Oral composition

The oral composition combining collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria, with optional ceramide and acerola, addresses the insufficient cosmetic and taste issues of existing formulations by promoting skin cell activation, collagen absorption, and improving taste.

JP2025122592AActive Publication Date: 2025-08-21TOYO SHINYAKU KK
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Patent Information

Application Number
JP2024018209
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Filing Date
2024-02-08
Publication Date
2025-08-21
Estimated Expiration
2044-02-08

AI Technical Summary

Technical Problem

Existing oral compositions containing collagen and barley leaves do not provide sufficient cosmetic and taste-improving effects.

Method used

An oral composition comprising collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria, optionally with ceramide and acerola, to enhance cosmetic effects and improve taste.

Benefits of technology

The composition exhibits excellent cosmetic effects by improving skin cell activation and collagen absorption, while also enhancing taste characteristics such as odor, smoothness, and aftertaste.

✦ Generated by Eureka AI based on patent content.

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Abstract

To provide an oral composition having a cosmetic effect and enhanced taste.SOLUTION: An oral composition includes collagen, young barley leaves, soluble dietary fiber, oligosaccharides, and lactic acid bacteria. Preferably, the oral composition additionally includes at least one selected from ceramide and acerola.SELECTED DRAWING: None
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Description

[Technical Field]

[0001] The present invention relates to an oral composition comprising collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. [Background technology]

[0002] Conventionally, dietary compositions for green juice containing collagen and green leaves of plants such as barley stems and leaves have been known to have excellent beauty and health effects (see, for example, Patent Document 1). It has also been known that combining barley leaf powder with porcine collagen and fish collagen in a specific ratio improves the smell and taste of collagen (see, for example, Patent Document 2). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Japanese Patent Application Laid-Open No. 2006-288350 [Patent Document 2] Japanese Patent Application Laid-Open No. 2009-291128 Summary of the Invention [Problem to be solved by the invention]

[0004] However, these effects are not sufficient, and further improvements are needed.

[0005] An object of the present invention is to provide an oral composition containing collagen, young barley leaves, etc., which has excellent cosmetic effects and taste-improving effects. [Means for solving the problem]

[0006] The present inventors discovered that by combining collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria, excellent beauty effects can be obtained and taste can be improved, leading to the completion of the present invention.

[0007] That is, the present invention is as follows. [1] An oral composition containing collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. [2] The oral composition according to [1] above, further comprising one or more selected from ceramide and acerola. [3] The oral composition according to [1] above, wherein the collagen comprises one or more collagens selected from collagens derived from pigs or fish. [4] The oral composition according to [1] above, wherein the amount of young barley leaves blended is 10% by mass or more. [5] The oral composition according to [1] above, wherein the water-soluble dietary fiber is indigestible dextrin. [6] The oral composition according to [1] above, wherein the oligosaccharide is at least one kind of oligosaccharide selected from those having three or more sugars. [7] The oral composition according to [1] above, wherein the lactic acid bacteria is at least one species of lactic acid bacteria selected from the genera Bifidobacterium and Bacillus. [Effects of the Invention]

[0008] According to the present invention, an oral composition having excellent cosmetic effects can be provided by containing collagen and young barley leaves, etc. Furthermore, according to the present invention, an oral composition having improved taste characteristics such as the odor, smoothness in the throat, and aftertaste that are characteristic of collagen can be provided. DETAILED DESCRIPTION OF THE INVENTION

[0009] The oral composition of the present invention is characterized by containing collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

[0010] The oral composition of the present invention exhibits excellent cosmetic effects. Specifically, the oral composition of the present invention exhibits excellent cosmetic effects by improving the cell activating activity of human skin fibroblasts and promoting collagen absorption.

[0011] Therefore, the oral composition of the present invention can be used as a cosmetic composition, a composition for collagen absorption, etc.

[0012] Furthermore, by combining collagen with young barley leaves or the like, the oral composition of the present invention improves the taste of the collagen-containing oral composition, making it possible to provide an oral composition that is easy to drink.

[0013] Hereinafter, each component contained in the oral composition of the present invention (hereinafter, sometimes referred to as the component of the present invention) will be described. [collagen] Collagen may be animal-derived or synthetic, and includes collagen protein, collagen peptides obtained by hydrolyzing collagen protein, and atelocollagen, which is obtained by treating collagen molecules with protease to remove the telopeptide portion. Collagen derived from pigs or fish is particularly preferred as animal-derived collagen. The average molecular weight (weight-average molecular weight) of collagen is not particularly limited, but is preferably 500 to 100,000, and more preferably 1,000 to 50,000.

[0014] [Young barley leaves] Barley (Hordeum vulgare L.) is an annual or biennial herb belonging to the Poaceae family, believed to be native to Central Asia, and is broadly classified into two-row barley, six-row barley, etc., depending on the panicle shape. The barley leaves used in the oral composition of the present invention are not particularly limited as long as they are commonly available, and any variety of barley leaves, such as two-row barley or six-row barley, may be used. Furthermore, the barley leaves may also include stems.

[0015] In the present invention, for example, pulverized barley leaves, squeezed juice, extracts, etc. can be used. Examples of pulverized barley leaves include dried powder, shredded barley leaves and their dried products (dried shredded barley leaves), granules, etc. The squeezed juice and extract may be in liquid form, but can also be used as a paste or dried powder (juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (warm water, hot water), ethanol, and aqueous ethanol. In the present invention, it is preferable to use dried powder of barley leaves (pulverized barley leaves powder) in order to further enjoy the effects of the present invention.

[0016] [Water-soluble dietary fiber] Water-soluble dietary fiber refers to water-soluble indigestible components (dietary fiber) contained in food that cannot be digested by human digestive enzymes. The water-soluble dietary fiber in the present invention is not particularly limited, and examples thereof include indigestible dextrin, pectin, guar gum, guar gum hydrolysate (enzymatic hydrolysate of guar bean), agarose, glucomannan, polydextrose, alginic acid and its salts, β-glucan, inulin, carrageenan, fucoidan, and derivatives thereof. These can be used alone or in combination of two or more, and it is preferable to use in combination of two or more. In the present invention, it is preferable to use indigestible dextrin in order to further enjoy the effects of the present invention.

[0017] [Oligosaccharides] The term "oligosaccharide" as used herein refers to an oligosaccharide, a compound formed by glycosidic bonds between three to twenty sugars. Specific examples of oligosaccharides used herein include, but are not limited to, indigestible oligosaccharides such as lactoferrin oligosaccharides, fructooligosaccharides, galactooligosaccharides, xylooligosaccharides, and soybean oligosaccharides, and digestible oligosaccharides such as maltooligosaccharides, isomaltooligosaccharides, beet oligosaccharides (raffinose), lactulose, and lactitol. The number of sugars constituting the oligosaccharide is preferably from three to eighteen sugars, more preferably from three to fourteen sugars, even more preferably from three to decasaccharides, and particularly preferably from three to heptasaccharides.

[0018] In the present invention, isomaltooligosaccharides or beet oligosaccharides (raffinose) are preferably used in order to obtain the effects of the present invention more effectively. It is also preferable to combine two or more types of oligosaccharides, and it is more preferable to combine two or more types of digestible oligosaccharides. The physiological functions of indigestible oligosaccharides include being able to reach the large intestine without being degraded by human digestive enzymes, contributing to the activation and proliferation of intestinal bifidobacteria, and improving the intestinal environment, while the physiological functions of digestible oligosaccharides include being able to be degraded by human digestive enzymes, absorbed in the stomach and small intestine, and used as an energy source.

[0019] [Lactic acid bacteria] In the present invention, lactic acid bacteria refer collectively to bacteria that produce lactic acid through metabolism, including bifidobacteria, and examples of lactic acid bacteria include those of the genera Bifidobacterium, Lactobacillus, Enterococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, and Bacillus. Bifidobacterium genus includes Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus genus includes Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus fermentum, Enterococcus faecalis(sometimes referred to as Streptococcus faecalis), Enterococcus faesium (sometimes referred to as Streptococcus faesium), the genus Streptococcus includes Streptococcus thermophilus and Lactococcus lactis (sometimes referred to as Streptococcus lactis), the genus Leuconostoc includes Leuconostoc mesenteroides and Leuconostoc oenos, the genus Pediococcus includes Pediococcus acidilactici and Pediococcus pentosaceus, the genus Staphylococcus includes Staphylococcus carnosus and Staphylococcus xylosus, the genus Tetragenococcus includes Tetragenococcus halophilus, and the genus Bacillus includes Bacillus coagulans and Bacillus mesentericus. These lactic acid bacteria can be used singly or in combination of two or more, preferably in combination of two or more. The method for obtaining the lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt or vegetables, or commercially available products can be used.

[0020] In the present invention, lactic acid bacteria of the genus Bifidobacterium and Bacillus are preferred, and Bifidobacterium longum and Bacillus coagulans (spore-forming lactic acid bacteria) are particularly preferred, as they allow for greater enjoyment of the effects of the present invention.

[0021] [Ceramide] The oral composition of the present invention preferably contains ceramide in addition to the above-mentioned components of the present invention. In order to further enjoy the effects of the present invention, the ceramide may be human ceramides such as ceramide 1, ceramide 2, and ceramide 3, animal-derived ceramides extracted from the brain, spinal cord, etc. of cows, horses, pigs, etc., or plant-derived ceramides extracted from wheat, rice, soybeans, spinach, corn, konjac, pineapple, etc. In addition, the ceramide used in the present invention may be a glycoceramide, and specific examples include those bound to monosaccharides such as galactosylceramide and glucosylceramide, and those bound to oligosaccharides.

[0022] In the composition of the present invention, it is preferable to use a plant-derived ceramide, and it is more preferable to use a glucosylceramide derived from a grass family plant such as wheat, corn, or rice. Among these, it is particularly preferable to use glucosylceramide extracted from rice harvested from rice, as this can achieve a higher effect of the present invention. Examples of solvents used for extraction include water; lower alcohols such as ethanol, methanol, isopropanol, and butanol; lower esters such as ethyl acetate and methyl acetate; acetone; and mixed solvents of these with water. Ethanol or aqueous ethanol is preferred because it allows for efficient extraction of active ingredients. The temperature of the extraction solvent can be appropriately set between room temperature and the boiling point depending on the solvent used.

[0023] [Acerola] The oral composition of the present invention preferably contains acerola in addition to the above-described components of the present invention. Acerola is a tropical fruit tree belonging to the family Malpighiaceae, and its scientific name is Malpighia emarginata. The fruit of acerola is rich in vitamin C and polyphenols. In the present invention, the fruit is preferred as the part of acerola used, as it can better enjoy the effects of the present invention. Furthermore, various processed acerola products can be used in the present invention, and preferred processed forms are pulverized or pulverized powder, squeezed or squeezed powder, and extract or extract powder, with squeezed or squeezed powder being more preferred. In the present invention, it is most preferred to use fruit juice or squeezed powder, as it can better enjoy the effects of the present invention.

[0024] In the oral composition of the present invention, in order to obtain the effects of the present invention more effectively, the amount of collagen blended is preferably 25% by mass or more, more preferably 35% by mass or more, and even more preferably 45 to 65% by mass.

[0025] Furthermore, the amount of young barley leaves in the oral composition of the present invention is preferably 5% by mass or more, more preferably 10% by mass or more, and even more preferably 15 to 50% by mass, in order to better enjoy the effects of the present invention.

[0026] The blending mass ratio of the three components of the present invention other than collagen and young barley leaves is, for example, 1:20 to 0.0001 for water-soluble dietary fiber and oligosaccharide, preferably 1:15 to 0.001, and more preferably 1:10 to 0.01 for water-soluble dietary fiber and lactic acid bacteria, for example, 1:15 to 0.0005, preferably 1:10 to 0.005, and more preferably 1:5 to 0.05 for water-soluble dietary fiber and lactic acid bacteria, and, for example, 1:50 to 0.001, preferably 1:25 to 0.01, and more preferably 1:5 to 0.1 for lactic acid bacteria and oligosaccharide.

[0027] Furthermore, when the oral composition of the present invention contains ceramide, the amount of ceramide to be incorporated is preferably 0.00001% by mass or more, more preferably 0.0001% by mass or more, and even more preferably 0.001 to 1% by mass, in order to better enjoy the effects of the present invention.

[0028] Furthermore, when the oral composition of the present invention contains acerola, the amount of acerola added is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, and even more preferably 0.01 to 1% by mass, in order to better enjoy the effects of the present invention.

[0029] The intake amount of the oral composition of the present invention is not particularly limited, but in order to enjoy the effects of the present invention more effectively, it is preferable that the daily intake amount be 2 g / day or more, more preferably 3 g / day or more, and even more preferably 4 g / day or more. The oral composition of the present invention can be stored as a daily amount in a single container or divided into, for example, 2 to 3 multiple containers so that the daily intake amount is the above-mentioned intake amount.

[0030] As described above, the oral composition of the present invention can be used as a cosmetic composition, a composition for collagen absorption, a composition for promoting collagen absorption, etc., and can be used, for example, as so-called health foods such as functional foods whose efficacy has been approved by a designated organization, such as foods for specified health uses, foods with nutrient functions, and foods with functional claims, or as pharmaceuticals (including quasi-drugs).

[0031] The cosmetic composition of the present invention is not particularly limited as long as it contains the components of the present invention and can be distinguished from other products in that it is used for beauty purposes. For example, the scope of the present invention includes products that display a beauty effect on the product itself, packaging, instructions, or promotional materials (advertising media). In addition, functional food products include those that use beauty effects as the scientific basis for their functionality.

[0032] The cosmetic composition of the present invention is not limited to those that display the component of the present invention as an active ingredient on the product packaging, etc. For example, it may be a product in which the active ingredient is not specified. Furthermore, even general foods that are manufactured and sold with suggested uses are included within the scope of the present invention. For example, foods sold with personal testimonials of those who have consumed the food, mentioning its beauty effects as personal impressions, posted on websites, etc., are also included within the scope of the present invention.

[0033] Specific examples of the cosmetic composition of the present invention include so-called health foods labeled with such slogans as "For those who want to stay beautiful and healthy," "Supports youthful days," "To maintain youthfulness," "For those concerned about moisture," "For those concerned about beauty and health," "For those who want to take in nutrients and beauty ingredients together," and "For those who want to stay beautiful even as they age."

[0034] Examples of the form of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, particles, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, jellies, chewable tablets, and sticks. Among these, tablets, capsules, powders, granules, liquids, and jellies are preferred because they can better enjoy the effects of the present invention. Specific examples include supplements, packaged beverages filled in PET bottles, cans, jars, etc., instant drinks (powdered drinks) that can be dissolved in water (hot water), milk, fruit juice, green juice, etc., and jelly-like foods and beverages. These are preferred because they are easy to drink during meals and can enhance palatability.

[0035] The oral composition of the present invention can be prepared by adding other ingredients than those mentioned above, if necessary, and by a known method such as mixing.

[0036] The oral composition of the present invention is particularly preferably used as a granulated product. Granulated compositions have improved dispersibility in liquids and ease of drinking, making them suitable for use as powdered beverages, and can be used as granulated beverage products. A granulated beverage product refers to a granulated product (a powdered beverage product that is a granulated product) that is mixed with a liquid such as water, hot water, milk, or soy milk by a consumer at the time of ingestion and consumed as a beverage.

[0037] The granulation method is not particularly limited and may be selected from granulation methods commonly used by those skilled in the art. Examples include fluidized bed granulation, extrusion granulation, tumbling granulation, and stirring granulation. Regardless of the granulation method, a granulated product can be produced using any commercially available granulation device by appropriately setting various production conditions such as the drying temperature. From the viewpoint of obtaining a granulated product with excellent ease of granulation and dispersibility, fluidized bed granulation is particularly preferred. Fluidized bed granulation is a method in which raw material powder particles are fluidized by blowing air on them with an air flow, and then a binding liquid such as water is sprayed onto them to bind the powder particles together and form a granulated product, which is an agglomerate of the powder particles. [Example]

[0038] The present invention will be described in more detail below based on examples, but the present invention is not limited to these examples. [Test 1] The cell activation activity of normal human dermal fibroblasts (NHDF) was confirmed using the composition of the present invention. Normal human dermal fibroblasts are widely used as an excellent model system for studying cell physiology, and by confirming their cell activation activity, it is possible to confirm cosmetic effects such as improved skin moisturization by maintaining and improving skin function.

[0039] The test substances in this experiment are as follows: The collagen used was commercially available fish-derived collagen and pig-derived collagen. Specifically, fish scales and skin were washed with water, extracted with warm water, enzymatically decomposed into small molecules, purified, and powdered to produce fish-derived collagen peptides, and pig skin was washed with water, treated with acid, extracted with warm water, and the gelatin was enzymatically decomposed into small molecules, purified, and powdered to produce pig-derived collagen peptides.

[0040] The barley leaves used were dried and crushed powder of young leaves (manufactured by Toyo Shinyaku Co., Ltd.) Specifically, fresh young leaves were cut, blanched, dried, crushed, and passed through a sieve with openings of 1 mm or less.

[0041] As the water-soluble dietary fiber, we used commercially available indigestible dextrin made from tapioca starch, with a dietary fiber content of 38-45% as measured by enzyme-liquid chromatography.

[0042] As oligosaccharides, commercially available beet oligosaccharide (raffinose) and isomaltooligosaccharide were used.

[0043] The lactic acid bacteria used were commercially available Bifidobacterium longum and Bacillus coagulans (spore-forming lactic acid bacteria). Specifically, the Bifidobacterium longum was a dry powder of live bacteria with a bacterial count of 150 billion or more per gram, and the Bacillus coagulans (spore-forming lactic acid bacteria) was a dry bacterial cell with a lactobacillus spore count of 6 billion or more per gram.

[0044] The ceramide used was a commercially available rice-derived ceramide. Specifically, the extract powder containing 10% or more of glucosylceramide was obtained by extracting rice seeds from Oryza sativa Linne (Gramineae) with ethanol. As the acerola, commercially available concentrated acerola juice powder was used. As the dextrin, commercially available powdered dextrin was used.

[0045] The specific experimental procedures are as follows. (1) In a 37°C, 5% CO2 incubator, 75 cm 2 Commercially available normal human dermal fibroblasts were cultured in flasks using 10% FBS-DMEM medium.

[0046] (2) Cells suspended by trypsin treatment were collected at 75 cm 2 1.5 x 10 cells from a flask into a 96-well plate 3 The cells were seeded at a density of 100 cells / well and pre-cultured for 24 hours in a 5% CO2 incubator at 37°C.

[0047] (3) After removing the medium, the test substance was mixed at the ratio shown in Table 1 below, and 100 μL / well of the medium containing the test substance was added, followed by culturing for 24 hours in a 37°C, 5% CO2 incubator.

[0048] (4) After 24 hours of culture, the medium was removed and the cells were washed with PBS. (5) 100 μL / well of a 500 μg / mL MTT (Nacalai Tesque) solution prepared with serum-free DMEM was added to each well, and the plate was left to stand in a 37°C, 5% CO2 incubator to adequately stain the cells. (6) After incubation, the MTT solution was removed and the cells were washed with PBS.

[0049] (7) 100 μL / well of isopropanol was added, and after confirming that the dye had been eluted out of the cells, the absorbance at 570 nm and 650 nm was measured.

[0050] (8) Based on the obtained data, the % of control (the relative number of viable cells, with the number of viable cells in the control being 100%) was calculated, and the results of evaluating the cell activation activity for each Example and Comparative Example are shown in Table 1 as relative values ​​when the value for Comparative Example 1 is set to 100.

[0051] % of control = (Data sample - Data blank) / (Data control - Data blank) × 100

[0052] [Table 1]

[0053] As shown in Table 1, the compositions of the Examples containing the five components of the present invention exhibited synergistically improved cell-activating activity compared to the Comparative Examples, which did not contain some of the components of the present invention. Specifically, when comparing Comparative Example 1, which contained two components (water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides) with Examples 1 and 2, which contained five components (collagen and young barley leaves) in addition to these, the cell-activating activity was improved in Examples 1 and 2. Similarly, when comparing the results of Examples 1 and 2 with those of Example 3, the cell-activating activity was further improved by adding two types of collagen. Similarly, when comparing the results of Examples 3 and 4, the cell-activating activity was further improved by adding two types of lactic acid bacteria and two types of oligosaccharides. Furthermore, when comparing the results of Examples 3, 5, and 6, the cell-activating activity was further improved by adding ceramide or acerola. Furthermore, when comparing the results of Examples 4 to 6 with those of Example 7, the cell-activating activity was particularly improved by adding ceramide and acerola. The composition of the present invention improved the cell activation activity of normal human skin fibroblasts, an excellent model system for studying cell physiology, and was therefore confirmed to exhibit cosmetic effects such as improving skin moisturizing by maintaining and improving skin function.

[0054] [Test 2] The composition of the present invention was used to confirm the collagen absorption-promoting effect of the composition on the monolayer permeability of a human colon cancer-derived cell line. Monolayer permeability of a human colon cancer-derived cell line is a common in vitro model used to evaluate the absorption of compounds through the human intestine. By confirming collagen permeability, cosmetic effects such as improved skin firmness and elasticity due to collagen absorption promotion can be confirmed.

[0055] The test substances used in this experiment were the same as those used in Test 1.

[0056] The specific experimental procedures are as follows. (1) In a 37°C, 5% CO2 incubator, 75 cm 2 A commercially available human colon cancer cell line was cultured in a flask using 10% FBS-1% NEAA-DMEM medium.

[0057] (2) Trypsinized cells were suspended and placed in insert wells of a 24-well plate at 4 × 10 4 The cells were seeded at a density of 100 cells / well and pre-cultured for 24 hours in a 5% CO2 incubator at 37°C. (3) After 24 hours, the medium was changed, and the cells were then cultured for 21 days, with the medium changed every 3 to 4 days, to form a monolayer. (4) After the monolayer was formed, the electrical resistance was measured and the plate was washed with HBSS buffer.

[0058] (5) Test substances were mixed at the ratios shown in Table 2 below, and 50 μL / well of medium containing the test substances was added. The cells were cultured for 2 hours in a 5% CO2 incubator at 37°C. HBSS buffer was used as the medium, and the total concentration of the test substances in the medium was 1000 μg / mL.

[0059] (6) The amount of collagen in the permeate was measured using a commercially available kit, and the amount of collagen absorption was shown in Table 2 as a relative value with the value for Comparative Example 2 taken as 100.

[0060] [Table 2]

[0061] As shown in Table 2, the compositions of the Examples containing the five components of the present invention synergistically improved collagen absorption compared to the Comparative Examples, which did not contain some of the components of the present invention. Specifically, when comparing Comparative Example 2, which contained two components (water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides) with Examples 8 and 9, which contained five components (collagen and young barley leaves) in addition to these, collagen absorption was improved in Examples 8 and 9. Similarly, when comparing the results of Examples 8 and 9 with those of Example 10, the addition of two types of collagen further improved collagen absorption. Similarly, when comparing the results of Examples 10 and 11, the addition of two types of lactic acid bacteria and two types of oligosaccharides further improved collagen absorption. Furthermore, when comparing the results of Examples 10 with those of Examples 12 and 13, the addition of ceramide or acerola further improved collagen absorption. Furthermore, when comparing the results of Examples 11 to 13 with those of Example 14, the addition of ceramide and acerola improved collagen absorption in particular. The composition of the present invention improved the collagen monolayer permeability in a human colon cancer-derived cell line, a common in vitro model used to evaluate the absorbability of compounds through the human intestine, and was therefore confirmed to exhibit cosmetic effects such as improving skin firmness and elasticity by promoting collagen absorption.

[0062] [Test 3] The taste-improving effect of the composition of the present invention was confirmed. The test substances used in this experiment were the same as those used in Test 1.

[0063] Each sample was obtained by mixing 5 g of powder sample with 150 ml of water according to the blending ratios shown in Table 3. Of these samples, the sample obtained from the powder sample of Comparative Example 2 was used as the standard. The sensory evaluation was conducted by panelists who regularly consume green juice and have experience in sensory evaluation. The panelists were asked to evaluate the collagen-specific odor, throat feel, texture, and aftertaste.

[0064] <Collagen-specific odor> ◎: No odor and tastes much better than the standard product. ○: It has less odor and tastes better than the standard product. △: Similar to the standard product. ×: Below standard.

[0065] <Smoothness> ◎: It goes down smoothly and feels much easier to drink than the standard product. ○: It goes down smoothly and feels easier to drink than the standard product. △: Similar to the standard product. ×: Below standard.

[0066] <Texture> ◎: It feels smooth on the tongue and is much easier to drink than the standard product. Good: Feels smooth on the tongue and is easier to drink than the standard product. △: Similar to the standard product. ×: Below standard.

[0067] <Aftertaste> ◎: The aftertaste is good and the mouth feels much cleaner than the standard product. ○: The texture is good and the inside feels cleaner than the standard product. △: Similar to the standard product. ×: Below standard.

[0068] "comprehensive evaluation" The overall evaluation was based on the evaluation of the collagen-specific smell, throat feel, texture, and aftertaste, and was determined according to the following criteria. The evaluation results are shown in Table 3 above. ◎: Excellent, with neither × nor △. ◯: No X or △, good. △: There are △ marks but no × marks, so it is acceptable. ×: There is one or more ×s, and it is not possible.

[0069] [Table 3]

[0070] As shown in Table 3, the compositions of the examples containing the five components of the present invention had improved taste compared to the comparative compositions not containing some of the components of the present invention. Specifically, when comparing Comparative Example 3, which contained two types of collagen but no other components, with Comparative Example 4, which also contained young barley leaves, the collagen odor was reduced in Comparative Example 4, but other evaluation items such as smoothness were comparable to Comparative Example 3. On the other hand, Example 15, which contained the five components of the present invention, was superior in all evaluation items compared to the results of Comparative Examples 3 and 4. That is, while the taste-improving effect of the two components, collagen and young barley leaves, was insufficient (Comparative Examples 3 and 4), the taste was improved in all evaluation items by incorporating water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides into the five components. Furthermore, comparing the results of Examples 15 and 16, it was found that by using two types of lactic acid bacteria and oligosaccharides and adding ceramide and acerola, the taste was further improved, resulting in a composition that was easy to drink.

[0071] [Production Examples 1 to 3] (Production of powdered beverages) As shown in Table 4, collagen was mixed with other ingredients, the composition was granulated, and a powdered beverage was produced. When 5 g of the resulting powdered beverage was mixed with 150 mL of water and ingested, it was found to be easy to drink and had excellent effects on maintaining and / or improving skin moisture and firmness.

[0072] [Table 4] [Industrial Applicability]

[0073] The oral composition of the present invention can be used as a health food or the like, and is therefore highly useful industrially.

Claims

1. An oral composition containing collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

2. The oral composition according to claim 1, further comprising one or more selected from ceramide and acerola.

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