Rapid detection method of acid orange II by time-resolved immunochromatographic test strips

Abstract

The invention discloses a rapid detection method of acid orange II by time-resolved immunochromatographic test strip, which relates to the technical field of food safety detection and comprises the following detection steps: step 1, coupling time-resolved fluorescent microspheres with acid orange II antibodies, and the method has the advantages that compared with the traditional methods of measuring by using large instruments such as high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS / MS) and the like, Time-resolved fluorescent probe was made by coupling time-resolved fluorescent microspheres with acid orange II antibody, and then it was mixed with the sample to be detected, and then it was quickly detected by immunochromatographic test strip. It can be easily detected in laboratory environments, supermarkets, production workshops and other fields, especially suitable for screening a large number of samples, which greatly improved the efficiency and convenience of detection and had a good prospect of popularization and application.