Anti-c5 antibody fused to factor h for use in the treatment of complement-mediated diseases

Abstract

The present application provides novel dosing regimens for methods of treating a complement-mediated disease in a human individual, comprising administering to the individual an effective amount of a fusion protein comprising i) an antibody moiety that specifically binds to human C5 and ii) a Factor H (FH) or functional fragment thereof. The complement-mediated disease can be, for example, paroxysmal nocturnal hemoglobinuria (PNH) syndrome, C3 glomerulopathy (C3G), IgA nephropathy (IgAN), thrombotic microangiopathy secondary to systemic lupus erythematosus (SLE-TMA), and thrombotic microangiopathy secondary to lupus nephritis (LN-TMA).

Description

Attorney Docket No.792252001440 METHODS OF TREATING COMPLEMENT-MEDIATED DISEASES CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This patent application claims priority benefits of U.S. Provisional Application No. 63 / 641,863 filed on May 2, 2024, the content of which is incorporated herein by reference in its entirety. REFERENCE TO AN ELECTRONIC SEQUENCE LISTING

[0002] The contents of the electronic sequence listing (792252001440SEQLIST.xml; Size: 153,100 bytes; and Date of Creation: April 28, 2025) is herein incorporated by reference in its entirety. FIELD OF THE INVENTION

[0003] The present invention relates to compositions and methods for treating complement- mediated diseases. BACKGROUND OF THE INVENTION

[0004] The complement system is part of innate immunity that plays a key role in host defense. It enhances (complements) the ability of antibodies and phagocytic cells to clear microbes and damaged cells from an organism, promote inflammation, and attack pathogen’s cell membrane. However, activated complement also has the potential to cause significant tissue injury and destruction and dysregulated complement activity has been found to be associated with a number of rare and common diseases such as paroxysmal nocturnal hemoglobinuria (PNH), atypical hemolytic uremic syndrome (aHUS), rheumatoid arthritis (RA), age-related macular degeneration (AMD), etc. Thus, anti-complement therapy is a promising way of treating these human disorders.

[0005] Complement activation involves a cascade of target recognition and proteolytic cleavage. It can be activated via three different pathways, all of them converge at the C3 activation step. These pathways are the classical, alternative and lectin pathways. The Classical Pathway (CP) is activated by antigen-antibody complex and involves the sequential activation of C1 and C4 / C2 before merging with other pathways at the C3 activation step. The lectin pathway (LP) is triggered by certain pattern recognition molecules, such as mannose-binding lectin (MBL), collectins and ficolins upon their binding to microbial surface sugar molecules. It involves the activation of mannan-binding lectin serine proteases (MASPs) which then cleave C4 / C2 and join the other pathways at the C3 activation step. The alternative pathway (AP) is constitutively active at a low level due to spontaneous hydrolysis 1sf-6711731Attorney Docket No.792252001440 and activation of C3 to produce C3(H2O). The latter can associate with factor B, and upon proteolytic activation by factor D, produces the initial C3 cleaving enzyme complex C3(H2O)Bb. In the absence of regulatory proteins, the product of C3(H2O)Bb complex, C3b, can associate with factor B in the same way as C3(H2O) does, and thus starts another cycle of self-amplifying C3 activation.

[0006] Ongoing efforts to target C3 activation in complement-dependent diseases employ C3-inhibitory cyclic peptides or recombinant short variants of FH, which have very poor pharmacokinetics and require large and frequent (e.g., daily) dosing.

[0007] C3 activation also leads to the generation of C5-cleaving enzyme complexes and initiates the terminal complement activation pathway, culminating in the production of the potent pro-inflammatory mediator C5a and the membrane attack complex (MAC) C5b-9 which can cause cell lysis and death. To prevent complement from causing indiscriminate injury, host cells express a number of membrane-anchored regulators that function to block complement activation and amplification. Some of these regulators, including decay- accelerating factor (DAF, CD55) and MCP, work to inhibit C3 activation, while others such as CD59 work at other steps of the complement activation cascade. In addition to membrane- anchored complement regulators, there are also fluid phase regulators in the blood which act to preferentially protect the host tissues. The fluid phase inhibitors include factor H (FH) and factor I (FI), which are critical inhibitors of the alternative pathway of complement activation, and C4BP and C1 inhibitor (C1INH) which inhibit the classical pathway complement activation. Both fluid phase and membrane-anchored complement regulatory proteins are often composed of multiple conserved SCR domains. For example, FH is composed of 20 SCRs.

[0008] Complement C5 is a critical protein in the terminal pathway of complement activation and is the precursor protein for generating the potent pro-inflammatory mediator C5a, as well as the cytolytic membrane attack complex (MAC).

[0009] A number of human inflammatory and autoimmune diseases are mediated by C5a and / or MAC, and blocking C5 activation should prevent C5a and MAC generation and be of therapeutic value. A humanized mouse anti-human C5 monoclonal antibody (mAb), eculizumab (e.g., Soliris®), has been used to treat two complement-mediated diseases paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). However, not all PNH patients are responsive to eculizumab treatments and one of the reasons for non-responsiveness is genetic polymorphism of human C5 with loss of epitope binding to eculizumab. Additionally, due to high plasma concentration of C5 and 2sf-6711731Attorney Docket No.792252001440 targeted-mediated rapid removal of antibody, eculizumab has to be administered to patients at high doses and frequency. Thus, more effective and more convenient anti-complement drugs are needed, both in the treatment of PNH and aHUS, and in other complement-mediated diseases.

[0010] The disclosures of all publications, patents, patent applications and published patent applications referred to herein are hereby incorporated herein by reference in their entirety. BRIEF SUMMARY OF THE INVENTION

[0011] The present invention in one aspect provides a method of treating a complement- mediated disease in a human individual, comprising administering to the human individual an effective amount of a fusion protein comprising i) an antibody moiety that specifically binds to human C5 (“anti-C5 antibody moiety”) and ii) a Factor H (FH) or functional fragment thereof, wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, and wherein: a) the human individual has a body weight of less than 80 kg, and the fusion protein is administered at a maintenance dose of about 1,920 mg at the maintenance phase; or b) the human individual has a body weight of 80 kg or greater, and the fusion protein is administered at a maintenance dose of about 2400 mg at the maintenance phase.

[0012] In some embodiments according to the method described above, the fusion protein is administered intravenously (IV) at the initial phase and is administered subcutaneously (SC) at the maintenance phase.

[0013] In some embodiments according to any one of the methods described above, the initial phase comprises administering a single initial dose of the fusion protein on Day 1, and the maintenance phase comprises administering a maintenance dose of the fusion protein biweekly (Q2W) starting Day 8 for at least about 13 weeks. In some embodiments, the human individual’s body weight is less than 80 kg, and the maintenance dose of the fusion protein is about 1,920 mg. In some embodiments, the human individual’s body weight is 80 kg or greater, and the maintenance dose of the fusion protein is about 2,400 mg. In some embodiments, the initial phase comprises administering the fusion protein at a single initial dose of about 2,400 mg IV.

[0014] In some embodiments according to any one of the methods described above, the human individual’s body weight is less than 80 kg, and wherein the method comprises administering the fusion protein at a single initial dose of about 2,400 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 1,920 mg 3sf-6711731Attorney Docket No.792252001440 SC biweekly starting Day 8 for at least about 13 weeks for the maintenance phase. In some embodiments, the human individual’s body weight is 80 kg or greater, and wherein the method comprises administering the fusion protein at a single initial dose of about 2,400 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 2,400 mg SC biweekly starting Day 8 for at least about 13 weeks for the maintenance phase.

[0015] In certain aspects, provided herein is a method of treating a complement-mediated disease in a human individual, comprising administering to the human individual an effective amount of a fusion protein comprising i) an antibody moiety that specifically binds to human C5 (“anti-C5 antibody moiety”) and ii) a Factor H (FH) or functional fragment thereof, wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, and wherein the fusion protein is administered at a maintenance dose of about 200 mg to about 720 mg at the maintenance phase.

[0016] In some embodiments according to the method described above, the fusion protein is administered intravenously (IV) at the initial phase and is administered intravenously (IV) or subcutaneously (SC) at the maintenance phase.

[0017] In some embodiments according to any one of the methods described above, the fusion protein is administered weekly (QW) or biweekly (Q2W) at the maintenance phase. In some embodiments, the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg IV on Day 1. In some embodiments, the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of: i) about 270 mg IV to about 720 mg IV biweekly; or ii) about 270 mg SC to about 720 mg SC weekly.

[0018] In some embodiments according to any one of the methods described above, the maintenance phase is at least about 12 weeks.

[0019] In some embodiments according to any one of the methods described above, the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg IV on Day 1, and wherein (i) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 300 mg SC to about 540 mg SC weekly for at least 12 weeks; or (ii) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 270 mg IV to about 450 mg IV biweekly for at least about 13 weeks.

[0020] In some embodiments according to any one of the methods described above, the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg IV on Day 1, and wherein: (i) the maintenance phase comprises administering the fusion 4sf-6711731Attorney Docket No.792252001440 protein starting Day 8 at a maintenance dose of about 300 mg SC weekly for at least about 12 weeks; (ii) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 420 mg SC weekly for at least about 12 weeks; (iii) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 540 mg SC weekly for at least about 12 weeks; (iv) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 270 mg IV biweekly for at least about 13 weeks; (v) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 360 mg IV biweekly for at least about 13 weeks; or (vi) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 450 mg IV biweekly for at least about 13 weeks.

[0021] In some embodiments according to any one of the methods described above, the complement-mediated disease is selected from the group consisting of paroxysmal nocturnal hemoglobinuria (PNH) syndrome, C3 glomerulopathy (C3G), IgA nephropathy (IgAN), thrombotic microangiopathy secondary to systemic lupus erythematosus (SLE-TMA), and thrombotic microangiopathy secondary to lupus nephritis (LN-TMA). In some embodiments, the complement-mediated disease is PNH. In some embodiments, the complement-mediated disease is IgAN.

[0022] In some embodiments according to any one of the methods described above, the binding of the anti-C5 antibody moiety to human C5 is pH-dependent, and the anti-C5 antibody moiety binds more strongly to human C5 at a neutral pH (e.g., about pH 7.4; such as that found in the blood) than it does at an acidic pH (e.g., about pH 5.8; such as that found in the endosome).

[0023] In some embodiments according to any one of the methods described above, the anti- C5 antibody moiety is a full-length antibody, a Fab, a Fab’, a F(ab)2, a F(ab’)2, an scFv, or a combination thereof.

[0024] In some embodiments according to any one of the methods described above, the anti- C5 antibody moiety is a full-length antibody (“anti-C5 full-length antibody”). In some embodiments, the anti-C5 full-length antibody comprises an Fc fragment derived from human IgG4. In some embodiments, the Fc fragment comprises the amino acid sequence of any of SEQ ID NOs: 32, 33, and 61, such as SEQ ID NO: 61. In some embodiments, the anti-C5 full-length antibody comprises a heavy chain and a light chain, wherein (i) the heavy chain comprises the amino acid sequence of SEQ ID NO: 119, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (ii) the heavy chain comprises the amino acid 5sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 121, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (iii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 123, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (iv) the heavy chain comprises the amino acid sequence of SEQ ID NO: 125, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (v) the heavy chain comprises the amino acid sequence of SEQ ID NO: 120, and the light chain comprises the amino acid sequence of SEQ ID NO: 90; (vi) the heavy chain comprises the amino acid sequence of SEQ ID NO: 122, and the light chain comprises the amino acid sequence of SEQ ID NO: 90; (vii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 124, and the light chain comprises the amino acid sequence of SEQ ID NO: 90; or (viii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 126, and the light chain comprises the amino acid sequence of SEQ ID NO: 90.

[0025] In some embodiments according to any one of the methods described above, the fusion protein inhibits C3 activation.

[0026] In some embodiments according to any one of the methods described above, the functional fragment of FH comprises short consensus repeat (SCR) domains 1-5 of the FH protein. In some embodiments, the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 85.

[0027] In some embodiments according to any one of the methods described above, the fusion protein comprises a first FH or functional fragment thereof and a second FH or functional fragment thereof, the first FH or functional fragment thereof is fused to the C- terminus of a first heavy chain of the anti-C5 full-length antibody, and the second FH or functional fragment thereof is fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody. In some embodiments, (i) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 72, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (ii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 76, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (iii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 78, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (iv) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 80, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (v) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89, each light chain comprises the amino acid sequence of SEQ ID NO: 90; (vi) 6sf-6711731Attorney Docket No.792252001440 each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 116, each light chain comprises the amino acid sequence of SEQ ID NO: 90; (vii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 117, each light chain comprises the amino acid sequence of SEQ ID NO: 90; or (viii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 118, each light chain comprises the amino acid sequence of SEQ ID NO: 90.

[0028] In some embodiments according to any one of the methods described above, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0.

[0029] The methods described herein can use any of the fusion proteins described herein. Anti-C5 antibody moieties and the FH moieties are described in more details below. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] FIGs.1A-1C depict graphs of the mean (± standard deviation) hemoglobin increase from baseline of complement inhibitor-naïve PNH patients administered the anti-C5-FH fusion protein across 58 weeks for Cohorts 1, 2, and 3. The horizonal dashed line represents hemoglobin normalization at 12 g / dL. The vertical dash on the lines represents a switch in optimal biological dose (OBD). Data points to the left of the vertical dash represent pre-OBD hemoglobin measurements. Data points to the right of the vertical dash represent post-OBD hemoglobin measurements.

[0031] FIGs.2A-2C depict graphs of the mean (± standard deviation) lactate dehydrogenase (LDH) levels of complement inhibitor-naïve PNH patients administered the anti-C5-FH fusion protein across 58 weeks for Cohorts 1, 2, and 3. The top, horizontal dashed line represents total LDH that is 1.5 times the upper limit of normal (ULN). The lower, horizonal dashed line represents the total LDH that is 1 time the ULN. Various data points are marked to indicate LDH levels measured post-OBD.

[0032] FIG.3 shows summary of safety data in all treated patients.

[0033] FIG.4 shows patient demographics and baseline characteristics. 7sf-6711731Attorney Docket No.792252001440 DETAILED DESCRIPTION OF THE INVENTION

[0034] The present application provides novel dosing regimens for methods of treating complement-mediated diseases through inhibition of complement signaling using an anti- C5 / factor H fusion protein (hereinafter referred to as “anti-C5-FH fusion protein”) comprising an anti-C5 antibody moiety and a factor H (FH) moiety. Anti-C5-FH fusion proteins used herein inhibit complement system activities via dual mechanisms: i) the anti-C5 antibody moiety functions as an anti-C5 antibody to block C5 activity; and ii) the FH moiety acts as a C3 complement activation inhibitor. In some embodiments, the anti-C5 antibody moiety exhibits pH-dependent binding to C5 (hereinafter referred to as “pH-dependent anti- C5 antibody moiety”). In some embodiments, the pH-dependent anti-C5 antibody moiety binds more strongly to C5 at a more neutral pH (e.g., about pH 7.4; such as that found in the blood) than it does at a more acidic pH (e.g., about pH 5.8; such as that found in the endosome). In some embodiments, the FH moiety is FH protein or fragment thereof, such as an FH fragment comprising short consensus repeat (SCR) domains 1-5 of a FH protein, which are domains involved in regulating C3 activation. Complement-mediated diseases include, but are not limited to, paroxysmal nocturnal hemoglobinuria (PNH) syndrome, C3 glomerulopathy (C3G), IgA nephropathy (IgAN), thrombotic microangiopathy secondary to systemic lupus erythematosus (SLE-TMA), and thrombotic microangiopathy secondary to lupus nephritis (LN-TMA). Anti-C5-FH fusion proteins and anti-C5 antibody moieties (e.g., pH-dependent anti-C5 antibody moieties) have been described in US Patent Publication No. 20220204602, International Application Nos. WO2020 / 219922 and WO2024097796, the entire contents of each of which is incorporated herein by reference in their entirety. Treatment methods described herein provide well-tolerated, more effective, and more convenient ways to treat complement-dependent pathologies (e.g., PNH), such as by reducing dosing amount and / or frequency, selecting a dose by assessing the body weight of a human individual (e.g., whether the body weight is less than 80 kg or not), and / or by more effectively blocking complement system activities, which addresses and meets needs previously unmet by other complement-mediated disease therapies (e.g., FDA-approved eculizumab). I. Definitions

[0035] In general, terms used in the claims and the specification are intended to be construed as having the plain meaning understood by a person of ordinary skill in the art. Certain terms 8sf-6711731Attorney Docket No.792252001440 are defined below to provide additional clarity. In case of conflict between the plain meaning and the provided definitions, the provided definitions are to be used.

[0036] The terms “inhibit” and “inhibition,” as used herein, means to reduce, suppress, diminish or block an activity or function by at least about 10% (e.g., at least about any of 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100%) relative to a control value. In some embodiments, the activity is suppressed or blocked by at least about 50% compared to a control value. In some embodiments, the activity is suppressed or blocked by at least about 75%. In some embodiments, the activity is suppressed or blocked by at least about 95%. In some embodiments, the activity is 100% blocked.

[0037] The terms “effective amount” and “pharmaceutically effective amount” refer to a sufficient amount of an agent to provide the desired biological result. That result can be reduction (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100%) and / or alleviation of the signs, symptoms, or causes of a disease or disorder, or any other desired alteration of a biological system. An appropriate effective amount in any individual case may be determined by one of ordinary skill in the art using routine experimentation.

[0038] The terms “patient,” “subject,” “individual,” and the like are used interchangeably herein, and refer to any animal, in some embodiments a mammal, and in some embodiments a human, having a complement system, including a human in need of therapy for, or susceptible to, a condition or its sequelae. The individual may include, for example, dogs, cats, pigs, cows, sheep, goats, horses, rats, rabbits, hamsters, guinea pigs, monkeys, mice, and humans. In some embodiments, the individual is a human.

[0039] The term “abnormal” when used in the context of organisms, tissues, cells or components thereof, refers to those organisms, tissues, cells or components thereof that differ in at least one observable or detectable characteristic (e.g., age, treatment, time of day, etc.) from those organisms, tissues, cells or components thereof that display the “normal” (expected / homeostatic) respective characteristic. Characteristics which are normal or expected for one cell, tissue type, or subject, might be abnormal for a different cell or tissue type.

[0040] A “disease” is a state of health of a subject wherein the subject cannot maintain homeostasis, and wherein if the disease is not ameliorated then the subject's health continues to deteriorate.

[0041] In contrast, a “disorder” in a subject is a state of health in which the subject is able to maintain homeostasis, but in which the subject's state of health is less favorable than it would 9sf-6711731Attorney Docket No.792252001440 be in the absence of the disorder. Left untreated, a disorder does not necessarily cause a further decrease in the subject's state of health.

[0042] A disease or disorder is “alleviated” if the severity of a sign or symptom of the disease or disorder, the frequency with which such a sign or symptom is experienced by a patient, or both, is reduced.

[0043] As used herein, the term “treatment” refers to clinical intervention designed to alter the natural course of the individual or cell being treated during the course of clinical pathology. Desirable effects of treatment include decreasing the rate of disease progression, ameliorating or palliating the disease state, and remission or improved prognosis. For example, an individual is successfully “treated” if one or more symptoms associated with disease or disorder are mitigated or eliminated, including, but not limited to, decreasing the frequency and / or severity of a sign and / or symptoms resulting from the disease, increasing the quality of life of those suffering from the disease, decreasing the dose of other medications required to treat the disease, and / or prolonging survival of individuals. Treatment may be prophylactic (to prevent or delay the onset of the disease, or to prevent the manifestation of clinical or subclinical symptoms thereof) or therapeutic suppression or alleviation of symptoms after the manifestation of the disease.

[0044] A subject who “may be suitable”, which includes a subject who “is suitable” for treatment(s) described herein, is a subject who is more likely than not to benefit from administration of said treatments. Conversely, a subject who “may not be suitable” or “may be unsuitable”, which includes a subject who is “unsuitable” for treatment(s) described herein, is a subject who is more likely than not to fail to benefit from administration of said treatments.

[0045] An “effective amount” or “therapeutically effective amount” of a compound is that amount of compound which is sufficient to provide a beneficial effect to the subject to which the compound is administered.

[0046] A “therapeutic treatment” is a treatment administered to a subject who exhibits signs of disease or disorder, for the purpose of diminishing or eliminating those signs.

[0047] The term “antibody,” as used herein, refers to an immunoglobulin molecule which is able to specifically bind to a specific epitope of an antigen. Antibodies can be intact immunoglobulins derived from natural sources, or from recombinant sources and can be immunoreactive portions of intact immunoglobulins. The antibodies in the present invention may exist in a variety of forms including, for example, polyclonal antibodies, monoclonalantibodies, intracellular antibodies (“intrabodies”), Fv, Fab, Fab , F(ab)2, and F(ab )2, as well10sf-6711731Attorney Docket No.792252001440 as single chain antibodies (scFv), heavy chain antibodies, such as camelid antibodies, and humanized antibodies (Harlow et al., 1999, Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY; Harlow et al., 1989, Antibodies: A Laboratory Manual, Cold Spring Harbor, N.Y.; Houston et al., 1988, Proc. Natl. Acad. Sci. USA 85:5879-5883; Bird et al., 1988, Science 242:423-426).

[0048] As used herein, the term “heavy chain antibody” or “heavy chain antibodies” comprises immunoglobulin molecules derived from camelid species, either by immunization with a peptide and subsequent isolation of sera, or by the cloning and expression of nucleic acid sequences encoding such antibodies. The term “heavy chain antibody” or “heavy chain antibodies” further encompasses immunoglobulin molecules isolated from a subject with heavy chain disease, or prepared by the cloning and expression of VH (variable heavy chain immunoglobulin) genes from a subject.

[0049] A “chimeric antibody” refers to a type of engineered antibody which contains a naturally-occurring variable region (light chain and heavy chains) derived from a donor antibody in association with light and heavy chain constant regions derived from an acceptor antibody.

[0050] A “humanized antibody” refers to a type of engineered antibody having its CDRs derived from a non-human donor immunoglobulin, the remaining immunoglobulin-derived parts of the molecule being derived from one (or more) human immunoglobulin(s). In addition, framework support residues may be altered to preserve binding affinity (see, e.g., 1989, Queen et al., Proc. Natl. Acad Sci USA, 86:10029-10032; 1991, Hodgson et al., Bio / Technology, 9:421). A suitable human acceptor antibody may be one selected from a conventional database, e.g., the KABAT database, Los Alamos database, and Swiss Protein database, by homology to the nucleotide and amino acid sequences of the donor antibody. A human antibody characterized by a homology to the framework regions of the donor antibody (on an amino acid basis) may be suitable to provide a heavy chain constant region and / or a heavy chain variable framework region for insertion of the donor CDRs. A suitable acceptor antibody capable of donating light chain constant or variable framework regions may be selected in a similar manner. It should be noted that the acceptor antibody heavy and light chains are not required to originate from the same acceptor antibody. The prior art describes several ways of producing such humanized antibodies (see for example EP-A-0239400 and EP-A-054951).

[0051] “CDRs” are defined as the complementarity determining region amino acid sequences of an antibody which are the hypervariable regions of immunoglobulin heavy and light 11sf-6711731Attorney Docket No.792252001440 chains. See, e.g., Kabat et al., Sequences of Proteins of Immunological Interest, 4th Ed., U.S. Department of Health and Human Services, National Institutes of Health (1987). There are three heavy chain and three light chain CDRs (or CDR regions) in the variable portion of an immunoglobulin. Thus, “CDRs” as used herein refers to all three heavy chain CDRs, or all three light chain CDRs (or both all heavy and all light chain CDRs, if appropriate). The structure and protein folding of the antibody may mean that other residues are considered part of the antigen binding region and would be understood to be so by a skilled person. See for example Chothia et al., (1989) Conformations of immunoglobulin hypervariable regions; Nature 342, p 877-883.

[0052] The terms “native antibody,” “full-length antibody,” “intact antibody,” and “whole antibody” are used herein interchangeably to refer to an antibody in its substantially intact form, not antibody fragments as defined below. The terms particularly refer to an antibody with heavy chains that contain an Fc region. Native antibodies are usually heterotetrameric glycoproteins of about 150,000 Daltons, composed of two identical light (L) chains and two identical heavy (H) chains. Each light chain is linked to a heavy chain by one covalent disulfide bond, while the number of disulfide linkages varies among the heavy chains of different immunoglobulin isotypes. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain has at one end a variable domain (VH) followed by a number of constant domains. Each light chain has a variable domain at one end (VL) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Particular amino acid residues are believed to form an interface between the light chain and heavy chain variable domains.

[0053] The “variable region” or “variable domain” of an antibody refers to the amino- terminal domains of the heavy or light chain of the antibody. The variable domain of the heavy chain may be referred to as “VH.” The variable domain of the light chain may be referred to as “VL.” These domains are generally the most variable parts of an antibody and contain the antigen-binding sites.

[0054] The term “variable” refers to the fact that certain portions of the variable domains differ extensively in sequence among antibodies and are used in the binding and specificity of each particular antibody for its particular antigen. However, the variability is not evenly distributed throughout the variable domains of antibodies. It is concentrated in three segments called hypervariable regions (HVRs, also referred to as CDRs) both in the light-chain and the heavy-chain variable domains. The more highly conserved portions of variable domains are 12sf-6711731Attorney Docket No.792252001440 called the framework regions (FR). The variable domains of native heavy and light chains each comprise four FR regions, largely adopting a beta-sheet configuration, connected by three HVRs, which form loops connecting, and in some cases forming part of, the beta-sheet structure. The HVRs in each chain are held together in close proximity by the FR regions and, with the HVRs from the other chain, contribute to the formation of the antigen-binding site of antibodies (see Kabat et al., Sequences of Proteins of Immunological Interest, Fifth Edition, National Institute of Health, Bethesda, Md. (1991)).

[0055] The term “constant domain” refers to the portion of an immunoglobulin molecule having a more conserved amino acid sequence relative to the other portion of the immunoglobulin, the variable domain, which contains the antigen binding site. The constant domain contains the CH1, CH2 and CH3 domains (collectively, CH) of the heavy chain and the CL domain of the light chain. The constant domains are not involved directly in the binding of an antibody to an antigen, but exhibit various effector functions, such as participation of the antibody in antibody-dependent cellular toxicity.

[0056] The “light chains” of antibodies (immunoglobulins) from any mammalian species canbe assigned to one of two clearly distinct types, called kappa (“ ”) and lambda (“ ”), basedon the amino acid sequences of their constant domains.

[0057] The term IgG “isotype” or “subclass” as used herein is meant any of the subclasses of immunoglobulins defined by the chemical and antigenic characteristics of their constant regions.

[0058] Depending on the amino acid sequences of the constant domains of their heavy chains, antibodies (immunoglobulins) can be assigned to different classes. There are five major classes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM, and several of these may be further divided into subclasses (isotypes), e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. The heavy chain constant domains that correspond to the different classes of immunoglobulins are called , , , , and μ, respectively. The subunit structures and three-dimensional configurations of different classes of immunoglobulins are well known and described generally in, for example, Abbas et al. Cellular and Mol. Immunology, 4th ed. (W.B. Saunders, Co., 2000). An antibody may be part of a larger fusion molecule, formed by covalent or non-covalent association of the antibody with one or more other proteins or peptides.

[0059] “Antibody fragments” comprise a portion of an intact antibody, preferably comprising the antigen binding region thereof. In some embodiments, the antibody fragment described herein is an antigen binding fragment. Examples of antibody fragments or antigen binding 13sf-6711731Attorney Docket No.792252001440 fragments include Fab, Fab’, F(ab’)2, and Fv fragments (such as single-chain variable fragment, scFv); diabodies; linear antibodies; single-chain antibody molecules; and multispecific antibodies formed from antibody fragments.

[0060] Papain digestion of antibodies produces two identical antigen-binding fragments, called “Fab” fragments, each with a single antigen-binding site, and a residual “Fc” fragment, whose name reflects its ability to crystallize readily. Pepsin treatment yields an F(ab’)2fragment that has two antigen-combining sites and is still capable of cross-linking antigen.

[0061] “Fv” is the minimum antibody fragment which contains a complete antigen-binding site. In some embodiments, a two-chain Fv species consists of a dimer of one heavy- and one light-chain variable domain in tight, non-covalent association. In a single-chain Fv (scFv) species, one heavy- and one light-chain variable domain can be covalently linked by a flexible peptide linker such that the light and heavy chains can associate in a “dimeric” structure analogous to that in a two-chain Fv species. It is in this configuration that the three HVRs of each variable domain interact to define an antigen-binding site on the surface of the VH-VL dimer. Collectively, the six HVRs confer antigen-binding specificity to the antibody. However, even a single variable domain (or half of an Fv comprising only three HVRs specific for an antigen) has the ability to recognize and bind antigen, although at a lower affinity than the entire binding site.

[0062] The Fab fragment has two polypeptide chains, containing the heavy- and light-chain variable domains (VH, VL), and also containing the constant domain of the light chain (CL) and the first constant domain (CH1) of the heavy chain. Fab’ fragments differ from Fab fragments by the addition of a few residues at the carboxy terminus of the heavy chain CH1 domain including one or more cysteines from the antibody hinge region. Fab’-SH is the designation herein for Fab’ in which the cysteine residue(s) of the constant domains bear a free thiol group. F(ab’)2 antibody fragments originally were produced as pairs of Fab’ fragments which have hinge cysteines between them. Other chemical couplings of antibody fragments are also known.

[0063] “Single-chain Fv” or “scFv” antibody fragments comprise the VH and VL domains of antibody, wherein these domains are present in a single polypeptide chain. Generally, the scFv polypeptide further comprises a polypeptide linker between the VH and VL domains which enables the scFv to form the desired structure for antigen binding. For a review of scFv, see, e.g., Pluckthün, The Pharmacology of Monoclonal Antibodies. Springer Berlin Heidelberg, 1994.269-315. 14sf-6711731Attorney Docket No.792252001440

[0064] The “Fc” fragment comprises the carboxy-terminal portions of both heavy chains held together by di-sulfides. The effector functions of antibodies are determined by sequences in the Fc region, which region is also the part recognized by Fc receptors (FcR) found on certain types of cells.

[0065] The term “monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, e.g., the individual antibodies comprising the population are identical except for possible mutations, e.g., naturally occurring mutations, that may be present in minor amounts. Thus, the modifier “monoclonal” indicates the character of the antibody as not being a mixture of discrete antibodies. In some embodiments, such a monoclonal antibody typically includes an antibody comprising a polypeptide sequence that binds a target, wherein the target-binding polypeptide sequence was obtained by a process that includes the selection of a single target binding polypeptide sequence from a plurality of polypeptide sequences. For example, the selection process can be the selection of a unique clone from a plurality of clones, such as a pool of hybridoma clones, phage clones, or recombinant DNA clones. It should be understood that a selected target binding sequence can be further altered, for example, to improve affinity for the target, to humanize the target binding sequence, to improve its production in cell culture, to reduce its immunogenicity in vivo, to create a multispecific antibody, etc., and that an antibody comprising the altered target binding sequence is also a monoclonal antibody of this invention. In contrast to polyclonal antibody preparations, which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody of a monoclonal antibody preparation is directed against a single determinant on an antigen. In addition to their specificity, monoclonal antibody preparations are advantageous in that they are typically uncontaminated by other immunoglobulins.

[0066] The modifier “monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method. For example, the monoclonal antibodies to be used in accordance with the invention may be made by a variety of techniques, including, for example, the hybridoma method (e.g., Kohler and Milstein, Nature 256:495-97 (1975); Hongo et al., Hybridoma 14 (3): 253-260 (1995), Harlow et al., Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed.1988); Hammerling et al., Monoclonal Antibodies and T-Cell Hybridomas 563-681 (Elsevier, N.Y., 1981)), recombinant DNA methods (see, e.g., U.S. Pat. No.4,816,567), phage-display technologies (see, e.g., Clackson et al., Nature 352: 624-628 (1991); Marks et al., J. Mol. 15sf-6711731Attorney Docket No.792252001440 Biol.222: 581-597 (1992); Sidhu et al., J. Mol. Biol.338(2): 299-310 (2004); Lee et al., J. Mol. Biol.340(5): 1073-1093 (2004); Fellouse, Proc. Natl. Acad. Sci. USA 101(34): 12467- 12472 (2004); and Lee et al., J. Immunol. Methods 284(1-2): 119-132 (2004)), and technologies for producing human or human-like antibodies in animals that have parts or all of the human immunoglobulin loci or genes encoding human immunoglobulin sequences (see, e.g., WO 1998 / 24893; WO 1996 / 34096; WO 1996 / 33735; WO 1991 / 10741; Jakobovits et al., Proc. Natl. Acad. Sci. USA 90: 2551 (1993); Jakobovits et al., Nature 362: 255-258 (1993); Bruggemann et al., Year in Immunol.7:33 (1993); U.S. Pat. Nos.5,545,807; 5,545,806; 5,569,825; 5,625,126; 5,633,425; and 5,661,016; Marks et al., Bio / Technology 10: 779-783 (1992); Lonberg et al., Nature 368: 856-859 (1994); Morrison, Nature 368: 812- 813 (1994); Fishwild et al., Nature Biotechnol.14: 845-851 (1996); Neuberger, Nature Biotechnol.14: 826 (1996); and Lonberg and Huszar, Intern. Rev. Immunol.13: 65-93 (1995)).

[0067] The monoclonal antibodies (mAbs) herein specifically include “chimeric” antibodies in which a portion of the heavy and / or light chain is identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (see, e.g., U.S. Pat. No.4,816,567; and Morrison et al., Proc. Natl. Acad. Sci. USA 81:6851-6855 (1984)). Chimeric antibodies include PRIMATTZED® antibodies wherein the antigen-binding region of the antibody is derived from an antibody produced by, e.g., immunizing macaque monkeys with the antigen of interest.

[0068] The structures and locations of immunoglobulin variable regions may be determined by reference to Kabat, E. A. et al., Sequences of Proteins of Immunological Interest.4th Edition. US Department of Health and Human Services.1987, and updates thereof, now available on the Internet (immuno.bme.nwu.edu).

[0069] By the term “specifically binds,” as used herein with respect to an antibody, is meant an antibody which recognizes and binds to a specific target molecule, but does not substantially recognize or bind other molecules in a sample. In some instances, the terms “specific binding” or “specifically binding,” is used to mean that the recognition and binding is dependent upon the presence of a particular structure (e.g., an antigenic determinant or epitope) on the target molecule. If, for example, an antibody specifically binds to epitope 16sf-6711731Attorney Docket No.792252001440 “A,” the presence of an unlabelled molecule containing epitope A (or free, unlabeled A) in a reaction containing labeled “A” and the antibody, will reduce the amount of labeled A bound to the antibody. An antibody that binds to or specifically binds to a target (e.g., an epitope) is an antibody that binds this target with greater affinity, avidity, more readily, and / or with greater duration than it binds to other targets. In some embodiments, the extent of binding of an antibody to an unrelated target is less than about 10% of the binding of the antibody to the target as measured, e.g., by a radioimmunoassay (RIA). In some embodiments, an antibodythat specifically binds to a target has a dissociation constant (Kd) of 1 M, 100 nM, 10nM, 1 nM, or 0.1 nM. In some embodiments, an antibody specifically binds to an epitope on a protein that is conserved among the protein from different species. In some embodiments, specific binding can include, but does not require exclusive binding.

[0070] As used herein, the term “affinity” refers to the strength of interaction between antibody and antigen at single antigenic sites. Within each antigenic site, the variable region of the antibody “arm” interacts through weak non-covalent forces with antigen at numerous sites; the more interactions, the stronger the affinity.

[0071] The term “multispecific” as used in conjunction with an antibody or antigen binding protein refers to an antibody or antigen binding protein having polyepitopic specificity (i.e., is capable of specifically binding to two, three, or more, different epitopes on one biological molecule or is capable of specifically binding to epitopes on two, three, or more, different biological molecules).

[0072] As used herein, “Percent (%) amino acid sequence identity” and “homology” with respect to a peptide, polypeptide or antibody sequence are defined as the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the specific peptide or polypeptide sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN or MEGALIGNTM(DNASTAR) software. Those skilled in the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared.

[0073] An amino acid substitution may include but is not limited to the replacement of one amino acid in a polypeptide with another amino acid. Exemplary substitutions are shown in Table A. Amino acid substitutions may be introduced into an antibody of interest and the 17sf-6711731Attorney Docket No.792252001440 products screened for a desired activity, e.g., retained / improved antigen binding, decreased immunogenicity, or improved ADCC or CDC. TABLE A

[0074] Amino acids may be grouped according to common side-chain properties: (1) hydrophobic: Norleucine, Met, Ala, Val, Leu, Ile; (2) neutral hydrophilic: Cys, Ser, Thr, Asn, Gln; (3) acidic: Asp, Glu; (4) basic: His, Lys, Arg; (5) residues that influence chain orientation: Gly, Pro; (6) aromatic: Trp, Tyr, Phe. Non-conservative substitutions will entail exchanging a member of one of these classes for another class.

[0075] The term “covalently linked” as used herein, refers to a direct linkage through one or more chemical bonds or an indirect linkage through one or more linkers. Any suitable chemical bond can be used to create a direct linkage, including but not limited to, a covalent bond such as a peptide bond and a disulfide bond, or a non-covalent bond such as a hydrogen bond, a hydrophobic bond, an ionic bond, or a van der Waals bond.

[0076] As used herein, the “C terminus” of a polypeptide refers to the last amino acid residue of the polypeptide which donates its amine group to form a peptide bond with the carboxyl group of its adjacent amino acid residue. “N terminus” of a polypeptide as used herein refers to the first amino acid of the polypeptide which donates its carboxyl group to form a peptide bond with the amine group of its adjacent amino acid residue.

[0077] Ranges: throughout this disclosure, various aspects of the invention can be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope 18sf-6711731Attorney Docket No.792252001440 of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 2.7, 3, 4, 5, 5.3, and 6. This applies regardless of the breadth of the range.

[0078] It is understood that embodiments of the invention described herein include “consisting of” and / or “consisting essentially of” embodiments.

[0079] As used herein, reference to “not” a value or parameter generally means and describes “other than” a value or parameter. For example, the method is not used to treat cancer of type X means the method is used to treat cancer of types other than X.

[0080] Reference to “about” a value or parameter herein includes (and describes) variations that are directed to that value or parameter per se. For example, description referring to “about X” includes description of “X”. The term “about X-Y” used herein has the same meaning as “about X to about Y.”

[0081] As used herein and in the appended claims, the singular forms “a,” “or,” and “the” include plural referents unless the context clearly dictates otherwise. II. Methods of treatment

[0082] This invention relates to the inhibition of the complement signaling and complement- related diseases or disorders in an individual (e.g., human) using an anti-C5-FH fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an anti-C5 antibody moiety that specifically binds to C5 (e.g., human C5) and ii) an FH or functional fragment thereof, and provides novel dosing regimens described herein for the administration of the anti-C5-FH fusion protein. This invention also relates to the inhibition of the complement signaling and complement-related diseases or disorders in an individual (e.g., human) using any of the anti- C5-FH fusion proteins and dosing regimens described herein. In some embodiments, the anti- C5 antibody moiety exhibits pH-dependent binding to C5 (e.g., human C5). In some embodiments, the pH-dependent anti-C5 antibody moiety binds more strongly to C5 at a more neutral pH (e.g., about pH 7.4; such as that found in the blood) than it does at a more acidic pH (e.g., about pH 5.8; such as that found in the endosome). Such pH-dependent binding provides for greater persistence of administered antibody molecules, because immune complexes (i.e., anti-C5 mAb bound to C5) taken up by cells will dissociate in the acidic environment of the endosome and allow the freed antibody to be recycled back out of 19sf-6711731Attorney Docket No.792252001440 the cell through the neonatal Fc receptor (FcRn) where it is available to bind to a new C5 molecule. In some embodiments, the individual is a complement inhibitor-naïve individual.

[0083] In some embodiments, the method described herein inhibits the complement signaling cascade by specifically targeting complement component C5 protein, or a fragment of the protein C5a or C5b, such as by inhibiting (e.g., inhibiting at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100%) C5a-mediated inflammation and cell activation, or inhibiting C5b-mediated cell lysis. In some embodiments, the method described herein inhibits the complement signaling cascade by specifically targeting complement component C3b protein, such as for preventing (e.g., preventing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100%) complement C3b deposition and amplification. In some embodiments, methods of treating and preventing inflammation and autoimmune diseases mediated by unwanted, uncontrolled, excessive complement activation are provided. In some embodiments, the method is for the treatment of complement-mediated disease or complement-mediated disorder in an individual (e.g., human) by administering to the individual (e.g., intravenously or subcutaneously) an anti-C5- FH fusion protein (e.g., FMEH-IgG4-PLA-FH). In some embodiments, the method is for the treatment of complement-mediated disease or complement-mediated disorder in an individual (e.g., human) by administering to the individual (e.g., intravenously or subcutaneously) an anti-C5 antibody moiety (such as any of the anti-C5 antibodies or antigen-binding fragments thereof described herein).

[0084] Any complement-related diseases, such as diseases related to C3 (or C3b) and / or C5 (or C5a, C5b) activities, or AP and / or terminal pathway activities, can be treated using the methods described herein. Defective complement action is a cause of several human glomerular diseases including atypical hemolytic uremic syndrome (aHUS), anti-neutrophil cytoplasmic antibody mediated vasculitis (ANCA), C3 glomerulopathy, IgA nephropathy, immune complex membranoproliferative glomerulonephritis, renal ischemic reperfusion injury, lupus nephritis, membranous nephropathy, and chronic transplant mediated glomerulopathy. Aberrant complement component activation has also been proposed as markers in various types of cancers and their clinical outcomes. Lung cancer patients show significantly higher plasma levels of complement proteins and activation fragments than do control donors, and elevated complement levels are correlated with lung tumor size. Complement-related proteins are also elevated in biological fluids from patients with other types of tumor. See, for example, Pio et al. Semin Immunol.2013 Feb; 25(1): 54–64. 20sf-6711731Attorney Docket No.792252001440 Inhibition of the complement cascade has been proposed for glomerular diseases and cancer treatment.

[0085] In some embodiments, the complement-related disease is selected from the group consisting of: macular degeneration (MD), age-related macular degeneration (AMD), ischemia reperfusion injury, arthritis, rheumatoid arthritis, asthma, allergic asthma, lupus, ulcerative colitis, stroke, post-surgery systemic inflammatory syndrome, chronic obstructive pulmonary disease (COPD), PNH syndrome, myasthenia gravis, neuromyelitis optica, (NMO), multiple sclerosis, delayed graft function, antibody-mediated rejection, aHUS, central retinal vein occlusion (CRVO), central retinal artery occlusion (CRAO), epidermolysis bullosa, sepsis, organ transplantation, inflammation (including, but not limited to, inflammation associated with cardiopulmonary bypass surgery and kidney dialysis), C3 glomerulopathy (C3G), membranous nephropathy, IgA nephropathy (IgAN), glomerulonephritis (including, but not limited to, ANCA-mediated glomerulonephritis, lupus nephritis, and combinations thereof), thrombotic microangiopathies secondary to systemic lupus erythematosus (SLE-TMA), ANCA-mediated vasculitis, shiga toxin induced HUS, and antiphospholipid antibody-induced pregnancy loss, thrombotic microangiopathy secondary to lupus nephritis (LN-TMA), or any combinations thereof. In some embodiments, the AP- mediated disease is C3G. In some embodiments, the AP-mediated disease is macular degeneration, such as AMD.

[0086] In some embodiments, the method is for treating a complement-mediated disease or disorder in an individual (e.g., human), comprising the step of administering to said human individual an anti-C5 antibody moiety (e.g., any of anti-C5 antibodies or antigen-binding fragments thereof described herein), thereby inhibiting the generation of a C5a or C5b protein, and formation of MAC. Examples of complement-mediated diseases that can be treated using the methods of the invention include, but are not limited to PNH syndrome, C3G, IgAN, SLE-TMA, and LN-TMA.

[0087] In some embodiments, the method comprises administering the anti-C5-FH fusion protein at a high initial dose at the initial phase, such as about 600 mg to about 3600 mg (including for example any of 600, 1200, 1440, 1800, 1920, 2400, 2880 mg, and 3600 mg, or any ranges in between), in order to mitigate the impact of target-mediated drug disposition (TMDD). Pharmacokinetics of mAbs have revealed that higher antigen mass is associated with higher mAb clearance, which lengthens the time to reach steady-state drug concentration (see, e.g., Ternant et al., Influence of Antigen Mass on the Pharmacokinetics of Therapeutic Antibodies in Humans. Clinical Pharmacokinetics, 2019 Feb;58(2)). Use of high initial dose 21sf-6711731Attorney Docket No.792252001440 upfront can decrease the contribution of clearance due to the nonlinear TMDD to the total clearance (nonlinear clearance due to the TMDD + linear nonspecific clearance), so that the steady-state drug concentration can be reached sooner. Weight-tiered Dosing Regimen

[0088] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., PNH) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, and wherein the maintenance dose of the fusion protein at the maintenance phase is determined by the human individual’s body weight (e.g., whether the body weight is less than 80 kg or not). In some embodiments, the fusion protein is administered intravenously (IV) or subcutaneously (SC), e.g., at initial phase and / or maintenance phase. In some embodiments, the method comprises administering the fusion protein at one or more (e.g., one) initial doses at the initial phase, followed by administering the fusion protein (e.g., weekly, biweekly, every three weeks, every four weeks, every five weeks, every six weeks, or longer) for two or more (e.g., 2, 3, 4, 5, 6, 8, 10, 11, 12, 13, 14, 23, 24, 25, 26, 40, 44, 47, 48, 49, 50, 60, 70, 80, 90, 100, or more, or lifelong) maintenance doses at the maintenance phase. In some embodiments, the complement-mediated disease is selected from the group consisting of PNH, C3G, IgAN, SLE-TMA, and LN-TMA, such as PNH. In some embodiments, the initial dose is about 1200 mg IV to about 3600 mg IV, such as any of about 1880 mg IV to about 2880 mg IV, about 2200 mg IV to about 2400 mg IV, about 2300 mg IV to about 2500 mg IV, about 1200 mg IV, about 3600 mg IV, about 2000 mg IV, or about 2400 mg IV. In some embodiments, the initial phase comprises administering the fusion protein at a single initial dose of about 1880 mg IV to about 2880 mg IV, such as any of about 1880 mg IV, about 1920 mg IV, about 2000 mg IV, about 2200 mg IV, about 2400 mg IV, about 2600 mg IV, about 2800 mg IV, or about 2880 mg IV. In some embodiments, the maintenance dose is any of about 720 mg to about 2880 mg, about 720 mg to about 1920 mg, about 1400 mg to about 2880 mg, about 1400 mg to about 2400 mg, about 2000 mg to about 2880 mg, about 1920 mg to about 2880 mg, about 1920 mg to about 2400 mg, about 1800 mg to about 2400 mg, about 1400 mg to about 1920 mg, about 1400 mg to about 1800 mg, about 1400 mg to about 1600 mg, about 1600 mg to about 1800 22sf-6711731Attorney Docket No.792252001440 mg, or about 1800 mg to about 1920 mg. In some embodiments, the maintenance dose is any of about 720 mg, about 1400 mg, about 1600 mg, about 1800 mg, about 1920 mg, about 2400 mg, or about 2880 mg. In some embodiments, the two or more maintenance doses of the fusion protein are administered biweekly (Q2W), every 15 days, every 16 days, every 17 days, every 18 days, every 19 days, every 20 days, every three weeks (Q3W), every four weeks (Q4W), every five weeks (Q5W), every six weeks (Q6W), or longer interval. In some embodiments, the two or more maintenance doses of the fusion protein are each administered (e.g., biweekly, Q3W, or with longer interval) at about 1400 mg SC to about 2880 mg SC, such as any of about 1400 mg SC to about 2400 mg SC, about 1400 mg SC to about 1920 mg SC, about 2000 mg SC to about 2400 mg SC, about 2000 mg SC to about 2880 mg SC, about 1400 mg SC, about 1600 mg SC, about 1800 mg SC, about 1920 mg SC, about 2000 mg SC, about 2200 mg SC, about 2400 mg SC, or about 2880 mg SC. In some embodiments, the maintenance phase is at least about 2 weeks, such as at least about any of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 34, 36, 38, 40, 42, 44, 45, 46, 47, 48, 49, 50, 52, 64, 84 weeks, 2 years, 3 years, 4 years, 6 years, 8 years, 10 years, 20 years, 30 years or longer, or lifelong. In some embodiments, the maintenance phase is at least about 12 weeks, such as at least about any of 12 weeks, 13 weeks, 24 weeks, 25 weeks, 48 weeks, 49 weeks, 64 weeks, 84 weeks, or 2 years. In some embodiments, the maintenance phase is at least about 12 weeks (e.g., 12, 13, 14, 15, 16, 18, 20, 22, 23, 24, 25, 26, 28, 30, 34, 38, 40, 42, 44, 46, 47, 48, 49, 50 weeks or more, such as 12 weeks) for weekly maintenance dosing, or at least about 13 weeks (e.g., 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, weeks, 2, 3, 4, 6, 8, 10, 20, 30, years or longer, or lifelong, such as 84 weeks) for biweekly maintenance dosing. In some embodiments, the maintenance phase is at least about 24 weeks (e.g., 24 weeks) for weekly maintenance dosing, or at least about 25 weeks (e.g., 25 weeks, or 84 weeks) for biweekly maintenance dosing. In some embodiments, the maintenance phase is at least about 12 weeks (e.g., 84 weeks) for Q3W, Q4W, Q5W, Q6W, or longer interval than Q6W, maintenance dosing. In some embodiments, the maintenance dosing at the maintenance phase is lifelong. In some embodiments, the method further comprises assessing the body weight of the human individual (e.g., before the initial phase starts, or before the maintenance phase starts). In some embodiments, the method further comprises selecting a maintenance dose of the fusion protein at the maintenance phase, wherein the method comprises assessing the body weight of the human individual, wherein i) when the human individual has a body weight of less than 23sf-6711731Attorney Docket No.792252001440 80 kg, the maintenance dose of the fusion protein is selected as about 1400 mg to about 2400 mg (e.g., about 1920 mg) (e.g., SC, and / or biweekly, Q3W, Q4W, or longer interval) at the maintenance phase; and ii) when the human individual has a body weight of 80 kg or greater, the maintenance dose of the fusion protein is selected as about 2000 mg to about 2880 mg (e.g., about 2400 mg) (e.g., SC, and / or biweekly, Q3W, Q4W, or longer interval) at the maintenance phase. In some embodiments, the maintenance dose for a human individual with a body weight of 80 kg or greater is higher (e.g., at least about any of 20%, 50%, 75%, 1-fold, 1.25-fold, 1.5-fold, 2-fold, 5-fold, or more higher) than the maintenance dose for a human individual with a body weight of less than 80 kg. In some embodiments, the human individual has a body weight of less than 80 kg (such as less than any of 79 kg, 75 kg, 70 kg, 65 kg, 60 kg, 55 kg, 50 kg, 45 kg, or 40 kg), and the fusion protein is administered at a maintenance dose of about 1400 mg to about 2400 mg (e.g., about 1400 mg to about 1920 mg, about 1400 mg to about 1800, about 1400 mg to about 1600 mg, about 1600 mg to about 1920 mg, about 1600 mg to about 2200 mg, about 1800 mg to about 2000 mg, about 1800 mg to about 1920, about 1900 mg to about 1920 mg, such as about 1920 mg) (e.g., SC, and / or biweekly, Q3W, Q4W, or longer interval) at the maintenance phase. In some embodiments, the human individual has a body weight of 80 kg or greater (such as greater than any of 85 kg, 90 kg, 95 kg, 100 kg, 105 kg, 110 kg, 115 kg, or 120 kg), and the fusion protein is administered at a maintenance dose of about 2000 mg to about 2880 mg (e.g., about 2000 mg to about 2400 mg, about 2000 mg to about 2200 mg, about 2200 mg to about 2400 mg, about 2200 mg to about 2600 mg, about 2350 mg to about 2500 mg, such as about 2400 mg) (e.g., SC, and / or biweekly, Q3W, Q4W, or longer interval) at the maintenance phase. In some embodiments, the human individual has a body weight of about 40 kg to less than 80 kg (e.g., about 45 kg to about 79 kg), and the fusion protein is administered at a maintenance dose of about 1920 mg SC Q2W (or Q3W, Q4W, or longer interval) at the maintenance phase. In some embodiments, the human individual has a body weight of about 80 kg to about 120 kg, and the fusion protein is administered at a maintenance dose of about 2400 mg SC Q2W (or Q3W, Q4W, or longer interval) at the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid 24sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is a full-length antibody comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L- Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0089] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., PNH) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, wherein the method comprises administering the fusion protein at a single initial dose of about 1880 mg IV to about 2880 mg IV (e.g., about any of 1920 mg IV, 2400 mg IV, or 2880 mg IV) for the initial phase, and administering the fusion protein at: a) a maintenance dose of about 1400 mg SC to about 2400 mg SC (e.g., about any of 1400 mg SC, 1440 mg SC, 1600 mg SC, 1800 mg SC, 1920 mg SC, or 2000 mg SC) biweekly (Q2W) (or Q3W, Q4W, or longer interval) starting Day 8 for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong) for the maintenance phase, wherein the human individual’s body weight is less than 80 kg, or b) a maintenance dose of about 2000 mg SC to about 2880 mg SC (e.g., about any of 2000 mg SC, 2200 mg SC, 2400 mg SC, or 2880 mg SC) biweekly (Q2W) (or Q3W, Q4W, or longer interval) starting Day 8 for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong) for the maintenance phase, wherein the human individual has a body weight of 80 kg or greater. In some embodiments, 25sf-6711731Attorney Docket No.792252001440 the maintenance dose for a human individual with a body weight of 80 kg or greater is higher (e.g., at least about any of 20%, 50%, 75%, 1-fold, 1.25-fold, 1.5-fold, 2-fold, 5-fold, or more higher) than the maintenance dose for a human individual with a body weight of less than 80 kg. In some embodiments, the method further comprises assessing the body weight of the human individual (e.g., before the initial phase starts, or before the maintenance phase starts). In some embodiments, the initial phase comprises administering the fusion protein at a single initial dose of about 2400 mg IV on Day 1, and the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 1400 mg SC to about 2400 mg SC (e.g., about 1920 mg SC) biweekly (or Q3W, Q4W, or longer interval) for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong), wherein the human individual has a body weight of less than 80 kg. In some embodiments, the initial phase comprises administering the fusion protein at a single initial dose of about 2400 mg IV on Day 1, and the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 2000 mg SC to about 2880 mg SC (e.g., about 2400 mg SC) biweekly (or Q3W, Q4W, or longer interval) for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong), wherein the human individual has a body weight of 80 kg or greater. In some embodiments, the initial phase comprises administering the fusion protein at a single initial dose of about 1880 mg IV to about 2880 mg IV (e.g., about 2400 mg IV) on Day 1, and the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 1920 mg SC biweekly (or Q3W, Q4W, or longer interval) for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong), wherein the human individual has a body weight of less than 80 kg. In some embodiments, the initial phase comprises administering the fusion protein at a single initial dose of about 1880 mg IV to about 2880 mg IV (e.g., about 2400 mg IV) on Day 1, and the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 2400 mg SC biweekly (or Q3W, Q4W, or longer interval) for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong), wherein the human individual has a body weight of 80 kg or greater. In some embodiments, the anti-C5 antibody moiety comprises a VH- CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL 26sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is a full-length antibody comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0090] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., PNH) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, wherein the method comprises administering the fusion protein at a single initial dose of about 720 mg IV to about 1400 mg IV (e.g., about any of 1000 mg IV, 1200 mg IV, or 1400 mg IV, such as 1200 mg IV) for the initial phase, and administering the fusion protein starting Day 8 at a first maintenance dose of about 600 mg SC to about 1200 mg SC (e.g., about any of 720 mg SC, 800 mg SC, or 1000 mg SC, such as 720 mg SC) weekly for at least about 12 weeks (e.g., no more than about 40 weeks) followed by administering the fusion protein at a second maintenance dose for at least about 48 weeks for the maintenance phase; wherein: i) the second maintenance dose is administered at about 1400 mg SC to about 2400 mg SC (e.g., about 1920 mg SC) biweekly (or Q3W, Q4W, or longer interval) when the human individual has a body weight of less than 80 kg; or ii) the second maintenance dose is administered at about 2000 mg SC to about 2880 mg SC (e.g., about 2400 mg SC) biweekly (or Q3W, Q4W, or longer interval) when the human individual 27sf-6711731Attorney Docket No.792252001440 has a body weight of 80 kg or greater. In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., PNH) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH- IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, wherein the method comprises administering the fusion protein at a single initial dose of about 2880 mg IV to about 4000 mg IV (e.g., about any of 3000 mg IV, 3600 mg IV, or 3800 mg IV, such as 3600 mg IV) for the initial phase, and administering the fusion protein starting Day 8 at a first maintenance dose of about 2400 mg SC to about 3600 mg SC (e.g., about any of 2600 mg SC, 2880 mg SC, or 3200 mg SC, such as 2880 mg SC) biweekly for at least about 13 weeks (e.g., no more than about 40 weeks) followed by administering the fusion protein at a second maintenance dose for at least about 48 weeks for the maintenance phase; wherein: i) the second maintenance dose is administered at about 1400 mg SC to about 2400 mg SC (e.g., about 1920 mg SC) biweekly (or Q3W, Q4W, or longer interval) when the human individual has a body weight of less than 80 kg; or ii) the second maintenance dose is administered at about 2000 mg SC to about 2880 mg SC (e.g., about 2400 mg SC) biweekly (or Q3W, Q4W, or longer interval) when the human individual has a body weight of 80 kg or greater. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is a full- length antibody comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some 28sf-6711731Attorney Docket No.792252001440 embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration. In some embodiments, the method further comprises assessing the body weight of the human individual (e.g., before the initial phase starts, or before the maintenance phase starts).

[0091] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., PNH) in a human individual having a body weight of less than 80 kg (e.g., about 45 kg to about 79 kg), comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; and wherein the fusion protein is administered at a single initial dose of about 2400 mg IV on Day 1 for the initial phase, and is administered at a maintenance dose of about 1400 mg SC to about 1920 mg SC (e.g., about 1440 mg SC, about 1600 mg SC, about 1800 mg SC, about 1920 SC, such as about 1,920 mg SC) biweekly (or Q3W, Q4W, or longer interval) starting Day 8 for at least about 13 weeks (e.g., at least about 84 weeks or longer, or lifelong) for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL- CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is 29sf-6711731Attorney Docket No.792252001440 fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C- terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full- length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the method further comprises assessing the body weight of the human individual (e.g., before the initial phase starts, or before the maintenance phase starts). In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0092] In some embodiments, there is provided a method of treating complement-mediated disease (e.g., PNH) in a human individual having a body weight of 80 kg or greater (e.g., about 80 kg to about 120 kg), comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 2,400 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 2000 mg SC to about 2400 mg SC (e.g., about 2,400 mg SC) biweekly (or Q3W, Q4W, or longer interval) starting Day 8 for at least about 13 weeks (e.g., at least about 84 weeks or loner, or lifelong) for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid 30sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti- C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the method further comprises assessing the body weight of the human individual (e.g., before the initial phase starts, or before the maintenance phase starts). In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration. PNH 31sf-6711731Attorney Docket No.792252001440

[0093] Complement-mediated hemolytic anemia contributes to numerous disorders of the red blood cells, such as paroxysmal nocturnal hemoglobinuria (PNH). PNH is a hematological disorder characterized by the clonal expansion of one or a few hematopoietic stem cells which are incapable of glycosylphosphatidylinositol (GPI)-anchor biosynthesis, due to an acquired somatic mutation in the phosphatidylinositol glycan class A (PIG-A) gene. Affected progeny cells are deficient in all GPI-anchored surface proteins, including complement regulators CD55 and CD59. Thus, PNH red blood cells (RBCs) are vulnerable to activated complement, and particularly to the membrane attack complex (MAC), resulting in chronic intravascular hemolysis with recurrent exacerbations.

[0094] PNH is a condition in which uncontrolled complement activity leads to systemic complications, principally through intravascular hemolysis and platelet activation (see Socie G, et al., French Society of Haematology. Lancet.1996;348(9027):573-577 and Brodsky, R., Blood.2014;124(18):2804-2811). Persistent intravascular hemolysis may be triggered by various stressors, such as infection or physical exertion, and this leads to smooth muscle contraction (free hemoglobin), chronic anemia, and an increased risk of severe thromboembolism. Thromboembolism is the most common cause of mortality in patients with PNH, and pulmonary hypertension and end-organ damage of vital organs, such as the liver, kidneys, brain, and intestines, are sequelae of such events (Hillmen, P., et al, Am. J. Hematol.2010;85(8):553-559). Due to these adverse pathologic processes, patients with PNH have a decreased quality of life (QoL), which may include debilitating fatigue, chronic pain, poor physical function, shortness of breath, abdominal pain, erectile dysfunction, a need for anti-coagulation, blood transfusions and in some instances, need for dialysis (Weitz, IC., et al., Thromb Res.2012;130(3):361-368). Patients with PNH are at risk of substantial morbidity and mortality.

[0095] PNH patients can exhibit at least one of the following characteristics, which characteristics may be symptoms of residual anemia and / or complement-mediated extravascular hemolysis (EVH) and / or incomplete control of intravascular hemolysis: a) exhibits signs or symptoms continued loss of RBCs by ongoing or intermittent intravascular hemolysis and / or extravascular hemolysis; b) has RBCs opsonized by fragments of C3; c) requires periodic blood transfusions; d) has low normal or below normal levels of hemoglobin; e) has low normal or below normal levels of platelets; f) has high normal or above normal reticulocytes; g) has high normal or above normal bilirubin; or h) has iron overload or is at risk of iron overload. 32sf-6711731Attorney Docket No.792252001440

[0096] The above characteristics can also be used to monitor the PNH patients’ progress in response to treatment in accordance with the present invention, and to modify the dosage regime if deemed clinically appropriate. In certain embodiments, the subject having PNH has previously been treated with a terminal complement inhibitor, but persists in exhibiting at least one of the above characteristics.

[0097] In some embodiments, the method of treating PNH described herein can prevent (e.g., preventing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% chance), delay (e.g., delaying at least about any of 1, 2, 3, 6, 12, 18, 24, 30, 36, 42, 48, 52 months or longer), or reduce (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) the severity of one or more of PNH signs / symptoms / characteristics discussed above, including but not limited to, EVH, fatigue, abdominal pain, dyspnea, anemia, dysphagia, chest pain, pallor, jaundice, cytopenia, and erectile dysfunction. In some embodiments, the method of treating PNH described herein results in a reduction (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) in one or more of the following in the subject: (a) persistent EVH; (b) anemia; (c) transfusion dependence; (d) intravascular hemolysis; (e) uncontrolled C3 activation and opsonization; and (f) the occurrence of “breakthrough” hemolytic crises observed in patients treated with terminal complement inhibitors. In some embodiments, the method of treating PNH described herein results in an improvement (e.g., improving at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) in one or more of the following in the subject: i) FACIT-Fatigue Scale Score; ii) serum LDH and hemoglobin (HgB) levels; iii) quality of life; iv) absolute reticulocyte count; v) bilirubin levels, and vi) haptoglobin levels. In some embodiments, the method of treating PNH described herein reduces (e.g., reducing at least about any of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) in one or more of the following: (a) C3b deposition and (b) plasma free C5 levels. In some embodiments, the method of treating PNH described herein reduces (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) lactate dehydrogenase (LDH) levels compared to baseline. In some embodiments, the method of treating PNH described herein reduces LDH levels to below 0.5 times, 1.0 times, or 1.5 times the upper limit of normal (ULN). In some embodiments, the method of treating PNH described herein increases (e.g., increasing at least about any of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) HgB levels compared to baseline. In some embodiments, the method of treating PNH described herein achieves an Hgb increase from baseline by at least 33sf-6711731Attorney Docket No.792252001440 about any of 2 g / dL, 3 g / dL, 4 g / dL, 5 g / dL, 6 g / dL, 7 g / dL, 8 g / dL, 9 g / dL, 10 g / dL, 11 g / dL, or 12 g / dL. In some embodiments, the method of treating PNH described herein increases (e.g., increasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) the proportion of PNH red blood cells which are able to survive complement attack. In some embodiments, the human individual to be treated has extravascular hemolysis (EVH). Thus, in some embodiments, the disclosure also relates to method of treating clinically- evident EVH in a human individual suffering from PNH.

[0098] In some embodiments, the efficacy of the method of treating PNH described herein can be assessed by one or more of: i) increase (e.g., 2 g / dL increase) in hemoglobin level from baseline (e.g., in the absence of transfusion); ii) decrease (e.g., decreasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in serum lactate dehydrogenase (LDH) levels; iii) proportion of subjects with breakthrough hemolysis defined as at least 1 new or worsening symptom or sign of intravascular hemolysis (fatigue, hemoglobinuria, abdominal pain, shortness of breath [dyspnea], anemia [hemoglobin < 10 g / dL], MAVE including thrombosis, dysphagia, or erectile dysfunction) in the presence of elevated LDH 2 × the upper limit of normal (ULN), after prior LDH reduction to < 1.5 × ULN on therapy; iv) change in the proportion of subjects with hemoglobin 12 g / dL; v) increase (e.g., increasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) in quality of life assessed by the Functional Assessment of Chronic Illness Therapy (FACIT)- Fatigue Score and EQ-5D-3L; vi) decrease from baseline the number of needed transfusions; and vii) increase in mean score for a healthy population. In some embodiments, the efficacy of the method of treating PNH described herein can further be assessed by one or more of: i) tolerance of the anti-C5-FH-fusion protein by absence of treatment emergent adverse events (TEAEs) of grade 3 or higher; ii) no adverse events (AEs) leading to discontinuation of the anti-C5-FH-fusion protein treatment or death; and iii) maintaining >12g / dL Hgb levels for at least 4 weeks following end of treatment. The pharmacodynamics and biomarker changes can also be measured to reflect treatment efficacy, including but are not limited to: 1) decrease (e.g., decreasing at least about any of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in C3b activity assay; 2) decrease (e.g., decreasing at least about any of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in total and free serum C5 levels; 3) change from baseline in rabbit RBC assay; 4) change from baseline in Factor H serum level; 5) change from baseline in d-dimer; 6) increase (e.g., increasing at least about any of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 34sf-6711731Attorney Docket No.792252001440 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in free hemoglobin; 7) increase (e.g., increasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in serum total and direct bilirubin; 8) increase (e.g., increasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in serum haptoglobin levels; and 9) increase (e.g., increasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) from baseline in reticulocyte count.

[0099] In some embodiments, the method of treating PNH described herein further comprises selecting a human individual suitable for such treatment. In some embodiments, the human individual to be treated are complement inhibitor-naïve subjects with PNH. In some embodiments, the human individual to be treated are complement inhibitor-naïve subjects with PNH on LDH, hemoglobin and transfusion dependence. In some embodiments, the human individual further receives antibiotic prophylaxis during the treatment. In some embodiments, the human individual is at least about 18 years old. In some embodiments, the human individual meets one or more of the criteria: 1) diagnosis of PNH confirmed by flow cytometry evaluation of white blood cells and red blood cells, e.g., with granulocyte or monocyte clone size of 10% within 6 months of screening; 2) presence of 1 or more PNH- related signs or symptoms, e.g., within 3 months of screening; 3) LDH 2.0 × ULN at screening; 4) hemoglobin 10.0 g / dL at screening; 5) practice effective contraception during treatment; 6) negative pregnancy test for females during treatment; 7) BMI of < 35 kg / m2; 8) prior vaccination against Neisseria meningitidis at screening (subjects not providing evidence of prior vaccination should receive vaccination following initiation of administration of the anti-C5-FH fusion protein, but are required to receive treatment with appropriate antibiotic prophylaxis until 2 weeks after vaccination); 9) vaccination against Streptococcus pneumoniae and Hemophilus influenzae (if administration of the anti-C5-FH fusion protein is initiated within 2 weeks of vaccination, appropriate antibiotics should be given for prophylaxis); 10) medical history of asplastic anemia; and 11) medical history of myeloproliferative neoplasms. In some embodiments, the human individual is selected based on body weight. In some embodiments, the human individual having a body weight of less than 80 kg (e.g., about 45 kg to about 79 kg) is selected. In some embodiments, the human individual having a body weight of 80 kg or greater (e.g., about 80 kg to about 120 kg) is selected.

[0100] In some embodiments, the method of treating PNH described herein further comprises excluding a human individual not suitable for such treatment. In some embodiments, a human 35sf-6711731Attorney Docket No.792252001440 individual not suitable for the method of treatment described herein meets one or more of the criteria: 1) any clinically significant poorly controlled underlying illness other than PNH; 2) treatment of any infection with IV (within 30 days of Screening) or oral (within 14 days of Screening) antibiotics, antivirals, or antifungals; 3) history of meningococcal infection; 4) history of untreated tuberculosis; 5) history of splenectomy; 6) positive serology for Hepatitis C Virus (HCV) ribonucleic acid (RNA) or human immunodeficiency virus (HIV) at Screening; 7) history of bone marrow or stem cell transplantation; 8) absolute neutrophilcount (ANC) <500 cells / L; 9) reticulocyte count< 100 × 103 cells / L; 10) platelet count<30,000 cells / L; 11) history of systemic autoimmune disease; 12) estimated glomerularfiltration rate (eGFR) <30 mL / min / 1.73 m2calculated by Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation; 13) known allergy to penicillin antibiotics; 14) history of drug or alcohol abuse within 1 year of Screening; 15) received any type of live attenuated vaccine < 1 month prior to Screening or is planning to receive any such live attenuated vaccine during treatment; 16) use of rituximab within the last 3 months or any complement inhibitors prior to screening; 17) use of steroids (topical use is allowed), EPO, iron supplements, folic acid, vitamin B12, androgen, HIF-PHIs within 4 weeks and immunosuppressive agents within 3 months prior to screening (if doses of above drugs could be maintained stable at this period, it is acceptable); 18) history of malignancy, except adequately treated basal cell carcinoma or in situ carcinoma of the uterine cervix; 19) bone marrow failure and / or candidate for bone marrow transplantation; 20) any female who is pregnant or breastfeeding, or any female who is planning to become pregnant during treatment; 21) a QT duration corrected for heart rate by Fridericia's formula (QTcF) >450 millisecond (msec) for males and > 470 msec for females based on either single or averaged QTcF values of triplicate ECGs obtained over a 3-minute interval; and 22) previously treated with a complement inhibitor. Low Dose Dosing Regimen

[0101] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, and wherein the fusion protein is administered at a maintenance 36sf-6711731Attorney Docket No.792252001440 dose of about 200 mg to about 720 mg (e.g., about 270 mg to about 720 mg) at the maintenance phase. In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase followed by a maintenance phase, wherein the initial phase comprises administering the fusion protein for one or more (e.g., one) initial doses, and the maintenance phase comprises administering the fusion protein (e.g., weekly or biweekly) for two or more (e.g., 2, 3, 4, 5, 6, 8, 10, 11, 12, 13, 14, 23, 24, 25, 26, 40, 44, 47, 48, 49, 50, or more) maintenance doses, wherein the maintenance dose is about 200 mg to about 720 mg (e.g., about 270 mg to about 720 mg). In some embodiments, the complement-mediated disease is selected from the group consisting of PNH, C3G, IgAN, SLE-TMA, and LN-TMA. In some embodiments, the initial dose is about 600 mg to about 1800 mg, such as any of about 1200 mg to about 1800 mg, about 600 mg to about 1200 mg, about 720 mg to about 1440 mg, about 600 mg to about 1000 mg, or about 1200 mg. In some embodiments, the one or more (e.g., one) initial doses of the fusion protein are administered IV. In some embodiments, the initial phase comprises administering the fusion protein at 1, 2, 3, 4, 5, 6, 10, 12, 14, 16, or more initial doses. In some embodiments, the initial phase comprises (or consists essentially of, or consists of) administering a single initial dose of the fusion protein. In some embodiments, the one or more maintenance doses are about 200 mg to about 720 mg, such as any of about 200 mg to about 250 mg, about 270 mg to about 300 mg, about 270 mg to about 450 mg, about 300 mg to about 540 mg, about 300 mg to about 360 mg, or about 400 mg to about 720. In some embodiments, the maintenance dose is about any of 200 mg, 270 mg, 300 mg, 360 mg, 420 mg, 450 mg, 540 mg, 600 mg, or 720 mg. The maintenance dose can be administered IV or SC. In some embodiments, the two or more maintenance doses of the fusion protein are administered weekly. In some embodiments, the two or more maintenance doses of the fusion protein are each administered (e.g., weekly) at about 200 mg SC to about 720 mg SC, such as any of about 300 mg SC to about 540 mg SC, about 270 mg SC, about 300 mg SC, about 420 mg SC, about 540 mg SC, or about 720 mg SC. In some embodiments, the two or more maintenance doses of the fusion protein are administered biweekly. In some embodiments, the two or more maintenance doses of the fusion protein are each administered (e.g., biweekly) at about 270 mg IV to about 450 mg IV, about 300 mg IV to about 500 mg 37sf-6711731Attorney Docket No.792252001440 IV, about 450 mg IV to about 720 mg IV, about 650 mg IV to about 720 mg IV, about 270 mg IV, about 360 mg IV, or about 450 mg IV. In some embodiments, the maintenance phase is at least about 2 weeks, such as at least about any of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 34, 36, 38, 40, 42, 44, 45, 46, 47, 48, 49, 50, 52 weeks, or longer. In some embodiments, the maintenance phase is at least about 12 weeks, such as at least about any of 12 weeks, 13 weeks, 24 weeks, 25 weeks, 48 weeks, or 49 weeks. In some embodiments, the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg weekly for one or more (e.g., one) doses, and wherein the maintenance phase comprises administering the fusion protein at a maintenance dose of about 200 mg to about 720 mg (e.g., about 270 mg to about 720 mg) weekly or biweekly for at least two (e.g., at least any of 4, 5, 12, 13, 24, 25, 48, or 49) doses. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL- CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is a full- length antibody comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration. 38sf-6711731Attorney Docket No.792252001440

[0102] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, wherein the method comprises administering the fusion protein at a) a maintenance dose of about 270 mg SC to about 720 mg SC (e.g., about any of 300 mg SC, 420 mg SC, or 540 mg SC) weekly (QW) (e.g., at least about 12 weeks) for the maintenance phase, or b) a maintenance dose of about 270 mg IV to about 720 mg IV (e.g., about any of 270 mg IV, 360 mg IV, or 450 mg IV) biweekly (Q2W) (e.g., at least about 13 weeks) for the maintenance phase. In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH- IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, wherein the method comprises administering the fusion protein at a single initial dose of about 600 mg IV to about 1800 mg IV (e.g., about any of 600 mg IV, 720 mg IV, 1200 mg IV, 1440 mg IV, or 1800 mg IV) for the initial phase, and administering the fusion protein at a) a maintenance dose of about 270 mg SC to about 720 mg SC (e.g., about any of 300 mg SC, 420 mg SC, or 540 mg SC) weekly (QW) starting Day 8 for at least about 12 weeks for the maintenance phase, or b) a maintenance dose of about 270 mg IV to about 720 mg IV (e.g., about any of 270 mg IV, 360 mg IV, or 450 mg IV) biweekly (Q2W) starting Day 8 for at least about 13 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH- CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is a full-length antibody comprising i) a heavy chain comprising the amino 39sf-6711731Attorney Docket No.792252001440 acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, there is provided a method of treating a complement- mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second FH functional fragment fused to the C- terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, wherein the method comprises administering the fusion protein at a single initial dose of about 600 mg IV to about 1800 mg IV (e.g., about any of 600 mg IV, 720 mg IV, 1200 mg IV, 1440 mg IV, or 1800 mg IV) for the initial phase, and administering the fusion protein at a) a maintenance dose of about 270 mg SC to about 720 mg SC (e.g., about any of 300 mg SC, 420 mg SC, or 540 mg SC) weekly (QW) starting Day 8 for at least about 12 weeks for the maintenance phase, or b) a maintenance dose of about 270 mg IV to about 720 mg IV (e.g., about any of 270 mg IV, 360 mg IV, or 450 mg IV) biweekly (Q2W) starting Day 8 for at least about 13 weeks for the maintenance phase; wherein each heavy chain of the anti-C5 full-length antibody comprises the amino acid sequence of SEQ ID NO: 120, and each light chain of the anti-C5 full-length antibody comprises the amino acid sequence of SEQ ID NO: 90; and wherein each heavy chain fused to the FH functional fragment comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration. 40sf-6711731Attorney Docket No.792252001440

[0103] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 1200 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 300 mg SC weekly for at least about 12 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and 41sf-6711731Attorney Docket No.792252001440 polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0104] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 1200 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 420 mg SC weekly for at least about 12 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID 42sf-6711731Attorney Docket No.792252001440 NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0105] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 1200 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 540 mg SC weekly for at least about 12 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the 43sf-6711731Attorney Docket No.792252001440 amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0106] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 1200 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 270 mg IV biweekly for at least about 13 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL- CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) 44sf-6711731Attorney Docket No.792252001440 a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L- Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0107] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 1200 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 360 mg IV biweekly for at least about 13 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL- 45sf-6711731Attorney Docket No.792252001440 CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L- Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration.

[0108] In some embodiments, there is provided a method of treating a complement-mediated disease (e.g., IgAN) in a human individual, comprising administering to the human individual an effective amount of a fusion protein (e.g., FMEH-IgG4-PLA-FH) comprising i) an antibody moiety that specifically binds to C5 (e.g., human C5; “anti-C5 antibody moiety”) and ii) an FH or functional fragment thereof (e.g., FH SCR domains 1-5, such as SEQ ID NO: 85), wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase; wherein the method comprises administering the fusion protein at a single initial dose of about 1200 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 450 mg IV biweekly for at least about 13 46sf-6711731Attorney Docket No.792252001440 weeks for the maintenance phase. In some embodiments, the anti-C5 antibody moiety comprises a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL- CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody moiety is an anti-C5 full-length antibody, such as comprising i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and ii) a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof, and a second FH or functional fragment thereof. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the fusion protein comprises a first FH functional fragment fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and a second FH functional fragment fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody, wherein the anti-C5 full-length antibody comprises two light chains each comprising the amino acid sequence of SEQ ID NO: 90, and wherein the heavy chain fused to the FH functional fragment each comprises the amino acid sequence of SEQ ID NO: 89. In some embodiments, the maintenance phase is at least about 48 weeks. In some embodiments, the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L- Lys-HCL, and polysorbate 80, pH of about 6.0. In some embodiments, for IV administration, the anti-C5-FH fusion protein is diluted in an infusion bag containing 100 mL of 0.9% sodium chloride for infusion. In some embodiments, for SC administration, the anti-C5-FH fusion protein is administered in naked form and administered into the abdominal wall using standard techniques for SC administration. IgAN

[0109] IgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide. Aberrant glycosylation of IgA1 results in increased serum levels of galactose-deficient IgA1 47sf-6711731Attorney Docket No.792252001440 (Gd-IgA1) that are recognized by glycan-specific IgA and IgG autoantibodies. Aggregates of the immune complexes are formed in situ and / or deposited in the glomerular mesangium. This promotes proliferation of mesangial cells, increased synthesis of extracellular matrix proteins, cytokines, chemokines, and infiltration of immune cells into the surrounding tissue. Accordingly, disease progression involves (1) production of Gd-IgA1; and (2) its recognition by antiglycan autoantibodies; which (3) form immune complexes in the kidney; and (4) activate mesangial cells. See, e.g., Penfold et al., Int. J. Nephrol. and Renovascular Dis.11, pp.137-148 (2017).

[0110] IgAN occurs primarily in subjects in their 20s and 30s. Patients present with a range of symptoms, typically including micro- or macro-hematuria and increased protein excretion in the urine. Patients may also present with hypertension as a result of sustained renal damage. Current therapeutic approaches merely provide supportive care, including administration of the maximum tolerable dose of an angiotensin converting enzyme inhibitor or angiotensin-receptor blocker, or administration of immunosuppressive drugs, whose benefits are largely outweighed by adverse reactions. Ultimately, 30-40% of patients will develop end-stage renal disease (ESRD) within 20-30 years of diagnosis of IgAN. In the interim, patients experience numerous symptoms that significantly degrade their quality of life, in addition to declining renal function. Patients with IgAN often exhibit significantly increased expressions of endothelin 1 (ET-1) and ET-RA in the kidney. Increased expression of endothelins positively correlates with proteinuria, one of the hallmark symptoms of IgAN.

[0111] In some embodiments, the method of treating IgAN described herein can prevent (e.g., preventing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% chance), delay (e.g., delaying at least about any of 1, 2, 3, 6, 12, 18, 24, 30, 36, 42, 48, 52 months or longer), or reduce (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) the severity of one or more of IgAN signs and symptoms discussed above, including but not limited to, proteinuria, hematuria, pain in back, edema, high blood pressure, as well as complications like high cholesterol, acute kidney failure, chronic kidney failure, nephrotic syndrome. In some embodiments, the method of treating IgAN described herein can achieve one or more of following effects: i) decreasing (e.g., decreasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) renal inflammation and / or fibrosis; ii) reducing (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) production of Gd-IgA1; iii) decreasing (e.g., decreasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) the occurrence of hematuria; iv) stabilizing (e.g., not varying more than 48sf-6711731Attorney Docket No.792252001440 about 30%, 20%, 10%, 5% or less) eGFR; v) delaying (e.g., delaying at least about any of 1, 2, 3, 6, 12, 18, 24, 30, 36, 42, 48, 52 months or longer) the onset of ESRD; vi) decreasing (e.g., decreasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) the number of IgA-nephropathy associated disease flares; vii) decreasing (e.g., decreasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) proteinuria; viii) decreasing (e.g., decreasing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) fatigue; ix) inhibiting (e.g., inhibiting at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) mesangial cell activation; x) reducing (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) activation of a mesangial cell in contact with an IgA immune complex; xi) reducing (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) expressions of endothelin 1 (ET-1) and ET-RA in the kidney; and xii) improving (e.g., improving at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) renal function and / or renal pathology score. In some embodiments, the method of treating IgAN described herein can reduce (e.g., reducing at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) one or more of: C3 deposit score, C3b activity, free serum C5 level, eGFR, UPCR, and RBC lysis. In some embodiments, the method of treating IgAN described herein can improve (e.g., improving at least about any of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) quality of life, such as assessed by the FACIT-Fatigue Score and KDQoL scale, and / or histology and histopathology of renal biopsy.

[0112] In some embodiments, the efficacy of the method of treating IgAN described herein can be assessed by one or more of: i) the percent change from baseline in 24-hour UPCR; ii) change from baseline in Rabbit RBC assay, change from baseline in C3b activity assay, change from baseline in serum and urine Factor H level, change from baseline in urine MCP- 1, C3a, C5a, properdin, or C5b-9 level, and / or change from baseline in free serum C5 levels; iii) change in eGFR; iv) change in quality of life assessed by the FACIT-Fatigue Score and KDQoL scale; and v) change in histology and histopathology in subjects undergoing repeat renal biopsy. Such methods are known in the art. In some embodiments, changes from baseline in markers of alternative complement pathway involvement, e.g., C3, C3d, C3c, C3adesArg, C5, C5a, C5b-9, C5adesArg, and other markers of inflammation, may be assessed in plasma / serum or urine over the course of the treatment period.

[0113] In some embodiments, the method of treating IgAN described herein further comprises selecting a human individual (e.g., complement inhibitor-naïve human individual) 49sf-6711731Attorney Docket No.792252001440 suitable for such treatment. In some embodiments, the human individual to be treated has prior vaccination against one or all of Neisseria meningitidis, Streptococcus pneumoniae, and Hemophilus influenzae. In some embodiments, the human individual further receives antibiotic prophylaxis during the treatment. In some embodiments, individuals not vaccinated with above vaccination will receive prophylactic antibiotics starting no later than concurrently with the first dose of the anti-C5-FH fusion protein, and will continue to receive this antibiotic prophylaxis treatment until 2 weeks after vaccination. In some embodiments, the human individual is between ages of about 18 years to about 75 years. In some embodiments, the human individual meets one or more of the criteria: 1) weight of >35 kilograms (kg) at Screening; 2) body mass index (BMI) of <35 kilograms per square meter (kg / m2); 3) UPCR >1.5 grams per gram (g / g) by 24-hour urine collection at Screening; 4) documented diagnosis and clinical status of IgAN; 5) tested for negative pregnancy, and effective contraception during entire treatment period; 6) vaccination; and 7) able to provide informed consent. In some embodiments, IgAN is verified by biopsy in the human individual to be treated. In some embodiments, the human individual is on stable regimen of angiotensin converting enzyme or angiotensin blocking agents for 12 weeks and / or sodium-glucose cotransporter-2 (SGLT2) inhibitors for 6 weeks at Screening.

[0114] In some embodiments, the method of treating IgAN described herein further comprises excluding a human individual not suitable for such treatment. In some embodiments, a human individual not suitable for the method of treatment described herein meets one or more of the criteria: 1) any clinically significant, poorly controlled underlying illness other than IgAN; 2) any history or sign of significant chronic active or recurrent infection, such as those requiring treatment or being treated with antibiotics, antivirals, or antifungals; 3) history of infections with encapsulated organisms; 4) history of untreated tuberculosis; 5) known allergy to penicillin antibiotics; 6) known or suspected immunodeficiency disease, including hereditary complement deficiency; 7) positive serology for hepatitis C virus (HCV) ribonucleic acid (RNA) or human immunodeficiency virus (HIV) at Screening; 8) history of bone marrow or stem cell transplantation; 9) absolute neutrophilcount (ANC) <500 cells per microliter (cells / L); 10) eGFR <30 milliliters per minute per1.73 square meter (mL / min / 1.73 m2) using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula; 11) presence of crescent formation in >50 percent (%) of glomeruli assessed on renal biopsy; 12) nephrotic syndrome; 13) rapidly progressive glomerulonephritis, defined as a fall in eGFR of > 30 mL / min / 1.73 m2within 24 weeks prior to the Screening Visit; 14) receiving renal replacement therapy or anticipated to require renal 50sf-6711731Attorney Docket No.792252001440 replacement therapy during the duration of the treatment; 15) history of drug or alcohol abuse within 1 year of screening; 16) received any type of live attenuated vaccine <4 weeks prior to screening or is planning to receive any such live attenuated vaccine over the course of the treatment; 17) use of rituximab within the last 12 weeks or any prior use of complement inhibitors; 18) use of systemic corticosteroids >10 mg per day (prednisone or equivalent) within 4 weeks prior to screening or immunosuppressive agents (e.g., mycophenolate mofetil, hydroxychloroquine, cyclosporin etc.) within 3 months prior to screening; 19) history of malignancy, except adequately treated basal cell carcinoma or in situ carcinoma of the uterine cervix; 20) any female who is pregnant or breastfeeding, or any female who is planning to become pregnant during the treatment; 21) a QT duration corrected for heart rate by Fridericia’s formula (QTcF) >450 millisecond (msec) for males and >470 msec for females based on either single or averaged QTcF values of triplicate ECGs obtained over a 3-minute interval; 22) diagnosed with secondary forms of IgAN (e.g., Henoch Schönlein purpura, IgA vasculitis); and 23) use of any complement inhibitors.

[0115] The anti-C5-FH fusion proteins and components thereof are described in more details below. Anti-C5-fH Fusion Protein

[0116] Any anti-C5-FH fusion proteins and anti-C5 antibody moieties (e.g., pH-dependent anti-C5 antibody moieties) described in US20220204602, US20220177556, US11578137, WO2020219922, and WO2024097796 can be used herein, the content of each of which is incorporated herein by reference in their entirety.

[0117] The methods descried herein utilize a fusion protein comprising an anti-C5 antibody moiety (such as any one of the anti-C5 antibodies or antigen-binding fragments thereof described herein) and an FH or a functional fragment thereof. In some embodiments, the anti- C5 antibody moiety is a full-length antibody (hereinafter referred to as “anti-C5 full-length antibody”). In some embodiments, the FH or functional fragment thereof is fused to one or both of the heavy chains of the anti-C5 full-length antibody (for example at the C-terminal end of the heavy chain(s)). In some embodiments, the anti-C5 full-length antibody comprises an Fc fragment derived from human IgG4 (such as an IgG4 Fc fragment comprising an PLA mutation (S228P / M428L / N434A)). In some embodiments, the IgG4 Fc fragment comprises the amino acid sequence of any of SEQ ID NOs: 32, 33, and 61. In some embodiments, the IgG4 Fc fragment comprising an PLA mutation comprises an amino acid sequence set forth in SEQ ID NO: 61. In some embodiments, the fragment of FH inhibits C3 activation. In some 51sf-6711731Attorney Docket No.792252001440 embodiments, the functional fragment of FH comprises short consensus repeat (SCR) domains 1-5 of FH. In some embodiments, the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 85. In some embodiments, the nucleic acid that encodes an IgG4 Fc comprising the amino acid sequence of SEQ ID NO: 61 comprises the nucleic acid sequence of SEQ ID NO: 60.

[0118] In some embodiments, the fusion protein comprises an anti-C5 antibody moiety (such as any one of the anti-C5 antibodies or antigen-binding fragments thereof described herein) and an FH or a functional fragment thereof comprising SCR1-5 domains of FH. In some embodiments, the fusion protein comprises i) an anti-C5 antibody moiety (such as any one of the anti-C5 antibodies described herein) comprising an IgG4 Fc fragment (e.g., an IgG4 Fc fragment comprising a PLA mutation), and ii) an FH or a functional fragment thereof comprising SCR1-5 domains of FH. In some embodiments, the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 85. In some embodiments, the IgG4 Fc fragment comprises the amino acid sequence of SEQ ID NO: 61.

[0119] In some embodiments, the fusion protein comprises an anti-C5 antibody moiety and an FH or a functional fragment thereof, wherein the anti-C5 antibody moiety is pH-sensitive or pH-dependent (such as any one of the pH-sensitive or pH-dependent anti-C5 antibodies or antigen-binding fragments thereof described herein). For example, in some embodiments, the pH-sensitive anti-C5 antibody moiety is an anti-C5 antibody moiety that binds to C5 at a higher affinity at a neutral pH (e.g., about pH 7.4) than at an acidic pH (e.g., about pH 5.8). In some embodiments, the binding affinity of the pH-dependent anti-C5 antibody moiety to C5 (e.g., human C5) at about pH 7.4 is at least about 3 times (such as at least about any of 4, 5, 6, 7, 8, 9, 10, or more) times higher than the binding affinity of the pH-dependent anti-C5 antibody moiety to C5 (e.g., human C5) at about pH 5.8. In some embodiments, the pH- dependent anti-C5 antibody moiety comprises an Fc fragment derived from human IgG4 (e.g., an IgG4 Fc fragment comprising a PLA mutation). In some embodiments, the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 85. In some embodiments, the IgG4 Fc fragment comprises the amino acid sequence of SEQ ID NO: 61.

[0120] In some embodiments, the fusion protein comprises an anti-C5 antibody moiety and an FH or functional fragment thereof, wherein the anti-C5 antibody moiety is pH-sensitive or pH-dependent (such as any one of the pH-sensitive or pH-dependent anti-C5 antibodies described herein), for example binding to C5 (e.g., human C5) at a higher affinity at pH 7.4 than at pH 5.8, wherein the anti-C5 antibody moiety comprising an IgG4 Fc fragment (e.g., an IgG4 Fc fragment comprising a PLA mutation). In some embodiments, the fusion protein 52sf-6711731Attorney Docket No.792252001440 comprises an anti-C5 antibody moiety and an FH or functional fragment thereof, wherein the anti-C5 antibody moiety is pH-sensitive or pH-dependent (such as any one of the pH- sensitive or pH-dependent anti-C5 antibodies or antigen-binding fragments thereof described herein), for example binding to C5 (e.g., human C5) at a higher affinity at pH 7.4 than at pH 5.8, and wherein the FH or functional fragment thereof comprises SCR1-5 domains of FH. In some embodiments, the fusion protein comprises an anti-C5 antibody moiety and an FH or functional fragment thereof, wherein the anti-C5 antibody moiety is pH-sensitive or pH- dependent (such as any one of the pH-sensitive or pH-dependent anti-C5 antibodies described herein), for example binding to C5 (e.g., human C5) at a higher affinity at pH 7.4 than at pH 5.8, wherein the anti-C5 antibody moiety comprises an IgG4 Fc fragment (e.g., an IgG4 Fc fragment comprising a PLA mutation), and wherein the FH or functional fragment thereof comprises SCR1-5 domains of FH. In some embodiments, the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 85. In some embodiments, the IgG4 Fc fragment comprises the amino acid sequence of SEQ ID NO: 61.

[0121] In some embodiments, the fusion protein comprises an anti-C5 antibody moiety (such as any one of the anti-C5 antibodies or antigen-binding fragments thereof described herein) and an FH or functional fragment thereof, wherein the anti-C5 antibody moiety comprises a histidine substitution in one or more of its CDR regions (such as any one of the histidine- containing anti-C5 antibodies or antigen-binding fragments thereof described herein). In some embodiments, the fusion protein comprises an anti-C5 antibody moiety and an FH or functional fragment thereof, wherein the anti-C5 antibody moiety comprises a histidine substitution in one or more of its CDR regions (such as any one of the histidine-containing anti-C5 antibodies described herein), and wherein the anti-C5 antibody moiety comprises an IgG4 Fc fragment (e.g., an IgG4 Fc fragment comprising a PLA mutation). In some embodiments, the fusion protein comprises an anti-C5 antibody moiety and an FH or functional fragment thereof, wherein the anti-C5 antibody moiety comprises a histidine substitution in one or more of its CDR regions (such as any one of the histidine-containing anti-C5 antibodies or antigen-binding fragments thereof described herein), and wherein the FH or functional fragment thereof comprises SCR1-5 domains of FH. In some embodiments, the fusion protein comprises an anti-C5 antibody moiety and an FH or functional fragment thereof, wherein the anti-C5 moiety comprises a histidine substitution in one or more of its CDR regions (such as any one of the histidine-containing anti-C5 antibodies described herein), wherein the anti-C5 antibody moiety comprises an IgG4 Fc fragment (e.g., an IgG4 Fc fragment comprising a PLA mutation), and wherein the FH or functional fragment thereof 53sf-6711731Attorney Docket No.792252001440 comprises SCR1-5 domains of FH. In some embodiments, the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 85. In some embodiments, the IgG4 Fc fragment comprises the amino acid sequence of SEQ ID NO: 61.

[0122] In some embodiments, the FH or functional fragment thereof (e.g., SEQ ID NO: 85) is fused to the anti-C5 antibody moiety (e.g., any one of the anti-C5 antibodies or antigen- binding fragments thereof described herein) via a linker (e.g., peptide linker), such as fused to the C-terminus of the anti-C5 antibody moiety via a linker. Any suitable peptide linker can be used herein, including but not limited to, a GS linker. In some embodiments, the FH or functional fragment thereof (e.g., SEQ ID NO: 85) is directly fused to the anti-C5 antibody moiety (e.g., any one of the anti-C5 antibodies or antigen-binding fragments thereof described herein), such as directly fused to the C-terminus of the anti-C5 antibody moiety. In some embodiments, the anti-C5 antibody moiety is a full-length antibody. In some embodiments, the FH or functional fragment thereof is fused to the C-terminus of one or both heavy chains of the anti-C5 full-length antibody, either directly or via a peptide linker, such as fusing directly. In some embodiments, the fusion protein comprises a first FH or functional fragment thereof and a second FH or functional fragment thereof, wherein the first FH or functional fragment thereof is fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and the second FH or functional fragment thereof is fused to the C- terminus of a second heavy chain of the anti-C5 full-length antibody.

[0123] In some embodiments, the fusion protein further comprises a signal peptide at the N- terminus of one or more of its polypeptide chain(s). Any suitable signal peptide for protein / polypeptide export or expression can be used herein, including but not limited to SEQ ID NO: 91 or 92. In some embodiments, the anti-C5 antibody moiety is a full-length antibody, the FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody, and the fusion protein comprises one or more signal peptides fused to the N-terminus of one or both VHs or heavy chains, and / or one or both VLs or light chains (e.g., both heavy chains and both light chains) of the anti-C5 full-length antibody. In some embodiments, the signal peptide fused to the VH(s) or the heavy chain(s) of the anti-C5 antibody or antigen-binding fragment thereof comprises the amino acid sequence of SEQ ID NO: 91. In some embodiments, the signal peptide fused to the VL(s) or the light chain(s) of the anti-C5 antibody or antigen-binding fragment thereof comprises the amino acid sequence of SEQ ID NO: 92. In some embodiments, the signal peptide is cleaved in the mature or secreted fusion protein format. In some embodiments, the fusion protein does not comprise any signal peptide. 54sf-6711731Attorney Docket No.792252001440

[0124] In some embodiments, there is provided a fusion protein comprising an anti-C5 antibody moiety (e.g., anti-C5 full-length antibody) and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), wherein the anti-C5 antibody moiety comprises: (i) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 11; (ii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (iii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (iv) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 20, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (v) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 26, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 29; (vi) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 34, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (vii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 37, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 38, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39, a VL-CDR1 comprising the amino acid 55sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (viii) a VH- CDR1 comprising the amino acid sequence of SEQ ID NO: 42, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 43, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (ix) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 48, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (x) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 52, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 53, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (xi) a VH- CDR1 comprising the amino acid sequence of SEQ ID NO: 47, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (xii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xiii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 37, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 62, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (xiv) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 42, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 65, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 56sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 10; (xv) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 52, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 68, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54, a VL- CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (xvi) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; (xvii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xviii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 97; (xix) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xx) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxi) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 102, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, 57sf-6711731Attorney Docket No.792252001440 a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 26, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 105; (xxiii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 34, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxiv) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 38, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxv) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 43, a VH- CDR3 comprising the amino acid sequence of SEQ ID NO: 44, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxvi) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 48, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL- CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxvii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 53, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxviii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 62, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; (xxix) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 65, a VH-CDR3 58sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 44, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96; or (xxx) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 68, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL- CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, the anti-C5 antibody moiety comprises: (i) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 98; (ii) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 99; (iii) a VH comprising the amino acid sequence of SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95; (iv) a VH comprising the amino acid sequence of SEQ ID NO: 103, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (v) a VH comprising the amino acid sequence of SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106; (vi) a VH comprising the amino acid sequence of SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95; (vii) a VH comprising the amino acid sequence of SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (viii) a VH comprising the amino acid sequence of SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (ix) a VH comprising the amino acid sequence of SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (x) a VH comprising the amino acid sequence of SEQ ID NO: 112, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (xi) a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (xii) a VH comprising the amino acid sequence of SEQ ID NO: 113, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (xiii) a VH comprising the amino acid sequence of SEQ ID NO: 114, and a VL comprising the amino acid sequence of SEQ ID NO: 88; (xiv) a VH comprising the amino acid sequence of SEQ ID NO: 115, and a VL comprising the amino acid sequence of SEQ ID NO: 88; or (xv) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, there is provided a fusion protein comprising an anti-C5 antibody moiety (e.g., anti-C5 full-length antibody) and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), wherein the anti-C5 antibody moiety comprises: (i) a VH-CDR1 59sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 47, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; or (ii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, there is provided a fusion protein comprising an anti- C5 antibody moiety (e.g., anti-C5 full-length antibody) and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), wherein the anti-C5 antibody moiety comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85) is fused to the C-terminus of the anti-C5 antibody moiety. In some embodiments, the anti-C5 antibody moiety is a full-length antibody. In some embodiments, the anti-C5 full-length antibody comprises an Fc fragment derived from human IgG4, such as an Fc fragment comprising the amino acid sequence of any of SEQ ID NOs: 32, 33, and 61, e.g., SEQ ID NO: 61. In some embodiments, the FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85) is fused to (e.g., direct fusion) the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full-length antibody. In some embodiments, the anti- C5 full-length antibody comprises a heavy chain comprising the amino acid sequence of any of SEQ ID NOs: 120, 122, 124, and 126, and a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, the anti-C5 full-length antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and a light chain comprising the amino acid sequence of SEQ ID NO: 90.

[0125] In some embodiments, there is provided a fusion protein comprising an anti-C5 full- length antibody and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85); wherein the FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of one or both (e.g., both) of the heavy chains of the anti-C5 full- length antibody; and wherein the anti-C5 full-length antibody comprises: (i) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ 60sf-6711731Attorney Docket No.792252001440 ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10; or (ii) a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96. In some embodiments, there is provided a fusion protein comprising an anti- C5 full-length antibody and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85); wherein the FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of one or both (e.g., both) of the heavy chains of the anti-C5 full-length antibody; and wherein the anti-C5 full-length antibody comprises a VH comprising the amino acid sequence of SEQ ID NO: 87, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 full-length antibody comprises an Fc fragment comprising the amino acid sequence of SEQ ID NO: 61. In some embodiments, there is provided a fusion protein comprising an anti-C5 full-length antibody and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85); wherein the FH or functional fragment thereof is fused to (e.g., direct fusion) the C- terminus of one or both (e.g., both) of the heavy chains of the anti-C5 full-length antibody; and wherein the anti-C5 full-length antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 120, and a light chain comprising the amino acid sequence of SEQ ID NO: 90.

[0126] In some embodiments, the anti-C5 antibody moiety is a full-length antibody. In some embodiments, the anti-C5 full-length antibody comprises an Fc fragment comprising the amino acid sequence of SEQ ID NO: 61. In some embodiments, the FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85) is fused to (e.g., direct fusion) the C-terminus of one or both (e.g., both) heavy chains of the anti-C5 full- length antibody. In some embodiments, there is provided a fusion protein comprising an anti- C5 full-length antibody (such as any of the anti-C5 full-length antibodies described herein) and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85); wherein the FH or functional fragment thereof is fused to (e.g., direct fusion) the C- terminus of one of the heavy chains of the anti-C5 full-length antibody. In some embodiments, there is provided a fusion protein comprising an anti-C5 full-length antibody and an FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 61sf-6711731Attorney Docket No.792252001440 85); wherein the FH or functional fragment thereof is fused to (e.g., direct fusion) the C- terminus of one of the heavy chains of the anti-C5 full-length antibody; and wherein the anti- C5 full-length antibody comprises a heavy chain comprising the amino acid sequence of any of SEQ ID NOs: 120, 122, 124, and 126, and a light chain comprising the amino acid sequence of SEQ ID NO: 90. In some embodiments, there is provided a fusion protein comprising a first FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), a second FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), and an anti-C5 full-length antibody (such as any of the anti-C5 full- length antibodies described herein); wherein the first FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and the second FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of a second heavy chain of the anti-C5 full-length antibody. In some embodiments, there is provided a fusion protein comprising a first FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), a second FH or functional fragment thereof (e.g., SCR1-5 domains of FH, such as SEQ ID NO: 85), and an anti-C5 full- length antibody; wherein the first FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and the second FH or functional fragment thereof is fused to (e.g., direct fusion) the C-terminus of a second heavy chain of the anti-C5 full-length antibody; and wherein the anti-C5 full-length antibody comprises a heavy chain comprising the amino acid sequence of any of SEQ ID NOs: 120, 122, 124, and 126, and a light chain comprising the amino acid sequence of SEQ ID NO: 90.

[0127] In some embodiments, there is provided a fusion protein comprising i) an anti-C5 full- length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti- C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 120, and two light chains each comprising the amino acid sequence of SEQ ID NO: 90; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 89. This fusion protein is hereinafter referred to as “FMEH-IgG4-PLA-FH”. In some embodiments, the fusion protein further comprises a signal peptide (e.g., SEQ ID NO: 91 or 92) at the N-terminus of one or both heavy chains and / or one or both light chains of the anti-C5 full-length antibody, such as both heavy chains and both light chains of the anti-C5 full-length antibody. In some embodiments, 62sf-6711731Attorney Docket No.792252001440 there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full- length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 119, and two light chains each comprising the amino acid sequence of SEQ ID NO: 74; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 72.

[0128] In some embodiments,, there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 122, and two light chains each comprising the amino acid sequence of SEQ ID NO: 90; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 116. In some embodiments, the fusion protein further comprises a signal peptide (e.g., SEQ ID NO: 91 or 92) at the N-terminus of one or both heavy chains and / or one or both light chains of the anti-C5 full-length antibody, such as both heavy chains and both light chains of the anti-C5 full-length antibody. In some embodiments, there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 121, and two light chains each comprising the amino acid sequence of SEQ ID NO: 74; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 76.

[0129] In some embodiments,, there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 124, and two light chains each comprising the amino acid sequence of SEQ ID NO: 90; and wherein each heavy chain fused to the functional fragment of FH 63sf-6711731Attorney Docket No.792252001440 comprises the amino acid sequence of SEQ ID NO: 117. In some embodiments, the fusion protein further comprises a signal peptide (e.g., SEQ ID NO: 91 or 92) at the N-terminus of one or both heavy chains and / or one or both light chains of the anti-C5 full-length antibody, such as both heavy chains and both light chains of the anti-C5 full-length antibody. In some embodiments, there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 123, and two light chains each comprising the amino acid sequence of SEQ ID NO: 74; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 78.

[0130] In some embodiments,, there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 126, and two light chains each comprising the amino acid sequence of SEQ ID NO: 90; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 118. In some embodiments, the fusion protein further comprises a signal peptide (e.g., SEQ ID NO: 91 or 92) at the N-terminus of one or both heavy chains and / or one or both light chains of the anti-C5 full-length antibody, such as both heavy chains and both light chains of the anti-C5 full-length antibody. In some embodiments, there is provided a fusion protein comprising i) an anti-C5 full-length antibody, ii) a first functional fragment of FH fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and iii) a second functional fragment of FH fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody; wherein the anti-C5 full-length antibody comprises two heavy chains each comprising the amino acid sequence of SEQ ID NO: 125, and two light chains each comprising the amino acid sequence of SEQ ID NO: 74; and wherein each heavy chain fused to the functional fragment of FH comprises the amino acid sequence of SEQ ID NO: 80. Anti-C5 Antibody Moiety 64sf-6711731Attorney Docket No.792252001440

[0131] The fusion protein descried herein comprises an antibody moiety that specifically binds to C5. In some embodiments, the anti-C5 antibody moiety is a chimeric antibody. In further embodiments, the anti-C5 antibody moiety is a humanized antibody. In some embodiments, the anti-C5 antibody moiety is a full-length antibody, a Fab, a Fab’, a F(ab)2, a F(ab’)2, an scFv, or a combination thereof. In some embodiments, the anti-C5 antibody moiety is an antibody fragment (e.g., Fab, scFv). In some embodiments, the anti-C5 antibody moiety is a full-length antibody, such as a full-length antibody comprising an Fc fragment derived from IgG4 (e.g., an IgG4 Fc comprising a PLA mutation). In some embodiments, the IgG4 Fc fragment comprises the amino acid sequence of any of SEQ ID NOs: 32, 33, and 61, such as SEQ ID NO: 61. In some embodiments, the binding of the anti-C5 antibody moiety to C5 is pH-dependent, and wherein the anti-C5 antibody moiety binds more strongly to C5 at a neutral pH (e.g., about pH 7.4) than it does at an acidic pH (e.g., about pH 5.8). In some embodiments, the C5 is human C5. In some embodiments, an anti-C5 antibody moiety comprises (or consists of, or consists essentially of) an anti-C5 antibody or antigen-binding fragment thereof. In some embodiments, the anti-C5 antibody moiety consists of any of the anti-C5 antibodies or antigen-binding fragments thereof described herein.

[0132] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof binds to an epitope in the -chain of C5, and / or an epitope in the -chain of C5.

[0133] It is to be understood that the anti-C5 antibody moiety described herein can derive from any of the anti-C5 antibodies or antibody fragments thereof discussed below in more detail.

[0134] In some embodiments, the anti-C5 antibody moiety exhibits pH-dependent binding to C5. In some embodiments, the pH-dependent anti-C5 antibody moiety binds more strongly to C5 at a more neutral pH (e.g., about pH 7.4; such as that found in the blood) than it does at a more acidic pH (e.g., about pH 5.8; such as that found in the endosome). In some embodiments, the pH-dependent anti-C5 antibody moiety is a variant (e.g., comprising one or more amino acid substitutions, such as conserved substitution) of humanized anti-C5 antibody comprising a VH comprising the amino acid sequence of SEQ ID NO: 2 and a VL comprising the amino acid sequence of SEQ ID NO: 7.

[0135] In some embodiments, binding of the anti-C5 antibody or fragment of the antibody to human-C5 is associated with a reduction in the generation of C5a or C5b and the formation of MAC in the complement activation pathway in an intact organism. In some embodiments, the anti-C5 antibody moiety is capable of binding to human C5. In some embodiments, the anti- C5 antibody or antibody fragment binds to a relevant portion or fraction or epitope of the 65sf-6711731Attorney Docket No.792252001440 human-C5; and the binding of the anti-C5 antibody or antibody fragment thereof to the relevant portion of the human-C5 is associated with a reduction in the generation of C5a or C5b and the formation of MAC in an intact organism.

[0136] In some embodiments, the anti-C5 antibody or antibody fragment thereof is further conjugated to a protein, a peptide or another compound. In some embodiments, the anti-C5 antibody or antibody fragment thereof, is conjugated to a protein, a peptide, or other compound. In some embodiments, the protein, peptide, or other compound to which the anti- C5 antibody or antibody fragment thereof is conjugated is a targeting moiety (i.e., the targeting moiety specifically binds to a molecule other than human-C5). In some embodiments, the protein, peptide, or other compound to which the anti-C5 antibody or antibody fragment thereof is conjugated to is an effector molecule (e.g., a cytotoxic molecule).

[0137] In various embodiments, any of the anti-C5 antibodies described herein, having any of the variable regions described herein, may comprise an Fc fragment or Fc domain. For example, in some embodiments, an anti-C5 antibody described herein, comprises an Fc fragment of an immunoglobulin. Exemplary immunoglobulins include, but is not limited to, IgG1, IgG2, IgG3, IgG4, IgM, IgA, IgE, and IgD. In some embodiments, the anti-C5 antibody comprises an Fc of human IgG4. SEQ ID NO: 32 is an example amino acid sequence of a human IgG4 Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32. SEQ ID NO: 33 is an exemplary amino acid sequence of a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having one or more of: an S108P mutation, a M308L mutation, and a N314A mutation, relative to SEQ ID NO: 32. In some embodiments, the anti- C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation, relative to SEQ ID NO: 32 (also referred to herein as having an IgG4 Fc “PLA” mutation). SEQ ID NO: 61 is an exemplary amino acid sequence of a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32.

[0138] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 66sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0139] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0140] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0141] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0142] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, or a variant 67sf-6711731Attorney Docket No.792252001440 thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0143] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0144] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 2, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 2, and a VL comprising the amino acid sequence of SEQ ID NO: 7, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 7. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 2, and a VL comprising the amino acid sequence of SEQ ID NO: 7. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 93, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 95, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 95. In some embodiments, the anti-C5 antibody or 68sf-6711731Attorney Docket No.792252001440 antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, the VH and VL do not comprise a signal peptide at the N-terminus.

[0145] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 2, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 2. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 7, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 7. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb 2G1, or a variant thereof (e.g., humanized 2G1). In some embodiments, the anti-C5 antibody mAb 2G1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb 2G1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb 2G1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb 2G1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH 69sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0146] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 11, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, e.g., conserved substitution) in one or more of the CDRs.

[0147] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 11, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0148] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 11. 70sf-6711731Attorney Docket No.792252001440

[0149] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 97, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0150] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 97, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0151] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 97.

[0152] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 2, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 2, and a VL comprising the amino acid sequence of SEQ ID NO: 13, or a variant thereof comprising at least about 80% (e.g., at least 71sf-6711731Attorney Docket No.792252001440 about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 13. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 2, and a VL comprising the amino acid sequence of SEQ ID NO: 13. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 93, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 98, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 98. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 98. In some embodiments, the VH and VL do not comprise a signal peptide at the N-terminus.

[0153] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 2, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 2. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 13, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 13. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb L3-1, or a variant thereof. In some embodiments, the anti-C5 antibody mAb L3-1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 13; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 98. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb L3-1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 13; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 98; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. 72sf-6711731Attorney Docket No.792252001440 In some embodiments, the anti-C5 antibody mAb L3-1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 13; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 98; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb L3-1 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 13; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93 and a VL comprising the amino acid sequence of SEQ ID NO: 98; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0154] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0155] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved 73sf-6711731Attorney Docket No.792252001440 substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0156] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0157] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0158] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0159] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- 74sf-6711731Attorney Docket No.792252001440 CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 14; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0160] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 2, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 2, and a VL comprising the amino acid sequence of SEQ ID NO: 16, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 16. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 2, and a VL comprising the amino acid sequence of SEQ ID NO: 16. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 93, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 99, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 99. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 99. In some embodiments, the VH and VL do not comprise a signal peptide at the N-terminus.

[0161] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 2, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 2. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 16, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 16. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb L1-2, or a variant thereof. In some embodiments, the anti-C5 antibody mAb L1-2 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 16; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL 75sf-6711731Attorney Docket No.792252001440 comprising the amino acid sequence of SEQ ID NO: 99. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb L1-2 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 16; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 99; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb L1-2 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 16; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 99; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb L1-2 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 2 and a light chain comprising the amino acid sequence of SEQ ID NO: 16; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 93, and a VL comprising the amino acid sequence of SEQ ID NO: 99; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0162] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0163] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions 76sf-6711731Attorney Docket No.792252001440 (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0164] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0165] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0166] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising 77sf-6711731Attorney Docket No.792252001440 the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0167] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 17; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0168] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 19, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 19, and a VL comprising the amino acid sequence of SEQ ID NO: 7, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 7. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 19, and a VL comprising the amino acid sequence of SEQ ID NO: 7. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 101, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 95. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0169] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 19, or a variant thereof comprising at least about 80% 78sf-6711731Attorney Docket No.792252001440 (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 19. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 7, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 7. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H1-4, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-4 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H1-4 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-4 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-4 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 19 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 101, and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61. 79sf-6711731Attorney Docket No.792252001440

[0170] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 20; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0171] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 20, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0172] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 20; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0173] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 102; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a 80sf-6711731Attorney Docket No.792252001440 VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0174] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 102, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 4, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0175] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 102; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 4; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0176] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 22, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 22, and a VL comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 22, and a VL comprising the amino acid sequence of SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid 81sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 103, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 103, and a VL comprising the amino acid sequence of SEQ ID NO: 88, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 88. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 103, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0177] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 22, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 22. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H1-8 / L1-9, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 22 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 103, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 22 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 103, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1- 8 / L1-9 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 22 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 103, and a VL comprising the amino 82sf-6711731Attorney Docket No.792252001440 acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 22 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 103, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0178] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 26; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 29, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0179] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 26, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 29, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution). 83sf-6711731Attorney Docket No.792252001440

[0180] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 26; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 29.

[0181] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 26; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 105, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0182] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 26, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 105, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0183] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 26; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of 84sf-6711731Attorney Docket No.792252001440 SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 105.

[0184] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 28, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 28, and a VL comprising the amino acid sequence of SEQ ID NO: 31, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 31. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 28, and a VL comprising the amino acid sequence of SEQ ID NO: 31. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 104, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 106. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0185] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 28, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 28. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 31, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 31. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H2-6 / L3-5, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H2-6 / L3-5 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 28 and a light chain comprising the amino acid sequence of SEQ ID NO: 31; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106. In some 85sf-6711731Attorney Docket No.792252001440 embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H2-5 / L3-5 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 28 and a light chain comprising the amino acid sequence of SEQ ID NO: 31; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H2-6 / L3-5 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 28 and a light chain comprising the amino acid sequence of SEQ ID NO: 31; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H2-61L3-5 comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 28 and a light chain comprising the amino acid sequence of SEQ ID NO: 31; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 104, and a VL comprising the amino acid sequence of SEQ ID NO: 106; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0186] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 34; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0187] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions 86sf-6711731Attorney Docket No.792252001440 (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 34, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0188] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 3; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 34; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0189] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 34; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0190] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 34, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising 87sf-6711731Attorney Docket No.792252001440 the amino acid sequence of SEQ ID NO: 8, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0191] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 94; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 34; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 5; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 8; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0192] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 36, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 36, and a VL comprising the amino acid sequence of SEQ ID NO: 7, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 7. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 36, and a VL comprising the amino acid sequence of SEQ ID NO: 7. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 107, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 95. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0193] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 36, or a variant thereof comprising at least about 80% 88sf-6711731Attorney Docket No.792252001440 (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 36. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 7, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 7. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 36 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 36 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 36 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 36 and a light chain comprising the amino acid sequence of SEQ ID NO: 7; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 107, and a VL comprising the amino acid sequence of SEQ ID NO: 95; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0194] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 37; a VH- 89sf-6711731Attorney Docket No.792252001440 CDR2 comprising the amino acid sequence of SEQ ID NO: 38; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0195] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 37, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 38, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0196] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 37; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 38; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0197] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 38; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof 90sf-6711731Attorney Docket No.792252001440 comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0198] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 38, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0199] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 38; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 39; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0200] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 41, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 41, and a VL comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 41, and a VL comprising the amino acid sequence of SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 109, or a variant thereof comprising at least about 80% (e.g., at 91sf-6711731Attorney Docket No.792252001440 least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 88. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0201] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 41, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 41. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H1-8 / L1-9 variant IWW, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IWW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 41 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IWW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 41 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IWW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 41 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation 92sf-6711731Attorney Docket No.792252001440 relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IWW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 41 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 109, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0202] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 42; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 43; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0203] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 42, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 43, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution). 93sf-6711731Attorney Docket No.792252001440

[0204] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 42; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 43; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0205] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 43; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0206] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 43, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0207] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 108; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 43; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 44; a VL-CDR1 comprising the amino acid sequence of 94sf-6711731Attorney Docket No.792252001440 SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0208] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 46, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 46, and a VL comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 46, and a VL comprising the amino acid sequence of SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 110, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 88. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0209] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 46, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 46. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H1-8 / L1-9 variant IFW, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IFW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 46 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In 95sf-6711731Attorney Docket No.792252001440 some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IFW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 46 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant IFW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 46 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1- 8 / L1-9 variant IFW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 46 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 110, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino is acid sequence of SEQ ID NO: 61.

[0210] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 48; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0211] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions 96sf-6711731Attorney Docket No.792252001440 (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 48, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0212] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 48; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0213] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 48; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0214] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 48, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising 97sf-6711731Attorney Docket No.792252001440 the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0215] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 48; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0216] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 51, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 51, and a VL comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 51, and a VL comprising the amino acid sequence of SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 111, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 88. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0217] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 51, or a variant thereof comprising at least about 80% 98sf-6711731Attorney Docket No.792252001440 (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 51. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H1-8 / L1-9 variant FME, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant FME comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 51 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant FME comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 51 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant FME comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 51 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1- 8 / L1-9 variant FME comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 51 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 111, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61. 99sf-6711731Attorney Docket No.792252001440

[0218] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 52; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 53; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0219] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 52, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 53, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0220] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 52; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 53; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0221] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 53; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a 100sf-6711731Attorney Docket No.792252001440 VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0222] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 53, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0223] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 53; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 54; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 96.

[0224] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 56, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 56, and a VL comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 56, and a VL comprising the amino acid sequence of SEQ ID NO: 25. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid 101sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 112, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 112, and a VL comprising the amino acid sequence of SEQ ID NO: 88, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 88. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises a VH comprising the amino acid sequence of SEQ ID NO: 112, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the VH and VL do not comprise a signal peptide at the N- terminus.

[0225] In some embodiments, the anti-C5 antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 56, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 56. In some embodiments, the anti-C5 antibody comprises a light chain comprising the amino acid sequence of SEQ ID NO: 25, or a variant thereof comprising at least about 80% (e.g., at least about any of 85%, 90%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to SEQ ID NO: 25. In some embodiments, the anti-C5 antibody is humanized. In some embodiments, the anti-C5 antibody is a chimeric antibody. In some embodiments, the anti-C5 antibody is mAb H1-8 / L1-9 variant FMW, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant FMW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 56 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 112, and a VL comprising the amino acid sequence of SEQ ID NO: 88. In some embodiments, the anti-C5 antibody comprises an Fc fragment. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment or variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant FMW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 56 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 112, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment comprising the amino acid sequence of SEQ ID NO: 32. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, or a variant thereof. In some embodiments, the anti-C5 antibody mAb H1-8 / L1-9 variant FMW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 56 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 112, and a 102sf-6711731Attorney Docket No.792252001440 VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 33. In some embodiments, the anti-C5 antibody comprises a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, or variant thereof. In some embodiments, the anti-C5 antibody mAb H1- 8 / L1-9 variant FMW comprises i) a heavy chain comprising the amino acid sequence of SEQ ID NO: 56 and a light chain comprising the amino acid sequence of SEQ ID NO: 25; or ii) a VH comprising the amino acid sequence of SEQ ID NO: 112, and a VL comprising the amino acid sequence of SEQ ID NO: 88; and a human IgG4 Fc fragment having an S108P mutation, a M308L mutation, and a N314A mutation relative to SEQ ID NO: 32, comprising the amino acid sequence of SEQ ID NO: 61.

[0226] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises at least one of the CDRs selected from the group consisting of: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs. In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to 5 (e.g., 5, 4, 3, 2, or 1) amino acid variations (e.g., insertion, deletion, substitution, such as conserved substitution) in one or more of the CDRs.

[0227] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid 103sf-6711731Attorney Docket No.792252001440 sequence of SEQ ID NO: 49, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL- CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution).

[0228] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9, or a variant thereof comprising up to about 3 (such as about any of 1, 2, or 3) amino acid substitutions (e.g., conserved substitution); and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0229] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 47; a VH- CDR2 comprising the amino acid sequence of SEQ ID NO: 57; a VH-CDR3 comprising the amino acid sequence of SEQ ID NO: 49; a VL-CDR1 comprising the amino acid sequence of SEQ ID NO: 23; a VL-CDR2 comprising the amino acid sequence of SEQ ID NO: 9; and a VL-CDR3 comprising the amino acid sequence of SEQ ID NO: 10.

[0230] In some embodiments, the anti-C5 antibody or antigen-binding fragment thereof comprises at least one of the CDRs selected from the group consisting of: a VH-CDR1 comprising the amino acid sequence of SEQ ID NO: 86; a VH-CDR2 comprising the amino acid sequence of SEQ ID NO: 57; a VH-CDR3 comprising the amino acid sequ...

Claims

Attorney Docket No.792252001440 CLAIMS 1. A method of treating a complement-mediated disease in a human individual, comprising administering to the human individual an effective amount of a fusion protein comprising i) an antibody moiety that specifically binds to human C5 (“anti-C5 antibody moiety”) and ii) a Factor H (FH) or functional fragment thereof, wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, and wherein: a) the human individual has a body weight of less than 80 kg, and the fusion protein is administered at a maintenance dose of about 1,920 mg at the maintenance phase; or b) the human individual has a body weight of 80 kg or greater, and the fusion protein is administered at a maintenance dose of about 2400 mg at the maintenance phase.

2. The method of claim 1, wherein the fusion protein is administered intravenously (IV) at the initial phase and is administered subcutaneously (SC) at the maintenance phase.

3. The method of claim 1 or 2, wherein the initial phase comprises administering a single initial dose of the fusion protein on Day 1, and the maintenance phase comprises administering a maintenance dose of the fusion protein biweekly (Q2W) starting Day 8 for at least about 13 weeks.

4. The method of any one of claims 1-3, wherein the human individual’s body weight is less than 80 kg, and the maintenance dose of the fusion protein is about 1,920 mg.

5. The method of any one of claims 1-3, wherein the human individual’s body weight is 80 kg or greater, and the maintenance dose of the fusion protein is about 2,400 mg.

6. The method of any one of claims 1-5, wherein the initial phase comprises administering the fusion protein at a single initial dose of about 2,400 mg IV.

7. The method of any one of claims 1-4 and 6, wherein the human individual’s body weight is less than 80 kg, and wherein the method comprises administering the fusion protein at a single initial dose of about 2,400 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 1,920 mg SC biweekly starting Day 8 for at least about 13 weeks for the maintenance phase. 153sf-6711731Attorney Docket No.792252001440 8. The method of any one of claims 1-3, 5, and 6, wherein the human individual’s body weight is 80 kg or greater, and wherein the method comprises administering the fusion protein at a single initial dose of about 2,400 mg IV on Day 1 for the initial phase, and administering the fusion protein at a maintenance dose of about 2,400 mg SC biweekly starting Day 8 for at least about 13 weeks for the maintenance phase.

9. A method of treating a complement-mediated disease in a human individual, comprising administering to the human individual an effective amount of a fusion protein comprising i) an antibody moiety that specifically binds to human C5 (“anti-C5 antibody moiety”) and ii) a Factor H (FH) or functional fragment thereof, wherein the method comprises administering the fusion protein at an initial phase and a maintenance phase, and wherein the fusion protein is administered at a maintenance dose of about 200 mg to about 720 mg at the maintenance phase.

10. The method of claim 9, wherein the fusion protein is administered intravenously (IV) at the initial phase and is administered intravenously (IV) or subcutaneously (SC) at the maintenance phase.

11. The method of claim 9 or 10, wherein the fusion protein is administered weekly (QW) or biweekly (Q2W) at the maintenance phase.

12. The method of claims 9-11, wherein the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg IV on Day 1.

13. The method of claims 9-12, wherein the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of: i) about 270 mg IV to about 720 mg IV biweekly; or ii) about 270 mg SC to about 720 mg SC weekly.

14. The method of any one of claims 9-13, wherein the maintenance phase is at least about 12 weeks.

15. The method of any one of claims 9-14, wherein the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg IV on Day 1, and wherein the maintenance phase comprises: 154sf-6711731Attorney Docket No.792252001440 (i) administering the fusion protein starting Day 8 at a maintenance dose of about 300 mg SC to about 540 mg SC weekly for at least 12 weeks; or (ii) administering the fusion protein starting Day 8 at a maintenance dose of about 270 mg IV to about 450 mg IV biweekly for at least about 13 weeks.

16. The method of any one of claims 9-15, wherein the initial phase comprises administering the fusion protein at an initial dose of about 1200 mg IV on Day 1, and wherein: (i) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 300 mg SC weekly for at least about 12 weeks; (ii) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 420 mg SC weekly for at least about 12 weeks; (iii) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 540 mg SC weekly for at least about 12 weeks; (iv) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 270 mg IV biweekly for at least about 13 weeks; (v) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 360 mg IV biweekly for at least about 13 weeks; or (vi) the maintenance phase comprises administering the fusion protein starting Day 8 at a maintenance dose of about 450 mg IV biweekly for at least about 13 weeks.

17. The method of any one of claims 1-16, wherein the complement-mediated disease is selected from the group consisting of paroxysmal nocturnal hemoglobinuria (PNH) syndrome, C3 glomerulopathy (C3G), IgA nephropathy (IgAN), thrombotic microangiopathy secondary to systemic lupus erythematosus (SLE-TMA), and thrombotic microangiopathy secondary to lupus nephritis (LN-TMA).

18. The method of any one of claims 1-8 and 17, wherein the complement-mediated disease is PNH.

19. The method of any one of claims 9-17, wherein the complement-mediated disease is IgAN.

20. The method of any one of claims 1-19, wherein the binding of the anti-C5 antibody moiety to human C5 is pH-dependent, and wherein the anti-C5 antibody moiety binds more strongly to human C5 at a neutral pH than it does at an acidic pH. 155sf-6711731Attorney Docket No.792252001440 21. The method of any one of claims 1-20, wherein the anti-C5 antibody moiety is a full- length antibody, a Fab, a Fab’, a F(ab)2, a F(ab’)2, an scFv, or a combination thereof.

22. The method of claim 21, wherein the anti-C5 antibody moiety is a full-length antibody (“anti-C5 full-length antibody”).

23. The method of claim 22, wherein the anti-C5 full-length antibody comprises an Fc fragment derived from human IgG4.

24. The method of claim 23, wherein the Fc fragment comprises the amino acid sequence of any of SEQ ID NOs: 32, 33, and 61.

25. The method of claim 24, wherein the Fc fragment comprises the amino acid sequence of SEQ ID NO:

61.

26. The method of any one of claims 22-25, wherein the anti-C5 full-length antibody comprises a heavy chain and a light chain, and wherein: (i) the heavy chain comprises the amino acid sequence of SEQ ID NO: 119, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (ii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 121, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (iii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 123, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (iv) the heavy chain comprises the amino acid sequence of SEQ ID NO: 125, and the light chain comprises the amino acid sequence of SEQ ID NO: 74; (v) the heavy chain comprises the amino acid sequence of SEQ ID NO: 120, and the light chain comprises the amino acid sequence of SEQ ID NO: 90; (vi) the heavy chain comprises the amino acid sequence of SEQ ID NO: 122, and the light chain comprises the amino acid sequence of SEQ ID NO: 90; (vii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 124, and the light chain comprises the amino acid sequence of SEQ ID NO: 90; or (viii) the heavy chain comprises the amino acid sequence of SEQ ID NO: 126, and the light chain comprises the amino acid sequence of SEQ ID NO:

90.

27. The method of any one of claims 1-26, wherein the functional fragment of FH comprises short consensus repeat (SCR) domains 1-5 of FH. 156sf-6711731Attorney Docket No.792252001440 28. The method of claim 27, wherein the functional fragment of FH comprises the amino acid sequence of SEQ ID NO:

85.

29. The method of any one of claims 22-28, wherein the fusion protein comprises a first FH or functional fragment thereof and a second FH or functional fragment thereof, wherein the first FH or functional fragment thereof is fused to the C-terminus of a first heavy chain of the anti-C5 full-length antibody, and the second FH or functional fragment thereof is fused to the C-terminus of a second heavy chain of the anti-C5 full-length antibody.

30. The method of claim 29, wherein: (i) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 72, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (ii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 76, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (iii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 78, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (iv) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 80, each light chain comprises the amino acid sequence of SEQ ID NO: 74; (v) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 89, each light chain comprises the amino acid sequence of SEQ ID NO: 90; (vi) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 116, each light chain comprises the amino acid sequence of SEQ ID NO: 90; (vii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 117, each light chain comprises the amino acid sequence of SEQ ID NO: 90; or (viii) each heavy chain fused to FH or functional fragment thereof comprises the amino acid sequence of SEQ ID NO: 118, each light chain comprises the amino acid sequence of SEQ ID NO:

90. 157sf-6711731Attorney Docket No.792252001440 31. The method of any one of claims 1-30, wherein the fusion protein is formulated in a pharmaceutical composition, wherein the pharmaceutical composition comprises about 120 mg / mL of the fusion protein, sodium phosphate, sodium chloride, L-Lys-HCL, and polysorbate 80, pH of about 6.

0. 158sf-6711731

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