A portable skull transparent processing method

Abstract

The present application discloses a portable skull transparency treatment method. The treatment method includes cutting an opening on the scalp to expose the skull; fixing the first solidifiable material to the wound around the exposed skull surface to form a groove for holding a buffer and fixing one end of a porous fixing plate; placing a shell with a small hole on the top in the skull area to be treated or placing the shell in the skull area to be treated to form a small hole on the shell; sealing the junction between the skull and the shell with a second solidifiable material, fixing the shell on the skull, and injecting and / or replacing the transparent liquid through the small hole. Through the above-mentioned embodiments, the present application can realize the portable carrying of transparent liquid in the skull, make the skull transparent for a long time, reduce the performance requirements for the transparent liquid, and reduce the risk of transparent liquid spilling and leaking during the skull transparency treatment process.

Description

Technical Field

[0001] The present application relates to the processing field, and in particular to a portable skull transparency processing method. Background Art

[0002] With the development of society, the skulls of humans and animals have a convex structure. In the process of brain science research on subcranial cortical tissue, due to the high scattering characteristics of the two layers of compact bone of the skull above the cortex sandwiching a layer of cancellous bone structure and the subcranial dura mater, arachnoid mater, and pia mater structure tissue, subcranial brain science research has become very difficult. Generally, the method of craniotomy and skull peeling is adopted to completely remove a part of the skull and cover the exposed meninges with a coverslip, or the thin skull window method is used, that is, the skull is thinned to 20-30μm, or a craniotomy glass skull window / thin skull window variant is used to process the skull for observation.

[0003] However, if the above methods are used, the skull structure will be damaged. In addition, they are all traumatic methods, and the inherent inflammatory response of the skull window cannot be avoided. The expected waiting time for treatment is too long. The thin skull window is in the range of 0.2-2mm. The surgical process of grinding the skull to 20-30μm is complicated and difficult, and requires high operation of the experimenter. In addition, due to the mobile characteristics of the skull or the movement during the operation, the skull presents a convex structure, which makes it easy for the poured liquid to spill or flow out. There is less liquid that can work, and it is inconvenient to carry, which leads to a short matching time for the liquid reagent. Therefore, the performance requirements of the transparent liquid are high, otherwise the transparent treatment effect of the skull cannot be achieved. Summary of the invention

[0004] The main technical problem solved by the present application is to provide a portable skull transparency processing method, which can be portable and perform skull transparency for a long time, reduce the performance requirements for transparent liquid, and at the same time reduce the risk of transparent liquid spilling and leaking.

[0005] In order to solve the above technical problems, the first aspect of the present application provides a portable skull transparency processing method, which includes: cutting an opening on the scalp to expose the skull; fixing the first solidifiable material to the wound around the exposed skull surface, which is used to hold a buffer and fix one end of a porous fixing plate; placing a shell with a small hole on the top in the skull area to be treated or placing the shell in the skull area to be treated and then forming a small hole on the shell; sealing the junction between the skull and the shell with a solidifiable material to fix the shell on the skull.

[0006] Different from the prior art, the beneficial effect of the present application is: by using a second solidifiable material to seal the junction between the skull and the shell, the shell is fixed on the skull area to be treated, so that the skull can be portable and transparent liquid can be carried for a long time for skull transparency. The sealing of the junction makes it difficult for the transparent liquid contained in the shell to flow out or spill, reducing the risk of transparent liquid spilling and leaking during the skull transparency treatment process, thereby allowing a larger volume of transparent liquid to contact the skull, so the performance requirements for the transparent liquid can be reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 This is a schematic diagram of the operation flow of the first embodiment of the portable skull transparent processing method of the present application;

[0008] Figure 2 This is a schematic diagram of the operation flow of the second embodiment of the portable skull transparency processing method of the present application. DETAILED DESCRIPTION

[0009] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0010] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0011] See also Figure 1 , Figure 1 This is a schematic diagram of the operation flow of the first embodiment of the portable skull transparent processing method of the present application, which includes the following steps:

[0012] S1: Make an opening in the scalp to expose the skull;

[0013] When making the skull transparent, consider saving the amount of materials needed during the experiment. If you want to obtain a skull that is easy to experiment with, the mouse skull is small and the mouse is small in size, so the mouse skull is an ideal choice. Because the biological activity of the experimental subject needs to be considered when making the skull transparent, the skull of a mouse aged 6 to 11 weeks can be taken, which is conducive to skull transparency. If the skull is a living skull, the processing method needs to be performed after anesthesia, which can improve the implementation of the plan. Mice are small in size, and anesthesia has side effects on mice, so the anesthesia time can be controlled within 30 minutes to keep the internal environment of the mouse stable.

[0014] The mouse head is shaved to expose the scalp covering the skull. An opening is cut on the scalp to expose the skull, which can facilitate subsequent operations.

[0015] S11: Wipe the periosteum on the surface of the skull so that saline, buffer or transparent liquid can directly contact the skull;

[0016] There is a layer of periosteum between the epidermis and skull of the mouse skull, which will block the direct contact between saline, buffer or transparent liquid and the skull, thus affecting the transparency of the skull. In order to achieve a better transparency of the skull, the periosteum on the surface of the skull can be wiped off with a cotton ball. If saline, buffer or transparent liquid is poured in afterwards, it can directly contact the skull, and the transparent liquid can quickly penetrate into the skull through the gaps between the skull bones, thereby reducing the time for the transparent liquid to penetrate into the skull.

[0017] S12: Wipe away the accumulated fluid on the surface of the skull and clean the surface of the skull with saline several times;

[0018] After wiping off the periosteum, there may be fluid on the surface of the skull, which may affect the transparent liquid from penetrating into the skull, thus affecting the transparency of the skull. In order to prevent the poured transparent liquid from being carried out by the fluid, the fluid on the surface of the skull can be wiped off and the surface of the skull can be cleaned by scrubbing with saline several times.

[0019] S2: fixing a first solidifiable material on the exposed skull surface around the wound to form a groove for containing a buffer and fixing one end of the porous fixing sheet;

[0020] After wiping off the periosteum and the accumulated fluid on the surface, the skull is clearly exposed. Because the skull presents a convex structure, if transparent liquid is directly poured on the skull, the calcium hydroxyapatite and collagen on the skull will affect the transparent effect. In addition, considering not affecting the internal environment of the mouse, a buffer needs to be poured in, which can offset and reduce the effect of external strong acid or strong alkali on the mouse skull to a certain extent. In order to increase the volume of the poured buffer, a groove can be constructed at the wound around the skull surface using the first coagulated material, and the groove can be fixed around the exposed skull surface to play the role of holding more buffer.

[0021] Furthermore, in order to facilitate the subsequent fixation of the mouse skull, the constructed groove can be used to fix one end of the porous fixing plate, thereby facilitating the subsequent fixation of the skull.

[0022] S3: placing a shell with a small hole on the top on the skull area to be treated or placing the shell on the skull area to be treated and then forming a small hole on the shell;

[0023] In order to facilitate the replacement of the transparent liquid contained in the shell, the shell with a small hole on the top can be placed in the skull area to be treated or the shell can be placed in the skull area to be treated and then a small hole can be formed on the shell. This makes it convenient to replace the transparent liquid later by using a syringe with a needle hole or a syringe containing the transparent liquid through the small hole.

[0024] It should be noted that the shell can be a plastic hemisphere or a metal hemisphere, and the specific details are not limited here.

[0025] S4: Use a second curable material to seal the interface between the skull and the shell, so that the shell is fixed on the skull area to be treated.

[0026] When the transparent liquid is injected, it is impossible for the transparent liquid to immediately soak into the skull. If too much is poured, it is easy to flow out. However, the small volume of the transparent liquid poured may limit the transparent effect. In order to facilitate the carrying of the transparent liquid and to make it transparent for a long time, an area is required to seal the skull area to be treated to hold the transparent liquid. Therefore, the second solidifiable material is used to seal the junction between the skull and the shell, so that the shell is fixed on the skull area to be treated. If the junction between the second solidifiable material and the shell is not sealed, the transparent liquid may leak out during the movement of the mouse or during the mobile operation of carrying. Therefore, the second solidifiable material is used to seal the junction between the first solidifiable material and the shell. Since the upper edge of the groove formed by the first solidifiable material is sealed with the junction of the lower edge of the shell, the transparent liquid contained in the groove is not easy to flow out or spill out, which can reduce the risk of the transparent liquid spilling and leaking during the skull transparent treatment process. Therefore, it can be convenient to carry, so that the transparent liquid reagent matches for a longer time, so the performance requirements for the transparent liquid can be reduced, and then the skull transparent treatment effect can be achieved.

[0027] In the process of skull transparency and treatment, there are many kinds of transparent liquids that can be used, and the transparent liquids can include optically transparent solutions and / or matching liquids. Because of the high scattering characteristics of the skull, the reflectivity of protein and fat tissue is relatively large. In order not to affect the transparency of the skull, the transparent liquid can be a water-soluble reagent with a high refractive index.

[0028] It should be noted that the first curable material can be dental cement, bone cement, or a mixture thereof, which is not specifically limited herein. If dental cement is used, its usage ratio should be adjusted, otherwise the fixing of the shell may be affected.

[0029] S51: injecting and / or sucking out the transparent liquid into the shell through the small hole to replace the transparent liquid;

[0030] During the process of making the skull transparent, the free components produced by a series of chelation reactions and hydrolysis reactions inside the skull enter the transparent liquid, making the transparent liquid turbid. In order to clean up the turbid transparent liquid, the transparent liquid can be injected or sucked out into the shell through the small hole to achieve the operation of replacing the transparent liquid, and different transparent liquids can be replaced multiple times as needed.

[0031] It should be noted that the capacity of the transparent liquid injected into the shell can be greater than 1 ml. The specific amount required can be determined based on the skull area to be treated, the size of the shell, and the volume of transparent liquid to be poured in, which can improve the feasibility of the solution.

[0032] S52: After a preset time, the shell is uncovered and the skull is fixed;

[0033] As the skull transparency treatment operation proceeds, after a preset time, the transparent liquid can penetrate into the skull. In order to improve the integrity of the solution, the shell can be opened and the skull can be fixed to achieve the skull transparency treatment operation.

[0034] The fixing method may specifically include: fixing the skull with a porous fixing plate, the porous fixing plate may include a double-hole aluminum plate, the double-hole aluminum plate may be in a strip shape, the holes at one end of the strip of the double-hole aluminum plate may be fixed to the side of the skull through a groove, and the holes at the other end may be used to screw on a fixing frame to fix the skull.

[0035] In this embodiment, a groove is constructed around the surface of the skull using a second solidifiable material to hold a buffer and fix one end of a porous fixing plate. A shell is placed on the skull area to be treated and the second solidifiable material is used to seal the junction with the shell with holes on the top, so that the shell can be fixed on the skull and the transparent liquid can be replaced. In this way, the skull can be portable and carry transparent liquid. By sealing the junction, the transparent liquid held in the groove is not easy to flow out or spill, reducing the risk of transparent liquid spilling or leaking during the skull transparency treatment process, thereby making the transparent liquid reagent match longer, thereby reducing the performance requirements for the transparent liquid, and thus achieving the skull transparency treatment effect.

[0036] See also Figure 2 , Figure 2 1 is a schematic diagram of the operation flow of the second embodiment of the portable skull transparency processing method of the present application. In addition to executing the steps of the operation flow of the first embodiment, the processing method also includes the following steps:

[0037] S6: Determine whether the interface needs to be resealed: if yes, seal the interface between the first solidifiable material and the shell with a second solidifiable material to fix the shell on the skull; if no, remove the solidified groove;

[0038] After the skull is made transparent, it can be determined whether the junction needs to be resealed for the next operation or for the survival of the mouse:

[0039] If yes, then jump to step S5, and seal the interface between the first solidifiable material and the shell with the second solidifiable material, so that the shell is fixed on the skull to facilitate the next skull transparent operation;

[0040] If not, remove the coagulated grooves to keep the mice in good condition.

[0041] S7: Scrub the skull surface with saline;

[0042] After removing the solidified groove, the mouse skull is exposed again. In order to reduce the damage of the transparent liquid or solidifiable material to the mouse, the skull surface can be repeatedly scrubbed with saline to keep the mouse skull surface clean.

[0043] S8: Suture the scalp.

[0044] After scrubbing the skull surface, a clean mouse skull was obtained. In order to minimize the impact on the mouse's subsequent survival status, the scalp was sutured so that the mouse skull was in a wrapped state.

[0045] In this embodiment, the transparent liquid contained in the shell is not easy to flow out or spill out by sealing the junction, which not only reduces the risk of transparent liquid spilling or leaking during the skull transparency treatment process, but also reduces the performance requirements for the transparent liquid. In addition, by constructing a sealed space to hold the transparent liquid, the internal space of the shell constructed by this treatment process can be repeatedly used to facilitate the next skull transparency operation. This treatment method allows mice to carry transparent liquid for a long time and the shell with a hole on the top is fixed on the surface of the skull, which makes liquid replacement simple and convenient, thereby realizing the long-term portable carrying of transparent liquid for skull transparency, that is, realizing portable, non-destructive skull bone window, non-invasive, and easy-to-operate skull transparency, thereby improving the feasibility of the solution to achieve skull transparency.

[0046] The above are only implementation methods of the present application, and are not intended to limit the patent scope of the present application. Any equivalent structural transformations made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A portable skull transparent processing method, It is characterized in that include: Make an opening in the mouse's scalp to expose the skull; Fixing a first solidifiable material to the wound around the exposed skull surface to form a groove for containing a buffer and fixing one end of a porous fixing sheet; Placing a shell with a small hole on the top in the skull region to be treated or forming the small hole on the shell after placing the shell in the skull region to be treated; Using a second curable material to seal the interface between the skull and the shell, so that the shell is fixed on the skull area to be treated; Injecting or sucking out a transparent liquid into the shell through the small hole to replace the transparent liquid; the transparent liquid includes an optically transparent solution and / or a matching liquid, and the transparent liquid is a water-soluble reagent with a high refractive index; After a preset time, uncovering the shell and fixing the skull; Among them, after the preset time, the shell is uncovered and the skull is fixed, including: the skull is fixed with the porous fixing plate, the porous fixing plate includes a double-hole aluminum plate, the double-hole aluminum plate is in a strip shape, the hole at one end of the strip of the double-hole aluminum plate is fixed to the side of the skull through the groove, and the hole at the other end is used to be screwed on the fixing frame to fix the skull.

2. The processing method according to claim 1, It is characterized in that After cutting an opening in the scalp to expose the skull, before fixing the first solidifiable material on the exposed surface of the skull around the wound to form a groove for containing a buffer and fixing one end of a porous fixing plate, the treatment method also includes: wiping the periosteum on the surface of the skull so that saline, buffer or transparent liquid is in direct contact with the skull.

3. The processing method according to claim 2, It is characterized in that After erasing the periosteum on the surface of the skull, the treatment method further comprises: Wiping the accumulated fluid from the surface of the skull; The surface of the skull was cleaned by scrubbing with the physiological saline several times.

4. The processing method according to claim 1, It is characterized in that The capacity of the transparent liquid injected into the groove is greater than 1 ml.

5. The processing method according to claim 1, It is characterized in that The skulls include skulls of mice aged 6 to 11 weeks.

6. The processing method according to claim 1, It is characterized in that The processing method also includes: determining whether the interface needs to be resealed: if so, sealing the interface between the first solidifiable material and the shell with the second solidifiable material to fix the shell on the skull; if not, removing the solidified groove.

7. The processing method according to claim 6, It is characterized in that After removing the solidified groove, the processing method further comprises: Scrub the skull surface with saline; The scalp was sutured.

Images