Inhibitor for skin disorder

Abstract

To provide an agent and an external composition capable of effectively inhibiting skin disorder.SOLUTION: The present invention provides an agent for inhibiting inflammation, an agent for scavenging reactive oxygen species, an agent for preventing or inhibiting spots or freckles, or a sunscreen composition, comprising at least one plant extract selected from the group consisting of Belamcanda chinensis De Candolle extract, Salvia officinalis extract, Geranium robertianum L. extract, and Vaccinium myrtillus extract.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an agent and composition for inhibiting skin disorders such as age spots and freckles. [Background technology]

[0002] Skin inflammation is known as a skin disorder caused by ultraviolet radiation. When skin cells are exposed to ultraviolet light, the ultraviolet light, especially UVB, is absorbed by DNA, and adjacent pyrimidine bases bind to form pyrimidine dimers. When pyrimidine dimers are formed, DNA replication is inhibited, so the cells try to repair them. This repair reaction triggers an inflammatory reaction. Inflammation can also occur when damage to the cell membrane caused by ultraviolet light is directly transmitted to the inside of the cell. Inflammation is caused by inflammation-inducing factors that are produced when DNA repair or cell membrane damage is triggered. Inflammation causes the skin to become red and swollen with pain, and after a few days, melanocytes in the epidermis stimulated by the inflammatory reaction produce melanin, which absorbs ultraviolet light, as a defensive reaction. Melanin is taken up by epidermal keratinocytes, but is usually excreted as the epidermal keratinocytes turn over. However, if the inflammatory reaction is strong, a large amount of melanin is produced and enters the dermis, or the turnover of epidermal keratinocytes does not occur normally, resulting in spots. Skin inflammation can be caused by a variety of factors, including external stimuli such as ultraviolet rays, cosmetics, drugs, chemicals, pollen, house dust, viruses, bacteria, fungi, and allergens, as well as internal factors such as sebum secretion and sweating.

[0003] Inflammatory responses involve a variety of inflammation-inducing substances and immune cells, and the responses vary depending on the cause. UVB, which has a short wavelength, contributes to damage to the epidermis, while UVA, which has a long wavelength, contributes to damage to the dermis. It is known that exposure to UV rays, especially UVB, causes the secretion of the primary proinflammatory cytokines IL-1α and TNFα, which further induce the production of IL-6, IL-8, GM-CFS, and SCF, endothelin-1 (ET-1), and prostaglandin E2 (PGE2), which stimulate melanocytes, causing inflammation. In this way, the inflammatory response specific to UV exposure causes pigmentation such as age spots and freckles.

[0004] Problems caused by active oxygen are also known to be skin damage caused by exposure to ultraviolet rays. When exposed to ultraviolet rays, active oxygen is generated, which stimulates melanocytes in the epidermis and promotes melanin production. Melanin is taken up by epidermal keratinocytes, but is usually excreted as the epidermal keratinocytes turn over. However, when exposed to large amounts of ultraviolet rays, excessive active oxygen is generated, causing a large amount of melanin to be produced, and some of the melanin penetrates into the dermis. In addition, the excess active oxygen damages epidermal keratinocytes, preventing normal turnover. This results in spots. In addition, active oxygen damages fibrous proteins such as collagen and elastin present in the dermis, reducing the elasticity of the skin and resulting in wrinkles and sagging. Active oxygen is produced not only by ultraviolet rays, but also by stress, air pollution, smoking, aging, etc., but ultraviolet rays produce large amounts of active oxygen in a short period of time, causing great damage to the skin. Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide an agent and composition capable of effectively suppressing skin damage, particularly an object of the present invention is to provide an agent and composition capable of effectively suppressing skin damage caused by ultraviolet rays. [Means for solving the problem]

[0006] The present inventors have conducted extensive research to solve the above problems and have found that Belamcanda chinensis extract, Sage extract, Geranium globulus extract, and Bilberry extract can suppress inflammation by reducing the amount of inflammatory reaction-inducing substances produced in human epidermal keratinocytes by UV irradiation. They have also found that these plant extracts can eliminate active oxygen in human epidermal keratinocytes. Thus, it has been found that these plant extracts can prevent or suppress skin pigmentation caused by UV exposure.

[0007] The present invention has been completed based on the above findings, and provides the following [1] to

[11] . [1] An inflammation suppressant comprising at least one plant extract selected from the group consisting of Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract. [2] The inflammation suppressant according to [1], wherein the inflammation is caused by ultraviolet light. [3] The inflammation suppressant according to [1] or [2], which suppresses inflammation by reducing the amount of an inflammation-inducing substance. [4] The inflammation suppressant according to any one of [1] to [3], further comprising a mucopolysaccharide. [5] An active oxygen scavenger comprising at least one plant extract selected from the group consisting of bean berry extract, sage extract, gelatinous geranium extract, and bilberry extract. [6] The active oxygen scavenger according to [5], further comprising mucopolysaccharide. [7] An agent for preventing or inhibiting blemishes or freckles, comprising at least one plant extract selected from the group consisting of Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract. [8] The agent for preventing or inhibiting age spots or freckles according to [7], further comprising a mucopolysaccharide. [9] A sunscreen composition comprising at least one plant extract selected from the group consisting of Belamcanda chinensis extract, sage extract, Geranium robertii extract, and bilberry extract.

[10] The sunscreen composition according to [9], wherein the contents of each of the Belamcanda chinensis extract, the sage extract, the Geranium robertii extract, and the bilberry extract are each 0.0001 to 5% by mass relative to the total amount of the composition.

[11] The sunscreen composition according to [9] or

[10] , further comprising a mucopolysaccharide. Effect of the Invention

[0008] Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract can suppress inflammation in human epidermal keratinocytes. For example, when these plant extracts are applied to the epidermis, the production of endothelin-1 and prostaglandin E2, which is increased by ultraviolet (particularly UVB) irradiation, can be suppressed. It is known that endothelin-1 and prostaglandin E2 produced by epidermal keratinocytes act on inflammatory cells to induce an inflammatory reaction, which causes skin pigmentation. Therefore, these plant extracts can suppress the inflammatory reaction caused by ultraviolet irradiation in advance, and as a result, pigmentation such as age spots and freckles caused by the inflammatory reaction can be prevented or suppressed. In addition, these plant extracts can eliminate reactive oxygen species in human epidermal keratinocytes, and thus can suppress melanin production caused by reactive oxygen species, thereby preventing or suppressing pigmentation such as age spots and freckles caused by reactive oxygen species. [Brief description of the drawings]

[0009] [Figure 1] FIG. 13 is a graph showing that Belamcanda chinensis extract, sage extract, Geranium globulus extract, and sodium hyaluronate suppressed the increase in prostaglandin E2 caused by UVB irradiation in human epidermal keratinocytes. [Diagram 2] FIG. 13 is a graph showing that Belamcanda chinensis extract, sage extract, and bilberry extract suppressed the increase in endothelin-1 caused by UVB irradiation in human epidermal keratinocytes. [Diagram 3]FIG. 1 is a graph showing that Belamcanda chinensis extract, sage extract, bilberry extract, and Geranium globulus extract reduced the amount of active oxygen in human epidermal keratinocytes. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The present invention will be described in detail below. Purpose As described above, Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract (hereinafter sometimes referred to as "plant extracts of the present invention") have the effect of suppressing the production of inflammation-inducing substances in human epidermal keratinocytes, and therefore can be used as active ingredients in agents for preventing or suppressing inflammation in human skin (particularly, human epidermis). Inflammatory responses involve a variety of inflammation-inducing substances and a variety of immune cells, and the responses vary depending on the cause. The plant extract of the present invention can be used as an active ingredient of an agent for preventing or suppressing inflammation caused in human skin (particularly human epidermis) by ultraviolet rays (particularly UVB). It can also be used as an active ingredient of an agent for reducing the amount of inflammation-inducing substances produced in human skin (particularly human epidermis) by ultraviolet rays (particularly UVB). Examples of inflammation-inducing substances include endothelin-1 and prostaglandin E2. The plant extract of the present invention can also be used as an active ingredient of an agent for suppressing the initiation of inflammation caused in human skin (particularly human epidermis) by ultraviolet rays (particularly UVB).

[0011] In addition, since the plant extract of the present invention has the ability to scavenge active oxygen in human epidermal keratinocytes, it can be used as an active ingredient of an active oxygen scavenger. In particular, it can be used as an active ingredient of an active oxygen scavenger for human skin (particularly human epidermis). In particular, it can be used as an active ingredient of an active oxygen scavenger for the active oxygen generated in human skin (particularly human epidermis) by irradiation with ultraviolet light (particularly UVB).

[0012] Based on these actions, the plant extract of the present invention can be used as an active ingredient in a preventive or suppressive agent for pigmentation (age spots, freckles, etc.), particularly for pigmentation (age spots, freckles, etc.) caused by ultraviolet rays (particularly UVB). It can also be used as an active ingredient in a sunscreen. "Sunscreen" means that it is unlikely to cause sunburn or does not cause sunburn even when exposed to ultraviolet rays.

[0013] In the present invention, "prevention" includes preventing the symptoms from occurring at all by suppressing them in advance, and alleviating or reducing the symptoms by suppressing them in advance.

[0014] Belamcanda chinensis extract The Belamcanda chinensis extract is an extract of Belamcanda chinensis De Candolle. The extract may be from any part of Belamcanda chinensis, but an extract from the rhizome is preferred. The extraction solvent may be any solvent used in the field of cosmetics for the extraction of plant components, and examples of the extraction solvent include hydrophilic or polar solvents such as water; aqueous solutions of inorganic salts such as sodium chloride, potassium chloride, magnesium chloride, and ammonium carbonate; buffer solutions such as phosphate buffer, acetate buffer, Tris-HCl buffer, carbonate buffer, and borate buffer; aqueous solutions of inorganic acids such as hydrochloric acid, carbonic acid, sulfuric acid, nitric acid, and phosphoric acid; aqueous solutions of organic acids such as acetic acid, citric acid, lactic acid, succinic acid, ascorbic acid, fumaric acid, and malic acid; aqueous solutions of surfactants such as saponin and lecithin; lower alcohols such as methanol, ethanol, propanol, and butanol; ketones such as acetone and ethyl methyl ketone; and polyhydric alcohols such as butylene glycol (particularly 1,3-butylene glycol), glycerin, and propylene glycol. Other examples of the extraction solvent include hydrophobic solvents such as hydrocarbons such as hexane and cyclohexane; ethers such as diethyl ether; and acetate esters such as ethyl acetate and methyl acetate. The solvent may be used alone or in combination of two or more. Among these, hydrophilic solvents are preferred because they are capable of extracting components that have strong active oxygen scavenging properties and anti-inflammatory properties caused by ultraviolet irradiation, and mixtures of water, lower alcohols, and polyhydric alcohols are more preferred, with mixtures of water, ethanol, and butylene glycol (particularly 1,3-butylene glycol) being particularly preferred. Commercially available Belamcanda camara extracts include Belamcanda camara extracts (particularly Belamcanda camara rhizome extracts) extracted with a mixture of water, ethanol, and 1,3-butylene glycol, and containing an aqueous solution of 20% by mass ethanol and 20% by mass 1,3-butylene glycol.

[0015] Sage Extract The sage extract is an extract of sage (Salvia officinalis). The extract may be from any part of the sage, but an extract of the flowers, leaves or whole plant is preferred. Examples of extraction solvents that can be used include those exemplified for Belamcanda chinensis extract, but among them, hydrophilic solvents are preferred, polyhydric alcohol solutions (particularly aqueous polyhydric alcohol solutions) are more preferred, butylene glycol solutions (particularly aqueous butylene glycol solutions) are more preferred, and 1,3-butylene glycol solutions (particularly aqueous 1,3-butylene glycol solutions) are particularly preferred. Commercially available sage extracts include sage extracts extracted with a 1,3-butylene glycol solution and containing 50% by mass of 1,3-butylene glycol aqueous solution (particularly, an extract of one or more of the whole plant, leaves, and flowers of sage).

[0016] Geranium extract The Geranium robertianum extract is an extract of Geranium robertianum L. The extract may be from any part of Geranium robertianum L., but an extract of the whole plant is preferred. Examples of extraction solvents that can be used include those exemplified for Belamcanda chinensis extract, but among them, hydrophilic solvents are preferred, polyhydric alcohol solutions (particularly aqueous polyhydric alcohol solutions) are more preferred, butylene glycol solutions (particularly aqueous butylene glycol solutions) are more preferred, and 1,3-butylene glycol solutions (particularly aqueous 1,3-butylene glycol solutions) are particularly preferred. Commercially available products of Geranium Geranium extract include Geranium Geranium extract extracted with 1,3-butylene glycol solution and containing 50% by mass of 1,3-butylene glycol aqueous solution.

[0017] Bilberry Extract The bilberry extract is an extract of bilberry (Vaccinium myrtillus). It may be an extract of the whole plant, leaves, flowers, stems, rhizomes, roots, etc. of bilberry, but an extract of leaves is preferred. Examples of extraction solvents that can be used include those exemplified for Belamcanda chinensis extract, but among them, hydrophilic solvents are preferred, polyhydric alcohol solutions (particularly aqueous polyhydric alcohol solutions) are more preferred, butylene glycol solutions (particularly aqueous butylene glycol solutions) are more preferred, and 1,3-butylene glycol solutions (particularly aqueous 1,3-butylene glycol solutions) are particularly preferred. Commercially available bilberry extracts include those extracted with a 1,3-butylene glycol solution and containing a 50% by mass aqueous 1,3-butylene glycol solution.

[0018] Mucopolysaccharides Each agent of the present invention can contain one or more kinds of mucopolysaccharides as active ingredients in addition to Belamcanda chinensis extract, sage extract, Geranium globulus extract, and / or bilberry extract, which can significantly or synergistically suppress the inflammatory reaction caused by ultraviolet radiation and suppress blemishes and freckles. Examples of mucopolysaccharides include heparin-like substances; chondroitin sulfates or salts thereof, such as chondroitin sulfate and sodium chondroitin sulfate; dermatan sulfates or salts thereof, such as dermatan sulfate and sodium dermatan sulfate; hyaluronic acid or salts thereof; heparan sulfate; heparin; keratan sulfate I and II; and the like.

[0019] Of these, hyaluronic acid or a salt thereof is preferred. The salt of hyaluronic acid can be any pharmaceutical or physiologically acceptable salt, and can be, for example, the salt with organic base (organic amine salt such as methylamine salt, triethylamine salt, triethanolamine salt, morpholine salt, piperazine salt, pyrrolidine salt, tripyridine salt, picoline salt, etc.), the salt with inorganic base (ammonium salt; alkali metal salt such as sodium salt, potassium salt, alkaline earth metal salt such as calcium salt, magnesium salt, metal salt such as zinc salt, aluminum salt, etc.).Among these salts, the salt with inorganic base is preferred, alkali metal salt is more preferred, and sodium salt is particularly preferred. When the agent of the present invention contains a hyaluronate, it may be a formulation containing a hyaluronate, or it may be a formulation in which hyaluronic acid and an organic or inorganic base are separately blended to form a hyaluronate in the formulation.

[0020] The average molecular weight of hyaluronic acid or a salt thereof is not particularly limited, but from the viewpoint of achieving the effects of the present invention, it can be 500,000 or more, 600,000 or more, 700,000 or more, 800,000 or more, 900,000 or more, or 1 million or more, and can be 10 million or less, 5 million or less, 4.5 million or less, 4 million or less, 3.5 million or less, 3 million or less, 2.5 million or less, 2 million or less, or 1.6 million or less.

[0021] The average molecular weight of hyaluronic acid or a salt thereof is a value measured by the method described in the section on "purified sodium hyaluronate" in the 18th Edition of the Japanese Pharmacopoeia.

[0022] formulation The formulation form of each agent of the present invention includes liquids, suspensions, emulsions, creams, emulsion ointments, ointments, gels, liniments, lotions, foams, sprays, aerosols, pump foams, sheets such as nonwoven fabrics impregnated with a drug solution, and sticks. In the case of emulsion compositions such as emulsions, creams, and emulsion ointments, they may be either oil-in-water or water-in-oil. Among these, the oil-in-water type is preferred. Each agent of the present invention can be used as an external agent, particularly an external agent for the skin, and can also be formulated as a cosmetic or quasi-drug.

[0023] The concentration of each of the Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract in the formulation can be 0.0001% by mass or more, 0.001% by mass or more, 0.01% by mass or more, or 0.1% by mass or more, based on the total amount of the formulation. Within this range, the effects of these plant extracts can be sufficiently obtained. Also, it can be 5% by mass or less, 1% by mass or less, or 0.1% by mass or less. When the extract contains a solvent, the concentration of this plant extract is a value calculated based on the mass including the solvent.

[0024] In addition, the concentration of each of the Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract in the preparation can be 0.000001% by mass or more, 0.00001% by mass or more, 0.0001% by mass or more, 0.001% by mass or more, or 0.01% by mass or more, calculated as dry weight, based on the total amount of the preparation. Within this range, the effects of these plant extracts can be sufficiently obtained. In addition, the concentration can be 0.05% by mass or less, 0.01% by mass or less, or 0.001% by mass or less.

[0025] When mucopolysaccharides are added, their total concentration can be 0.00001% by mass or more, 0.00005% by mass or more, 0.0001% by mass or more, 0.0005% by mass or more, or 0.001% by mass or more, based on the total amount of the preparation. Within this range, the effects of mucopolysaccharides can be sufficiently obtained. In addition, the total concentration can be 5% by mass or less, 2% by mass or less, 1% by mass or less, 0.5% by mass or less, 0.1% by mass or less, 0.05% by mass or less, or 0.01% by mass or less.

[0026] When mucopolysaccharides are incorporated, the total amount may be 0.0001 parts by mass or more, 0.01 parts by mass or more, 0.1 parts by mass or more, or 1 part by mass or more per part by mass of the total plant extracts of the present invention (the total of at least one selected from Belamcanda chinensis extract, sage extract, Geranium robertii extract, and bilberry extract), and may be 100 parts by mass or less, 10 parts by mass or less, 1 part by mass or less, or 0.1 parts by mass or less. Within this range, the effects of mucopolysaccharides can be sufficiently obtained.

[0027] When mucopolysaccharides are incorporated, the total amount may be 0.000001 parts by mass or more, 0.0001 parts by mass or more, 0.001 parts by mass or more, or 0.01 parts by mass or more, and may be 1 part by mass or less, 0.1 parts by mass or less, 0.01 parts by mass or less, or 0.001 parts by mass or less, relative to 1 part by mass of the total dry weight of the plant extracts of the present invention (the total of at least one selected from Belamcanda chinensis extract, sage extract, Geranium robertii extract, and bilberry extract).Within this range, the effects of mucopolysaccharides can be sufficiently obtained.

[0028] In addition to the plant extract and mucopolysaccharides of the present invention, the preparation may contain bases, additives, and other physiologically or pharmacologically active ingredients (other than the plant extract and mucopolysaccharides of the present invention) used in cosmetics and quasi-drugs.

[0029] Base The base includes an oily base and an aqueous base. Oily bases include hydrocarbons such as petrolatum (white petrolatum, yellow petrolatum), gelling hydrocarbons (such as Plastibase), ozokerite, ceresin, microcrystalline wax, squalene, squalane, α-olefin oligomers, paraffin, liquid paraffin, and light liquid paraffin; higher alcohols such as cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol; sterols such as cholesterol, phytosterol, and phytosteryl hydroxystearate; shea butter, Vegetable fats such as carnauba wax, cocoa butter, and candelilla wax; vegetable oils such as avocado oil, olive oil, camellia oil, macadamia nut oil, evening primrose oil, jojoba oil, rapeseed oil, egg yolk oil, sesame oil, castor oil, safflower oil, cottonseed oil, soybean oil, tea seed oil, rice bran oil, rice germ oil, wheat germ oil, peanut oil, sunflower oil, almond oil, corn oil, coconut oil, orange oil, sage oil, palm oil, mink oil, meadowfoam oil, lavender oil, rosemary oil, and rosehip oil; lanolin, orange roughy oil, squalane, horse oil, spermaceti, and and animal fats and oils such as beeswax; hydrogenated oils; silicone oils such as methylpolysiloxane, crosslinked methylpolysiloxane, highly polymerized methylpolysiloxane, cyclic silicone, alkyl-modified silicone, crosslinked alkyl-modified silicone, amino-modified silicone, polyether-modified silicone, polyglycerin-modified silicone, crosslinked polyether-modified silicone, crosslinked alkyl polyether-modified silicone, silicone-alkyl chain co-modified polyether-modified silicone, silicone-alkyl chain co-modified polyglycerin-modified silicone, polyether-modified branched silicone, polyglycerin-modified branched silicone, acrylic silicone, phenyl-modified silicone, and silicone resin; natural polymer derivatives such as ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, and cationized guar gum; synthetic polymers such as polyvinylpyrrolidone, carboxyvinyl polymer, and alkyl acrylate / methacrylate copolymer; natural polymers such as carrageenan, alginic acid, cellulose, guar gum, quince seed, dextran, and gellan gum;Esters such as diethylhexyl succinate, isopropyl myristate, octyldodecyl myristate, isopropyl palmitate, cetyl palmitate, isononyl isononanoate, pentaerythritol tetra 2-ethylhexanoate, and caprylic / capric triglyceride; polysaccharides such as dextrin and maltodextrin; glycol ethers such as ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, diethylene glycol monobutyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, dipropylene glycol monoethyl ether, and dipropylene glycol monopropyl ether; In addition to water and buffer solutions, aqueous bases include lower alcohols such as ethanol and isopropanol; and polyhydric alcohols such as polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, diglycerin, and isoprene glycol. One or more bases may be used.

[0030] Examples of additives include antioxidants, surfactants, thickeners, pH adjusters, stabilizers, chelating agents, UV absorbers, UV scattering agents, irritation reducers, colorants, and fragrances. The additives may be used alone or in combination of two or more. Moreover, additives can be used within a range that does not impair the effects of the present invention.

[0031] Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, p-hydroxyanisole, sorbic acid, sodium sulfite, ascorbic acid, ascorbic acid derivatives (ascorbic acid stearate, ascorbic acid palmitate, ascorbyl dipalmitate, ascorbic acid monophosphate, ascorbic acid diphosphate, ascorbic acid triphosphate, ascorbic acid sulfate, etc.), tocopherol, tocopherol derivatives (tocopherol acetate, tocopherol succinate, tocopherol calcium succinate, etc.), erythorbic acid, L-cysteine ​​hydrochloride, lycopene, glutathione, propyl gallate, tannic acid, epigallocatechin, anthocyanin, hydroxytyrosol, nordihydroguaiaretic acid, caffeic acid, and enzymes (catalase, superoxide dismutase, glutathione peroxidase, elastase, etc.).

[0032] Surfactants include POE-2-decyltetradecyl alcohol, POE-alkyl ethers such as polyoxyethylene oleyl ether and polyoxyethylene cetyl ether; sorbitan fatty acid esters such as sorbitan monostearate, sorbitan sesquistearate, sorbitan tristearate, and sorbitan monooleate; POE(20) sorbitan monoisostearate, POE(20) sorbitan monooleate, POE(20) sorbitan monostearate, POE(20) sorbitan monococoate, and sorbitan lauroyl esters. POE-sorbitan fatty acid esters such as POE(80) sorbitan urate; glycerin fatty acid esters such as glyceryl monostearate and glyceryl oleate; POE-glycerin fatty acid esters such as polyoxyethylene glyceryl stearate and polyoxyethylene glyceryl trioleate; POE-dihydrocholesterol ester, POE-hydrogenated castor oil (e.g., polyoxyethylene hydrogenated castor oil 40 (HCO-40), polyoxyethylene hydrogenated castor oil 50 (HCO-50), polyoxyethylene hydrogenated castor oil POE-hydrogenated castor oils such as polyoxyethylene hydrogenated castor oil 80 (HCO-60), polyoxyethylene hydrogenated castor oil 80, etc.; POE-castor oils such as POE(3) castor oil, POE(20) castor oil, etc.; POE-hydrogenated castor oil fatty acid esters such as POE-hydrogenated castor oil isostearate; POE-alkylaryl ethers; glycerin alkyl ethers such as cumyl alcohol, batyl alcohol, and selachyl alcohol; POE-glycerin alkyl ethers such as POE-monostearyl glyceryl ether; polystearate POE fatty acid esters such as lyoxyl; POE sterols and hydrogenated sterols such as POE(20) phytosterol, POE(30) phytosterol, POE(25) phytostanol, and POE(30) cholestanol; sucrose fatty acid esters such as sucrose palmitate, sucrose stearate, sucrose oleate, sucrose isostearate, sucrose linoleate, and sucrose linolenate; polyethylene glycol fatty acid esters such as polyethylene glycol dioleate;Examples of such surfactants include polyglycerin fatty acid esters such as alkyl polyglycosides, diglyceryl monostearate, diglyceryl monoisostearate, decaglyceryl monostearate, decaglyceryl monooleate, decaglyceryl pentaoleate, and hexaglyceryl polyricinoleate; and nonionic surfactants such as alkyl-modified silicones and emulsifying silicone elastomers. Also, higher alkyl sulfate ester salts (e.g., sodium lauryl sulfate, potassium lauryl sulfate, etc.); alkyl ether sulfate ester salts (e.g., POE-lauryl triethanolamine sulfate, sodium POE-lauryl sulfate, etc.); N-acylsarcosinic acid (e.g., sodium lauroyl sarcosinate, etc.); higher fatty acid amide sulfonates (e.g., sodium stearoyl methyl taurine, sodium myristoyl methyl taurine, sodium cocoyl methyl taurine, sodium cocoyl methyl taurine taurine, etc.); phosphate esters and their salts (sodium POE-oleyl ether phosphate, POE-stearyl ether phosphate, potassium cetyl phosphate, cetyl phosphate, etc.); sulfosuccinates (e.g., sodium di-2-ethylhexyl sulfosuccinate, etc.); alkyl benzene sulfonates (e.g., sodium linear dodecyl benzene sulfonate, etc.) and the like); higher fatty acid ester sulfate salts (e.g., sodium hydrogenated coconut oil fatty acid glycerin sulfate); N-acyl glutamate salts (e.g., monosodium lauroyl glutamate, sodium stearoyl glutamate, sodium myristoyl glutamate, etc.); N-acylalanine salts (e.g., sodium lauroyl methyl alanine, cocoyl alanine triethanolamine salt, etc.); sulfated oils (e.g., turmeric oil, etc.); POE-alkyl ether carboxylic acids; POE-alkyl allyl ether carboxylate salts; α-olefin sulfonates; higher fatty acid ester sulfonates; secondary alcohol sulfate salts; higher fatty acid alkylolamide sulfate salts; sodium lauroyl monoethanolamide succinate; N-palmitoyl aspartic acid ditriethanolamine; and anionic surfactants such as sodium caseinate. Further examples include cationic surfactants such as mono- or di-long-chain alkyl tertiary or quaternary ammonium salts having a straight-chain or branched long-chain alkyl group to which an alkylene oxide may be added. Also included are amphoteric surfactants such as carbobetaines, sulfobetaines, imidazolinium betaines, and amidobetaines. Other examples include naturally occurring surfactants such as lecithin, hydrogenated lecithin, saponin, sodium surfactin, and bile acid.

[0033] Examples of thickeners include guar gum, locust bean gum, carrageenan, xanthan gum, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / alkyl methacrylate copolymer, polyethylene glycol, bentonite, alginic acid, macrogol, and cellulose-based thickeners (methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, etc.).

[0034] Examples of pH adjusters include inorganic acids (such as hydrochloric acid and sulfuric acid), organic acids (such as lactic acid, sodium lactate, citric acid, sodium citrate, succinic acid, and sodium succinate), inorganic bases (such as potassium hydroxide and sodium hydroxide), and organic bases (such as triethanolamine, diisopropanolamine, and triisopropanolamine).

[0035] The stabilizer includes sodium polyacrylate, dibutylhydroxytoluene, butylhydroxyanisole, and the like.

[0036] Chelating agents include EDTA disodium salt and EDTA calcium disodium salt.

[0037] Irritation reducers include licorice extract, sodium alginate, etc.

[0038] UV absorbers include 2-ethylhexyl paramethoxycinnamate, 2-[4-(diethylamino)-2-hydroxybenzoyl]benzoic acid hexyl ester, methylene bisbenzotriazolyl tetramethylbutylphenol, bisethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, 2,4,6-tris[4-(2-ethylhexyloxycarbonyl)anilino]-1,3,5-triazine, and dimethoxybenzylidene oxoimidazolidine proline. Examples of such compounds include 2-ethylhexyl pionate, 2,4-bis-[{4-(2-ethylhexyloxy)-2-hydroxy}-phenyl]-6-(4-methoxyphenyl)-1,3,5-triazine, t-butyl methoxydibenzoylmethane, dibenzylidene dioxoimidazolidine ethylhexyl propylonate, ethoxyhexyl triazoline, para-aminobenzoic acid and its derivatives, octyl para-dimethylaminobenzoate, ethylene glycol salicylate, and dihydroxybenzophenone. Among these, 2-ethylhexyl paramethoxycinnamate, 2-[4-(diethylamino)-2-hydroxybenzoyl]benzoic acid hexyl ester, methylene bisbenzotriazolyl tetramethylbutylphenol, bisethylhexyloxyphenol methoxyphenyl triazine, and ethylhexyl triazone are preferred.

[0039] Examples of ultraviolet scattering agents include inorganic compounds such as zinc oxide, titanium oxide, iron oxide, cerium oxide, zirconium oxide, titanium silicate, zinc silicate, anhydrous silicic acid, cerium silicate, and hydrous silicic acid; these inorganic compounds coated with hydrous silicic acid, aluminum hydroxide, or inorganic powder such as mica or talc; these inorganic compounds composited with resin powder such as polyamide, polyethylene, polyester, polystyrene, or nylon; and these inorganic compounds treated with silicone oil, aluminum salt of a fatty acid, or the like.

[0040] Examples of coloring agents include the dyes listed in the Legal Dyes Handbook (edited by the Japan Cosmetic Industry Association (2004)).

[0041] Examples of the fragrance include various essential oils such as herb-based essential oils, such as lavender oil, rosemary oil, clary sage oil, thyme oil, bergamot oil, and eucalyptus oil, citrus-based essential oils, such as orange oil, lemon oil, and grapefruit oil, and blended fragrances.

[0042] Other physiologically active or pharmacologically active ingredients (other than the plant extract and mucopolysaccharides of the present invention) include moisturizing ingredients, anti-inflammatory ingredients, antibacterial ingredients, vitamins, peptides or their derivatives, amino acids or their derivatives, cell activating ingredients, anti-aging ingredients, blood circulation promoting ingredients, keratin softening ingredients, whitening ingredients, astringent ingredients, etc. As for other physiologically active or pharmacologically active ingredients, one or more kinds can be used. In addition, other physiologically or pharmacologically active ingredients can be used within the scope that does not impair the effects of the present invention.

[0043] The plant extract and mucopolysaccharides of the present invention may exhibit the functions of the physiologically active or pharmacologically active ingredients listed above, but examples of physiologically active or pharmacologically active ingredients other than the plant extract and mucopolysaccharides of the present invention are given below.

[0044] Moisturizing ingredients include lipids such as ceramide, cholesterol, and phospholipids; plant extracts such as chamomile extract, mulberry extract, Houttuynia cordata extract, carrot extract, witch hazel extract, tea extract, and perilla extract; polymeric compounds such as collagen, elastin, keratin, chitin, and chitosan; polyhydric alcohols such as glycerin, 1,3-butylene glycol, propylene glycol, polyethylene glycol, and diglycerin trehalose; amino acids such as alanine, serine, leucine, isoleucine, threonine, glycine, proline, hydroxyproline, glucosamine, aspartic acid, theanine, and arginine; natural moisturizing factors such as sodium lactate, urea, and sodium pyrrolidone carboxylate; sugar alcohols such as sorbitol; phospholipids such as lecithin and hydrogenated lecithin; polyglutamic acid; polyoxypropylene methyl glucoside; trimethylglycine (betaine); hydroxyethyl urea; acrylic acid-acrylamide-dimethyl diallyl ammonium chloride copolymer, etc.

[0045] Anti-inflammatory ingredients include allantoin, glycyrrhizic acid or its derivatives, zinc oxide, pyridoxine hydrochloride, salicylic acid or its derivatives, ε-aminocaproic acid, proanthocyanidin, tocopherol or its derivatives, ascorbic acid or its derivatives, hesperidin, glucosyl hesperidin, ergothioneine, sodium bisulfite, erythorbic acid or its salts, glutathione, glutathione peroxidase, glutathione-S-transferase, catalase, superoxide dismutase, thioredoxin, taurine, thiotaurine, hypotaurine, and the like.

[0046] Examples of antibacterial ingredients include chlorhexidine, salicylic acid, quaternary ammonium salt antibacterial ingredients (benzalkonium chloride, benzethonium chloride, etc.), acrinol, sulfur, resorcinol, ethanol, adapalene, benzoyl peroxide, clindamycin, cresol, gluconic acid and its derivatives, povidone-iodine, potassium iodide, iodine, isopropylmethylphenol, triclocarban, triclosan, photosensitizer No. 101, photosensitizer No. 201, paraben, phenoxyethanol, alkanediols such as 1,2-pentanediol, glycerin fatty acid esters, azelaic acid, alkyldiaminoglycine hydrochloride, chlorhexidine gluconate, and zinc paraphenolsulfonate.

[0047] Vitamins include retinol derivatives such as retinol, retinol acetate, and retinol palmitate, retinal, retinoic acid, methyl retinoate, ethyl retinoate, retinol retinoate, d-δ-tocopheryl retinoate, α-tocopheryl retinoate, and β-tocopheryl retinoate; vitamin A; β-carotene, α-carotene, γ-carotene, δ-carotene, lycopene, zeaxanthin, cryptoxanthin, and the like. Provitamin A such as echinenone and δ-tocopherol, dl-α-tocopherol, dl-α-tocopherol acetate, dl-α-tocopherol succinate, dl-α-tocopherol calcium succinate, tocopherol nicotinate, and other vitamin E derivatives; riboflavin, flavin mononucleotide, flavin adenine dinucleotide, riboflavin butyrate, riboflavin tetrabutyrate, and riboflavin 5'-phosphate ester. Vitamin B2 such as sodium nicotinate and riboflavin tetranicotinate; nicotinic acids such as dl-α-tocopherol nicotinate, benzyl nicotinate, methyl nicotinate, β-butoxyethyl nicotinate, and 1-(4-methylphenyl)ethyl nicotinate; vitamin C such as ascorbyl stearate, L-ascorbyl dipalmitate, ascorbyl tetraisopalmitate (ascorbyl tetra-2-hexyldecanoate), ascorbic acid, sodium ascorbate, dehydroascorbic acid, sodium ascorbyl phosphate, magnesium ascorbyl phosphate, ascorbyl glucoside, ascorbigen-A, ascorbyl stearate, and ascorbyl palmitate; vitamin D such as methylhesperidin, ergocalciferol, and cholecalciferol; vitamin K such as phylloquinone and farnoquinone;Vitamin B1 such as dibenzoyl thiamine, dibenzoyl thiamine hydrochloride, thiamine hydrochloride, thiamine cetyl hydrochloride, thiamine thiocyanate, thiamine lauryl hydrochloride, thiamine nitrate, thiamine monophosphate, thiamine lysine salt, thiamine triphosphate, thiamine monophosphate ester, thiamine diphosphate ester, thiamine diphosphate ester hydrochloride, thiamine triphosphate ester, thiamine triphosphate ester monophosphate; pyridoxine hydrochloride, pyridoxine acetate, pyridoxal hydrochloride, pyridoxal 5'-phosphate, pyridoxal hydrochloride Vitamin B6 such as cyanocobalamin, hydroxocobalamin, deoxyadenosylcobalamin, vitamin B12 such as cyanocobalamin, hydroxocobalamin, deoxyadenosylcobalamin, folic acids such as folic acid and pteroylglutamic acid, nicotinic acids such as nicotinic acid and nicotinamide, pantothenic acids such as pantothenic acid, calcium pantothenate, pantothenyl alcohol (panthenol), D-panthesine, D-pantethine, coenzyme A, pantothenyl ethyl ether, biotins such as biotin and bioticin, vitamin-like action factors such as γ-oryzanol, carnitine, ferulic acid, α-lipoic acid, and orotic acid.

[0048] Examples of peptides or derivatives thereof include keratin hydrolyzed peptides, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin hydrolyzed peptides, collagen hydrolyzed peptides, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin hydrolyzed peptides, conchiolin hydrolyzed peptides, hydrolyzed conchiolin, silk proteolytic peptides, hydrolyzed silk, sodium lauroyl hydrolyzed silk, soybean proteolytic peptides, hydrolyzed soybean protein, wheat protein, wheat proteolytic peptides, hydrolyzed wheat protein, casein hydrolyzed peptides, and acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.).

[0049] Examples of amino acids or derivatives thereof include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosine, and creatine.

[0050] Cell activation components include amino acids such as gamma-aminobutyric acid and epsilon-aminopropionic acid; vitamins such as retinol, thiamine, riboflavin, pyridoxine hydrochloride, and pantothenic acids; alpha-hydroxy acids such as glycolic acid and lactic acid; tannins; flavonoids; saponin; allantoin; and photosensitizer No. 301.

[0051] Anti-aging ingredients include pangamic acid, kinetin, ursolic acid, turmeric extract, sphingosine derivatives, silicon, silicic acid, N-methyl-L-serine, and mevalonolactone.

[0052] Examples of blood circulation-promoting ingredients include ingredients derived from plants (e.g., ginseng, angelica, arnica, ginkgo, fennel, Japanese oak, Dutch oak, chamomile, Roman chamomile, carrot, gentian, burdock, rice, hawthorn, shiitake mushroom, European hawthorn, European juniper, Cnidium gypsum, Swertia japonica, thyme, clove, tangerine peel, angelica tree, peach kernel, spruce, carrot, garlic, butcher's broom, grape, peony, horse chestnut, melissa, yuzu, coix seed, rosemary, rose hip, tangerine peel, angelica tree, spruce, peach, apricot, walnut, or corn); glucosyl hesperidin, and the like.

[0053] Examples of keratin softening ingredients include urea, salicylic acid, glycolic acid, gluconic acid, fruit acid, phytic acid, lanolin, lactic acid, lactate salts, citric acid, and sulfur.

[0054] Whitening ingredients include arbutin, hydroquinone, kojic acid, ellagic acid, phytic acid, rucinol, chamomilla ET, ascorbic acid or a derivative thereof, vitamin E or a derivative thereof, pantothenic acid or a derivative thereof, and the like.

[0055] Examples of astringent ingredients include metal salts such as alum, chlorohydroxyaluminum, aluminum chloride, aluminum allantoin salt, zinc sulfate, zinc paraphenolsulfonate, zinc oxide, and potassium aluminum sulfate; organic acids such as tannic acid, citric acid, lactic acid, and succinic acid; menthol, and ethanol.

[0056] The pH of each agent of the present invention can be 5 or more, 5.5 or more, 6 or more, 6.5 or more, or 7 or more, and can be 8.5 or less, 8 or less, 7.5 or less, 7 or less, 6.5 or less, or 6 or less.

[0057] Each of the agents of the present invention can be used as a skin care cosmetic, a makeup base, a make-up cosmetic, a sunscreen cosmetic, and the like. As shown in the examples, the plant extract of the present invention reduces the amount of inflammation-inducing substances produced by UV irradiation, thereby preventing the onset of inflammatory reactions and therefore the production of melanin, and is therefore preferably applied to the skin before exposure to UV rays, which can effectively prevent pigmentation caused by UV rays. Therefore, it is preferable to use each agent of the present invention as a sunscreen cosmetic, a makeup base, or a make-up cosmetic, and apply it to the skin in advance before going out and being exposed to the sun.

[0058] Sunscreen composition The sunscreen composition of the present invention is a composition containing at least one plant extract selected from the group consisting of Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract. "Sunscreen" means that it is unlikely to cause sunburn or does not cause sunburn even when exposed to ultraviolet rays. In this sunscreen composition, the plant extract of the present invention may be blended for the purpose of sunscreening, or may be blended for purposes other than sunscreening.

[0059] The concentration of each of the Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract in the composition can be 0.0001% by mass or more, 0.001% by mass or more, 0.01% by mass or more, 0.1% by mass or more, or 1% by mass or more, and can be 5% by mass or less, 1% by mass or less, 0.1% by mass or less, or 0.01% by mass or less, based on the total amount of the formulation. When the extract contains a solvent, the concentration of the plant extract of the present invention is a value calculated based on the mass including the solvent.

[0060] In addition, the concentration of each of the bean berry extract, sage extract, gelatinous geranium extract, and bilberry extract in the sunscreen composition can be 0.000001% by mass or more, 0.00001% by mass or more, 0.0001% by mass or more, 0.001% by mass or more, or 0.01% by mass or more, based on the total amount of the composition, calculated as dry weight. Within this range, the effects of these plant extracts can be sufficiently obtained. In addition, the concentration can be 0.05% by mass or less, 0.01% by mass or less, or 0.001% by mass or less.

[0061] The sunscreen composition of the present invention may further comprise one or more mucopolysaccharides. When mucopolysaccharides are included, their total concentration can be 0.00001 mass% or more, 0.00005 mass% or more, 0.0001 mass% or more, 0.0005 mass% or more, or 0.001 mass% or more, and can be 5 mass% or less, 2 mass% or less, 1 mass% or less, 0.5 mass% or less, 0.1 mass% or less, 0.05 mass% or less, or 0.01 mass% or less, relative to the total amount of the composition.

[0062] When mucopolysaccharides are incorporated, the total amount may be 0.0001 parts by mass or more, 0.001 parts by mass or more, 0.01 parts by mass or more, 0.1 parts by mass or more, or 1 part by mass or more relative to 1 part by mass of the total plant extracts of the present invention (the total of at least one selected from Belamcanda chinensis extract, sage extract, Geranium robertii extract, and bilberry extract), and may be 100 parts by mass or less, 10 parts by mass or less, 1 part by mass or less, or 0.1 parts by mass or less. Within this range, the effects of mucopolysaccharides can be sufficiently obtained.

[0063] The amount may be 0.000001 parts by mass or more, 0.0001 parts by mass or more, 0.001 parts by mass or more, or 0.01 parts by mass or more, and may be 1 part by mass or less, 0.1 parts by mass or less, 0.01 parts by mass or less, or 0.001 parts by mass or less, relative to 1 part by mass of the total dry weight of the plant extracts of the present invention (the total of at least one selected from Belamcanda chinensis extract, sage extract, Geranium robertii extract, and bilberry extract).Within this range, the effect of the mucopolysaccharides can be sufficiently obtained.

[0064] In addition to the plant extract and mucopolysaccharide of the present invention, the composition may contain bases, additives, and other physiologically active ingredients used in cosmetics and quasi-drugs.

[0065] The extraction solvent and plant part of the plant extract of the present invention, the type and molecular weight of mucopolysaccharide, type of base, type of additive, type of physiologically active or pharmacologically active ingredient, formulation form, pH, application (skin care cosmetic, makeup base, make-up cosmetic, etc.), method of use, etc. are the same as those explained for each agent of the present invention.

[0066] The sunscreen composition of the present invention may be a composition packed in a container. Examples of the container include a bottle container, a tube container, a jar container, a dropper container, a dispenser container, a pouch bag, a cheer pack, a sponge head container, a roll-on container, a stick container, etc. EXAMPLES

[0067] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these.

[0068] (1) Evaluation of anti-inflammatory effect (reduction of prostaglandin E2) On the first day, human normal epidermal keratinocytes (NHEK) (KURABO KK-4009) were cultured in a 6-well plate (Corning 3516 plate) at 5 × 10 5 The cells were seeded at 1000 cells / well and cultured in an incubator at 37°C with 5% CO2. Humedia KG2 medium contains all the additives listed in the supplement. On the second day, the NHEKs were washed twice with PBS(-) (KOHJIN BIO 1620015), and 2 mL of PBS(-) was dispensed per well. In this state, the cells were irradiated with 32 mJ of UVB using DERMARAY 200 (TOSHIBA). Next, the PBS(-) was removed, and a sample in which the test sample was dissolved in Humedia KB2 medium containing insulin, hydrocortisone, and antibiotics among the attached additives was added, and the cells were cultured in an incubator at 37°C and 5% CO2. The final concentration of the test sample in the medium in each well was adjusted to the concentration shown in Figure 1. A control was also prepared in the same manner except that the test sample was not added. The test samples used were sodium hyaluronate (molecular weight 1.2 million), Belamcanda camara extract (Belamcanda camara 1.17%) (rhizome extract), sage extract (Sage 1%) (whole plant, leaf, or flower extract), and Geranium phillyraeoides extract (Geranium phillyraeoides 1%) (whole plant extract). Three samples were cultured for each test sample.

[0069] On the third day, the cell supernatant was collected, and prostaglandin E2 in the culture supernatant was quantified using a PGE2 High Sensitivity ELISA kit (Enzo ADI-931-001) according to the attached manual of the measurement kit. The results are shown in Figure 1. "%" in Figure 1 is the mass % of the extract. Prostaglandin E2 was quantified for each of the three samples, and in the Dunnett test, p<0.05 was marked as *, and p<0.001 was marked as ***. Belamcanda umbellata extract, sage extract, Geranium globulus extract, and sodium hyaluronate significantly reduced the amount of prostaglandin E2. It was found that Belamcanda umbellata extract, sage extract, Geranium globulus extract, and sodium hyaluronate suppress the onset of inflammation caused by UV irradiation by reducing the amount of inflammation-inducing substances generated by UV irradiation. Since the effect was observed especially when added before and after UV irradiation, it is expected that the preparation containing these test samples will be effective by applying it before UV exposure, and even more remarkable effects are expected by reapplying it after UV exposure.

[0070] (2) Evaluation of anti-inflammatory effect (reduction of endothelin-1) On the first day, human normal epidermal keratinocytes (NHEK) (KURABO KK-4009) were cultured in a 6-well plate (Corning 3516 plate) at 4 × 10 5 The cells were seeded at 1000 cells / well and cultured in an incubator at 37°C with 5% CO2. Humedia KG2 medium contains all the additives listed in the supplement. On the second day, the NHEKs were washed twice with PBS(-) (KOHJIN BIO 1620015), and 2 mL of PBS(-) was dispensed per well. In this state, the cells were irradiated with 32 mJ of UVB using DERMARAY 200 (TOSHIBA). Next, the PBS(-) was removed, and a sample in which the test sample was dissolved in Humedia KB2 medium containing insulin, hydrocortisone, and antibiotics among the attached additives was added, and the cells were cultured in an incubator at 37°C and 5% CO2. The final concentration of the test sample in the medium in each well was adjusted to the concentration shown in Figure 2. A control was also prepared in the same manner except that the test sample was not added. The test samples used were Belamcanda chinensis extract (Belamcanda chinensis 1.17%) (rhizome extract), sage extract (Sage 1%) (whole plant, leaf, or flower extract), and bilberry extract (bilberry 0.2%) (leaf extract). Three samples were cultured for each test sample.

[0071] On the fifth day, the cell supernatant was collected, and endothelin-1 in the culture supernatant was quantified using Quantikine ELISA Endothelin1 (R&D System DET100) according to the manual attached to the measurement kit. The results are shown in Figure 2. "%" in Figure 2 is the mass % of the extract. Endothelin-1 was quantified for each of the three samples, and in the Dunnett test, p<0.05 was marked as *, p<0.01 as **, and p<0.001 as ***. Belamcanda chinensis extract, sage extract, and bilberry extract significantly reduced the amount of endothelin-1. It was found that Belamcanda chinensis extract, sage extract, and bilberry extract suppress the onset of inflammation caused by UV irradiation by reducing the amount of inflammation-inducing substances produced by UV irradiation. Since the effects were particularly evident when added before and after UV irradiation, it is expected that the preparations containing these test samples will be effective by applying them before UV exposure, and even more pronounced effects will be expected by reapplying them after UV exposure.

[0072] (3) Evaluation of reactive oxygen scavenging ability On day 1, human normal epidermal keratinocytes (NHEK) (KURABO KK-4009) were cultured in a 96-well plate (96-well Falcon 353219 plate) at 1 × 10 4 The cells were seeded at 1000 cells / well and cultured in an incubator at 37°C with 5% CO2. Humedia KG2 medium was supplemented with all the additives included with this medium. On the third day, samples were prepared by dissolving the test samples in Humedia KB2 medium supplemented with only the antibiotics from the attached additives, and added to the wells containing NHEKs. The final concentration of the test sample in the medium in each well was adjusted to the concentrations shown in Figure 3. A control was also prepared in the same manner, except that the test sample was not added. The test samples used were Belamcanda camara extract (Belamcanda camara 1.17%) (rhizome extract), sage extract (Sage 1%) (whole plant, leaf, or flower extract), Geranium robertii extract (Geranium robertii 1%) (whole plant extract), and bilberry extract (bilberry 0.2%) (leaf extract). Three samples were cultured for each test sample.

[0073] Thirty minutes after adding the samples, the medium in the wells was removed, and the ROS-ID Total ROS Detection Kit (ENZO ENZ-51011) was used to add the reactive oxygen species generating reagent and the test sample to the wells containing NHEK. The medium was Humedia KB2 medium supplemented with only the antibiotics from the attached additives, and the final concentration of the test sample in the medium in each well was adjusted to the concentration shown in Figure 3. Thirty minutes later, the medium in the wells was removed, washed once with PBS(-), and the reactive oxygen scavenging ability was evaluated with PBS(-) added to the wells. For the evaluation, the imaging system ImageXpress Micro (Molecular Devices) was used to obtain cell images stained with fluorescent dyes and measure the brightness. The higher the brightness, the more reactive oxygen there is. The results are shown in Figure 3. "%" in Figure 3 is the mass % of the extract. The active oxygen scavenging ability of each of the three samples was measured, and in the Dunnett test, p<0.05 was marked as * and p<0.001 was marked as ***. The Belgian Bellflower extract, sage extract, Geranium globulus extract, and bilberry extract significantly reduced the amount of active oxygen compared to the control without the addition of any test sample.

[0074] Formulation examples of the topical composition of the present invention are shown below. "%" indicates "% by mass." [Table 1] [Table 2] [Table 3] [Table 4] [Table 5] [Table 6] [Table 7] [Table 8] When the topical compositions shown in Tables 1 to 8 were applied to the skin, spots and pigmentation caused by sunburn could be prevented. [Industrial Applicability]

[0075] The agent of the present invention, which contains Belamcanda chinensis extract, sage extract, Geranium globulus extract, and / or bilberry extract, has the effect of suppressing melanin production by eliminating active oxygen in human skin and suppressing inflammatory reactions caused by ultraviolet light exposure.

Claims

1. The inflammation suppressant contains at least one plant extract selected from the group consisting of Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract.

2. The inflammation suppressant according to claim 1, wherein the inflammation is caused by ultraviolet rays.

3. The inflammation suppressant according to claim 1 or 2, which suppresses inflammation by reducing the amount of inflammation-inducing substances.

4. The inflammation suppressant according to claim 1 or 2, further comprising a mucopolysaccharide.

5. The active oxygen scavenger contains at least one plant extract selected from the group consisting of Belamcanda chinensis extract, sage extract, Geranium globulus extract, and bilberry extract.

6. The active oxygen scavenger according to claim 5, further comprising a mucopolysaccharide.

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