UDP-glucosyltransferase variant, UDP-glucosyltransferase variant fusion enzyme, and use thereof for producing steviol glycoside

UDP-glucosyltransferase variants with specific amino acid substitutions enhance enzyme activity, addressing inefficiencies in stevia extract production by increasing the yield of high-value steviol glycosides like Reb M, thus improving the economic viability and quality of stevia-derived sweeteners.

WO2026089391A1PCT designated stage Publication Date: 2026-04-30SAMYANG CORP
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Patent Information

Application Number
PCT/KR2025/016390
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-10-22
Filing Date
2025-10-16
Publication Date
2026-04-30

AI Technical Summary

Technical Problem

Existing stevia extract production methods suffer from inefficiencies in recovering desired steviol glycosides like Reb M, and there is a need for improved production of Reb M precursors due to variations in soil and climate conditions, leading to off-flavors and labor-intensive purification processes.

Method used

Development of UDP-glucosyltransferase variants and fusion proteins with specific amino acid substitutions, such as at positions 99, 376, and 379, to enhance enzyme activity for converting stevioside, Reb A, and Reb E to Reb D and Reb M, enabling high-yield production of steviol glycosides.

Benefits of technology

The variants and fusion proteins increase the productivity of steviol glycosides, particularly Reb M, ensuring economic viability and efficient conversion reactions through simultaneous enzyme activity, thereby improving the quality and yield of stevia-derived sweeteners.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to a UDP-glucosyltransferase variant, a fusion enzyme of UDP-glucosyltransferase variants, and a use thereof for producing steviol glycoside. The UDP-glucosyltransferase variant and the fusion enzyme of UDP-glucosyltransferase variants according to the present invention have improved enzymatic activity. In addition, the fusion enzyme of UDP-glucosyltransferase variants has further improved enzymatic activity compared to when each variant is used individually. Accordingly, the UDP-glucosyltransferase variant and the UDP-glucosyltransferase variant fusion enzyme according to the present invention can be widely applied in the field of steviol glycoside synthesis, and have excellent commercial value and significance in that production efficiency can be increased and production costs can be reduced.
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Citation Information

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  • Production of steviol glycosides in recombinant hosts

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