Universal microbial lysis method compatible with hybridization based nucleic acid enrichment and extraction techniques
A combination of ultrasound, enzymatic, and thermal steps in microbial lysis addresses the incompatibility of chaotropes with hybridization-based extraction, achieving efficient nucleic acid release for diverse microbes in metagenomic analysis.
WO2026136415A1 Publication Date: 2026-06-25SIEMENS HEALTHCARE DIAGNOSTICS INC
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Patent Information
- Application Number
- PCT/US2025/059919
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-12-20
- Filing Date
- 2025-12-16
- Publication Date
- 2026-06-25
AI Technical Summary
Technical Problem
Existing microbial lysis methods are incompatible with hybridization-based nucleic acid extraction, as chaotropes and detergents used in conventional methods hydrolyze hydrogen bonds, and existing methods lack universality across different microbial types.
Method used
A combination of ultrasound sonication, enzymatic treatment, and thermal steps, without chaotropes or detergents, is used to lyse a wide variety of microbes, ensuring compatibility with hybridization-based nucleic acid extraction.
Benefits of technology
The method efficiently releases nucleic acids from 1 CFU/mL to 10000 CFU/mL of various microbes, enabling unbiased metagenomic analysis and detection, compatible with hybridization-based methods.
✦ Generated by Eureka AI based on patent content.
Abstract
The present disclosure provides an in-vitro method of universal microbial lysis compatible with hybridization-based nucleic acid enrichment and extraction methods, comprising the steps of: (a) subjecting a sample to a combination of lysis steps to obtain a lysed sample, wherein the combination of lysis steps is selected from the group consisting of: (i) an ultrasound sonication, (ii) a combination of multiple sonication steps,- (iii) a combination of enzymatic treatment and one or more sonication steps, and (iv) a combination of enzymatic treatment, one or more sonication steps, and thermal step; and (b) treating the lysed sample of step (a) with one or more enzymes, followed by sonication for microbial lysis.
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