A bacterium for inhibiting the growth of Gambia algae and its application

Abstract

The present invention discloses a bacterium for inhibiting the growth of Gambian algae and its application. The bacterial strain for inhibiting the growth of Gambian algae provided by the present invention is Gamma proteobacterium XZX-S5, and the deposit number of Gamma proteobacterium XZX-S5 is GDMCC No.60157. The present invention has the following advantages: (1) Gamma proteobacterium XZX-S5 is screened from the environment and belongs to the symbiotic bacteria of Gambian algae. Its use does not generate environmental pressure and does not cause secondary pollution to the ecology; (2) Gamma proteobacterium XZX-S5 bacterial agent can not only inhibit the biomass of Gambian algae, but also has the potential to reduce the secretion of ciguatera toxin.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and relates to a bacterial strain related to algae growth inhibition, in particular to a Gamma proteobacterium XZX-S5 for inhibiting the growth of Gambia algae and an application thereof. Background Art

[0002] Ciguatera fish poisoning (CFP) is a fat-soluble high ether substance with extremely strong toxicity. It is one of the known red tide biotoxins with more serious hazards. It is colorless, odorless, fat-soluble, insoluble in water, heat-resistant, and not easily destroyed by gastric acid. It is mainly produced by benthic Gambierdiscus sp. The toxin has a food chain accumulation effect. After the toxin is produced by Gambierdiscus sp., it is ingested by fish and enriched in the fish body, and eventually threatens human health through the food chain. With the expansion of the scale of global fishery trade, poisoning incidents have increased year by year. Since ciguatera toxin is a fat-soluble toxin, it is difficult to metabolize and eliminate in the body, so it is very difficult to treat. Ciguatera toxin poisoning can cause dysfunction of the nervous system, cardiovascular system, and gastrointestinal system, which is extremely harmful to human health.

[0003] At present, the production mechanism and prevention and control measures of ciguatera toxins have become a hot topic of global concern. In recent years, based on the development of microbial ecology, people have realized that microorganisms play an important role in regulating the growth and toxin production characteristics of algae. On the one hand, some microorganisms can help algae grow and promote the secretion of toxins; on the other hand, some microorganisms can also inhibit the growth of algae and reduce the secretion of toxins. Summary of the invention

[0004] The technical problem to be solved by the present invention is how to inhibit the growth of Gambia algae that secretes ciguatoxin.

[0005] In order to solve the above technical problems, the present invention first provides a bacterium related to algae growth inhibition and attenuation: Gamma proteobacterium XZX-S5.

[0006] The deposit number of the Gamma proteobacterium XZX-S5 provided by the invention is GDMCC No.60157.

[0007] The classification name of the gamma proteobacterium Gamma proteobacterium XZX-S5 provided by the present invention is Gamma proteobacterium. Gamma proteobacterium Gamma proteobacterium XZX-S5 has been deposited in Guangdong Microbiological Culture Collection Center (GDMCC for short, address: No. 100, Xianlie Middle Road, Guangzhou, Guangdong Institute of Microbiology, 5th Floor, Building 59, Postal Code: 510070) on April 1, 2017, and the deposit number is GDMCC No.60157. The gamma proteobacterium Gamma proteobacterium XZX-S5 is a Gram-negative bacterium with a light yellow colony, a smooth surface and wet edges.

[0008] In order to solve the above technical problems, the present invention provides a new use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product.

[0009] The invention provides application of Gamma proteobacterium or its bacterial agent or its bacterial suspension or its culture solution or its fermentation product in inhibiting the growth of Gambia algae.

[0010] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in preparing a product for inhibiting the growth of Gambia algae.

[0011] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or its bacterial suspension or its culture solution or its fermentation product in inhibiting the biomass of Gambia algae.

[0012] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or its bacterial suspension or its culture solution or its fermentation product in preparing a product for inhibiting the biomass of Gambian algae.

[0013] In the above application, the inhibition of the growth of Gambia algae or the inhibition of the biomass of Gambia algae is specifically embodied in reducing the cell density or number of Gambia algae.

[0014] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in reducing the photosynthetic capacity of Gambia algae.

[0015] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in preparing a product for reducing the photosynthetic capacity of Gambia algae.

[0016] In the above application, the reducing of the photosynthetic capacity of Gambia algae is specifically embodied in reducing the total amount of chlorophyll in Gambia algae cells.

[0017] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or its bacterial suspension or its culture solution or its fermentation product in reducing the photosynthetic efficiency of Gambia algae.

[0018] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or its bacterial suspension or its culture solution or its fermentation product in preparing a product that reduces the photosynthetic efficiency of Gambia algae.

[0019] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in regulating the element composition ratio in Gambia algae cells.

[0020] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in preparing a product for regulating the element composition ratio in Gambia algae cells.

[0021] In the above application, the regulation of the element composition ratio in the Gambian algae cells is specifically embodied in reducing the content of phosphorus and / or sulfur and / or potassium and / or calcium and / or iron and / or manganese in the Gambian algae cells.

[0022] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in reducing the ability of Gambia algae to secrete ciguatera toxin.

[0023] The present invention also provides the use of Gamma proteobacterium or its bacterial agent or bacterial suspension or culture solution or fermentation product in preparing a product that reduces the ability of Gambia algae to secrete ciguatoxin.

[0024] In the above application, the Gamma proteobacterium is the Gamma proteobacterium XZX-S5.

[0025] In order to solve the above technical problems, the present invention also provides a product.

[0026] The active ingredient of the product provided by the present invention is the above-mentioned Gamma proteobacterium XZX-S5 or its bacterial agent or bacterial suspension or culture solution or fermentation product;

[0027] The product has any one of the following functions 1)-6):

[0028] 1) Inhibit the growth of Gambia algae;

[0029] 2) inhibiting the biomass of Gambiae algae;

[0030] 3) Reduce the photosynthetic capacity of Gambia algae;

[0031] 4) Reduce the photosynthetic efficiency of Gambia algae;

[0032] 5) Regulate the element composition ratio in Gambia algae cells;

[0033] 6) Reduce the ability of Gambia algae to secrete ciguatera toxins.

[0034] In the above-mentioned product, the inhibition of the growth of Gambia algae or the inhibition of the biomass of Gambia algae is specifically embodied in reducing the density or number of Gambia algae cells;

[0035] The reducing of the photosynthetic capacity of Gambia algae is specifically embodied in reducing the total amount of chlorophyll in Gambia algae cells;

[0036] The regulating the element composition in the Gambian algae cells is specifically embodied in reducing the content of phosphorus and / or sulfur and / or potassium and / or calcium and / or iron and / or manganese in the Gambian algae cells.

[0037] In order to solve the above technical problems, the present invention finally provides a method for inhibiting the growth of Gambia algae.

[0038] The invention provides a method for inhibiting the growth of Gambia algae, comprising the step of co-culturing Gammaproteobacterium XZX-S5 or its bacterial agent or its bacterial suspension or its culture solution or its fermentation product with Gambia algae.

[0039] In the above method, the co-cultivation method is to add Gamma proteobacterium XZX-S5 to the Gambia algae culture system for co-cultivation to obtain a co-cultivation system. The concentration of Gamma proteobacterium XZX-S5 in the co-cultivation system is 5×10 7 -5×10 9 bacteria / mL -1The concentration of the algae cells in the co-culture system is 1000 cell / mL -1 .

[0040] In the above application, product or method, the preparation method of the Gamma proteobacterium XZX-S5 bacterial agent is as follows: Gamma proteobacterium XZX-S5 is fermented and cultured in LB liquid culture medium to obtain the XZX-S5 bacterial agent. The OD of the XZX-S5 bacterial agent is 600 The fermentation temperature can be 27-30°C, specifically 30°C; the fermentation time can be 24-36h, specifically 30h; the fermentation method is rotary shaking culture, the rotation speed of the rotary shaking culture can be 180-200 rpm, specifically 180 rpm, and the rotation radius is 13.5cm.

[0041] The present invention has the following advantages: (1) The algae-inhibiting strain is derived from the natural environment where Gambia algae grow, and will not cause secondary pollution to the environment, and there is no ecological risk; (2) Gammaproteus (or its metabolites) not only inhibits the growth of algae, but also has the potential to reduce the secretion of ciguatera toxins. (3) Gammaproteus (or its metabolites) changes the elemental composition ratio of Gambia algae, affecting its physiological characteristics.

[0042] The present invention screens strains that inhibit the growth of Gambia algae and reduce the toxin release from the symbiotic bacteria of Gambia algae. From the perspective of prevention and control, it is helpful to obtain a ciguatera toxin prevention and control method based on "biological control". The number of algae symbiotic bacteria is huge, providing a resource library for screening algae-inhibiting strains; mining strains with algae-inhibiting ability and reduced toxin synthesis ability will find a new way out for the prevention and control of ciguatera toxins. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is the morphological identification of strain XZX-S5.

[0044] Figure 2 This is the effect of strain XZX-S5 on the cell morphology of Gambiae algae.

[0045] Figure 3 The inhibitory effect of adding Gamma proteobacterium XZX-S5 on the growth of Gambia algae. The horizontal axis is the culture time, and the vertical axis is the algae cell density.

[0046] Figure 4The figure shows the inhibitory effect of Gamma proteobacterium XZX-S5 on photosynthesis of Gambia algae. The horizontal axis is the culture time of algae cells, and the vertical axis is the total amount of chlorophyll in algae cells.

[0047] Figure 5 The inhibitory effect of Gamma proteobacterium XZX-S5 on the photosynthetic efficiency of Gambia algae. The horizontal axis represents the algae cell culture time, and the vertical axis represents the algae cell photosynthetic efficiency (FV / FM).

[0048] Figure 6 The effect of Gamma proteobacterium XZX-S5 on the element composition of Gambia algae. The horizontal axis is the experimental group, and the vertical axis is the main element composition of algae cells.

[0049] Collection Instructions

[0050] Bacterial species name: Gammaproteus

[0051] Latin name: Gamma proteobacterium

[0052] Strain ID: XZX-S5

[0053] Depository: Guangdong Microbial Culture Collection Center

[0054] Depository Abbreviation: GDMCC

[0055] Address: 5th Floor, Building 59, Guangdong Institute of Microbiology, No. 100, Xianlie Middle Road, Guangzhou

[0056] Deposit date: April 1, 2017

[0057] Registration number of the depository center: GDMCC No.60157 DETAILED DESCRIPTION

[0058] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0059] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0060] The quantitative tests in the following examples were all repeated three times, and the results were averaged.

[0061] The Gambierdiscus sp.1112M1M03 (type 6) in the following examples was kindly donated by the State Key Laboratory of Ocean Pollution, City University of Hong Kong, and is recorded in the document "The Effect of Microorganisms on the Growth of Dinoflagellates and the Regional Distribution of Ciguatera Toxicity, 2015, 15(3):401-412". The public can obtain it from the applicant. The biological material is only used to repeat the relevant experiments of the present invention and cannot be used for other purposes. Gambierdiscus sp.1112M1M03 was cultured using Kmedium algae culture medium (salinity adjusted to 31‰), and seawater was filtered through a Whatman 0.22μm polycarbonate filter and sterilized at 121°C for 20min. The culture conditions of Gambierdiscus sp.1112M1M03 were as follows: temperature was 25°C, cold white fluorescent lamps were used, light intensity was 3-4%, and light-dark cycle was 12h:12h. Use 250mL conical flask for static culture. When the algal cell concentration exceeds 2500cell·mL -1 Transfer when.

[0062] The LB solid medium in the following examples: 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, 1000 ml of deionized water, 15 g of bacteriological agar powder, pH 7.0.

[0063] The LB liquid culture medium in the following examples includes 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, 1000 mL of deionized water, and pH 7.0.

[0064] The K medium algae culture medium in the following examples is a product of Shanghai Guangyu Biotechnology Co., Ltd.

[0065] Example 1. Isolation and identification of strain XZX-S5

[0066] 1. Isolation, culture and screening of algal cell symbiotic bacteria

[0067] The Gambierdiscus sp.1112M1M03 was aseptically taken out, shaken and left to stand for a while (3-5 minutes), the supernatant was taken and diluted in multiples, and then plated on a clean bench to obtain a culturable bacterial monoclonal. The selected monoclonal strain was cultured and then co-cultured with the Gambierdiscus sp.1112M1M03. After culture, the number of algal cells, chlorophyll content and photosynthetic efficiency were detected. The strains that inhibited the growth of Gambierdiscus were marked as potential algae-inhibiting strains, and the most obvious algae-inhibiting strain was selected and named XZX-S5.

[0068] 2. Identification of XZX-S5 strain

[0069] 1. Morphological identification

[0070] The XZX-S5 strain isolated in the above step 1, which is in the logarithmic growth phase and has uniform colony size, is described for single colony status, mainly including colony size, color, transparency, wetness, colony surface status (whether flat, protruding, wrinkled, concave, etc.) and colony edge status (whether neat, irregular, radial, etc.).

[0071] The results showed that the colonies of XZX-S5 strain were light yellow, with moist surfaces and flat edges ( Figure 1 ).

[0072] 2. Molecular identification

[0073] The XZX-S5 strain isolated in step 1 was cultured according to conventional methods, and the total DNA of the XZX-S5 strain was extracted as a template. The universal primers 27f and 1492R were used for PCR amplification to obtain PCR products. The primer sequences are as follows:

[0074] 27f: 5'-AGAGTTTGATCMTGGCTCAG-3';

[0075] 1492R: 5'-TACGGYTACCTTTGTTACGACTT-3'.

[0076] PCR reaction system (30 μL): Buffer 3 μL, dNTP 2 μL, 27f and 1492R 3 μL each, DNA template 1 μL, enzyme 0.2 μL.

[0077] PCR reaction conditions: pre-denaturation at 95°C for 5 min; denaturation at 95°C for 30 sec, annealing at 55°C for 30 sec, extension at 72°C for 1 min, for a total of 35 cycles; extension at 72°C for 10 min.

[0078] The PCR products were detected by 1% gel electrophoresis, the electrophoresis conditions were: 3 μL sample + 1% agarose gel, the marker band composition was: 100 bp; 250 bp; 500 bp; 750 bp; 1000 bp; 2000 bp; 3000 bp; 5000 bp. The PCR products were sequenced. The 16S rDNA sequence of the XZX-S5 strain is shown in Sequence 1. The homology analysis of the 16S rRNA sequence was completed by Shenzhen Hengchuang Gene Technology Co., Ltd., and the sequence alignment was completed online through the NCBI database of the National Center for Biotechnology Information of the United States (http: / / www.ncbi.nlm.nih.gov).

[0079] In view of the above morphological identification and molecular identification, the XZX-S5 strain isolated and screened in step 1 was identified for species, and the identification result was: the XZX-S5 strain was a gammaproteobacterium, and the classification name was Gamma proteobacterium. The gammaproteobacterium Gamma proteobacterium XZX-S5 was deposited in Guangdong Microbiological Culture Collection Center (GDMCC for short, address: 100 Xianlie Middle Road, Guangzhou, Guangdong Institute of Microbiology, 5th Floor, Building 59, Postal Code: 510070) on April 1, 2017, and the deposit number was GDMCC No.60157.

[0080] Example 2: Gamma proteobacterium XZX-S5 inhibits the growth of Gambia spp.

[0081] 1. Preparation of experimental materials and experimental methods

[0082] 1. Preparation of experimental materials

[0083] The present invention selects Gambierdiscus sp.1112M1M03, which has strong ciguatera toxin production, as an experimental object to study the inhibitory effect of Gamma proteobacterium XZX-S5 on the growth of Gambierdiscus.

[0084] (1) Cultivation of Gambierdiscus sp.1112M1M03

[0085] Use a micropipette to draw 10μL of Gambierdiscus sp.1112M1M03 algae solution onto a glass slide, and observe the individual conditions of Gambierdiscus sp. under a 40x optical microscope. After confirming that the growth is in good condition, transfer 20ml of Gambierdiscus algae solution to 100mL of fresh K medium for expansion. Count the algae cell concentration every two days. The counting method is as follows: Gently shake the cell conical flask to evenly distribute the Gambierdiscus in the algae solution, pipette 10μL of algae solution and spread it on a clean glass slide, and use a counter to count the Gambierdiscus cells under a 40x optical microscope Leica MA 16FA. When the algae cell concentration exceeds 2000cell mL -1 At the same time, 20 mL of the Gambia algae solution was transferred to a conical flask containing 100 mL of fresh K medium for the second round of expansion culture. Finally, all 100 mL of the algae solution was transferred to a conical flask containing 400 mL of fresh K medium for culture, and 500 mL of the Gambia algae culture system was used for the following growth inhibition test.

[0086] (2) Preparation of XZX-S5 bacterial agent

[0087] 1) Inoculate 100 μL of XZX-S5 bacterial solution into 5 mL of LB liquid culture medium and culture overnight in a constant temperature shaker (30° C., 180 rpm, rotation radius of 13.5 cm) to obtain primary seeds.

[0088] 2) Inoculate the primary seeds into 250 mL LB liquid medium (100 mL of liquid volume) and culture in a constant temperature shaker for 30 hours to obtain XZX-S5 inoculum. Aseptic sampling was used to measure the OD of XZX-S5 inoculum using a spectrophotometer. 600 value, making it OD 600 The value reaches 0.2-0.5.

[0089] 2. Bacteria addition experiment

[0090] The XZX-S5 bacterial agent was added to the Gambia algae culture system for co-cultivation to obtain a co-cultivation system. According to the experimental design, the XZX-S5 bacterial agent was added at the beginning of the logarithmic growth of the Gambia algae. At this time, the Gambia algae grew vigorously and was about to enter the logarithmic growth period. The algae cell concentration was 1000 cell / mL -1 In the addition experiment, according to the different concentrations of XZX-S5 bacteria, the following control group, low concentration group, medium concentration group and high concentration group were set:

[0091] Control group: The final concentration of XZX-S5 bacteria in the co-culture system was 0 bacteria / mL -1 ;

[0092] Low concentration group (5×10 7 bacteria / mL -1 ): The final concentration of XZX-S5 bacteria in the co-culture system was 5×10 7 bacteria / mL -1 ;

[0093] The medium concentration group (5×10 8 bacteria / mL -1 ): The final concentration of XZX-S5 bacteria in the co-culture system was 5×10 8 bacteria / mL -1 ;

[0094] High concentration group (5×10 9 bacteria / mL -1 ): The final concentration of XZX-S5 bacteria in the co-culture system was 5×10 9 bacteria / mL -1 .

[0095] 2. Gamma proteobacterium XZX-S5 inhibits the growth of Gambia

[0096] 1. Morphological detection of algal cells

[0097] The individual morphologies of Gambiae algae cells in the experimental group and the control group were observed under a microscope when each co-culture system was cultured for 48-72 hours.

[0098] The observation results of algal cell morphology are as follows Figure 2 As shown in the figure, when XZX-S5 was not added (control group), the Gambia algae cells were intact in morphology, uniform in color, and relatively uniform in size, reflecting a good physiological state. When XZX-S5 was added, the Gambia algae cells were partially deformed, changing from round to oval, and the color was darker. This phenomenon was observed in the group with the highest bacterial concentration (5×10 9 bacteria / mL -1 ) is particularly obvious.

[0099] 2. Detection of algal cell concentration

[0100] The concentration of Gambia algae cells in the experimental group and the control group was detected at 20h, 40h, 60h, 80h and 100h of culture in each group of co-culture systems. The counting method of Gambia algae cells is as follows:

[0101] 1) Carefully shake the cell culture flask to detach the attached Gambiae algae and distribute them evenly.

[0102] 2) Quickly use a pipette to aspirate 10 μL of algae solution and spread it on a glass slide.

[0103] 3) Count the Gambian algae using a counter under a 40x optical microscope Leica MA 16FA, repeat the counting three times, and convert the counting results into the number of Gambian algae cells per milliliter of solution, that is, the Gambian algae cell concentration cells / mL.

[0104] 4) Draw a growth curve of Gambia algae with the cell concentration of Gambia algae as the ordinate and the culture time as the abscissa.

[0105] The results of the test on the concentration of Gambia algae cells are as follows: Figure 3 As shown in the figure, the low concentration group (5×10 7 bacteria / mL -1 ) and medium concentration group (5×10 8 bacteria / mL -1 ) had a significant inhibitory effect on the growth of Gambia algae. However, when the concentration of XZX-S5 increased to a high concentration of 5×10 9bacteria / mL -1 When the concentration of XZX-S5 was 0.5, it showed a significant inhibitory effect (p<0.01, extremely significant difference), which started from the first 48 hours and lasted until the 96th hour. The above results show that the addition of XZX-S5 bacterial agent reduced the cell concentration of Gambia algae and had the function of inhibiting the growth and biomass of Gambia algae.

[0106] 3. Determination of photosynthetic capacity of algal cells

[0107] The total amount of chlorophyll in the algal cells of each co-culture system was determined using a chlorophyll fluorescence analyzer, Phytoplankton Analyzer (PHYTO-PAM, Walz). The specific steps are as follows: Carefully shake the co-culture system, and after even distribution, quickly transfer 3 mL of Gambia algae solution to a glass cuvette and place it in PHYTO-PAM. Open the PHYTO-PAM supporting software, record the chlorophyll parameters in the Report-window, and obtain photosynthesis data.

[0108] The test results of total chlorophyll content are as follows: Figure 4 As shown in the figure, it can be seen that within the first 48 hours (0-48h), the XZX-S5 bacterial agent significantly reduced the total chlorophyll content of Gambia algae cells. The higher the concentration, the lower the total chlorophyll content, which was significantly different from the control group (P<0.05). This phenomenon was more obvious in the last 48 hours (49-96h), and the high concentration group (5×10 9 bacteria / mL -1 ) The reduction of the total amount of chlorophyll was more than two times (P<0.01). The above results show that the addition of XZX-S5 bacterial agent reduced the total amount of chlorophyll in algal cells and had the function of reducing the photosynthetic capacity of Gambia algae.

[0109] 4. Detection of photosynthetic efficiency

[0110] The photosynthetic efficiency (PSII primary light energy conversion efficiency, F V / F M value).

[0111] The results of photosynthetic efficiency test are as follows Figure 5As shown in the figure. It can be seen that in the first 48 hours (0-48h), the addition of XZX-S5 bacterial agent significantly reduced the photosynthetic efficiency of Gambia algae cells, which was significantly different from the control group (P<0.05). In the last 48 hours (49-96h), with the increase of XZX-S5 concentration, the photosynthetic efficiency of Gambia algae cells gradually decreased, and the inhibitory effect on photosynthesis became more obvious (P<0.01). The above results show that XZX-S5 bacterial agent has the function of reducing the photosynthetic efficiency of Gambia algae cells.

[0112] 5. Detection of elemental composition of algal cells

[0113] The algal cells of each group of co-culture systems in the stationary period were collected, and the elemental spectrum composition of the Gambia algae cells in the experimental group and the control group was detected by a micro-area X-ray element analyzer (M4Tornado), which mainly included 10 main elements, namely Ti-titanium, Zn-zinc, Cu-copper, Mn-manganese, Fe-iron, Ca-calcium, K-potassium, S-sulfur, P-phosphorus and Si-silicon. The abundance of each element was expressed in cps / Ev.

[0114] The detection results of elemental spectrum composition are as follows Figure 6 As shown. It can be seen from the figure that the element copper Cu increased slightly in the experimental group, but the increase was not obvious; the elements silicon Si, zinc Zn and titanium Ti did not change much, which may be related to the material composition of the Gambia algae itself. Among the other important life elements, the elements phosphorus P, sulfur S, potassium K, calcium Ca, iron Fe and manganese Mn showed differences in abundance between the experimental group and the control group. When the XZX-S5 bacterial agent was added, the composition ratio of the elements phosphorus P, sulfur S, potassium K, calcium Ca, iron Fe and manganese Mn decreased accordingly, and there was a dose-dependent relationship. The above results show that the addition of the XZX-S5 bacterial agent changed the physiological state of the algae cells, affected the accumulation and metabolic capacity of the elements, and the XZX-S5 bacterial agent has the function of regulating the composition ratio of elements in the Gambia algae cells. Sequence Listing <110> Tsinghua University Shenzhen Graduate School Shenzhen Hengchuang Gene Technology Co., Ltd. <120> A bacterium for inhibiting the growth of Gambia algae and its application <160> 1 <210> 1 <211> 1434bp <212> DNA <213> Artificial sequence <220> <223> <400> 1 gcagctacac atgcaagtcg aacggtaaca ggaagcagct tgctgctttg ctgacgagtg 60 gcggacgggt gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg 120 gtagctaata ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat 180 cggatgtgcc cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc 240 cctagctggt ctgagaggat gaccagccac actggaactg agacacggtc cagactccta 300 cgggaggcag cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg 360 tgtatgaaga aggccttcgg gttgtaaagt actttcagcg gggaggaagg tgttgaggtt 420 aataacctca gcaattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag 480 ccgcggtaat acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag 540 gcggtctgtc aagtcggatg tgaaatcccc gggctcaacc tgggaactgc attcgaaact 600 ggcaggctag agtcttgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga 660 gatctggagg aataccggtg gcgaaggcgg ccccctggac aaagactgac gctcaggtgc 720 gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg 780 acttggaggt tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct 840 ggggagtacg gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg 900 gagcatgtgg tttaattcga tgcaacgcga agaaccttac ctactcttga catccagaga 960 acttagcaga gatgctttgg tgccttcggg aactctgaga caggtgctgc atggctgtcg 1020 tcagctcgtg ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg 1080 ttgccagcgg tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg 1140 gggatgacgt caagtcatca tggcccttac gagtagggct acacacgtgc tacaatggcg 1200 catacaaaga gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg 1260 gattggagtc tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat 1320 gccacggtga atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt 1380 tgcaaaagaa gtaggtagct taaccttcgg gagggcgcta ccacttgatc gtga 1434

Claims

1. A Gammaproteus ( Gamma proteobacterium )XZX-S5, the Gammaproteus ( Gamma proteobacterium ) The deposit number of XZX-S5 is GDMCC No. 60157.

2. The gammaproteus according to claim 1 ( Gamma proteobacterium ) Application of XZX-S5 or its bacterial agent in inhibiting the growth of Gambia algae.

3. The gammaproteus according to claim 1 ( Gamma proteobacterium ) Application of XZX-S5 or its bacterial agent in the preparation of products for inhibiting the growth of Gambian algae.

4. The gammaproteus according to claim 1 ( Gamma proteobacterium ) Application of XZX-S5 or its bacterial agent in reducing the photosynthetic efficiency of Gambia algae.

5. The gammaproteus according to claim 1 ( Gamma proteobacterium ) Application of XZX-S5 or its bacterial agent in the preparation of a product that reduces the photosynthetic efficiency of Gambia algae.

6. The use according to claim 5, characterized in that: The reduction of the photosynthetic efficiency of Gambia algae is reflected in the reduction of the total amount of chlorophyll in Gambia algae cells.

7. The gammaproteus according to claim 1 ( Gamma proteobacterium ) Application of XZX-S5 or its bacterial agent in regulating the element composition ratio in Gambia algae cells; the regulation of the element composition ratio in Gambia algae cells is reflected in reducing the content of phosphorus and / or sulfur and / or potassium and / or calcium and / or iron and / or manganese in Gambia algae cells.

8. The gammaproteus according to claim 1 ( Gamma proteobacterium )Application of XZX-S5 or its bacterial agent in the preparation of products for regulating the element composition ratio in Gambia algae cells; The regulation of the element composition ratio in the Gambian algae cells is embodied in reducing the content of phosphorus and / or sulfur and / or potassium and / or calcium and / or iron and / or manganese in the Gambian algae cells.

9. A bacterial agent, the active ingredient of which is the Gammaproteus ( Gamma proteobacterium )XZX-S5; The bacterial agent has any one of the following functions 1)-3): 1) Inhibit the growth of Gambia algae; 2) Reduce the photosynthetic efficiency of Gambiae; 3) Regulate the element composition ratio in Gambia algae cells.

10. A method for inhibiting the growth of Gambia algae, comprising: Gamma proteobacterium ) The step of co-culturing XZX-S5 or its bacterial agent with Gambiae algae.

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