Lactobacillus plantarum NXU0011 and application thereof

Abstract

The present invention belongs to the technical field of microorganisms, and particularly relates to a Lactobacillus plantarum NXU0011 and application thereof. The Lactobacillus plantarum NXU0011 is preserved in the China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO: 26970. The bacterium has a good inhibition effect on Escherichia coli and Staphylococcus aureus. The Lactobacillus plantarum NXU0011 also has good antioxidant performance, and can be used for preparing hypoglycaemic drugs. At the same time, the Lactobacillus plantarum NXU0011 has a high survival rate in saliva, gastric juice or intestinal juice, and has the potential to become a good probiotic effect regulator.

Description

Technical Field The present invention belongs to the technical field of microorganisms, and particularly relates to a . Background Probiotics are a class of active microorganisms beneficial to a host by colonizing in a human body and changing a composition of a flora in a certain site of the host. By regulating mucosal and systematic immune functions of the host or by regulating the balance of an intestinal flora, the probiotics have the effect of promoting absorption of nutrients to maintain intestinal health, thereby producing single microorganisms or mixed microorganisms with a clear composition that are beneficial to health. Beneficial bacteria or fungi in the human body or an animal body mainly include a yeast, probiotic Bacillus, Clostridium butyricum, Lactobacillus, Bidobacterium, Actinomycetes, etc. At present, it has been found through researches that the yeast has the probiotic characteristics of regulating intestinal balance, promoting feed conversion, improving the bodys immune function and the like. The yeast is classified into Saccharomyces, Torulaspora delbrueckii, Candida, l / l / ickerhamomyces, Pichia, Saccharomyces boulardii, Torulopsis candida, Saccharomyces chevalieri, Rhodotorula rubra, Schizosaccharomyces pombe, Saccharomyces Boulard / i, etc. The yeast can be used as a probiotic in food fermentation, can also be used as a medicine, or can be added to a feed to promote growth and development of an animal, shorten a feeding period, increase amounts of meat and eggs, improve the quality of the meat with an increase in a lean meat rate, improve gloss of fur, enhance disease resistance of a young animal and the like. The probiotic Bacillus is a class of aerobic or facultative anaerobic, and spore producing rod-shaped bacteria having the probiotic properties to the body, and is Gram-positive; and Bacillus is widely applied in the industry, the agriculture and pharmaceutical production. The Clostridium butyricum is anaerobic gram-positive Bacillus isolated from an intestinal tract of a healthy human or animal. It has been found through researches that the Clostridium butyricum has good probiotic properties of maintaining the balance of the intestinal flora, enhancing the bodys immune function, producing nutrients, preventing enteritis, etc. The Bidobacterium is a class of physiological bacteria in the human body. It has many important physiological functions of serving as a biological barrier, providing nutrition, resisting a tumour, enhancing immune, improving a gastrointestinal function and resisting aging. Lactic acid bacteria refer to a class of spore-free, gram-positive bacteria with lactic acid as a main product of fermented sugars. The lactic acid bacteria are the most common probiotics, which have been widely applied and considered safe for the human or the animal. The lactic acid bacteria can promote animal growth, regulate the normal flora of the gastrointestinal tract, and maintain the microecological balance, thereby improving the gastrointestinal function, increasing a food digestibility and a biological potency, lowering serum cholesterol, controlling endotoxin, inhibiting growth of spoilage bacteria in an intestine, improving bodys immunity and the like. Diabetes is a metabolic disease characterized by hyperglycaemia. The hyperglycaemia is caused by insulin secretion defects or impaired biological effects of insulin, or both. Long-standing hyperglycaemia leads to chronic damages and dysfunctions of various tissues, especially eyes, kidneys, hearts, blood vessels and nerves. The lactic acid bacteria are widely applied to fermented foods due to probiotic effects and safety of the lactic acid bacteria. The potential function of preventing and treating the diabetes of the lactic acid bacteria has also been widely recognized, and the lactic acid bacteria have been used as a safe and effective biological effect regulator for lowering blood glucose. There are numerous existing lactic acid bacteria with the blood glucose lowering function, having screening sources widely involved in traditional lactic acid bacteria fermentation products in various regions. However, most lactic acid bacteria have DPPH scavenging rates of about 82.92%, or small survival rates in an artificial intestinal fluid, thereby having non-ideal tolerance to a simulated artificial gastrointestinal fluid. Therefore, it is very necessary to research the Lactobaci / lus with better effect. Summary In view of the above technical problems, the present invention provides a Lactobaci / lus plantarum NXU0011, having a DPPH free radical scavenging rate up to 95.17%, and survival rates in simulated saliva, gastric juice and intestinal juice of 119.81%, 39.27% and 122.52% respectively. In a first aspect of the present invention, Lactobaci / lus plantarum NXU0011 is provided, with a preservation number ofCGMCC NO: 26970. In a second aspect of the present invention, a fermentation method for the Lactobaci / lus plantarum NXU0011 is provided, including the following step: inoculating the Lactobaci / lus plantarum NXU0011 to an MRS liquid medium for culture at 37 i 5°C for 24 - 48 h to obtain a Lactobacillus plantarum NXU0011 fermentation broth. In a third aspect of the present invention, a fermentation broth obtained by fermentation according to the above method is provided. In a fourth aspect of the present invention, application of the Lactobacil / us plantarum NXU0011 or the fermentation broth in preparation of a hypoglycaemic product is provided. Preferably, the Lactobacillus plantarum NXU0011 or the fermentation broth is used for preparing an d-amylase inhibitor; The Lactobacillus plantarum NXU0011 or the fermentation broth is used for preparing an d- glucosidase inhibitor. In a fifth aspect of the present invention, application of the Lactobaci / lusplantarum NXU0011 or the fermentation broth in preparation of an antioxidant product is provided. In a sixth aspect ofthe present invention, application ofthe Lactobacil / usplantarum NXU0011 or the fermentation broth in preparation of a bacterium inhibition product is provided. Preferably, bacterium inhibition is to inhibitone ortwo of Escherichia coliand Staphylococcus aureus. Compared with the prior art, the present invention has the beneficial effects: The present invention provides a Lactobacil / usplantarum strain NXU0011. The Lactobacil / us plantarum NXU0011 has good bacterium inhibition performance against the Escherichia coli and the Staphylococcus aureus, with bacterium inhibition diameters of 16.83 mm and 16.28 mm respectively. The Lactobacil / us plantarum NXU0011 has a DPPH free radical scavenging rate up to 95.17%, thereby having good antioxidant performance. The Lactobacillus plantarum NXU0011 has survival rates in simulated saliva, gastric juice and intestinal juice of 119.81%, 39.27% and 122.52% respectively. The Lactobaci / lus plantarum NXU0011 has an d-amylase inhibition rate of 85.35%, and an d-glucosidase inhibition rate of 51.74%, thereby still having an obvious effect on lowering blood sugar on the whole. Biomaterial preservation information: Lactobacillus plantarum strain NXU0011, classified as Lactobaci / lus plantarum, Latin name: Lactobacillus plantarum. The Lactobacillus plantarum NXU0011 was preserved with the preservation number of CGMCC NO: 26970 in the China General Microbiological Culture Collection Center on March 31, 2023, with the address of No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. Description of Drawings Fig. 1 is a diagram showing a colony morphology of Lactobaci / lus plantarum NXU0011 on an MRS medium; Fig. 2 is a diagram showing a bacterium morphology of Lactobaci / lus plantarum NXU0011 under an optical microscope; Fig. 3 is a diagram showing homology comparison results of Lactobaci / lus plantarum NXU0011 in NCBI; Fig. 4 is a growth curve of Lactobacil / us plantarum NXU0011; Fig. 5 is a diagram showing a bacterium inhibition effect of Lactobaci / lus plantarum NXU0011 on Escherichia coli K12, wherein in the figure, different numbers indicate three groups of parallel tests; Fig. 6 is a diagram showing a bacterium inhibition effect of Lactobaci / lus plantarum NXU0011 on Staphylococcus aureusATCC6538, wherein in the figure, 1, 2 and 3 indicate three groups of parallel tests. Detailed Description The present invention will be further described in detail below in conjunction with embodiments and corresponding accompanying drawings. The following embodiments are used for describing the present invention merely rather than limiting the scope of the present invention. Embodiment 1 Lactobacil / us Plantarum NXU0011 Isolation and Screening 1. Sample dilution 25 g of a soil sample around Seshan Jinhe Milk Industry in Helan County, Yinchuan City, Ningxia Province are taken and homogenized in 225mL of sterilewaterto obtain 10'1 soil bacterial suspension. 1 mL of the soil bacterial suspension is fully and uniformly mixed in 9 mL of sterile waterto obtain a 10'2 gradient diluent, and to obtain the other 103, 104, 105, 106, and 10'7 gradient diluents by analogy for later use. 2. Pouring culture 1 mL of 105, 106, and 10'7 gradient soil bacterial suspensions are poured into various plates respectively, with three groups of parallels set for each gradient. Calcium carbonate with a mass fraction of5% is added to an MRS medium sterilized by high-pressure steam as an indicator for being molten by heating, a resultant is cooled to about 45°C, and about 20 mL of medium is poured on each plate. After completion of the operation, the plates are inverted in a 37°C constant temperature incubator for culture for 48 h. 3. Colony observation Plates with uniform colony distribution are selected for observation. Colonies growing well on surfaces or inside of the media, having transparent rings, neatedges and white lusters, and being easily picked up without adhering to the media are selected. 4. Streaking Strains are picked by an inoculation ring, and isolated on the plates by streaking. After completion of the operation, the plates are inverted in a 37°C constant temperature incubator for culture for 48 h. 5. Liquid culture Colonies are picked for enrichment culture in an MRS liquid medium. The colonies are cultured at a constant temperature of 37°C for 48 h to obtain an enriched bacterial suspension. 6. Examination with optical microscope A ring of the enriched bacterial suspension cultured in liquid is picked by the inoculation ring, put on a carrier glass for making a slide, and subjected to Gram staining; and then a morphology of bacteria is observed under a 100-fold oil immersion lens. For the strain NXU0011, an optimum growth temperature is 35-37°C, and an optimum pH value is about 6.5. The colony biological characteristics are as follows: the colony is round, smooth, wet and convex, has a neat edge, and is milky white, as shown in Fig. 1. The bacteria are anaerobic or facultative anaerobic, are Gram-positive bacteria after being observed after Gram slide making and staining, have no spore flagellum, have a morphology of straight Bacillus brevis, with two rounded ends, and are arranged in single, pair, or short chain, as shown in Fig. 2. Embodiment 2 Determination on Lactobacil / us Plantarum NXU0011 A bacterial genomic DNA extraction kit is used to extract genes from different lactic acid bacteria, and universal primers for 16S rDNA of the lactic acid bacteria are used for PCR amplification. Sequences of PCR amplification primers are FA-27F: 5GCAGAGTTCTCGGAGTCACGAAGAGTTTGATCCTGGCTC AG-3 (shown as SEQ ID NO: 1), and RA-1495R:5AGCGGATCACTTCACAC AGGACTACGGGTACCTTGTTACGA-3 (shown as SEQ ID NO: 2). With genomic DNA of the extracted strain as an amplification template, a PCR amplification system is as follows: 5 ul of 10 x PCR buffer, 4 ul of dNTP, 3 ul of primer (F + R), 0.5 ul of a Taq DNA enzyme, 1.5 ul ofa genomic DNA template, and 6 ul of double distilled water. Amplification set conditions are as follows: pre-degeneration for 5 min at 94°C, degeneration for 1 min at 94°C, annealing for 1 min at 58°C, and extension for 2 min at 72°C, with 30 cycles; and end extension for 10 min at 72°C, and heat preservation at 4°C. PCR products are spotted in 1% agarose gel for gel running. 2 ul of a PCR product is mixed with 6 x Loading Buffer; and then a mixture is added to a spotting hole for electrophoresis gel running, and observed by a gel imager. A clear band is observed at 1500 bp, and no obvious non-specific amplification is observed. Through sequencing of the amplification products of 16S rDNA, shown as SEQ ID NO: 3, the sequence is as follows: GGGTGTGACTATAGATGCAGTCGAACGAACTCTGGTATTGATTGGTGCTTGCATCATG ATTTACATTTGAGTGAGTGGCGAACTGGTGAGTAACACGTGGGAAACCTGCCCAGAAGCG GGGGATAACACCTGGAAACAGATGCTAATACCGCATAACAACTTGGACCGCATGGTCCGA GCTTGAAAGATGGCTTCGGCTATCACTTTTGGATGGTCCCGCGGCGTATTAGCTAGATGGT GGGGTAACGGCTCACCATGGCAATGATACGTAGCCGACCTGAGAGGGCAATCGGCCACAT TGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATG GACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAACTC TGTTGTTAAAGAAGAACATATCTGAGAGTAACTGTTCAGGTATTGACGGTATTTAACCAGAA AGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG GATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGC TCAACCGAAGAAGTGCATCGGAAACTGGGAAACTTGAGTGCAGAAGAGGACAGTGGAACT CCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGT CTGGTCTGTAACTGACGCTGAGGCTCGAAAGTATGGGTAGCAAACAGGATTAGATACCCT GGTAGTCCATACCGTAAACGATGAATGCTAAGTGTTGGAGGGTTTCCGCCCTTCAGTGCTG CAGCTAACGCATTAAGCATTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGA ATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAA CCTTACCAGGTCTTGACATACTATGCAAATCTAAGAGATTAGACGTTCCCTTCGGGGACAT GGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCG CAACGAGCGCAACCCTTATTATCAGTTGCCAGCATTAAGTTGGGCACTCTGGTGAGACTGC CGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGG GCTACACACGTGCTACAATGGATGGTACAACGAGTTGCGAACTCGCGAGAGTAAGCTAAT CTCTTAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATC GCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGC CCGTCACACCATGAGAGTTTGTAACACCCAAAGTCGGTGGGGTAACCTTTTAGGAACCAGC CGCCTAAGGTGAGCAGA By entering the NCBI website, it is shown from results of BLAST sequence alignment that the homology in16S rDNA between the strain and Lactobaci / lus plantarum reaches 99.72%; and the strain can be identified as the Lactobacillus plantarum, named Lactobacillus plantarum NXU0011, as shown in Fig. 3. Embodiment 3 Determination on Growth Curve of Lactobacil / us Plantarum NXU0011 The Lactobacil / us plantarum NXU0011 strain is inoculated to the MRS liquid medium with AN inoculation amount of2.0%, and placed in a constanttemperature incubator at37°C for culture for 24 h. A fermentation broth is taken every 2 h; and with sterile MRS liquid medium as a control group, growth of the strain in the fermentation broth is measured by an ultraviolet spectrophotometer. 3 replicates are run atOD600 nm, and absorbance values of the bacteria are recorded to obtain a growth curve, as shown in Fig. 4. A growth curve shows that the Lactobacillus plantarum NXU0011 is in a lag phase on 0-8 h, with an OD value small changed, is in a logarithmic growth phase on 8-12 h, with the OD value increased exponentially, and enters a stable phase on 12-24 h, with the OD value increased slowly, and then showing a certain stable trend. Embodiment 4 Probiotic Function Test on Lactobaci / lus Plantarum NXU0011 1. Determination of digestive stress ability: The bacteria are centrifuged at 8000 x g for 10 min, and washed with sterile PBS (with a pH value of 7.2); and bacterium precipitates are suspended in simulated saliva for culture at 37°C for 5 min. A resultant is centrifuged at 8000 x g for 10 min; and bacterium precipitates are resuspended in a simulated gastric juice for culture at 37°C for 3 h. A resultant is centrifuged at 8000 x g for 10 min; and bacterium precipitates are resuspended in a simulated intestinal fluid for culture at 37°C for 2 h. After each stress, a viable count of Lactobacil / us is measured by a plate colony counting method. Three parallels are set up for each group; and bacteria are cultured at 37°C for 48 h. Survival rates are calculated according to the following formulas: Viable count after bacteria are inoculated to simulated saliva for . treatment for 5 min . . y100% Survival rate / % : V|able count after bactena are inocuiateoi to Simulated saliva for treatment for 0 mln Viable count after bacteria are inoculated to a simulated gastric juice for treatment for 3 h ><100% s rate / % = 22331Tirifiäiiäaiiäii'fiifäf Viable count after bacteria are inoculated to a simulated intestinal juice for treatment for 2 h ><100%) Survival rate / % = Viable count after bacteria areinoculated toasimulated Intestinal jUIce for treatment for 0 h Results show that the survival rate ofthe Lactobacil / usplantarum NXU0011 reaches 119.81% after the Lactobacil / us plantarum NXU0011 is cultured in the simulated saliva at 37°C for 5 min; the survival rate is 39.27% after the Lactobaci / lus plantarum NXU0011 is cultured in the simulated gastric juice at 37°C for 3 h; and the survival rate reaches 122.52% after the Lactobacil / us plantarum NXU0011 is cultured in the simulated intestinal fluid at 37°C for 2 h. 2. Determination on DPPH free radical scavenging ability 2 mL of a DPPH anhydrous ethanol solution, 0.2 mL of a bacterial solution and 1.8 mL of anhydrous ethanol solution are uniformly mixed together, then reacted in the dark for 30 min, and centrifuged at 8000 r / min for 10 min; and a supernatant is taken, and an absorbance value of the supernatant at a wavelength of 517 nm is measured and denoted as A1. Anhydrous ethanol is used to replace the measured sample solution as a control group, and an absorbance value is denoted as Ao. The anhydrous ethanol is used to replace the DPPH anhydrous ethanol solution as a blank control group, and an absorbance value is denoted as A2. 3 replicates are run for each group of samples to take an average. A calculation formula for determining the DPPH free radical scavenging rate ability is as follows: [1-%]x100% DPPH free radical scavenging rate = ° As a result, the DPPH free radical scavenging rate is measured as 95.17%. 3. Determination on bacterium inhibition ability 2 common pathogens are selected: Escherichia coliand Staphylococcus aureus are used as indicator bacteria. Concentrations of an Escherichia coli bacterial suspension and a Staphylococcus aureus bacterial suspension are adjusted to 108-109 CFU / mL. In vitro antibacterial effects of the strain fermentation broth on the Escherichia coli and the Staphylococcus aureus are determined by an Oxford cup method. l mL of the Escherichia coli bacterial suspension, l mL of the Staphylococcus aureus suspension, and 15 mL of the MRS medium that is maintained at about 45°C after being molten are rapidly and uniformly mixed, and then poured onto the plate for full cooling and solidification; and a resultant is put into a plurality of sterilized Oxford cups, and the sterilized Oxford cups are arranged regularly in a certain order. 180 ul of the fermentation broth is added to the Oxford cups for culture at 37°C for 18 h; then a diameter of a bacteriostatic ring is measured by a vernier calliper; and 3 repetitions are set for each probiotic strain, and an average value of the diameters of the bacteriostatic ring is taken as a final diameter of the bacteriostatic ring. According to the judgement criteria for a drug sensitivity test of National Committee for Clinical Laboratory Standards, the diameter of the bacteriostatic ring larger than 15 mm is identified as that the probiotic strain has strong bacterium inhibition ability, and can be used for screening subsequent strains. It is measured that the diameter of the bacteriostatic ring of the Lactobacil / us plantarum NXU0011 against the Escherichia coli is 16.83 mm, and the diameter of the bacteriostatic ring against the Staphylococcus aureus is 16.28 mm, as shown in Fig. 5 (in the figure, three parallel tests are shown), and Fig. 6 (in the figure, three parallel tests are shown). 4. Measurement on d-amylase inhibition rate 0.25 mL of the Lactobacil / us plantarum NXU0011 and 1 mg / mL d-amylase solution are mixed in equal volume for incubation at 37°C for 10 min; then a reaction solution is added to 0.5 mL of a 37°C soluble starch solution with a mass fraction of 1.5% for a reaction for 37°C for 5 min; then 1 mL of a DNS solution is added for a reaction in a boiling water bath for 5 min; then a resultant is rapidly cooled to a room temperature, diluted 10 times and left for standing for 30 min; and an absorbance value is measured at540 nm. A PBS solution (0.1 mol / L, pH = 6.8) is used as a blank control, and 3 parallels are set for each group. (1) ><100% d-amylase inhibition rate (%) = CD In the formula: A is a sample group, containing the sample solution and the d-amylase solution; B is a sample blank group, containing the sample solution, and not containing the d-amylase solution; C is a control group, not containing the sample solution, and containing the d-amylase solution; D is a blank control group, not containing the sample solution and the d-amylase solution. After the Lactobacil / us plantarum NXU0011 is interacted with d-amylase, the catalytic efficiency of the Lactobacil / us plantarum NXU0011 is weakened greatly, and the inhibition rate reaches 85.35%. 5. Measurement on d-glucosidase inhibition rate 30 ul ofa bacterial solution, 50 ul of a PBS solution (0.1 mol / L, pH = 6.8) and 50 ul of a PNPG solution with a concentration of 2.5 mmol / L are added to a 210 ul of a reaction system of a 96- well plate for incubation at 37°C for 10 min. Then 30 ul of an d-glucosidase solution with a concentration of 0.4 U / mL is added for a continued reaction at 37°C for 30 min; and finally, 50 ul of a Na2C03 solution with a concentration of 1 mol / L is added to terminate the reaction. After completion of the reaction, an absorbance value is measured at 405 nm. A PBS solution (0.1 mol / L, pH = 6.8) is used as a blank control, and 3 parallels are set for each group. (l)><100% d-glucosidase inhibition rate (%) = CD In the formula: A is a sample group, containing the sample solution and the d-glucosidase solution; B is a sample blank group, containing the sample solution, and not containing the d- glucosidase solution; C is a control group, not containing the sample solution, and containing the d-glucosidase solution; D is a blank control group, not containing the sample solution and the d-glucosidase solution. In the test, with inhibition of acarbose as a positive control test, the inhibition rate is 62.34%; and the inhibition rate of the Lactobacil / us plantarum NXU0011 is 51.74%. Test methods used in the embodiments are all conventional methods, unless otherwise noted. The materials, reagents, etc. used in the embodiments can all be obtained from a commercial approach, unless otherwise noted. It should be understood that those ordinary skilled in the art can make improvements or transformations according to the above illustration, and all these improvements and transformations shall fall within the protection scope of the appended claims of the present invenon. 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Claims

1. Lactobacillus plantarum NXU0011, with the preservation number CGMCC NO: 26970, deposited on March 31, 2023 at the China General Microbiological Culture Collection Center with the address No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing.

2. A method for fermenting Lactobacillus plantarum NXU0011 according to claim 1, which method comprises the step of inoculating the Lactobacillus plantarum NXU0011 in liquid MRS medium to culture for 24-48 hours at 37 i 5°C to obtain a Lactobacillus plantarum NXU0011 fermentation broth.

3. A fermentation broth obtained by the method according to claim 2.

4. An application of the Lactobacillus plantarum NXU0011 according to claim 1 or the fermentation broth according to claim 3 in the preparation of a product with hypoglycemic effect.

5. The use according to claim 4, wherein the Lactobacillus plantarum NXU0011 or the fermentation broth is used for the preparation of a d-amylase inhibitor.

6. The use according to claim 4, wherein the Lactobacillus plantarum NXU0011 or the fermentation broth is used for the preparation of a d-glucosidase inhibitor.

7. An application of the Lactobacillus plantarum NXU0011 according to claim 1 or the fermentation broth according to claim 3 in the preparation of a product with antioxidant effect.

8. An application of Lactobacillus plantarum NXU0011 according to claim 1 or the fermentation broth according to claim 3 in the preparation of a product with antibacterial effect.

9. The use according to claim 8, wherein the bacterial inhibition comprises inhibiting one or two of the bacteria Escherichia coli and Staphylococcus aureus. Fig. 1 Fig. 2