Antioxidative and Anti-inflammatory composition comprising extract of carrot leaf lava seawater as active ingredient

Abstract

The present invention relates to an antioxidant and anti-inflammatory composition comprising a carrot leaf lava seawater extract as an active ingredient. The carrot leaf lava seawater extract of the present invention has an increased total polyphenol, rutin, and caffeic acid content compared to a carrot leaf water extract, has excellent DPPH radical scavenging activity, and has a remarkable effect of inhibiting NO production. Therefore, the carrot leaf lava seawater extract of the present invention can be useful as an antioxidant and anti-inflammatory composition.

Description

Antioxidant and anti-inflammatory composition containing carrot leaf lava seawater extract as an active ingredient

[0001] The present invention relates to an antioxidant and anti-inflammatory composition containing carrot leaf lava seawater extract as an active ingredient.

[0002] Carrots are a good source of minerals like calcium, phosphorus, iron, and magnesium, as well as a root vegetable rich in various bioactive compounds like carotenoids and dietary fiber. Due to their diverse nutritional benefits, carrot consumption is steadily increasing worldwide. However, the aerial parts of carrots are still not widely consumed. Therefore, if functional research on the aerial parts of carrots is conducted to develop high-value-added products, they could serve as a new source of income for farmers.

[0003] Meanwhile, lava seawater is groundwater that has been naturally filtered by volcanic bedrock. In Jeju Island, lava seawater has been used as breeding water for flounder farms since the 1980s, taking advantage of its low temperature and cleanliness characteristics. Its industrial value is highly evaluated and is receiving much attention in areas such as beauty, food, and spas.

[0004] The lava seawater distributed in the eastern region of Jeju Island (Gujwa-eup, Seongsan-eup, and Pyoseon-myeon) is characterized by its clean water and consistent temperature year-round. It also contains abundant beneficial minerals, including essential minerals like sodium, calcium, and magnesium, as well as rare minerals like vanadium, germanium, and selenium not found in regular groundwater or seawater. While active research has focused on the stability and various physiological activities of lava seawater itself, little research has been conducted on the production of extracts utilizing lava seawater.

[0005] Lava seawater has similar mineral composition to deep seawater, but it is economical because it is cheaper to extract than deep seawater. Therefore, high value-added products can be developed through research on the various functions of extracts using lava seawater.

[0006] Meanwhile, Korean Patent Publication No. 2023-0081972 discloses a hair loss prevention / hair growth promotion composition containing lava seawater and natural extracts and a method for producing the same, and Korean Registration Patent No. 2104861 discloses a method for producing lava seawater-aged zelkova leaf powder and a composition for improving skin wrinkles using an extract of the powder. However, no disclosure has been made regarding an antioxidant and anti-inflammatory composition containing the carrot leaf lava seawater extract of the present invention as an active ingredient.

[0007] The present invention was derived from the above-mentioned needs, and provides an antioxidant and anti-inflammatory composition containing a carrot leaf lava seawater extract as an active ingredient, and confirms that the total polyphenol, rutin, and caffeic acid contents of the carrot leaf lava seawater extract of the present invention are increased and the antioxidant and anti-inflammatory activities are enhanced compared to the carrot leaf water extract, thereby completing the present invention.

[0008] In order to solve the above problem, the present invention provides an antioxidant and anti-inflammatory cosmetic composition containing carrot leaf lava seawater extract as an effective ingredient.

[0009] In addition, the present invention provides a health functional food composition for antioxidant and anti-inflammatory purposes containing carrot leaf lava seawater extract as an active ingredient.

[0010] In addition, the present invention provides an anti-inflammatory pharmaceutical composition containing carrot leaf lava seawater extract as an active ingredient.

[0011] In addition, the present invention provides an antioxidant and anti-inflammatory feed additive containing carrot leaf lava seawater extract as an effective ingredient.

[0012] In addition, the present invention provides a method for producing a carrot extract having increased contents of rutin, caffeic acid and total polyphenols and enhanced antioxidant and anti-inflammatory effects, comprising the step of adding water containing 10 to 20% (v / v) of demineralized lava seawater to pulverized carrot leaves and extracting the same.

[0013] The present invention relates to an antioxidant and anti-inflammatory composition containing a carrot leaf lava seawater extract as an active ingredient. The carrot leaf lava seawater extract of the present invention has increased total polyphenol, rutin, and caffeic acid contents compared to a carrot leaf water extract, has excellent DPPH radical scavenging activity, and has a remarkable effect of inhibiting NO production. Therefore, the carrot leaf lava seawater extract of the present invention can be usefully used as an antioxidant and anti-inflammatory composition.

[0014] Figure 1 shows the results of examining the total polyphenol content of carrot leaf extracts according to the content of demineralized lava seawater in the solvent. **** indicates that the total polyphenol content of carrot leaf 15% (v / v) and 30% (v / v) demineralized lava seawater extracts increased statistically significantly compared to carrot leaf water extract, with p<0.001.

[0015] Figure 2 shows the results of confirming the DPPH radical scavenging activity of carrot leaf extracts according to the content of demineralized lava seawater in the solvent. **** means that the DPPH radical scavenging activity of 15% (v / v) and 30% (v / v) demineralized lava seawater extracts of carrot leaves statistically significantly increased compared to the carrot leaf water extract, and p<0.001.

[0016] Figure 3 shows the results of confirming the NO production ability of carrot leaf extracts according to the content of demineralized lava seawater in the solvent. LPS is a substance that induces NO production. * indicates that the NO production inhibition ability of carrot leaf 15% (v / v) and 30% (v / v) demineralized lava seawater extracts was statistically significantly increased compared to carrot leaf water extract, and p<0.05.

[0017] Figure 4 shows the results of confirming the rutin and caffeic acid contents of carrot leaf extracts according to the content of demineralized lava seawater in the solvent. ***, **** indicate that the rutin or caffeic acid content of the 15% (v / v) demineralized lava seawater extract of carrot leaves statistically significantly increased compared to the carrot leaf water extract, and *** indicates p<0.005, and **** indicates p<0.001.

[0018] In order to achieve the purpose of the present invention, the present invention provides an antioxidant and anti-inflammatory cosmetic composition containing carrot leaf lava seawater extract as an effective ingredient.

[0019] The above carrot leaf lava seawater extract may be extracted using water containing 10 to 20% (v / v) of desalinated lava seawater as a solvent, preferably using water containing 13 to 17% (v / v) of desalinated lava seawater as a solvent, and more preferably using water containing 15% (v / v) of desalinated lava seawater as a solvent, but is not limited thereto.

[0020] The lava seawater of the present invention may be obtained by desalinating water collected at a depth of 30 to 150 m (based on average sea level) by excavating 100 to 200 m underground in the eastern region of Jeju Island, and preferably, by desalinating water collected at a depth of 100 to 150 m (based on average sea level) by excavating 100 to 200 m underground in Handong-ri, Gujwa-eup, Bukjeju-gun, eastern region of Jeju Island, and more preferably, by desalinating water collected at a depth of 130 m (based on average sea level) by excavating 100 to 200 m underground in Handong-ri, Gujwa-eup, Bukjeju-gun, eastern region of Jeju Island, but is not limited thereto.

[0021] The lava seawater of the present invention is preferably desalinated lava seawater obtained through one or more processes selected from the flash evaporation method, seawater freezing method, reverse osmosis method, ion exchange resin method, and electrodialysis method, and more preferably, it is obtained through an electrodialysis process in which ionized ions are separated using an ion exchange membrane using an electric current as a driving force from lava seawater taken from 130 m underground in the eastern part of Jeju, but is not limited thereto.

[0022] The desalinated lava seawater of the present invention has a hardness of 5000 mg / L or more, preferably a hardness of 5000 mg / L or more and 20000 mg / L or less, and more preferably a hardness of 5000 mg / L or more and 6000 mg / L or less, but is not limited thereto.

[0023] The above carrot leaf lava seawater extract has the characteristic of increasing the content of rutin and caffeic acid and the total polyphenol content compared to the carrot leaf water extract.

[0024] In a cosmetic composition according to one embodiment of the present invention, the cosmetic composition may be prepared in any one formulation selected from among a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion, a foundation, a wax foundation, and a spray, but is not limited thereto.

[0025] A cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these ingredients can be easily selected by a person skilled in the art.

[0026] When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component.

[0027] When the cosmetic composition of the present invention is in the form of a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane-butane or dimethyl ether may be additionally included.

[0028] When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

[0029] When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as a carrier component.

[0030] When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.

[0031] The cosmetic composition of the present invention may additionally contain excipients including fluorescent substances, fungicides, hydrotropism inducers, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.

[0032] In addition, the present invention provides a health functional food composition for antioxidant and anti-inflammatory purposes containing carrot leaf lava seawater extract as an active ingredient.

[0033] The above health functional food composition may be manufactured in any one formulation selected from powder, granules, pills, tablets, capsules, candy, syrup, and beverage, but is not limited thereto.

[0034] When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as is or used together with other foods or food ingredients, and may be used appropriately according to a conventional method. The amount of the active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). Generally, when manufacturing a food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

[0035] There are no specific restrictions on the types of the above health functional foods. Examples of foods to which the above health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea drinks, alcoholic beverages, and vitamin complexes, and include all health foods in the conventional sense.

[0036] In addition, the health functional food composition of the present invention can be manufactured into a food, particularly a functional food. The functional food of the present invention includes ingredients commonly added during food manufacturing, such as proteins, carbohydrates, fats, nutrients, and seasonings. For example, when manufactured into a drink, it may include natural carbohydrates or flavoring agents as additional ingredients in addition to the active ingredient. The natural carbohydrates are preferably monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, etc.), or sugar alcohols (e.g., xylitol, sorbitol, erythritol, etc.). The flavoring agents may be natural flavoring agents (e.g., thaumatin, stevia extract, etc.) and synthetic flavoring agents (e.g., saccharin, aspartame, etc.).

[0037] In addition to the above health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. may be further contained. The ratio of these added components is not particularly important, but is generally selected within the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health functional food composition of the present invention.

[0038] In addition, the present invention provides an anti-inflammatory pharmaceutical composition containing carrot leaf lava seawater extract as an active ingredient.

[0039] The pharmaceutical composition containing the above-described effective ingredient of the present invention may further contain an appropriate carrier, excipient or diluent commonly used in the manufacture of pharmaceutical compositions.

[0040] The pharmaceutical dosage form of the pharmaceutical composition of the present invention can be used alone or in combination with other pharmaceutically active compounds as well as in appropriate combinations.

[0041] The pharmaceutical composition according to the present invention can be formulated and used in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and injections, respectively, according to conventional methods. Carriers, excipients, and diluents that can be included in the pharmaceutical composition include various compounds or mixtures including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. When formulated, they are usually prepared using diluents or excipients such as fillers, bulking agents, binders, wetting agents, disintegrating agents, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, and capsules, and these solid preparations are prepared by mixing the active ingredient with at least one excipient, such as starch, calcium carbonate, sucrose or lactose, or gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups, and in addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspending agents can include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. Suppository bases can include withepsol, macrogol, Tween 61, cocoa butter, laurin butter, and glycerogelatin.

[0042] The preferred dosage of the pharmaceutical composition of the present invention varies depending on the patient's condition and weight, the severity of the disease, the drug form, the route and duration of administration, and can be appropriately selected by those skilled in the art. The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes. All modes of administration can be envisaged, and for example, it can be administered orally, rectally, or by intravenous, intramuscular, subcutaneous, intrauterine, or intracerebroventricular injection.

[0043] In addition, the present invention provides an antioxidant and anti-inflammatory feed additive containing carrot leaf lava seawater extract as an effective ingredient.

[0044] The feed additive of the present invention corresponds to supplementary feed under the Feed Management Act. The term "feed" in the present invention may mean any natural or artificial diet, meal, etc., or ingredients of the meal, which are intended for or suitable for animals to eat, ingest, and digest. The type of the feed is not particularly limited, and feed commonly used in the relevant technical field may be used. Non-limiting examples of the feed include plant feeds such as grains, roots, fruits, food processing by-products, algae, fibers, pharmaceutical by-products, oils and fats, starches, meal, or grain by-products; and animal feeds such as proteins, inorganic substances, oils and fats, mineral substances, oils and fats, single-cell proteins, zooplankton, or food. These may be used alone or in combination of two or more.

[0045] In addition, the present invention provides a method for producing a carrot extract having increased contents of rutin, caffeic acid and total polyphenols and enhanced antioxidant and anti-inflammatory effects, comprising the step of adding water containing 10 to 20% (v / v) of demineralized lava seawater to pulverized carrot leaves and extracting the same.

[0046] The above extraction may be high-temperature and pressure extraction at 110 to 130°C for 5 to 15 minutes, preferably high-temperature and pressure extraction at 120 to 125°C for 8 to 12 minutes, and more preferably high-temperature and pressure extraction at 121°C for 10 minutes, but is not limited thereto.

[0047] The carrot leaf powder of the present invention is preferably a powdered product of freeze-dried carrot leaves, but is not limited thereto.

[0048] It is preferable to extract by adding 80 to 120 mL of solvent to 1 g of the carrot leaf powder of the present invention, and more preferably to extract by adding 100 mL of solvent to 1 g of the carrot leaf powder, but the present invention is not limited thereto.

[0049] The method for producing a carrot extract with increased content of the above routine and enhanced antioxidant and anti-inflammatory effects may include, but is not limited to, adding a step of filtering with a filter paper, concentrating under reduced pressure, and then freeze-drying after the above step.

[0050]

[0051] Hereinafter, the present invention will be described in more detail using manufacturing examples and examples. These manufacturing examples and examples are intended solely to illustrate the present invention more specifically, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by them.

[0052]

[0053] Manufacturing Example 1. Manufacturing of carrot leaf extract according to the content of lava seawater in the solvent.

[0054] Carrot leaves were collected from Gujwa-eup, Jeju Island, freeze-dried, and then used.

[0055] The desalinated lava seawater of the present invention was provided and used from the Jeju Lava Seawater Center. Specifically, the desalinated lava seawater of the present invention was produced by using the principle of separating ions in lava seawater extracted from 130 m underground in the eastern part of Jeju Island using an ion exchange membrane with an electric current as a driving force, and the desalinated lava seawater of the present invention obtained through the electrodialysis process is a mineral water rich in calcium and magnesium and has the characteristic of a hardness of 5000 mg / L or more.

[0056] In order to prepare the carrot leaf lava seawater extract of the present invention, freeze-dried carrot leaves were ground with a grinder, and then 100 mL of solvent was added per 1 g of powder, and then high-temperature pressure extraction was performed at 121°C for 10 minutes using an autoclave, and the filtrate was filtered through a filter paper, concentrated under reduced pressure using a rotary vacuum concentrator, and then freeze-dried for 48 hours in a freeze dryer.

[0057] The solvent used was water not containing demineralized lava seawater (0%), water containing 15% (v / v) of demineralized lava seawater, or water containing 30% (v / v) of demineralized lava seawater.

[0058]

[0059] Example 1. Analysis of total polyphenol content

[0060] The total polyphenol content of the carrot leaf lava seawater extract prepared in Manufacturing Example 1 was analyzed.

[0061] Briefly, the total polyphenol content was determined by adding 125 μl of sample to 1,375 μl of distilled water, adding 0.5 mL of Folin-Ciocalteu's reagent, adding 1 mL of 10% (w / v) Na2CO3 after 3 minutes, and leaving it in the dark at room temperature for 30 minutes. The absorbance was measured at 700 nm using a spectrophotometer (Sunrise, Tecan, Salzburg, Austria). Gallic acid was used as a standard, and after creating a standard calibration curve, the total polyphenol content of the sample was expressed as gallic acid equivalents (mg GAE / g).

[0062] As a result, as disclosed in Fig. 1, it was confirmed that the total polyphenol content of the carrot leaf lava seawater extract increased compared to the carrot leaf water extract, and in particular, the total polyphenol content of the carrot leaf 15% (v / v) desalinated lava seawater extract was the highest.

[0063]

[0064] Example 2. Confirmation of antioxidant effect

[0065] The antioxidant effect of the carrot leaf lava seawater extract prepared in Manufacturing Example 1 was confirmed by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity.

[0066] Specifically, 160 ㎕ of 200 ㎕ DPPH dissolved in ethanol and 40 ㎕ of samples prepared at different concentrations were mixed and reacted at 37℃ in the dark for 30 minutes. Afterwards, the absorbance was measured at 517 nm, and the DPPH radical scavenging activity was evaluated using the following formula 1, and catechin was used as a positive control. All experiments were performed in triplicate, and EC was calculated using GraphPad Prism 7.0 software. 50 The value was calculated.

[0067] [Formula 1]

[0068] Radical scavenging activity (%) = (absorbance of control group - absorbance of extract treatment group) / absorbance of control group × 100

[0069]

[0070] As a result, as disclosed in Fig. 2, the DPPH radical scavenging activity of the carrot leaf extract treatment group increased in a concentration-dependent manner, and the DPPH radical scavenging activity of the carrot leaf lava seawater extract treatment group significantly increased at all concentrations compared to the carrot leaf water extract treatment group. In particular, when treated with 15% (v / v) desalinated lava seawater extract of carrot leaves, the DPPH radical scavenging activity was the best.

[0071] In addition, as disclosed in Table 1, the EC of the carrot leaf lava seawater extract treatment group was higher than that of the carrot leaf water extract treatment group. 50 The values ​​decreased, especially the EC of the group treated with 15% (v / v) demineralized lava seawater extract of carrot leaves 50 The value was the lowest.

[0072] Sample DPPH radical scavenging EC 50 Value (mg / mL) Carrot leaf water extract 7.29±0.71 Carrot leaf 15% (v / v) demineralized lava seawater extract 1.43±0.03 Carrot leaf 30% (v / v) demineralized lava seawater extract 2.01±0.01

[0073]

[0074] Example 3. Confirmation of anti-inflammatory effect

[0075] The anti-inflammatory effect of the carrot leaf extract of Manufacturing Example 1 was evaluated by analyzing the inhibition ability of nitric oxide (NO) production in Raw 264.7 cells activated with lipopolysaccharide (LPS).

[0076] LPS (Escherichia coli 026:B6; Difco, USA), Griess reagent, and sodium nitrite were purchased from Sigma (St. Louis, MO, USA). The mouse macrophage cell line Raw 264.7 was purchased from the Korean Cell Line Bank and cultured in DMEM (Gibco, USA) containing 10% (v / v) FBS, 10,000 units / mL penicillin, 10,000 μg / mL streptomycin, and 25 μg / mL amphotericin in a completely humidified cell incubator at 37°C and 5% carbon dioxide. All cells were tested at 80–90% confluence, and only cells that had not passed 20 passages were used.

[0077] The NO production inhibition ability was measured using the Griess analysis method after culturing Raw 264.7 cells (control group) that were not treated with the extract and Raw 264.7 cells (experimental group) that were pretreated with 800 ㎍ / mL of the sample of Preparation Example 1 for 2 hours and stimulated with 1 ㎍ / mL of LPS for 24 hours.

[0078] As a result, as disclosed in Fig. 3, the amount of NO production in the carrot leaf lava seawater extract treatment group decreased compared to the carrot leaf water extract treatment group, and in particular, the NO production reduction effect of the carrot leaf 15% (v / v) desalinated lava seawater extract treatment group was the best.

[0079]

[0080] Example 4. Analysis of rutin and caffeic acid content

[0081] The contents of rutin and caffeic acid in carrot leaf water extract and carrot leaf 15% (v / v) demineralized lava seawater extract with the best antioxidant and anti-inflammatory effects were compared and analyzed using HPLC (High-performance liquid chromatography).

[0082] As a result, as disclosed in Fig. 4, it was confirmed that the rutin and caffeic acid contents in the 15% (v / v) desalinated lava seawater extract of carrot leaves increased compared to the carrot leaf water extract.

Claims

1. An antioxidant and anti-inflammatory cosmetic composition containing carrot leaf lava seawater extract as an active ingredient.

2. An antioxidant and anti-inflammatory cosmetic composition according to claim 1, wherein the carrot leaf lava seawater extract is extracted using water containing 10 to 20% (v / v) of desalinated lava seawater as a solvent.

3. An antioxidant and anti-inflammatory cosmetic composition according to claim 1, characterized in that the desalinated lava seawater is obtained through one or more processes selected from the flash evaporation method, seawater freezing method, reverse osmosis method, ion exchange resin method, and electrodialysis method.

4. An antioxidant and anti-inflammatory cosmetic composition according to claim 1, wherein the carrot leaf lava seawater extract has an increased content of rutin, caffeic acid, and total polyphenol.

5. A health functional food composition for antioxidant and anti-inflammatory purposes containing carrot leaf lava seawater extract as an active ingredient.

6. In the fifth paragraph, the health functional food composition is characterized in that it is manufactured in any one formulation selected from powder, granules, pills, tablets, capsules, candy, syrup, and beverage.

7. Anti-inflammatory pharmaceutical composition containing carrot leaf lava seawater extract as an active ingredient.

8. Antioxidant and anti-inflammatory feed additive containing carrot leaf lava seawater extract as an active ingredient.

9. A method for producing a carrot extract having increased contents of rutin, caffeic acid and total polyphenols and enhanced antioxidant and anti-inflammatory effects, comprising the step of adding water containing 10 to 20% (v / v) of demineralized lava seawater to crushed carrot leaves and extracting the same.

10. A method for producing a carrot extract having increased contents of rutin, caffeic acid and total polyphenols and enhanced antioxidant and anti-inflammatory effects, characterized in that the extraction in claim 9 is performed by high-temperature and pressure extraction at 110 to 130°C for 5 to 15 minutes.

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