a pin. By inserting the pin into the pin hole 6, it can be ensured that the fixing nut 5 will not come off the end of the central component 24, thereby preventing the rotating cover 1 from separating from the sealing cover 2 during rotation. It should also be noted that the pin can be a customized extremely small size, with its body end used to insert into the pin hole 6, and its head end used to limit the pin hole 6 from the outside, and the head end can have two parallel planes in the circumferential direction; thus, when the syringe body 100 is inserted into the inner cavity of the rotating cover 1, the pin will not affect the operation of the syringe body 100.

[0039] The outer wall of one end of the central component 24 is provided with a first external thread section 241 for connecting with the fixing nut 5, and the inner wall of one end of the central component 24 is provided with a first internal thread section 242. The first internal thread section 242 and the first external thread section 241 are located at the same end of the central component 24.

[0040] As one implementation method, when this solution adopts Figure 9 When the reagent bottle body 200 is shown, the first internal thread section 242 is used to install a commercially available filter element. The filter element is used to enable gas exchange between the reagent bottle body 200 and the external environment, while preventing external impurities from entering the bottle.

[0041] As another implementation, when the reagent bottle body 200 has no partition in the bottle cavity 220, the first internal thread section 242 can be used to install a commercially available thermometer, stirring rod or filter element. This allows the thermometer to monitor the reagent temperature in the reagent bottle body 200 without partition in real time, ensuring the stability of experimental conditions. The stirring rod is used to achieve uniform mixing of the reagent in the reagent bottle body 200 without partition, avoiding local concentration differences from affecting experimental accuracy.

[0042] It is worth noting that when the components are installed in the first internal thread section 242 in the above two embodiments, the operation is performed after the liquid is transferred in a closed manner, that is, it does not conflict with the operation of the syringe body 100 when drawing or injecting liquid.

[0043] As a further step, an anti-slip protrusion 211 is provided on the outer wall of one end of the first pipette 21, and a second internal thread section 221 is provided on the inner wall of one end of the second pipette 22. The first pipette 21 and the second pipette 22 are distributed at intervals along the circumferential direction on the sealing cap 2.

[0044] Combination Figure 7 and Figure 8 As shown, in this embodiment, four first pipette holes 21 and four second pipette holes 22 are provided respectively, and a second pipette hole 22 is provided between every two adjacent first pipette holes 21, and a first pipette hole 21 is provided between every two adjacent second pipette holes 22. The four first pipette holes 21 and the four second pipette holes 22 are arranged in a circumferential array and alternately spaced on the sealing cap 2.

[0045] Each of the four first pipette holes 21 has an anti-slip protrusion 211 on one end of its outer wall. When used according to actual needs, a silicone tube can be fitted onto one end of the outer wall of the first pipette hole 21 to allow for the aspiration or injection of liquid from different cavities 220 within the reagent bottle body 200. The main function of the anti-slip protrusion 211 is to increase friction and prevent the first pipette hole 21 from falling off when the silicone tube is fitted.

[0046] When a silicone tube is fitted onto the outer wall of one end of the first pipette hole 21, the corresponding reagent bottle body 200 is... Figure 9 The bottle shown in the image has a partition; when the inner cavity of the reagent bottle body 200 is integrated and no partition is used, a sample needle can be inserted into one end of the first pipetting hole 21 to make the sample needle contact the bottom of the bottle body cavity 220 for liquid aspiration or injection.

[0047] Combination Figure 3 and Figure 5 As shown, the inner wall of one end of each of the four second pipetting holes 22 is provided with a second internal thread section 221. The connector 23 with holes is provided with a connector through hole 231 along the axial direction. The outer wall of the connector 23 with holes is provided with a second external thread section 232 and an anti-slip texture section 233. The second external thread section 232 is used to be threadedly connected to the second internal thread section 221 on the inner wall of the second pipetting hole 22.

[0048] Specifically, the connector through hole 231 is used to insert the connecting hard tube or soft tube of external equipment or reagent bottle to realize the communication between external fluid and the second pipetting hole 22; while the threaded engagement of the second external thread section 232 and the second internal thread section 221 can enable the external container or reagent bottle to be tightened by manually turning the anti-slip textured section 233.

[0049] As a further step, the sealing gasket through holes 31 are provided with n, and n≥2. When n is even, the n sealing gasket through holes 31 are axially symmetrically distributed with reference to the central axis of the annular sealing gasket 3. When n is odd, the n sealing gasket through holes 31 are axially symmetrically distributed or asymmetrically distributed along the circumference.

[0050] Combination Figure 3 As shown, in this embodiment, there are eight sealing gasket through holes 31, which are arranged in a circumferential array. Four sealing gasket through holes 31 correspond to four first pipetting holes 21, and four sealing gasket through holes 31 correspond to four second pipetting holes 22.

[0051] Combination Figure 5 As shown, as a further step, the rotating cover 1 is provided with an annular groove 12 at one end near the sealing cover 2. The inner wall of the annular groove 12 is provided with at least one first sealing ring groove 13 for placing the sealing ring, and the inner cavity of the rotating cover 1 is provided with at least one second sealing ring groove 14 for placing the sealing ring. A through hole 15 is provided on one side of the needle hole 11 on the rotating cover 1. One end of the center member 24 is provided inside the through hole 15, and the inner wall of the through hole 15 is provided with at least one third sealing ring groove 16 for placing the sealing ring.

[0052] Specifically, to prevent liquid leakage, commercially available O-rings can be installed in the first sealing ring groove 13, the second sealing ring groove 14, and the third sealing ring groove 16, respectively, so that the specifications of the O-rings correspond to each sealing ring groove. Through the above arrangement, the first sealing ring groove 13 and the second sealing ring groove 14 can form an effective seal at the connection between the sealing cap 2 and the rotating cap 1, and the third sealing ring groove 16 can form an effective seal at the connection between the rotating cap 1 and the barrel 110 of the syringe body 100. This ensures that the entire reactor is always in a sealed state during the liquid transfer process, achieving the effect of closed liquid transfer, while avoiding reagent waste, experimental contamination, and liquid transfer accuracy deviation caused by liquid leakage.

[0053] Combination Figure 3 , Figure 4 and Figure 6 As shown, as a further step, the outer wall of the rotating cover 1 is provided with gear teeth 17 along the circumferential direction, and one end of one gear tooth 17 is provided with a partition area 18. The outer wall of the rotating cover 1 is provided with a vertical column 19 corresponding to the partition area 18. The central longitudinal section of the vertical column 19 along the length direction is on the same plane as the center of the needle hole 11.

[0054] Specifically, the rotating cap 1 is equipped with a vertical column 19 and a partition area 18, which facilitates visual observation of the position of the syringe 120 and the syringe orifice 11. This allows the syringe orifice 11 to be adjusted to the position corresponding to the first pipetting hole 21 or the second pipetting hole 22, thereby enabling the syringe body 100 to communicate with the corresponding first pipetting hole 21 or the second pipetting hole 22. This facilitates sealed pipetting of liquids in different reagent chambers. Furthermore, during the adjustment of the syringe orifice 11, when one syringe orifice 11 corresponds to one first pipetting hole 21 or the second pipetting hole 22, the other first pipetting holes 21 and the second pipetting holes 22 are not connected to the syringe orifice 11, thus achieving the effect of sealing the other pipetting holes.

[0055] The gear teeth 17 facilitate manual gripping or rotation of the rotating cap 1 by automated equipment, so that the needle tube 120, which is interference-fitted in the needle tube hole 11, can correspond to the first pipetting hole 21 or the second pipetting hole 22 through the sealing gasket through hole 31, enabling the needle tube 120 to communicate with each reagent area, and realize the accurate aspiration and injection of various reagents in a sealed environment.

[0056] It should be noted that when this utility model is used according to specific experimental needs, the number of the first pipette wells 21 or the second pipette wells 22 can be designed according to the required types and quantities of reagents to be added, such as two first pipette wells 21 or two second pipette wells 22, two first pipette wells 21 or three second pipette wells 22, three first pipette wells 21 or three second pipette wells 22, or three first pipette wells 21 or two second pipette wells 22. The number of sealing gasket through holes 31 is the sum of the number of first pipette wells 21 and the number of second pipette wells 22.

[0057] In practical use, the number of the first pipette wells 21 and the second pipette wells 22 can be flexibly adjusted according to the types and quantities of reagents required for the experiment. For example, when two reagents are needed, two first pipette wells 21 and zero second pipette wells 22, or zero first pipette wells 21 and two second pipette wells 22, can be set; when four reagents are needed, two first pipette wells 21 and two second pipette wells 22 can be set; when five reagents are needed, two first pipette wells 21 and three second pipette wells 22, or three first pipette wells 21 and two second pipette wells 22, etc. Correspondingly, the number of sealing gasket through-holes 31 is always equal to the sum of the number of first pipette wells 21 and the number of second pipette wells 22, to ensure that each pipette well can be sealed with a corresponding sealing gasket through-hole 31, meeting the needs of different experimental scenarios. It should also be noted that in this invention, the number of sealing gasket through holes 31 on the annular sealing gasket 3 needs to take into account practical factors such as processing accuracy. Under conventional sizes, the number can be set to a maximum of 32. However, when the annular sealing gasket 3 adopts a larger size design, the number of sealing gasket through holes 31 is not limited to 32 and can be set to more according to actual needs to adapt to the usage requirements of different scenarios. Correspondingly, the sum of the number of the first pipetting hole 21 and the second pipetting hole 22 is also adjusted synchronously with the number of sealing gasket through holes 31 to ensure that the correspondence is always established.

[0058] Furthermore, in a specific embodiment, this article takes a common scenario of mixing four reagents by pipetting as an example: In this case, the sealing cap 2 is provided with four first pipetting holes 21 and four second pipetting holes 22, which are alternately distributed along the circumference; correspondingly, the number of sealing gasket through holes 31 on the annular sealing gasket 3 is 8 (that is, the sum of the number of the four first pipetting holes 21 and the four second pipetting holes 22), and each sealing gasket through hole 31 is respectively set with one first pipetting hole 21 or one second pipetting hole 22 to meet the pipetting and sealing requirements of the four reagents.

[0059] As a further step, the sealing cap 2 is provided with at least one first positioning groove 26 and at least one second positioning groove 27 along the circumferential direction. The number of first positioning grooves 26 is equal to the number of first pipetting holes 21, and the number of second positioning grooves 27 is equal to the number of second pipetting holes 22. The first positioning grooves 26 are arranged in a one-to-one correspondence with the first pipetting holes 21, and the second positioning grooves 27 are arranged in a one-to-one correspondence with the second pipetting holes 22.

[0060] Combination Figure 7 As shown, in this embodiment, the sealing cap 2 is provided with four first positioning grooves 26 and four second positioning grooves 27 at equal intervals along the circumferential direction. The four first positioning grooves 26 correspond one-to-one with the four first pipetting holes 21, and the four second positioning grooves 27 correspond one-to-one with the four second pipetting holes 22.

[0061] Specifically, through the design of four first positioning grooves 26 and four second positioning grooves 27, when the isolation zone 18 corresponds to the first positioning groove 26, the needle hole 11 can correspond to the first pipetting hole 21; and when the isolation zone 18 corresponds to the second positioning groove 27, the needle hole 11 can correspond to the second pipetting hole 22, which facilitates precise manual adjustment to the position of the reagent bottle to be aspirated or injected for precise liquid aspiration and injection.

[0062] Working principle and usage process of this utility model: During assembly, first place the annular sealing gasket 3 in the upper cavity of the sealing cover 2, so that the sealing gasket through hole 31 corresponds to the first pipetting hole 21 and the second pipetting hole 22 respectively. Then, put the rotating cover 1 on the sealing cover 2 and put the nut washer 4 on one end of the center piece 24. Then, the fixing nut 5 is threadedly connected to one end of the center piece 24 located in the inner cavity of the rotating cover 1, so that the rotating cover 1 and the sealing cover 2 are firmly connected. Insert the connecting rigid tube or flexible tube of the external device or reagent bottle into the connector through hole 231 respectively, and then allow the external fluid to communicate with the second pipette hole 22 through the cooperation of the second external thread section 232 and the second internal thread section 221; then thread the sealing cap 2 onto the commercially available reagent bottle body 200, so that one end of each first pipette hole 21 corresponds to one of the inner cavity areas on the reagent bottle body 200; Insert the pin into the pin hole 6 to restrict the displacement of the fixing nut 5, and then insert the commercially available syringe body 100 into the inner cavity of the rotating cover 1 so that the syringe needle 120 is interference-fitted into the needle hole 11. During this process, one end of the syringe barrel 110 can abut against one end of the center member 24.

[0063] During assembly, commercially available O-rings can be placed in the first sealing ring groove 13, the second sealing ring groove 14, and the third sealing ring groove 16, respectively. One end of each of the multiple first pipette holes 21 can be fitted with a syringe needle or a silicone tube, depending on the state of the reagent bottle body 200. This article uses an example where the reagent bottle body 200 has a partition and is fitted with a silicone tube.

[0064] In use, the experimenter can use automated equipment or manually pull the piston rod 130 to create positive and negative pressure to drive the liquid flow inside the reactor. For example, when the needle hole 11 corresponds to a first pipetting hole 21 through the sealing gasket through hole 31, the syringe body 100 can accurately draw and inject liquid into an inner cavity area on the reagent bottle body 200, and the liquid flows through the silicone tube; when the needle hole 11 corresponds to a second pipetting hole 22 through the sealing gasket through hole 31, the syringe body 100 can accurately draw and inject liquid into external equipment or reagent bottles to achieve small-volume closed pipetting.

[0065] During use, the experimenter can manually hold or drive the gear 17 on the rotating cover 1 with the help of automated equipment to rotate the rotating cover 1. During the rotation of the rotating cover 1, the experimenter can observe the corresponding position of the first positioning groove 26 and the second positioning groove 27 through the partition area 18, so that the needle hole 11 can correspond to the first pipetting hole 21 or the second pipetting hole 22 in different areas, thereby realizing the accurate aspiration and injection of various reagents in a sealed environment.

[0066] In summary, this rotary sealed pipette reactor allows researchers to efficiently and accurately aspirate and inject various liquids in a closed environment. Furthermore, this invention is compatible with both manual operation and automated equipment, effectively ensuring the stability and repeatability of experiments. Simultaneously, the rotating cap 1, sealing cap 2, annular sealing gasket 3, and perforated connector 23 are compact and small in structure, enabling low-cost mass production through injection molding and machining processes. All other components utilize commercially available products, significantly reducing overall costs and resulting in high economic benefits.

[0067] In this invention, the term "plural" refers to two or more items unless otherwise expressly defined. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items. The terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; "linking" can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0068] It should be noted that when a component is referred to as being "assembled on," "mounted on," "fixed to," or "set on" another component, it can be directly on the other component or there may be an intermediate component. When a component is considered to be "connected to" another component, it can be directly connected to the other component or there may be an intermediate component present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0069] In the description of this specification, the terms "one embodiment," "some embodiments," "specific embodiment," etc., refer to a specific feature, structure, material, or characteristic described in connection with that embodiment or example, which is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0070] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A rotary sealed liquid transfer reactor, characterized in that, The device includes a rotating cap (1) for insertion into a syringe body (100) and a sealing cap (2) for detachable connection to a reagent bottle body (200). The end of the rotating cap (1) away from the syringe body (100) is fitted onto the end of the sealing cap (2) away from the reagent bottle body (200). An annular sealing gasket (3) is provided in the upper cavity of the sealing cap (2) between the sealing cap (2) and the rotating cap (1). A plurality of sealing gasket through holes (31) are provided through the surface of the annular sealing gasket (3). A needle hole (11) corresponding to the sealing gasket through hole (31) is provided through the rotating cap (1). At least one first pipette hole (21) and at least one second pipette hole (22) are provided through the sealing cap (2). One end of the first pipette hole (21) and the second pipette hole (22) are both The first pipette hole (21) and the second pipette hole (22) are configured to correspond to the sealing gasket through hole (31), and the sum of the number of the first pipette hole (21) and the second pipette hole (22) is equal to the number of the sealing gasket through hole (31). The other end of the first pipette hole (21) is located in the lower cavity of the sealing cover (2), and the other end of the second pipette hole (22) is located on the circumferential wall of the sealing cover (2). The other end of the second pipette hole (22) is provided with a perforated connector (23). The central area of ​​the sealing cover (2) is integrally formed with a central component (24). A central hole (25) is provided through the central component (24) along the axial direction of the sealing cover (2). One end of the central component (24) penetrates the bottom of the rotating cover (1) and extends to the inner cavity of the rotating cover (1). The end of the central component (24) located in the inner cavity of the rotating cover (1) is detachably connected with a fastener.

2. The rotary sealed liquid transfer reactor according to claim 1, characterized in that, The fastener includes a nut washer (4) and a fixing nut (5). The nut washer (4) is used to be sleeved on one end of the center piece (24), and the fixing nut (5) is used to be threaded to one end of the center piece (24). The outer wall of one end of the center piece (24) is provided with a first external thread section (241) for connecting with the fixing nut (5), and the inner wall of one end of the center piece (24) is provided with a first internal thread section (242). The first internal thread section (242) and the first external thread section (241) are located at the same end of the center piece (24).

3. The rotary sealed liquid transfer reactor according to claim 1, characterized in that, The outer wall of one end of the first pipette (21) is provided with an anti-slip protrusion (211), and the inner wall of one end of the second pipette (22) is provided with a second internal thread section (221). The first pipette (21) and the second pipette (22) are distributed at intervals along the circumferential direction on the sealing cap (2).

4. The rotary sealed liquid transfer reactor according to claim 1, characterized in that, The sealing gasket through holes (31) are provided in n, and n≥2. When n is even, the n sealing gasket through holes (31) are axially symmetrically distributed with respect to the central axis of the annular sealing gasket (3). When n is odd, the n sealing gasket through holes (31) are axially symmetrically distributed or asymmetrically distributed along the circumference.

5. A rotary sealed liquid transfer reactor according to claim 1, characterized in that, The rotating cover (1) has an annular groove (12) at one end near the sealing cover (2). The inner wall of the annular groove (12) has at least one first sealing ring groove (13) for placing the sealing ring, and the inner cavity of the rotating cover (1) has at least one second sealing ring groove (14) for placing the sealing ring.

6. The rotary sealed liquid transfer reactor according to claim 1, characterized in that, The needle hole (11) has a through hole (15) on one side of the rotating cover (1), and one end of the center piece (24) is used to be set inside the through hole (15). The inner wall of the through hole (15) has at least one third sealing ring groove (16) for placing the sealing ring.

7. A rotary sealed liquid transfer reactor according to claim 1, characterized in that, The outer wall of the rotating cover (1) is provided with gear teeth (17) along the circumferential direction, and one end of one gear tooth (17) is provided with a partition area (18). The outer wall of the rotating cover (1) is provided with a vertical column (19) corresponding to the partition area (18). The central longitudinal section of the vertical column (19) along the length direction is on the same plane as the center of the needle hole (11).

8. A rotary sealed liquid transfer reactor according to claim 1, characterized in that, The sealing cap (2) is provided with at least one first positioning groove (26) and at least one second positioning groove (27) along the circumferential direction. The number of the first positioning grooves (26) is equal to the number of the first pipette holes (21), and the number of the second positioning grooves (27) is equal to the number of the second pipette holes (22). The first positioning grooves (26) are arranged in a one-to-one correspondence with the first pipette holes (21), and the second positioning grooves (27) are arranged in a one-to-one correspondence with the second pipette holes (22).

9. A rotary sealed liquid transfer reactor according to claim 3, characterized in that, The perforated connector (23) has a through hole (231) extending along the axial direction, and the outer wall of the perforated connector (23) is provided with a second external thread section (232) and an anti-slip texture section (233). The second external thread section (232) is used to thread-connect with the second internal thread section (221) on the inner wall of the second pipetting hole (22).

10. A rotary sealed liquid transfer reactor according to claim 1, characterized in that, The outer wall of the sealing cap (2) is provided with an anti-slip groove (28) along the circumferential direction, and the inner wall of the lower cavity of the sealing cap (2) is provided with a third internal thread section (29). The sealing cap (2) is connected to the reagent bottle body (200) through the third internal thread section (29).

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