A gene circuit structure for the detection of glioma markers

A brain glioma and gene circuit technology, applied in the field of synthetic biology, can solve the problems of high cost and experimental requirements, and achieve the effect of simple structure, good logic judgment function, and stable output

CN104101628BActive Publication Date: 2016-08-24BEIHANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2016-08-24

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Abstract

A gene circuit structure for detecting glioma markers, which consists of a single-index detection module and a fusion circuit module, and the two modules are sequentially connected to each other; the single-index detection module is composed of an inverter, The inverter is composed of an input terminal, an output terminal and a reverse module, and its function is to reverse the input signal and output the reverse signal; the fusion circuit is composed of a three-port input NAND gate, the The NAND gate is a NAND operation on the input. When the three inputs are all high level, the power circuit outputs a low level signal. The invention has simple structure, fast response speed, stable output and good performance, overcomes unfavorable factors such as interference when a single module works independently, and realizes the detection and alarm functions of brain glioma markers. It has good practical value and broad application prospect in the technical field of synthetic biology.
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Description

technical field

[0001] The invention relates to a gene circuit structure for realizing the detection of brain glioma markers, and belongs to the technical field of synthetic biology. Background technique

[0002] Glioma (glioma) includes a variety of different types of malignant brain tumors, accounting for about 50% to 60% of human primary brain tumors, with high morbidity and mortality. Gliomas of the human brain become more common with age. The increase in permeability of brain gliomas correlates with their malignancy. Malignant gliomas are characterized by their tendency to invade and recur easily.

[0003] A class of macromolecular substances closely related to the occurrence of cancer tumors is called tumor markers (Tumor Markers). Tumor markers can be detected from blood or body fluids, and their components are mainly macromolecular proteins, including tumor-associated antigens, ectopic hormones, enzymes, and changes in the host anti-tumor immune cascade. There ar...

Examples

Embodiment Construction

[0020] See figure 1 , figure 2 , image 3 As shown, a gene circuit structure for realizing the detection of glioma markers is implemented as follows: it consists of a single index detection module and a fusion circuit module, and the two modules are sequentially connected to each other. The single index detection module is composed of an inverter. The inverter is composed of an input terminal, an output terminal and an inverting module, and its main function is to invert the input signal and output the inverse signal. This module detects the three glioma markers GFAP, YKL-40 and CD133 respectively, the input terminals are In1, In2 and In3 respectively, and the corresponding output terminals are OUT1, OUT2 and OUT3 respectively. The fusion circuit module is composed of a three-port input NAND gate. The expression of GFP (green fluorescent protein) is regulated by the input of three proteins A, B, and C. When the concentrations of A, B, and C are high, the expression of GFP...