An apparatus for rapidly evaluating the state of airborne bacterial contamination

Abstract

The present invention discloses a device for rapidly evaluating the state of air bacterial contamination, comprising a target particle separation and concentration system for separating target particles in the sampling air flow and performing concentration and liquefaction treatment; a sample delivery and lysis system including a quantitative pipette connected to the target particle separation and concentration system, and an ultrasonic lysis reaction cell connected to the quantitative pipette through a pipeline, where the liquefied sample undergoes lysis and luminescence reaction in the ultrasonic lysis reaction cell; a bioluminescence detection system including a light receiving component for collecting the light intensity of the ultrasonic lysis reaction cell and a signal processing device connected to the light receiving component. The entire device integrates sampling and bioluminescence detection, and can rapidly evaluate the state of air bacterial contamination. The present invention can be widely applied to the rapid analysis of the bacterial components of bioaerosols, and has application prospects in the fields of atmospheric pollution research and monitoring, indoor air microbial pollution analysis, research and monitoring of airborne diseases, etc.

Description

Technical Field

[0001] The present invention relates to a device for pollution assessment, and particularly to a device for rapidly assessing the state of air bacterial pollution. Background Art

[0002] In the study of indoor air microbial pollution, according to different research purposes, it is often necessary to separate and collect microbial aerosol particles within a certain particle size range into a specific liquid and then perform off-line analysis. The traditional classical methods for detecting and analyzing air microorganisms are: (1) using an air microbial sampler to collect air microorganisms onto a medium, namely solid nutrient agar, semi-solid nutrient agar, liquid medium, etc.; (2) there are various quantitative analysis methods, mainly culture analysis; (3) qualitative analysis mainly includes biochemical analysis, nucleic acid detection analysis, sequencing analysis, etc. Whether it is quantitative analysis or qualitative analysis of air microorganisms, it is time-consuming and laborious, and also uses a lot of consumables.

[0003] Currently, whether it is for detecting microorganisms in the atmosphere or in indoor air, it is mainly through sampling and laboratory culture of samples to analyze the concentration, which generally takes 48 - 72 hours, is both laborious and time-consuming, and cannot meet the needs of real-time detection and on-line monitoring. The ATP bioluminescence technology uses ATP in living microbial cells as the target for bioluminescence testing, measures the luminescence intensity through a bioluminescence reaction, and further estimates the concentration of live bacteria in the sample. The ATP bioluminescence detection technology can complete the detection of the concentration of live microorganisms in the sample within ≤5 minutes; this technology has been applied and studied abroad for more than 30 years, and the ATP bioluminescence technology has become a common method for evaluating surface bacterial pollution. However, it is rarely used for detecting and evaluating air bacterial pollution, especially there is currently no technology and device that integrates sampling and detection. Summary of the Invention

[0004] Aiming at the above problems, the purpose of the present invention is to provide a device for rapidly assessing the state of air bacterial pollution that integrates sampling and bioluminescence detection.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions: A device for rapidly assessing the state of air bacterial pollution, comprising:

[0006] A target particle separation and concentration system for separating target particles from the sampling air flow and performing concentration and liquefaction treatment;

[0007] A sample delivery and lysis system, including a quantitative pipette connected to the target particle separation and concentration system, and an ultrasonic lysis reaction cell connected to the quantitative pipette through a pipeline. The quantitative pipette is used to deliver the liquefied sample into the ultrasonic lysis reaction cell, and the liquefied sample undergoes lysis and luminescence reaction in the ultrasonic lysis reaction cell;

[0008] A bioluminescence detection system, comprising a light receiving component for collecting the light intensity of the ultrasonic lysis reaction cell, and a signal processing device connected to the light receiving component, wherein the signal processing device is used to convert the light intensity signal transmitted by the light receiving component into the concentration of target particles.

[0009] Preferably, the target particle separation and concentration system comprises:

[0010] A target particle separator for separating and concentrating the sampled air flow;

[0011] A sample collector, in which a liquid medium is placed. The sample collector is connected to the target particle separator through a first pipeline. One end of the first pipeline communicates with the target particle separator, and the other end of the first pipeline extends into the liquid medium of the sample collector. The sample collector is connected to the quantitative pipette through a second pipeline. One end of the second pipeline extends into the liquid medium of the sample collector, and the other end of the second pipeline communicates with the quantitative pipette;

[0012] An exhaust fan, which is connected to the target particle separator and the sample collector respectively through a third pipeline.

[0013] Preferably, the target particle separator comprises a top cover, an air inlet cover, a first-stage deflector, a first-stage impact plate, a second-stage deflector, a sampling cavity, a second-stage collection cavity, a second-stage collection plate, a second-stage exhaust hole plate, an exhaust port, a bottom plate and a sample collection port;

[0014] A sampling port is left at the top of the air inlet cover, and the top cover is connected to the sampling port of the air inlet cover. The first-stage deflector and the second-stage impact plate are arranged at intervals up and down in the air inlet cover. The bottom of the air inlet cover communicates with the top of the sampling cavity. A plurality of first diversion holes are arranged on the first-stage deflector, a depression is arranged on the top surface of the second-stage impact plate directly below the plurality of first diversion holes, and a plurality of through holes are arranged on the second-stage impact plate outside the depression;

[0015] The second-stage deflector plate and the second-stage exhaust hole plate are arranged at an upper and lower interval in the sampling cavity. A plurality of second deflector holes are provided on the second deflector plate. The second-stage collection cavity is arranged between the second-stage deflector plate and the second-stage exhaust hole plate. There is an annular gap between the second-stage collection cavity and the sampling cavity. The second-stage collection cavity includes a collection cavity section, a conical cavity section, and a straight cylinder cavity section that are connected in sequence from top to bottom. The straight cylinder cavity section is fixed on the second-stage exhaust hole plate, and the lower part of the straight cylinder cavity section penetrates the second-stage exhaust hole plate. The second-stage collection plate is arranged in the collection cavity section. A plurality of collection holes are opened on the second-stage collection plate, and the collection holes correspond to the second deflector holes one by one up and down.

[0016] The exhaust port is vertically and penetratingly arranged on the side wall of the sampling cavity where the second-stage exhaust hole plate is located. The bottom plate is fixedly arranged at the bottom of the sampling cavity. The sample collection port is fixedly arranged on the bottom plate. The upper part of the sample collection port extends into the sampling cavity and corresponds to the lower part of the straight cylinder cavity section. The exhaust port is connected to the exhaust fan through a third pipeline, and the lower part of the sample collection port is connected to the sample collector through a first pipeline.

[0017] Preferably, a plurality of the second deflector holes on the second deflector plate are directly below the depression on the first-stage impact plate.

[0018] The aperture of the through hole on the first-stage impact plate is larger than the aperture of the first deflector hole on the first-stage deflector plate, and the aperture of the second deflector hole is smaller than the aperture of the first deflector hole.

[0019] Preferably, the ultrasonic lysis reactor includes an ultrasonic lysis tank, a lysis reaction tank inserted in the ultrasonic lysis tank, and a light transmission hole opened on the side wall of the ultrasonic lysis tank. A lysis agent is placed in the lysis reaction tank. The light receiving assembly collects the light intensity of the lysis reaction tank through the light transmission hole.

[0020] Preferably, the ultrasonic lysis tank is a tank body made of metal or plastic, and the volume is 20 m l to 30 m l.

[0021] Preferably, the light receiving assembly includes a photomultiplier tube for collecting the light intensity of the ultrasonic lysis reactor, and the photomultiplier tube is connected to the signal processing device.

[0022] Preferably, the signal processing device is provided with a serial port and a USB interface connected to a computer for exporting the data of sampling detection.

[0023] It also includes an electric control system for controlling the operation of the target particle separation and concentration system, the sample transportation and lysis system, and the bioluminescence detection system.

[0024] Preferably, the target particle separation concentrator, the sample collector and the exhaust fan are integrally arranged in the first cabinet, and a first cabinet door is left on the first cabinet.

[0025] A power pump is arranged on the third pipeline between the exhaust fan and the target particle separation concentrator.

[0026] The sample collector uses a collection bottle, and the liquid medium therein is 20 ml.

[0027] Preferably, the quantitative pipette and the ultrasonic lysis reaction cell are integrally arranged in the second cabinet, and a second cabinet door is left on the second cabinet.

[0028] The present invention adopts the above technical solutions, and has the following advantages:

[0029] 1. The present invention separates the target in the sampling air flow through the target particle separation and concentration system, and performs concentration and liquefaction treatment. In the sample transportation and lysis system, lysis and luminescence reactions occur. The light receiving component in the bioluminescence detection system collects the light intensity, and transmits the light intensity signal to the signal processing device. The signal processing device converts the light intensity signal into the target particle concentration, that is, the bacterial concentration in the collected sample. The whole device integrates sampling and bioluminescence detection, and can detect the sample in situ and quickly evaluate the air bacterial pollution status. The present invention can be widely applied to the rapid analysis of the bacterial components of bioaerosols, and has application prospects in the fields of atmospheric pollution research and monitoring, indoor air microbial pollution analysis, research and monitoring of airborne diseases, etc.

[0030] 2. The target particle separation and concentration system of the present invention includes a target particle separation concentrator, a sample collector and an exhaust fan. The exhaust fan mainly provides the power for the air flow. The target particle separation concentrator adopts the principle of virtual impact, which can remove large particles and small particles in the sample, retain particles with a particle size (D) range of 0.5 μm ≤ D ≤ 10 μm, and can concentrate the target particles to be collected into an air flow of 5 L / min - 15 L / min; the target particle separation concentrator can also adopt other samplers that can collect air flow for separation and concentration, and its sampling flow rate is not limited; the concentrated air flow containing target particles impacts into the liquid medium (the medium includes sterile water, PBS buffer solution, 0.9% normal saline, etc.) in the sample collector to realize the liquefaction treatment of the sample.

[0031] 3. The sample collected by the present invention flows in a fully enclosed path formed by the target particle separation concentrator, the sample collector, the quantitative pipetting system and the lysis and luminescence reaction system, which can avoid possible human operation pollution and improve the evaluation accuracy.

[0032] 4. The sample collector, reaction cell, pipeline, lysis solution, enzyme, etc. of the present invention are all disposable consumables, which are replaced during different detection and evaluation processes to avoid cross-contamination.

[0033] 5. The operation of the entire device of the present invention is controlled by an electric control system to achieve intelligent sampling control, reduce the interference of human factors, and obtain more objective results. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0035] Figure 2 is a schematic diagram of the structure of the target particle separation and concentration device of the present invention;

[0036] In the figure, 1. Target particle separation and concentration system; 10. Cabinet; 11. Target particle separation and concentrator; 12. Sample collector; 13. Exhaust fan; among them, 111. Top cover, 112. Air intake cover, 113. First-stage deflector, 114. First-stage impact plate, 115. Second-stage deflector; 116. Sampling cavity, 117. Second-stage collection cavity, 118. Second-stage collection plate, 119. Second-stage exhaust hole plate, 1101. Exhaust port, 1102. Bottom plate, 1103. Sample collection port;

[0037] 2. Sample transportation and lysis system; 20. Cabinet; 21. Quantitative pipette; 22. Ultrasonic lysis reaction cell; among them, 221. Ultrasonic lysis tank; 222. Lysis reaction cell; 223. Light transmission hole;

[0038] 3. Bioluminescence detection system; 31. Light receiving component; 311. Photomultiplier tube; 32. Signal processing device. DETAILED DESCRIPTION OF THE INVENTION

[0039] The present invention will be described in detail below with reference to the drawings and embodiments. However, it should be understood that the provision of the drawings is only for a better understanding of the present invention, and they should not be construed as a limitation to the present invention.

[0040] As Figure 1 shown, the present invention provides a device for quickly evaluating the air bacterial pollution status, and the device includes:

[0041] A target particle separation and concentration system 1 for separating target particles from the sampling air flow and performing concentration and liquefaction treatment;

[0042] The sample delivery and lysis system 2 includes a quantitative pipette 21 connected to the target particle separation and concentration system 1, and an ultrasonic lysis reaction cell 22 connected to the quantitative pipette 21 through a pipeline. The quantitative pipette 21 is used to deliver the liquefied sample into the ultrasonic lysis reaction cell 22, and the liquefied sample undergoes lysis and luminescence reactions in the ultrasonic lysis reaction cell 22;

[0043] The bioluminescence detection system 3 includes a light receiving component 31 for collecting the light intensity of the ultrasonic lysis reaction cell 22, and a signal processing device 32 connected to the light receiving component 31. The signal processing device 32 is used to convert the light intensity signal transmitted by the light receiving component 31 into the concentration of target particles.

[0044] When the present invention is in use: The target particle separation and concentration system 1 separates the target particles from the sampling air flow (aerosol particles in the atmosphere), and concentrates and liquefies the particles with a particle size of 0.5 μm to 10 μm to convert them into a liquid sample. The quantitative pipette 21 delivers the liquefied sample into the ultrasonic lysis reaction cell 22, and the target particles in the hydraulic sample undergo lysis and luminescence reactions in the ultrasonic lysis reaction cell 22; The light receiving component 31 collects the light intensity, and the signal processing device 32 processes the light intensity signal transmitted by the light receiving component 31 and converts it into the concentration of target particles, which is the concentration of bacteria in the collected sample.

[0045] In one embodiment, the target particle separation and concentration system 1 includes: a target particle separator 11 for separating and concentrating the sampling air flow; a sample collector 12 in which a liquid medium is placed. The sample collector 12 is connected to the target particle separator 11 through a first pipeline. One end of the first pipeline communicates with the target particle separator 11, and the other end of the first pipeline extends into the liquid medium of the sample collector 12. The sample collector 12 is connected to the quantitative pipette 21 through a second pipeline. One end of the second pipeline extends into the liquid medium of the sample collector 12, and the other end of the second pipeline communicates with the quantitative pipette 21; an exhaust fan 13 is connected to the target particle separator 11 and the sample collector 12 through a third pipeline respectively. When in use, the exhaust fan 13 is started, the sampling air flow is sucked into the target particle separator 11, the non-target particles separated in the target particle separator 11 enter the third pipeline along with the air flow and are discharged through the exhaust fan 13. The concentrated target particles enter the sample collector 12. A liquid medium is placed in the sample collector 12, and the target particle gas dissolves into the liquid medium to complete the liquefaction process of the target particles and form a liquid sample; Since the exhaust fan 13 sucks away the gas in the sample collector 12 in advance, the target particle gas is prevented from mixing with other gases.

[0046] In one embodiment, as Figure 2As shown in the figure, the target particle separation concentrator 11 includes a top cover 111, an air inlet cover 112, a first-stage deflector 113, a first-stage impact plate 114, a second-stage deflector 115, a sampling cavity 116, a second-stage collection cavity 117, a second-stage collection plate 118, a second-stage exhaust hole plate 119, an exhaust port 1101, a bottom plate 1102, and a sample collection port 1103;

[0047] The top of the air inlet cover 112 has a sampling port, the top cover 111 is connected to the sampling port of the air inlet cover 112, the first-stage deflector 113 and the first-stage impact plate 114 are arranged at intervals up and down in the air inlet cover 112, and the bottom of the air inlet cover 112 communicates with the top of the sampling cavity 116; a number of first diversion holes are provided on the first-stage deflector 113, a depression is provided on the top surface of the first-stage impact plate 114 directly below the number of first diversion holes, and a number of through holes are provided on the first-stage impact plate 114 outside the depression;

[0048] The second-stage deflector 115 and the second-stage exhaust hole plate 119 are arranged at intervals up and down in the sampling cavity 116, a number of second diversion holes are provided on the second deflector 115, the second-stage collection cavity 117 is arranged between the second-stage deflector 115 and the second-stage exhaust hole plate 119, an annular gap is left between the second-stage collection cavity 117 and the sampling cavity 116, the second-stage collection cavity 117 includes a collection cavity section 1171, a conical cavity section 1172, and a straight cylinder cavity section 1173 that are connected in sequence from top to bottom, the straight cylinder cavity section 1173 is fixed on the second-stage exhaust hole plate 119, and the lower part of the straight cylinder cavity section 1173 penetrates through the second-stage exhaust hole plate 119; the second-stage collection plate 118 is arranged in the collection cavity section 1171, a number of collection holes are provided on the second-stage collection plate 118, and the collection holes correspond to the second diversion holes one by one up and down;

[0049] The exhaust port 1101 is vertically and penetratively arranged on the side wall of the sampling cavity 116 below the second-stage exhaust hole plate 119; the bottom plate 1102 is fixedly arranged at the bottom of the sampling cavity 116; the sample collection port 1103 is fixedly arranged on the bottom plate 1102, the upper part of the sample collection port 1103 extends into the sampling cavity 116 and corresponds to the lower part of the straight cylinder cavity section 1173; the exhaust port 1101 is connected to the exhaust fan 13 through a third pipeline, and the lower part of the sample collection port 103 is connected to the sample collector 12 through a first pipeline.

[0050] When the target particle separation concentrator 11 in this embodiment is in use, the top cover 111 is opened, and the particles enter the air inlet cover 112 from the sampling port along with the total sampling airflow. After passing through a plurality of first guide holes on the first-stage guide plate 113, the large particles directly impact the depression on the first-stage impact plate 114, so that most of the particles larger than 10 μm are blocked; the small particles pass through the through holes on the first-stage impact plate 114 along with the airflow, and after passing through the second guide holes on the second-stage guide plate 115, the particles larger than 0.5 μm directly pass through the collection hole located directly below the second guide hole under the action of inertia and enter the second-stage collection chamber 117. In this way, in the second-stage collection, the particles are separated and concentrated into the second-stage collection chamber 117. Most of the particles in the collection cavity 117 are particles with a particle size of 0.5 to 10 μm; while particles smaller than 0.5 μm continue to move downward through the annular gap between the second-stage collection cavity 117 and the sampling cavity 116 under the action of the airflow, enter the space between the second-stage exhaust hole plate 119 and the bottom plate 1102 through the small holes of the second-stage exhaust hole plate 119, and are discharged through the exhaust port 1101 together with the airflow; particles coming out of the straight cylinder cavity section 1173 of the second-stage collection cavity 117, particles with a size of 0.5 to 10 μm are collected through the sample collection port 1103, and particles smaller than 0.5 μm are discharged from the exhaust port 1101 along with the airflow. Among them, the airflow in the exhaust port 1101 is 193 L / min, the airflow in the sample collection port 1103 is 7 L / min, and the total sampling airflow (the flow rate entering the air inlet cover through the sampling port) is 200 L / min. The target particle separation concentrator in this embodiment can collect some low-concentration ambient air, which can increase the microbial concentration of the collected samples in a shorter period of time and improve the detection rate of subsequent tests; it is particularly beneficial for on-site rapid detection of microbial pollution concentrations in some indoor ambient air and pre-assessment of hazards.

[0051] In one embodiment, the target particle separation concentrator 11 may also use other samplers that can collect airflow for separation and concentration, and the sampling flow rate is not limited; for example, a sampler that separates particles based on the virtual impact principle and can concentrate particles of target particle size.

[0052] In one embodiment, a plurality of second guide holes on the second guide plate 115 are located directly below the depression on the first-stage impact plate 14. Thus, when the particles pass through the through holes on the first-stage impact plate 114, the larger particles (such as particles larger than 10 μm) directly impact the second-stage guide plate 115 under the action of inertia, thereby being retained. The smaller particles pass through the second guide holes on the second-stage guide plate 115 with the airflow, which can effectively remove the large particles that are not blocked on the first-stage impact plate 114, thereby improving the purity of the collected target particles of 0.5 to 10 μm.

[0053] In one embodiment, the aperture diameter of the through holes on the first-stage impact plate 114 is larger than that of the first diversion holes on the first-stage diversion plate 113, and the aperture diameter of the second diversion holes is smaller than that of the first diversion holes.

[0054] In a preferred embodiment, the target particle separation concentrator 11, the sample collector 12, and the exhaust fan 13 are integrally arranged in a cabinet 10. There is a cabinet door on the cabinet 10 to facilitate the replacement of the sample collector 12 and the pipelines connected between the components, and to avoid cross-contamination.

[0055] In one embodiment, the ultrasonic lysis reaction cell 22 includes an ultrasonic lysis tank 221, a lysis reaction cell 222 inserted in the ultrasonic lysis tank, and a light-transmitting hole 223 opened on the side wall of the ultrasonic lysis tank 221. A lysis agent is placed in the lysis reaction cell 222; the light receiving assembly 31 collects the light intensity of the lysis reaction cell 222 through the light-transmitting hole 223. During use, there are ultrasonic waves in the ultrasonic lysis tank 221, and the lysis reaction cell 222 is inserted therein. The ultrasonic action can improve the bacterial lysis degree in the lysis reaction cell 222, shorten the lysis time, and improve the working efficiency.

[0056] In a preferred embodiment, the ultrasonic lysis tank 221 is a tank made of metal or plastic, and its volume can be between 15 ml and 30 ml.

[0057] In a preferred embodiment, the quantitative pipette 21 and the ultrasonic lysis reaction cell 22 are integrally arranged in a cabinet 20. There is a cabinet door on the cabinet 20 to facilitate the replacement of the ultrasonic lysis reaction cell 22 and the pipelines connected between the components, and to avoid cross-contamination.

[0058] In one embodiment, the light receiving assembly 31 includes a photomultiplier tube 311 for collecting the light intensity in the ultrasonic lysis reaction cell 22, and the photomultiplier tube 311 is connected to the signal processing device 32.

[0059] In a preferred embodiment, a power pump (not shown in the figure) is provided on the third pipeline between the exhaust fan 13 and the target particle separation concentrator 11 to facilitate the control of the sampling flow rate.

[0060] In a preferred embodiment, the sample collector 12 can adopt a cylindrical or other-shaped collection bottle, and the liquid sampling medium therein is 20 ml.

[0061] In a preferred embodiment, the particle size range of the target particles is 0.5 μm ≤ D ≤ 10 μm, the ultimate concentrated sampling flow rate is 5 L / min to 15 L / min, and the air flow rate carrying the target particles between the target particle separation concentration system 1 and the sample delivery lysis system 2 is 5 L / min to 15 L / min.

[0062] In a preferred embodiment, a serial port and a USB interface connected to a computer are provided on the signal processing device 32 for exporting the sampled and detected data.

[0063] In a preferred embodiment, the present invention further includes an electronic control system for controlling the operation of the target particle separation and concentration system 1, the sample delivery and lysis system 2, and the bioluminescence detection system 3, so as to facilitate the unified regulation of each system.

[0064] The present invention is only described by the above embodiments, and the structures, installation positions and connections of each component can be changed. Based on the technical solution of the present invention, any improvement or equivalent transformation made to individual components according to the principle of the present invention should not be excluded from the protection scope of the present invention.

Claims

1. A device for quickly evaluating the state of air bacterial contamination, characterized in that, Comprising: A target particle separation and concentration system for separating target particles from a sampled gas stream and performing concentration and liquefaction treatment; A sample delivery and lysis system, including a quantitative pipette connected to the target particle separation and concentration system, an ultrasonic lysis reaction cell connected to the quantitative pipette through a pipeline, the quantitative pipette being used for delivering a liquefied sample into the ultrasonic lysis reaction cell, and the liquefied sample performing lysis and luminescence reactions in the ultrasonic lysis reaction cell; A bioluminescence detection system, including a light receiving component for collecting the light intensity of the ultrasonic lysis reaction cell, and a signal processing device connected to the light receiving component, the signal processing device being used for converting the light intensity signal transmitted by the light receiving component into the concentration of target particles; The target particle separation and concentration system includes: A target particle separator for separating and concentrating a sampled gas stream; A sample collector with a liquid medium placed therein, the sample collector being connected to the target particle separator through a first pipeline, one end of the first pipeline communicating with the target particle separator, the other end of the first pipeline extending into the liquid medium of the sample collector, the sample collector being connected to the quantitative pipette through a second pipeline, one end of the second pipeline extending into the liquid medium of the sample collector, and the other end of the second pipeline communicating with the quantitative pipette; An exhaust fan connected to the target particle separator and the sample collector respectively through a third pipeline.

2. The device for rapidly evaluating the air bacterial pollution status according to claim 1, wherein: The target particle separator includes a top cover, an air inlet cover, a first-stage deflector, a first-stage impact plate, a second-stage deflector, a sampling cavity, a second-stage collection cavity, a second-stage collection plate, a second-stage exhaust hole plate, an exhaust port, a bottom plate, and a sample collection port; A sampling port is left at the top of the air inlet cover, the top cover is connected to the sampling port of the air inlet cover, the first-stage deflector and the second-stage impact plate are arranged at intervals up and down in the air inlet cover, the bottom of the air inlet cover communicates with the top of the sampling cavity, a plurality of first diversion holes are provided on the first-stage deflector, a depression is provided on the top surface of the second-stage impact plate directly below a plurality of the first diversion holes, and a plurality of through holes are provided on the second-stage impact plate outside the depression; The second-stage deflector and the second-stage exhaust hole plate are arranged at intervals up and down in the sampling cavity, and a plurality of second diversion holes are provided on the second-stage deflector; the second-stage collection cavity is arranged between the second-stage deflector and the second-stage exhaust hole plate, a ring gap is left between the second-stage collection cavity and the sampling cavity, the second-stage collection cavity includes a collection cavity section, a conical cavity section, and a straight cylinder cavity section that are connected in sequence from top to bottom, the straight cylinder cavity section is fixed on the second-stage exhaust hole plate, and the lower part of the straight cylinder cavity section penetrates through the second-stage exhaust hole plate; The second-stage collection plate is arranged in the collection cavity section, and a plurality of collection holes are provided on the second-stage collection plate, and the collection holes correspond to the second diversion holes one by one up and down; The exhaust port is vertically and penetratingly arranged on the side wall of the sampling cavity located at the second-stage exhaust orifice plate; the bottom plate is fixedly arranged at the bottom of the sampling cavity; the sample collection port is fixedly arranged on the bottom plate, and the upper part of the sample collection port extends into the sampling cavity and corresponds to the lower position of the straight cylinder cavity section; The exhaust port is connected to the exhaust fan through a third pipeline, and the lower part of the sample collection port is connected to the sample collector through a first pipeline.

3. The device for rapidly evaluating the air bacterial pollution status according to claim 2, wherein: A plurality of the second diversion holes on the second-stage diversion plate are directly below the recess on the first-stage impact plate; The aperture of the through hole on the first-stage impact plate is larger than the aperture of the first diversion hole on the first-stage diversion plate, and the aperture of the second diversion hole is smaller than the aperture of the first diversion hole.

4. The device for rapidly evaluating the air bacterial pollution status according to claim 1, wherein: The ultrasonic lysis reaction cell includes an ultrasonic lysis tank, a lysis reaction cell inserted in the ultrasonic lysis tank, and a light-transmitting hole opened on the side wall of the ultrasonic lysis tank. A lysis agent is placed in the ultrasonic lysis reaction cell; the light receiving component collects the light intensity of the ultrasonic lysis reaction cell through the light-transmitting hole.

5. The device for rapidly evaluating the air bacterial pollution status according to claim 4, wherein: The ultrasonic lysis tank is a tank body made of metal or plastic, and its volume is between 15 ml and 30 ml.

6. The device for rapidly evaluating the air bacterial pollution status according to claim 1, wherein: The light receiving component includes a photomultiplier tube for collecting the light intensity inside the ultrasonic lysis reaction cell, and the photomultiplier tube is connected to the signal processing device.

7. The device for rapidly evaluating the state of airborne bacterial contamination according to claim 1, wherein: The signal processing device is provided with a serial port and a USB interface connected to a computer for exporting the data of sampling detection; It further includes an electric control system for controlling the operation of the target particle separation and concentration system, the sample transportation and lysis system, and the bioluminescence detection system.

8. The device for rapidly evaluating the air bacterial pollution status according to claim 1, wherein: The target particle separator, the sample collector, and the exhaust fan are integrally arranged in a first cabinet, and a first cabinet door is left on the first cabinet; A power pump is arranged on the third pipeline between the exhaust fan and the target particle separator; The sample collector adopts a collection bottle, and the liquid medium therein is 20 ml.

9. The device for rapidly evaluating the air bacterial pollution status according to claim 1, wherein: The quantitative pipette and the ultrasonic lysis reaction cell are integrally arranged in a second cabinet, and a second cabinet door is left on the second cabinet.

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