A sample pre-treatment device for detection and a method of using the same

Abstract

The present invention provides a sample pretreatment device for detection and a method for using the sample pretreatment device. The sample pretreatment device comprises: a guide rail, on which an initial position, a homogenization extraction position, a filtration separation position, a suction and exhalation position and a collection position are sequentially arranged; a base, used to support a fixed frame including a plurality of hole positions; the lower part of the base is connected to a bottom moving device, which is used to drive the base to move on the guide rail to realize the switching of the fixed frame between the initial position, the homogenization extraction position, the filtration separation position and the suction and exhalation position. The sample pretreatment device for detection of the present invention can realize functions such as homogenization, sample extract separation, and extract transfer, and the sample can be directly used for instrument equipment or rapid detection and analysis after processing.

Description

Technical Field

[0001] The present invention relates to the field of biochemical detection, and more particularly to a sample pre-treatment device for detection and a method for using the same. Background Art

[0002] In biochemical testing, samples usually need to be pre-treated for the normal progress of the subsequent testing process. For solids, they need to be crushed, the target extracted, solid-liquid separation and other processes; for liquids, they generally need to be diluted or extracted as needed, and liquid-liquid separation and other processes are performed. At present, these tests are generally independent steps and performed manually. The process is cumbersome and takes up a lot of time and labor in the testing process.

[0003] Therefore, there is a need in the art for improved sample preparation equipment. Summary of the invention

[0004] In order to solve the above problems, the present invention proposes a sample pre-treatment device for detection to solve the problem of complicated sample processing.

[0005] Therefore, in a first aspect, the present invention provides a sample pre-treatment device for detection, comprising:

[0006] A guide rail, wherein an initial position, a homogenization extraction position, a filtration separation position, a suction and exhalation position, and a collection position are sequentially arranged on the guide rail;

[0007] A base, used to support a fixed frame including a plurality of holes; the lower part of the base is connected to a bottom moving device, used to drive the base to move on the guide rail, so as to realize the switching of the fixed frame among the initial position, the homogenization extraction position, the filtering separation position and the suction and exhalation liquid position;

[0008] A first fixing frame receiving position is arranged at the initial position, a first container is arranged on the first fixing frame, the first container includes a stirring rod, and an end cap is arranged on the stirring rod;

[0009] The homogenizing extraction position is provided with a first vertical moving module, which includes a first vertical moving motor, a motor plate, a stirring motor fixed on the motor plate, and a bite head, wherein the first vertical moving motor drives the motor plate to move up and down, the bite head is used to bite with the end cap of the first container on the fixed frame, and the stirring motor drives the stirring rod in the first container to rotate;

[0010] The filtering and separating position is provided with a second vertical moving module, the second vertical moving module comprises a second vertical moving motor, a pushing plate and a pushing rod fixed on the pushing plate, and the second vertical moving motor drives the pushing plate to move up and down;

[0011] The suction and discharge liquid level is provided with a third vertical moving module, and the third vertical moving module includes a third vertical moving motor, a needle plate and a blowing and sucking needle fixed on the needle plate, and the third vertical moving motor drives the needle plate to move up and down, and the blowing and sucking needle is used to suck or release liquid;

[0012] The collection position is provided with a second fixing rack accommodation position, and the second fixing rack is used to place a second container of the processed sample;

[0013] The needle plate can be moved between the aspiration position and the collection position so that the needle injects the liquid therein into the second container of the processed sample.

[0014] In one embodiment, the sample pre-treatment device comprises a cleaning tank and / or a sample rack placed on the fixed frame.

[0015] In one embodiment, a filter screen is provided in the first container, and the filter screen is slidably mounted on the stirring rod, and the push rod pushes the filter screen downward when moving downward.

[0016] In one embodiment, the first container is a homogenizing cup.

[0017] In one embodiment, the second container is a test tube.

[0018] In one embodiment, the base slides on the rails.

[0019] In one embodiment, the occlusal head is provided with a ramp, a curved arc and a right-angled surface.

[0020] In one embodiment, the first container is a bottle or a tube for holding a sample.

[0021] In one embodiment, the needle plate slides between the aspiration position and the collection position.

[0022] In one embodiment, the fixing frame is used to fix the first container and the second container of different specifications. A movable mechanism is provided at the fixing position, and a spring pulls the side piece toward the center piece to fix the first container and the second container, thereby preventing the container body from rotating, vibrating and moving out of position during use.

[0023] In a second aspect, the present invention provides a sample pretreatment method for detection, the method using the sample pretreatment device for detection of the first aspect of the present invention, the method comprising:

[0024] (1) placing a first fixing frame on the base at the initial position, and placing a first container of a sample to be processed on the first fixing frame;

[0025] (2) The base moves on the guide rail to transport the fixed frame to the homogenization extraction position, the first vertical movement motor drives the motor plate to move downward, the engagement head engages with the end cap of the first container on the fixed frame, and drives the stirring shaft to move. After the stirring is completed, the engagement head and the end cap are released, and the first vertical movement motor drives the motor plate to return upward;

[0026] (3) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, the third vertical moving motor drives the needle plate downward to inject the buffer solution, and then the third vertical moving motor drives the needle plate upward to return to its original position;

[0027] (4) The base moves on the guide rail to transport the fixed frame to the filtering and separation position, the second vertical moving motor drives the push plate downward to push the filter screen part in the first container downward, and then the second vertical moving motor drives the push plate upward to return to its position;

[0028] (5) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid position, and the third vertical moving motor drives the needle plate downward to absorb the liquid, and then the third vertical moving motor drives the needle plate upward to return to its original position;

[0029] (6) Move the needle plate to a collecting position, and spit out the liquid into a second container on a second fixed frame at the collecting position.

[0030] In one embodiment, the fixing frame holds a cleaning tank or a sample holder.

[0031] In one embodiment, the base slides on the rails.

[0032] In one embodiment, the occlusal head is provided with a ramp, a curved arc and a right-angled surface.

[0033] In one embodiment, the first container is a homogenizing cup.

[0034] In one embodiment, the second container is a test tube.

[0035] In one embodiment, the base slides on the rails.

[0036] In one embodiment, the occlusal head is provided with a ramp, a curved arc and a right-angled surface.

[0037] In one embodiment, the first container is a bottle or a tube for holding a sample.

[0038] In one embodiment, the needle plate slides between the aspiration position and the collection position.

[0039] In one embodiment, the fixing frame is used to fix the first container and the second container of different specifications. A movable mechanism is provided at the fixing position, and a spring pulls the side piece toward the center piece to fix the first container and the second container, thereby preventing the container body from rotating, vibrating and moving out of position during use.

[0040] The sample pretreatment device for detection of the present invention can realize functions such as homogenization, sample extract separation, extract transfer, etc. After the treatment, the sample can be directly used for instrument equipment or rapid detection and analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The present invention is described by the following drawings

[0042] Figure 1A and 1B It is a schematic diagram of the overall structure of a sample pretreatment device for detection according to one embodiment of the present invention, showing a top view (1A) and a side view (1B).

[0043] Figures 2A-2C The present invention is a schematic structural diagram of a motor plate (2A), a needle plate (2B) and a push plate (2C) of a sample pretreatment device for detection according to one embodiment of the present invention.

[0044] Figure 3A and 3B A schematic structural diagram of a homogenizing cup (3A) and a bite head (3B) according to an embodiment of the present invention is shown.

[0045] Figure 4A and 4B A schematic structural diagram of a cleaning station according to an embodiment of the present invention is shown, which is shown as a top view (4A) and a side view (4B). DETAILED DESCRIPTION

[0046] Hereinafter, embodiments of the present invention are described with reference to the accompanying drawings. The following detailed description and the accompanying drawings are used to exemplarily illustrate the principles of the present invention, and the present invention is not limited to the preferred embodiments described. The scope of the present application is defined by the claims. The present invention is now described in detail with reference to exemplary embodiments, and some embodiments are illustrated in the accompanying drawings. The following description is made with reference to the accompanying drawings, and unless otherwise indicated, the same reference numerals in different drawings represent the same or similar elements. The schemes described in the following exemplary embodiments do not represent all schemes of the present invention. On the contrary, these schemes are merely examples of the apparatus and methods of various aspects of the present invention involved in the appended claims.

[0047] Figure 1A and 1B is a schematic diagram of the overall structure of a sample pretreatment device for detection according to one embodiment of the present invention, Figure 1A shows a top view, Figure 1B A side view is shown. The sample pretreatment device for detection comprises: a guide rail 1 and a base. The guide rail 1 is provided with an initial position 2, a homogenization extraction position 3, a filtration separation position 4, an aspiration and exhalation position 5 and a collection position 6 in sequence; the base is used to support a fixed frame 10 including a plurality of hole positions; the lower part of the base is connected to a bottom moving device for driving the base to move on the guide rail 1, and the guide rail can be similar to a railway track to enable the fixed frame 10 to switch between the sample rack initial position 2, the homogenization extraction position 3, the filtration separation position 4 and the aspiration and exhalation position 5; the base can slide on the guide rail 1.

[0048] The initial position 2 is used to place the first fixing frame 10, and the first fixing frame 10 is used to place the first container of the sample to be processed. The fixing frame 10 can be used to place a cleaning tank or a sample rack. For example, the multiple holes on the fixing frame 10 are used to place bottles or tubes for loading samples. Preferably, the fixing frame 10 can be used to fix the first container and the second container of different specifications. The fixing position is provided with a movable mechanism, and the spring pulls the side piece toward the center piece to fix the first container and the second container, and prevents the container body from rotating, vibrating, and disengaging during use. In the present invention, the movable mechanism pulls the side piece toward the center piece by a spring to fix the first container and the second container, and prevents the container body from rotating, vibrating, and disengaging during use. There are latch holes at the four corners for fixing on the base.

[0049] The homogenization extraction position 3 is provided with a first vertical movement module 31, and the first vertical movement module 31 includes a first vertical movement motor 310, a motor plate 312, a stirring motor 313 fixed on the motor plate 312, and an engagement head. The first vertical movement motor 310 drives the motor plate 312 to move up and down, and the engagement head is used to engage with the end cap of the first container on the fixed frame 10. Preferably, the engagement head is a replaceable part. In the present invention, the engagement head is provided with an inclined platform, an arc and a right-angle surface; the inclined platform and the arc facilitate the engagement of the engagement head and the end cap. When the engagement head descends and combines with the end cap, it can be gradually fitted, so that in case the position is not aligned, it can also be gradually slid and aligned to improve the engagement probability. In addition, the position of the end cap and the stirring rod below can also be corrected; the right-angle surface provides a force point to drive it to rotate when pushing.

[0050] The filtering and separating position 4 is provided with a second vertical moving module 41 , which includes a second vertical moving motor 410 , a pushing plate 411 and a pushing rod 412 fixed on the pushing plate 411 , and the second vertical moving motor 410 drives the pushing plate 411 to move up and down.

[0051] The suction and exhalation liquid level 5 is provided with a third vertical moving module 51, and the third vertical moving module 51 includes a third vertical moving motor 510, a needle plate 511 and a needle 512 fixed on the needle plate 511, and the third vertical moving motor 510 drives the needle plate 511 to move up and down; the collection position 6 is used to place a second fixed frame 61, and the second fixed frame 61 is used to place a second container of the processed sample, and the needle plate 511 can move between the suction and exhalation liquid level 5 and the collection position 6. For example, the needle plate 511 slides between the suction and exhalation liquid level 5 and the collection position 6. For example, it includes a sliding frame 7 and a horizontal motor 71.

[0052] Figures 2A-2C The figure is a schematic diagram of the structure of the motor plate, needle plate and push plate of the sample pre-treatment device for detection according to one embodiment of the present invention. Figure 2A As shown in FIG. 3 , a stirring motor 313 and a bite head 314 are fixed on the motor plate 312. Figure 2B As shown in FIG. 5 , a needle 512 is fixed on the needle plate 511, and the upper end of the needle 512 is connected to a pipeline 513. The pipeline 513 is used to pass liquid. Figure 2C As shown in FIG. 4 , a push rod 412 is fixed on the push plate 411 .

[0053] Figure 3A and 3B The schematic diagram of the structure of the homogenizing cup 8 and the engaging head 314 as the first container according to one embodiment of the present invention is shown. The homogenizing cup 8 is composed of a cup shell 81 and a stirring rod 82. The stirring rod 82 includes an end cap 821 for engaging with the engaging head 314. The end cap 821 has a partially conical structure, and there is a protruding plane on the cone. The protruding plane includes a protrusion 8211 bent in one direction, and the protrusion 8211 is used to push the stirring shaft to move. The engaging head 314 includes a recessed structure, which includes a slope 3141 for combining with the inclined surface 8212 of the end cap, and a further recessed structure for combining with the end cap protrusion, which includes a curved arc 3142 to reduce the probability of collision when combining with the end cap protrusion 8211, and a right-angle surface 3143 for pushing the protrusion 8211 to rotate. The stirring rod 82 can be combined with the cup shell 81, or can be independent of each other, but the opening of the cup shell 81 is not completely closed, and the push rod 412 can be inserted into the opening of the cup shell 81. In a preferred embodiment, the stirring rod 82 is independent of the cup shell 81, and the stirring rod 82 is placed in the cup shell 81, fixed in position by the bite head 314 during stirring, and inserted into the cup shell 81, and the push rod 412 is cylindrical and deeply inserted into the cup shell 81.

[0054] Figure 4A and 4B A schematic diagram of the structure of a cleaning tank according to an embodiment of the present invention is shown ( Figure 4A For top view and Figure 4BThe cleaning tank 9 is used to clean the sample needle after adding or extracting liquid into the homogenizing cup. When cleaning is required, the needle is inserted into the needle insertion port 91, and the liquid driving mechanism quickly spits out a large amount of liquid into the needle port. When the liquid exceeds the capacity of the needle insertion port, it enters the guide port 93 through the connecting tank 92 and is discharged through the external water receiving port 94, so that the needle is cleaned.

[0055] Example 1: Processing of solid samples.

[0056] Add 6 grams of fish and 5 milliliters of acetonitrile containing 1% acetic acid to 10 homogenizing cups respectively, put the homogenizing cups into the fixed frame, put the fixed frame on the base, then put the base into the guide rail, and start the equipment. The base moves on the guide rail to transport the fixed frame to the homogenization extraction position. The first vertical movement module includes a first vertical movement motor. The first vertical movement motor drives the motor plate downward. The bite head under the motor plate contacts the top cap on the top of the homogenizing cup. The bite head bites the top cap and drives the stirring shaft to move, and the fish is crushed. Then, the first vertical movement motor drives the motor plate upward to return to its position.

[0057] The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, and the third vertical movement module includes a third vertical movement motor, which drives the needle plate downward, and injects 5 ml of water using the needle on the needle plate. Then, the third vertical movement motor drives the needle plate upward.

[0058] The base moves on the guide rail to transport the fixed frame to the filtering and separation position, and the second vertical moving module includes a second vertical moving motor, which drives the push plate downward, and uses the push rod on the push plate to push the filter screen part in the homogenizing cup downward to 4 cm from the bottom. Then, the second vertical moving motor drives the push plate upward to return to its position.

[0059] The base moves on the guide rail, transports the fixed frame to the suction and discharge position, and the third vertical moving motor drives the needle plate downward, and uses the needle on the needle plate to absorb 6 ml of liquid. Then, the third vertical moving motor drives the needle plate upward, and the needle plate moves to the collection position, and the needle spits out 6 ml of liquid into the test tube on the fixed frame at the collection position. The liquid is the test liquid required for the next step of analysis.

[0060] Example 2: Processing of liquid samples.

[0061] Add 10 ml of milk and 5 ml of an aqueous solution containing 5% trifluoroacetic acid into 10 homogenizing cups respectively, put the homogenizing cups into a fixed frame, put the fixed frame on the base, then put it into the guide rail, and start the equipment. The base moves on the guide rail to transport the fixed frame to the homogenization extraction position. The first vertical movement module includes a first vertical movement motor, which drives the motor plate downward. The bite head under the motor plate contacts the top cap on the top of the homogenizing cup, and the bite head bites the top cap and drives the stirring shaft to move. Then, the first vertical movement motor drives the motor plate upward to return to its position.

[0062] The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, and the third vertical movement module includes a third vertical movement motor, which drives the needle plate downward, and injects 5 ml of pH 10 HEPES buffer using the needle on the needle plate. Then, the third vertical movement motor drives the needle plate upward to return to its position.

[0063] The base moves on the guide rail to transport the fixed frame to the filtering and separating position, and the second vertical moving module includes a second vertical moving motor, which drives the push plate downward, and uses the push rod on the push plate to push the filter screen part in the homogenizing cup downward to 5 cm from the bottom. Then, the second vertical moving motor drives the push plate upward to return to its position.

[0064] The base moves on the guide rail, transports the fixed frame to the suction and discharge position, and the third vertical moving motor drives the needle plate downward, and uses the needle on the needle plate to absorb 6 ml of liquid. Then, the third vertical moving motor drives the needle plate upward, and the needle plate moves to the collection position, and the needle spits out 6 ml of liquid into the test tube on the fixed frame at the collection position. The liquid is the test liquid required for the next step of analysis.

Claims

1. A sample pretreatment device for detection, comprising: A guide rail, wherein an initial position, a homogenization extraction position, a filtration separation position, a suction and exhalation position, and a collection position are sequentially arranged on the guide rail; A base, used to support a fixed frame including a plurality of holes; the lower part of the base is connected to a bottom moving device, used to drive the base to move on the guide rail, so as to realize the switching of the fixed frame among the initial position, the homogenization extraction position, the filtration separation position and the suction and exhalation liquid position; A first fixing frame receiving position is arranged at the initial position, a first container is arranged on the first fixing frame, the first container includes a stirring rod, and an end cap is arranged on the stirring rod; The homogenizing extraction position is provided with a first vertical moving module, which includes a first vertical moving motor, a motor plate, a stirring motor fixed on the motor plate, and a bite head, wherein the first vertical moving motor drives the motor plate to move up and down, the bite head is used to bite with the end cap of the first container on the fixed frame, and the stirring motor drives the stirring rod in the first container to rotate; The filtering and separating position is provided with a second vertical moving module, the second vertical moving module comprises a second vertical moving motor, a pushing plate and a pushing rod fixed on the pushing plate, and the second vertical moving motor drives the pushing plate to move up and down; The suction and discharge liquid level is provided with a third vertical moving module, and the third vertical moving module includes a third vertical moving motor, a needle plate and a blowing and sucking needle fixed on the needle plate, and the third vertical moving motor drives the needle plate to move up and down, and the blowing and sucking needle is used to suck or release liquid; The collection position is provided with a second fixing rack accommodation position, and the second fixing rack is used to place a second container of the processed sample; The needle plate moves between the aspiration position and the collection position so that the aspiration needle injects the liquid therein into the second container of the processed sample. 2 . The sample pre-processing device according to claim 1 , comprising a cleaning tank and / or a sample rack placed on the fixed frame. 3 .

3. The sample pretreatment device according to claim 1 or 2, wherein the first container has a filter screen, the filter screen is slidably mounted on the stirring rod, and the push rod pushes the filter screen downward when moving downward. The sample pretreatment device according to claim 1 or 2, wherein the base slides on the guide rail. 5 . The sample pretreatment device according to claim 1 or 2 , wherein the first container is a bottle or a tube for loading a sample. The sample pretreatment device according to claim 1 or 2, wherein the first container is a homogenizing cup.

7. The sample pretreatment device according to claim 1 or 2, wherein the occlusal head is provided with an inclined platform, an arc and a right-angle surface.

8. The sample pretreatment device according to claim 1 or 2, wherein the needle plate slides between the aspiration and dispensing position and the collection position.

9. According to the sample pretreatment equipment according to claim 1 or 2, the fixing frame is used to fix the first container and the second container of different specifications, and a movable mechanism is provided at the fixing position, and the side piece is pulled toward the center piece by a spring to fix the first container and the second container, thereby preventing the container body from rotating, vibrating and moving out of position during use.

10. A sample pretreatment method for detection, the method using the sample pretreatment device according to any one of claims 1 to 9, the method comprising: (1) placing a first fixing frame on the base at the initial position, and placing a first container of a sample to be processed on the first fixing frame; (2) The base moves on the guide rail to transport the fixed frame to the homogenization extraction position, the first vertical movement motor drives the motor plate to move downward, the bite head bites with the end cap of the first container on the fixed frame, and drives the stirring shaft to move. After the stirring is completed, the bite head and the end cap are released, and the first vertical movement motor drives the motor plate to return to its original position upward; (3) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, the third vertical moving motor drives the needle plate downward to inject the buffer solution, and then the third vertical moving motor drives the needle plate upward to return to its original position; (4) The base moves on the guide rail to transport the fixed frame to the filtering and separation position, the second vertical moving motor drives the push plate downward to push the filter screen part in the first container downward, and then the second vertical moving motor drives the push plate upward to return to its position; (5) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid position, and the third vertical moving motor drives the needle plate downward to absorb the liquid, and then the third vertical moving motor drives the needle plate upward to return to its original position; (6) Move the needle plate to a collecting position, and spit out the liquid into a second container on a second fixed frame at the collecting position.

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