Method for preparing efficient oligomerized astragalus polysaccharide by carboxymethylated molecular modification

A technology of carboxymethylation and astragalus polysaccharides, which is applied in the field of preparation and structural modification of bioactive polysaccharides, can solve the problems of poor immune regulation activity and antioxidant capacity, waste of time, waste of raw materials, etc., and achieve good regulation of activity and antioxidation The effect of energy saving, time saving and material saving

CN103030704AInactive Publication Date: 2013-04-10NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2013-04-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing efficient oligomerized astragalus polysaccharide by carboxymethylated molecular modification, which relates to the technical field of preparation and structural improvement of biological active polysaccharides. The preparation method comprises the following steps: carrying out carboxymethylated molecular modification by using a sodium hydroxide-monochloro acetic acid system; using product volume, carboxymethyl substitutivity and immune regulatory function as indexes, using reaction temperature, reagent proportioning and reaction time as factors, and optimizing the optimal condition of the sodium hydroxide-monochloro acetic acid modification for astragalus polysaccharide by an orthogonal test as reaction temperature at 65 DEG C, proportion of sodium hydroxide and monochloro acetic acid: 1.6:1, and reaction time 3.5h; slowly adding astragalus decocted liquor to ethanol to meet the final concentration of 50%, and removing precipitate; adding ethanol into liquid supernatant to meet the final concentration of 60%; taking precipitate to obtain coarse polysaccharide; and modifying purified astragalus polysaccharide by optimized condition. Compared with feeding experiment and research, the growth promoting and immunocompetence enhancing activity of purified astragalus polysaccharide after carboxymethylated molecular modification is the strongest. The oligomerized astragalus polysaccharide has good regulatory activity and resistance to oxidation, and materials and time are saved.
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Description

Technical field:

[0001] The invention relates to the technical field of preparation and structural transformation of biologically active polysaccharides, in particular to a method for preparing high-efficiency oligomeric astragalus polysaccharides through carboxymethylation molecular modification. Background technique:

[0002] Studies in recent years have found that astragalus contains polysaccharides, saponins, flavonoids, amino acids and other active ingredients. These active ingredients can promote antibody production and immune response. Among them, astragalus polysaccharides are the most reported. Astragalus polysaccharide is one of the main active components of Astragalus, obtained through high-tech extraction and separation.

[0003] The currently extracted astragalus polysaccharides have poor immunoregulatory activity and antioxidant capacity, which wastes raw materials and time. Invention content:

[0004] The purpose of the present invention is to provide a met...

Examples

Embodiment

[0031] 1. Materials and methods:

[0032] 1.1 Test animals and materials:

[0033] 120 AA (Arbor Acres) male broiler chickens (1 day old) were randomly divided into 2 treatments, each treatment had 6 replicates, and each replicate had 10 chickens. 23 hours of light per day, free access to food and water. Feeding was carried out according to normal feeding standards.