Expression vector systems with two copies of light chain
The expression system with two vectors for multi-specific antibodies, featuring a common light chain and optimized promoters, addresses the high cost and complexity of manufacturing by enhancing titers and quality, making these antibodies more accessible.
Patent Information
- Application Number
- PCT/US2025/011957
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-01-19
- Filing Date
- 2025-01-17
- Publication Date
- 2025-07-24
AI Technical Summary
The high cost and complexity of manufacturing multi-specific antibodies, particularly those with three unique antibody chains, hinder their widespread adoption as front-line therapies due to the need for optimized cell lines, large-scale culturing, and purification processes, which are not adequately addressed by existing expression vector systems.
An expression system comprising two expression vectors, each with a common or identical light chain and separate heavy chain or Fc fusion, optimized with specific promoters and polyA signal sequences, to produce multi-specific antibodies with either one Fab or two identical Fabs, enhancing production titers and product quality.
This approach increases production titers and improves product quality by balancing chain expression, reducing impurities, and lowering manufacturing costs, making multi-specific antibodies more accessible and cost-effective.