Composition with a cell culture extract from Bacillus subtilis and an additional active ingredient for the care of hair and scalp.

Abstract

The invention relates to a cosmetic composition for the care of hair and scalp, comprising a first active ingredient selected from the group consisting of bacitracin, amyloslysine, plantazolicin, bacillomycin D, lactosporin, thuricin CD, bacillaene, and mixtures thereof; and a cell culture extract from Bacillus subtilis. The invention further relates to the use of this composition for the care of hair and scalp and for preventing dandruff on the scalp.

Description

field of technology

[0001] The invention relates to cosmetic compositions for the care of hair and scalp, in particular for cleaning the hair and for combating dandruff on the scalp. Current state of the art

[0002] Even slight flaking of the scalp or other hairy areas of the body is perceived as a sign of poor care and hygiene. Dandruff is also usually accompanied by an intense itching, which can trigger scratching and lead to injuries of the affected skin. Skin injuries, in turn, often provide a breeding ground for infections and pathogens.

[0003] Dandruff can be caused by various factors such as hereditary predisposition, a tendency towards increased sebum production (seborrhea), hormonal fluctuations, stress, climatic conditions, or improper hair care. However, the decisive factor in dandruff formation is the colonization of the scalp with yeast fungi of the genus Malassezia.

[0004] Therefore, the goal in combating dandruff has always been to reduce and / or eliminate the colonization of the scalp by Malassezia bacteria. Effective anti-dandruff agents are known for this purpose, such as piroctone olamines, climbazole, zinc pyrithione, sulfur, and / or selenium sulfide. The effect of these anti-dandruff agents is primarily based on their fungicidal properties.

[0005] However, the aforementioned anti-dandruff agents have disadvantages. For example, in addition to a certain potential for skin irritation, they have low water solubility and are therefore difficult to incorporate into conventional cosmetic products. Another disadvantage is their low biodegradability, which is why their use in cosmetic products is increasingly being strictly regulated or even prohibited.

[0006] Natural-based anti-dandruff agents cannot completely replace the aforementioned anti-dandruff agents, as they often do not show sufficient, immediate and / or long-lasting effectiveness.

[0007] Therefore, there remains a need for compounds with good efficacy against dandruff that are accessible from natural sources and biodegradable, and that can be easily and readily incorporated into cosmetic cleansing compositions. Technical task

[0008] The present invention aims to provide cosmetic compositions for the care of hair and scalp that help to reduce dandruff, strengthen the natural skin barrier, and moisturize the hair and scalp. Furthermore, the compositions are intended to be suitable for the manufacture of conventional hair cosmetic compositions, in particular shampoos. Description of the invention

[0009] A first object of the invention is a cosmetic composition for the care of hair and scalp comprising a first active ingredient selected from the group consisting of bacitracin, amyloslysine, plantazolicin, bacillomycin D, lactosporin, thuricin CD, bacillaene and mixtures thereof; and a cell culture extract from Bacillus subtilis.

[0010] Preferably, the cell culture extract is obtained from the cell-free supernatant of a cell culture fluid.

[0011] Preferably, the cell culture extract is obtained from Bacillus subtilis strains whose 16S ribosomal RNA sequence has at least 97.0% sequence identity to one of the RNA sequences selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4.

[0012] Preferably, the cell culture extract includes the active ingredient bacilysin.

[0013] Preferably, the composition contains 0.1 wt.% to 5 wt.% of the cell culture extract.

[0014] Preferably, the composition contains 0.01 wt.% to 5 wt.% of the first active ingredient.

[0015] Preferably, the composition comprises a surfactant system comprising at least one anionic and at least one amphoteric surfactant.

[0016] Preferably, the composition comprises at least one cationic polymer and / or at least one oil.

[0017] A second object of the invention is the use of a composition according to the invention for the care of hair and scalp.

[0018] Its use is primarily intended to prevent dandruff on the scalp. Advantageous effects of the invention

[0019] The cosmetic composition according to the invention has excellent conditioning properties for hair and scalp. In particular, the composition according to the invention can prevent dandruff on the scalp. Surprisingly, it has been shown that the use of a cell culture extract from Bacillus subtilis significantly contributes to these effects. Presumably, the cell culture extract according to the invention inhibits the growth of microorganisms involved in the development of flaky scalp, for example, microorganisms of the species Malassezia. The composition can therefore also counteract redness and irritation of the scalp. The composition according to the invention helps to reduce dandruff, strengthen the natural skin barrier, and moisturize hair and scalp.

[0020] Furthermore, it has surprisingly been shown that the effect of this cell culture extract is enhanced by the addition of a specific active ingredient. The combination of cell culture extract and additional active ingredient thus constitutes an effective anti-dandruff agent.

[0021] Furthermore, it has been shown that the combination of cell culture extract and additional active ingredient according to the invention is ideally suited for the production of shampoos. Description of the embodiments

[0022] The following section describes in detail embodiments of the present invention. However, the present invention is not limited to the following embodiments.

[0023] A first object of the invention is a cosmetic composition for the care of hair and scalp. The composition according to the invention can, in particular, be a hair cosmetic composition. For example, the composition is a shampoo, a conditioner, or a hair lotion. Preferably, it is a shampoo.

[0024] A second aspect of the invention is the cosmetic use of this composition for the care of hair and scalp. The composition can be used, for example, for the care, in particular for cleansing or conditioning, of hair. The composition can also be used for cleansing the scalp. Preferably, the composition is additionally used to prevent dandruff on the scalp. Cell culture extract

[0025] A key component of the composition according to the invention is a cell culture extract from Bacillus subtilis. The cell culture extract is obtained by cultivating microorganisms in a nutrient solution (fermentation). According to the invention, microorganisms of the species Bacillus subtilis are used for this purpose. Surprisingly, it has been shown that a cell culture extract obtained from these microorganisms exhibits good conditioning properties for hair and scalp and is particularly well suited as an anti-dandruff agent.

[0026] Within the scope of this invention, a cell culture extract is defined as a composition obtained from a cell culture fluid, wherein the cell culture fluid comprises microorganisms in a nutrient solution. The cell culture extract can, in particular, represent a cell-free fraction of the cell culture fluid.

[0027] Preferably, the cell culture extract is a cell-free supernatant from a cell culture fluid. The cell-free supernatant can be obtained, for example, by centrifugation and / or filtration of a cell culture fluid. In a particularly preferred embodiment, the cell-free supernatant is obtained by pelletizing the cells from a cell culture fluid by centrifugation at 4000 g to 8000 g and passing the supernatant through a 0.2 µM filter. A supernatant obtained in this way is essentially free of viable cells and contains, at most, traces of cells or cell debris.

[0028] In another embodiment, the cell culture extract can be obtained from the cell pellet of a cell culture fluid. Cell pellets can be obtained by centrifuging the cell culture fluid and removing the cell-free supernatant. In an exemplary embodiment, the pellet is resuspended in acidic water at a pH of 2–4, re-pelleted by centrifugation at preferably 4000 x g to 8000 x g, and the supernatant is passed through a 0.2 µM filter. Optionally, the cell pellet can be resuspended in alkaline water with a pH above 8.5. Producing the cell culture extract from a cell pellet has the advantage that the resuspension yields a cell suspension with a higher concentration than that of the cell culture fluid. In this way, a more concentrated suspension can be produced to generate an effective amount of the active cell culture extract.Furthermore, resuspending the pellet in water or in liquids that are not a nutrient solution leads to a reduction or elimination of the inactive components of the nutrient solution that may interfere with the active ingredients of the cell culture extract.

[0029] In a preferred embodiment, the cell culture extract contains no viable cells of microorganisms. In a preferred embodiment, the microorganisms used to produce the cell culture extract are subjected to a treatment that renders them incapable of reproduction, e.g., by heat, dehydration, gamma radiation, or UV irradiation. This is carried out after the microorganisms have multiplied in the nutrient solution. Preferably, this treatment is performed during the production of the cell culture extract. For example, the cell-free supernatant described above or the cell suspension obtained from a cell pellet can be subjected to such treatment to inactivate any remaining viable cells in the supernatant.

[0030] The nutrient medium used for the production of the cell culture extract is any medium containing the necessary nutrients suitable for the propagation of the microorganism(s) used in the present invention. Suitable nutrients include, but are not limited to, amino peptides, peptides, yeast extract, salts, sugars, carbohydrates, and / or vitamins. The medium can be prepared from dairy products such as milk, cereals, fruits, and / or vegetables.

[0031] The cell culture extract can be in solid or liquid form. For example, it can be a powder, granules, a suspension, or a solution. A solid cell culture extract can be produced, for example, by spray drying. In one embodiment, the cell culture extract is in the form of layered granules. The layered granules can comprise a core surrounded by a coating layer containing the cell culture extract. The core is preferably water-soluble. The cell culture extract is preferably embedded in a protective matrix, which preferably contains at least one polyhydroxy compound and at least one phosphate compound.

[0032] The following variants of Bacillus subtilis prove to be particularly advantageous for obtaining the cell culture extract, because a cell culture extract obtained from these microorganisms has particularly good conditioning properties for hair and scalp and is especially suitable as an anti-dandruff agent.

[0033] Preferably, the cell culture extract is obtained from Bacillus subtilis strains whose 16S ribosomal RNA sequence has at least 97.0%, preferably at least 98.0%, particularly preferably at least 99%, further preferably at least 99.5%, further preferably at least 99.8%, most preferably 100% sequence identity to one of the RNA sequences selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4.

[0034] SEQ ID NO: 1 is the 16S ribosomal RNA sequence of Bacillus subtilis MDI_063_G01 (referred to here as Bacillus subtilis G01), deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149857.

[0035] SEQ ID NO: 2 is the 16S ribosomal RNA sequence of Bacillus subtilis PC01, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149969.

[0036] SEQ ID NO: 3 is the 16S ribosomal RNA sequence of Bacillus subtilis N01, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149971.

[0037] SEQ ID NO: 4 is the 16S ribosomal RNA sequence of Bacillus subtilis K01, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149972.

[0038] The term "sequence identity," as used here in relation to a reference sequence, is defined as the percentage of nucleotide residues in a candidate sequence that are identical to the residues in the reference polynucleotide sequence after the sequences have been optimally aligned and gaps introduced, if necessary, to achieve maximum sequence identity. The alignment of the sequences to be compared is preferably calculated by pairwise sequence alignment using the Needleman-Wunsch algorithm.

[0039] In a preferred embodiment, the cell culture extract is obtained from Bacillus subtilis strains selected from the group consisting of the strains Bacillus subtilis MDI_063_G01 (here referred to as Bacillus subtilis G01), deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149857, Bacillus subtilis PC01, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149969, Bacillus subtilis N01, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149971, Bacillus subtilis K01, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS149972 and B. subtilis G01_spo, deposited at the Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS151098, or mixtures thereof.

[0040] In a preferred embodiment, the microorganisms are selected from Bacillus subtilis strains in which a SpoIIE sporulation gene has been knocked out.

[0041] The cell culture extract may contain, in particular, metabolites, such as unused nutrients and metabolic products of the microorganisms released into the nutrient solution. It may also contain, in particular, cell components of the microorganisms, such as bacterial peptides, polysaccharides, and lipids.

[0042] In a preferred embodiment, the cell culture extract comprises the active ingredient bacilysin. Bacilysin is a dipeptide produced by certain Bacillus species. Cell culture extracts containing bacilysin have been shown to have particularly good conditioning properties for hair and scalp, and in particular to exhibit good anti-dandruff effects. Cell culture extracts containing bacilysin can also be particularly effective in reducing the growth of microorganisms of the species Malassezia.

[0043] The composition comprises the cell culture extract preferably in an amount of 0.001 wt.% to 5 wt.%, preferably 0.01 wt.% to 2 wt.%, more preferably 0.1 wt.% to 1 wt.%, most preferably 0.2 wt.% to 0.6 wt.%, based on the total mass of the composition.

[0044] Preferably, the cell culture extract contains bacilysin in an amount of 0.01 wt.% to 25 wt.%, preferably 0.05 wt.% to 20 wt.%, particularly preferably 0.1 wt.% to 15 wt.%, based on the total weight of the cell culture extract.

[0045] In a further preferred embodiment, the cell culture extract is used in an amount such that the weight fraction of bacilysin in the composition according to the invention is 0.0001 wt.% to 1.25 wt.%, preferably 0.001 wt.% to 1 wt.%, and particularly preferably 0.005 wt.% to 0.75 wt.%, based on the total weight of the composition. Preferably, no more than 5 wt.% of the cell culture extract is used, based on the total weight of the composition. Additional active ingredient

[0046] A second essential component of the composition according to the invention is an additional active ingredient (the first active ingredient). The additional active ingredient is bacitracin, amyloslysine, plantazolicin, bacillomycin D, lactosporin, thuricin CD, bacillaene, or mixtures thereof. It has been shown that the combination of one of these active ingredients with the cell culture extract described above is particularly well suited for combating dandruff.

[0047] The aforementioned active ingredients can be obtained from Bacillus cultures. It is also possible to use cell culture extracts from Bacillus containing the respective active ingredient or a mixture of active ingredients instead of the pure substances. In one embodiment, the active ingredient is contained in the cell culture extract described above.

[0048] Bacitracin can be obtained in particular from Bacillus subtilis. Bacitracin has the following structure:

[0049] Amyloslysin can be obtained particularly from Bacillus amyloliquefaciens GA1.

[0050] Plantazolicin can be obtained in particular from Bacillus velezensis FZB42. Plantazolicin has the following structural formula:

[0051] Bacillomycin D can be obtained primarily from Bacillus subtilis. Bacillomycin D has the following structural formula:

[0052] Lactosporin can be obtained in particular from Bacillus coagulans ATCC 7050.

[0053] Thuricin CD can be obtained in particular from Bacillus thuringiensis DPC 6431.

[0054] The active ingredient is preferably selected from the group consisting of bacitracin, bacillomycin D, and bacillaene. These active ingredients can be obtained, in particular, from Bacillus subtilis and can therefore also be contained in the cell culture extract described above. Preferably, the active ingredient is bacitracin, bacillomycin D, or a mixture thereof.

[0055] The composition preferably contains the active ingredient in an amount of 0.001 wt.% to 5 wt.%, more preferably 0.01 wt.% to 5 wt.%, further preferably 0.01 wt.% to 1 wt.%, based on the total weight of the composition. surfactant system

[0056] In one embodiment, the composition is formulated as a liquid shampoo or as a solid hair soap and contains at least one surfactant for this purpose.

[0057] A solid composition preferably comprises surfactants in an amount of 10 wt.% to 60 wt.%, preferably 20 wt.% to 50 wt.%, most preferably 30 wt.% to 40 wt.%, based on the total weight of the composition.

[0058] A liquid composition preferably comprises surfactants in an amount of 1 wt.% to 40 wt.%, preferably 2 wt.% to 30 wt.%, most preferably 5 wt.% to 20 wt.%, based on the total weight of the composition.

[0059] Preferably, the surfactant is a surfactant system comprising at least one anionic surfactant and at least one amphoteric surfactant. Preferably, this surfactant system comprises 50 wt.% or more anionic surfactants, based on the total weight of surfactants.

[0060] Preferred anionic surfactants are alkyl sulfates, alkyl ether sulfates, sulfosuccinates, alkyl sulfonic acid esters, N-acylisethionates, N-methyl-N-acylisethionates, sulfoacetates, N-acylglycinates, N-acylsarcosinates, N-acylglutamates, N-acylalanines, fatty acid salts, N-acyltaurates, N-methyl-N-acyltaurates, phosphate esters, rhamnolipids, sophorlipids, or mixtures thereof. Anionic surfactants are preferably used as sodium, potassium, or ammonium salts.

[0061] Alkyl sulfates and alkyl ether sulfates are particularly preferred. Suitable alkyl sulfates and alkyl ether sulfates preferably have the general structural formula RO-[(CH2CH2)-O] n -SO3 -, where R represents a linear or branched alkyl group with 8 to 24 carbon atoms, and n is a number between 0 and 12. The number n indicates the average degree of ethoxylation of the alkyl ether sulfates. For pure alkyl sulfates, n = 0. Anionic surfactants with the INCI names Sodium Coco Sulfate and Sodium Laureth Sulfate (EO 1-4) are particularly preferred.

[0062] In an alternative embodiment, the surfactant system contains no or only a small amount of sulfate-containing surfactants, such as alkyl sulfates or alkyl ether sulfates. Preferably, the proportion of sulfate-containing surfactants in the surfactant system is 1 wt.% or less, more preferably 0.5 wt.% or less, further preferably 0.1 wt.% or less, and most preferably 0.01 wt.% or less, based on the total weight of all surfactants in the composition.

[0063] In a preferred embodiment, the surfactant system comprises an anionic surfactant from the group consisting of N-acyltaurates, N-methyl-N-acyltaurates, N-acylisethionates, N-methyl-N-acylisethionates, (alkyl)sulfonic acid esters and mixtures thereof.

[0064] A solid composition comprises anionic surfactants preferably in an amount of 10 wt.% to 60 wt.%, preferably 20 wt.% to 50 wt.%, most preferably 30 wt.% to 40 wt.%, based on the total weight of the composition.

[0065] A liquid composition comprises anionic surfactants preferably in an amount of 1 wt.% to 40 wt.%, preferably 2 wt.% to 30 wt.%, most preferably 5 wt.% to 20 wt.%, based on the total weight of the composition.

[0066] Preferred amphoteric surfactants are betaines, sultaines, hydroxysultaines, alkylampho(di)acetates and mixtures thereof.

[0067] Suitable betaines include, for example, N-alkyl-N,N-dimethylammonium glycinates, such as cocoalkyl-dimethylammonium glycinate; N-acyl-aminopropyl-N,N-dimethylammonium glycinate, such as cocoacylaminopropyldimethylammonium glycinate; and 2-alkyl-3-carboxymethyl-3-hydroxyethyl-imidazolines, each with 8 to 18 carbon atoms in the alkyl or acyl group, as well as cocoacylaminoethylhydroxyethyl-carboxymethyl glycinate. Particularly suitable betaines are the compounds with the INCI names cocamidopropyl betaine and lauramidopropyl betaine.

[0068] Particularly suitable alkylampho(di)acetates are the compounds with the INCI names Cocoampho(di)acetate and Lauroapho(di)acetate.

[0069] A solid composition comprises amphoteric surfactants preferably in an amount of 1 wt.% to 30 wt.%, preferably 2 wt.% to 20 wt.%, most preferably 3 wt.% to 15 wt.%, based on the total weight of the composition.

[0070] A liquid composition comprises amphoteric surfactants preferably in an amount of 0.1 wt.% to 20 wt.%, preferably 0.5 wt.% to 15 wt.%, most preferably 1 wt.% to 10 wt.%, based on the total weight of the composition.

[0071] In a preferred embodiment, the surfactant system comprises at least one alkyl sulfate or alkyl ether sulfate and at least one betaine.

[0072] In a further preferred embodiment, the surfactant system comprises at least one taurate, isethionate or alkyl sulfonic acid ester and at least one betaine.

[0073] The surfactant system may also preferably contain at least one non-ionic surfactant.

[0074] Suitable non-ionic surfactants can be selected from amine oxides, such as the compounds known under the INCI names Cocamine Oxide, Lauramine Oxide and Cocamidopropylamine Oxide, fatty acid alkanolamides, such as the compounds known under the INCI names Cocamide MEA and Coamide DEA, mixtures of alkyl (oligo)glucosides and fatty alcohols, such as the commercially available product Montanov. ® 68, sterols such as zoosterols and phytosterols, phospholipids, alkyl(oligo)glycosides, such as the compounds known under the INCI names caprylyl / capryl glucoside, decyl glucoside, lauryl glucoside and coco glucoside, alkoxylated esters, such as the compounds known under the INCI names PEG-7 glyceryl cocoate and PEG-40 hydrogenated castor, polyglycerol esters, and mannosylerythritol lipids (MEL).

[0075] Alkyl(oligo)glycosides are particularly preferred due to their foaming properties. Alkoxylated esters, especially PEG-7 glyceryl cocoate, are also preferred, as they additionally act as emollients and support or enable the solubilization of water-insoluble components, which can optionally be added to the formulations as conditioning agents.

[0076] A solid composition comprises non-ionic surfactants preferably in an amount of 1 wt.% to 30 wt.%, preferably 2 wt.% to 20 wt.%, most preferably 3 wt.% to 15 wt.%, based on the total weight of the composition.

[0077] A liquid composition comprises non-ionic surfactants preferably in an amount of 0.1 wt.% to 20 wt.%, preferably 0.5 wt.% to 15 wt.%, most preferably 1 wt.% to 10 wt.%, based on the total weight of the composition. solvent

[0078] The composition may also contain at least one solvent. Preferred solvents are water and alcohols. Suitable alcohols include, for example, ethanol, ethanediol, 1-propanol, 2-propanol, isopropanol, 1,2-propylene glycol, glycerin, diglycerin, triglycerin, 1-butanol, 2-butanol, 1,2-butanediol, 1,3-butanediol, 1-pentanol, 2-pentanol, 1,2-pentanediol, 1,5-pentanediol, 1,6-hexanediol, polyethylene glycols, sorbitol, sorbitan, benzyl alcohol, phenoxyethanol, or mixtures thereof.

[0079] The composition preferably comprises solvent in an amount of 50% by weight or more, more preferably 70% by weight or more, and most preferably 80% by weight or more, based on the total weight of the composition. The proportion of alcohol in the composition is preferably 30% by weight or less, more preferably 20% by weight or less, and most preferably 10% by weight or less, based on the total weight of the composition. Cationic polymers

[0080] In one embodiment, the composition comprises at least one cationic polymer. These have a hair-conditioning effect.

[0081] Suitable cationic polymers include, for example, quaternized cellulose polymers, such as polyquaternium-10, known as Celquat. ® and Polymer JR ® commercially available; hydrophobically modified cationic cellulose derivatives, such as polyquaternium-67; cationic alkyl polyglycosides; cationized honey, for example the commercial product Honeyquat ® 50; cationic galactomannan derivatives, in particular cationic guar and / or cassia polymers, such as those sold under the trade names Jaguar ® , N-Hance ® , Polycare ® , Clearance ®available products; cationic starch derivatives; cationic inulin polymers; polymeric dimethyldiallylammonium salts and their copolymers with esters and amides of acrylic acid and methacrylic acid, especially polyquaternium-6 and polyquaternium-7; copolymers of vinylpyrrolidone with quaternized derivatives of dialkylaminoalkyl acrylate and methacrylate, such as vinylpyrrolidone-dimethylaminoethyl methacrylate copolymers quaternized with diethyl sulfate; vinylpyrrolidone-vinylimidazolium methochloride copolymers, such as those marketed under the names Luviquat ® FC 370, FC 550, FC 905 and HM 552 are offered; quaternized polyvinyl alcohol; as well as the polymers known as Polyquaternium 2, Polyquaternium-4, Polyquaternium 17, Polyquaternium 18, Polyquaternium-24, Polyquaternium 27, Polyquaternium-32, Polyquaternium-37, Polyquaternium-39, Polyquaternium-74 and Polyquaternium 89.

[0082] Cationic polymers of natural origin such as quaternized cellulose polymers, hydrophobically modified cationic cellulose derivatives and / or cationic galactomannan polymers are preferred.

[0083] Particularly preferred are the cationic polymers known under the INCI names Guar Hydroxypropyltrimonium Chloride, Hydroxypropyl Guar Hydroxypropyltrimonium Chloride, Cassia Hydroxypropyltrimonium Chloride, Hydroxypropyltrimonium Inulin, Polyquaternium-10, Polyquaternium-67, or mixtures thereof.

[0084] The composition comprises cationic polymers preferably in an amount of 0.01 wt.% to 5 wt.%, preferably 0.02 wt.% to 3 wt.%, most preferably 0.05 wt.% to 2 wt.%, based on the total weight of the composition. oil

[0085] In one embodiment, the composition comprises at least one oil. This oil has a hair-conditioning effect.

[0086] Suitable oils include, in particular, mineral oils, dialkyl ethers, fatty alcohols, dicarboxylic acid esters, fatty acid glycerol esters, ester oils, waxes and vegetable oils.

[0087] Ester oils are esters of fatty acids and aliphatic or aromatic alcohols. Suitable ester oils include, for example, isopropyl myristate, cetyl ethylhexanoate, cetearyl ethylhexanoate, and octyldodecyl stearate.

[0088] Suitable vegetable oils include argan oil, coconut oil, jojoba oil, olive oil, grapeseed oil, almond oil, avocado oil, castor oil, macadamia oil, and hemp oil. Hydrogenated and, if necessary, alkoxylated vegetable oils are preferred, such as hydrogenated and ethoxylated castor oil.

[0089] Suitable waxes include, for example, carnauba wax, candelilla wax, fruit waxes, beeswax, wool wax, spermaceti, preen gland sebum, hard paraffin, ceresin, ozokerite, ester waxes such as polyethylene glycol or polyethylene glycol ester waxes and / or hydrogenated vegetable oils.

[0090] The composition preferably comprises oils in an amount of 0.01 wt.% to 5 wt.%, more preferably 0.02 wt.% to 3 wt.%, most preferably 0.05 wt.% to 2 wt.%, based on the total weight of the composition. Auxiliary materials

[0091] The composition may include further cosmetic excipients. Preferably, the composition comprises at least one compound selected from the group consisting of additional anti-dandruff agents, polyalkylene glycol ethers, protein hydrolysates, vitamins, preservatives, colorants, UV filters, complexing agents, pH adjusters, antioxidants, and viscosity regulators.

[0092] In addition to the cell culture extract and the additional active ingredient, the composition may contain one or more additional anti-dandruff agents. In combination with the cell culture extract described above, the anti-dandruff agent has a particularly strong anti-dandruff effect. The total amount of additional anti-dandruff agents in the composition is preferably 0.01% to 5% by weight, more preferably 0.05% to 3% by weight, further preferably 0.1% to 2% by weight, and most preferably 0.15% to 1% by weight, based on the total weight of the composition.

[0093] Suitable additional anti-dandruff agents include, for example, piroctone olamine, climbazole, zinc pyrithione, ketoconazole, salicylic acid, sulfur, selenium sulfide, tar preparations, undecenic acid derivatives, burdock root extracts, poplar extracts, nettle extracts, walnut shell extracts, birch extracts, willow bark extracts, rosemary extracts, arnica extracts, epsilon polylysine, and propanediol caprylate. Preferred anti-dandruff agents include piroctone olamine, burdock root extracts, poplar extracts, nettle extracts, walnut shell extracts, birch extracts, willow bark extracts, rosemary extracts, arnica extracts, epsilon polylysine, and propanediol caprylate. Particularly preferred anti-dandruff agents are piroctone olamine, epsilon polylysine, and propanediol caprylate. Any mixture of the aforementioned anti-dandruff agents can also be used.

[0094] Polyethylene glycols (PEG) and polypropylene glycols (PPG) are preferably used as polyalkylene glycol ethers.

[0095] The composition preferably comprises 1 wt.% to 25 wt.% polyalkylene glycol ethers, particularly preferably 5 wt.% to 20 wt.%, based on the total weight of the composition.

[0096] The composition preferably includes at least one vitamin suitable for skin care. Suitable vitamins also include provitamins and vitamin precursors. Preferred vitamins are panthenol and niacinamide.

[0097] The formula may also contain a protein hydrolysate. Protein hydrolysates have a conditioning effect on hair and scalp. In this context, protein hydrolysates are complex mixtures of amino acids and oligopeptides obtained by hydrolyzing protein mixtures. A plant-based protein hydrolysate is preferably used.

[0098] Finally, the composition may contain one or more fragrances. Preferably, it is a fragrance composition. The fragrances may be in liquid or encapsulated form.

Claims

[1] Cosmetic composition for the care of hair and scalp comprising a first active ingredient selected from the group consisting of bacitracin, amyloslysine, plantazolicin, bacillomycin D, lactosporin, thuricin CD, bacillaene and mixtures thereof; and a cell culture extract from Bacillus subtilis. [2] Cosmetic composition according to claim 1, wherein the cell culture extract is obtained from the cell-free supernatant of a cell culture fluid. [3] Cosmetic composition according to claim 1 or 2, wherein the cell culture extract is obtained from Bacillus subtilis strains whose 16S ribosomal RNA sequence has at least 97.0% sequence identity to one of the RNA sequences selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO:

4. [4] Cosmetic composition according to one of claims 1 to 3, wherein the cell culture extract comprises the active ingredient bacilysin. [5] Cosmetic composition according to any one of claims 1 to 4, wherein the composition contains 0.1 wt.% to 5 wt.% of the cell culture extract. [6] Cosmetic composition according to any one of claims 1 to 5, wherein the composition contains 0.01 wt.% to 5 wt.% of the first active ingredient. [7] Cosmetic composition according to any one of claims 1 to 6, wherein the composition comprises a surfactant system comprising at least one anionic and at least one amphoteric surfactant. [8] Cosmetic composition according to any one of claims 1 to 6, wherein the composition comprises at least one cationic polymer and / or at least one oil. [9] Cosmetic use of a composition according to any one of claims 1 to 8 for the care of hair and scalp. [10] Cosmetic use according to claim 9 for preventing dandruff on the scalp.