Incubation Tank Assembly and Detection Method of Chemiluminescent Immunoassay Analyzer

Abstract

The present invention belongs to the technical field of chemiluminescence immunoassay analyzers, and provides a incubation tank assembly for a chemiluminescence immunoassay analyzer. By arranging a linear channel inside the incubation tank, different operation positions communicating with the linear channel are arranged along the direction of the linear channel on the upper surface of the incubation tank, a driving mechanism is arranged on one side of the linear channel, and then a chip cassette is arranged. A disposable test reagent is sealed in the chip cassette, and then the chip cassette is driven by the driving mechanism to travel along the linear channel, thereby effectively avoiding the occurrence of the problem of the expiration date after opening the bottle and the operation of the "calibration" process, so that the detection process of the chemiluminescence immunoassay analyzer equipped with this incubation tank assembly is instantaneously simple and the detection accuracy is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of chemiluminescence immunoassay analyzers, and particularly relates to a incubation tank assembly and a detection method of a chemiluminescence immunoassay analyzer. Background Art

[0002] Currently, an annular incubation tank is often used in a chemiluminescence immunoassay analyzer to place reagent bottles and reaction cups. Among them, a reagent bottle contains a reagent amount for multiple persons for detecting multiple samples. During specific sample detection, the reagent bottle is opened, and then a reagent suction device is used to suck the reagent and mix it with the sample in the reaction cup for detection. Summary of the Invention

[0003] Although the annular incubation tank of the existing chemiluminescence immunoassay analyzer can realize sample detection, since the market positioning of the existing chemiluminescence immunoassay analyzer with an annular incubation tank is usually for large medical institutions, the annular incubation tank needs to be able to place multiple reaction cups and reagent bottles containing a large amount of reagent measurement to meet the requirements of multiple-person testing. However, it is found in practice that the reagent in the reagent bottle usually cannot be used up after opening, thus causing the problem of the opening validity period, that is, the remaining reagent after opening may cause a decrease in the detection accuracy of the sample when used again after a certain period of time. In addition, since the reaction cups and reagent bottles in the annular incubation tank exist independently, it is usually an important reason for the complex "calibration" process required during sample detection. "Calibration" requires professional operation and the operation process is complex, so that it is difficult to popularize the biochemical analyzer with an annular incubation tank in small medical institutions such as community hospitals.

[0004] In summary, the annular incubation tank of the existing chemiluminescence immunoassay analyzer has defects such as complex detection procedures of the chemiluminescence immunoassay analyzer and easy reduction of detection accuracy.

[0005] The present invention provides an incubation tank assembly of a chemiluminescence immunoassay analyzer to solve the above defects of the annular incubation tank of the existing chemiluminescence immunoassay analyzer. The incubation tank assembly of the chemiluminescence immunoassay analyzer includes:

[0006] An incubation tank with a linear channel inside; different operation positions communicating with the linear channel are provided along the direction of the linear channel on the upper surface of the incubation tank for chemiluminescence detection; a driving mechanism for driving a chip cartridge internally sealed with a disposable test reagent to pass is arranged on one side of the linear channel. Wherein, a part of the side wall of the chip cartridge opposite to the detection position can block light from entering, so that light cannot penetrate and irradiate the sample to be detected.

[0007] Preferably, the incubation tank is in a long strip structure.

[0008] Specifically, the operation positions include a puncture position, a sample addition position, a magnetic separation and cleaning position, and a detection position.

[0009] Specifically, a positioning sensor for sensing the position information of the chip cartridge is further provided on one side of the incubation tank; the positioning sensor is arranged near the chip cartridge inlet of the linear channel.

[0010] Specifically, the distances from the positioning sensor, the detection position, the sample addition position, the puncture position, and the magnetic separation and cleaning position to the chip cartridge inlet become farther in sequence.

[0011] Specifically, an identification position is arranged between the positioning sensor and the chip cartridge inlet; the identification position is communicated with the linear channel.

[0012] Specifically, a puncture fixing position is arranged beside the puncture position.

[0013] Specifically, a liquid suction fixing position is arranged beside the magnetic separation and cleaning position.

[0014] Specifically, the driving mechanism drives the chip cartridge to pass through by driving a clamping and limiting mechanism to move.

[0015] Specifically, the clamping and limiting mechanism includes: a slider connected to the driving mechanism, a clamping mechanism for clamping the chip cartridge on one side of the slider, and a limiting device for limiting the movement of the chip cartridge on one side of the driving mechanism.

[0016] The present invention also provides a detection method for a chemiluminescence immunoassay analyzer, including:

[0017] Providing a linear channel inside the incubation tank;

[0018] Providing different operation positions communicated with the linear channel along the direction of the linear channel on the upper surface of the incubation tank for chemiluminescence detection;

[0019] Providing a driving mechanism on one side of the linear channel;

[0020] Providing a chip cartridge; a disposable test reagent is sealed in the chip cartridge;

[0021] Driving the chip cartridge to pass through the linear channel by the driving mechanism.

[0022] The incubation tank assembly of the chemiluminescence immunoassay analyzer provided by the present invention is provided with a linear channel inside the incubation tank, different operation positions communicating with the linear channel are arranged along the direction of the linear channel on the upper surface of the incubation tank, a driving mechanism is arranged on one side of the linear channel, and then a chip cassette is provided. A disposable test reagent is sealed in the chip cassette, and then the chip cassette is driven by the driving mechanism to travel along the linear channel, thereby effectively avoiding the problem of the expiration date after opening the bottle and the operation of the "calibration" process, so that the detection process of the chemiluminescence immunoassay analyzer equipped with this incubation tank assembly is simple and immediate, and the detection accuracy is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 FIG. 6 is a schematic structural diagram of the incubation tank assembly of the chemiluminescence immunoassay analyzer provided in an embodiment;

[0024] Figure 2 FIG. 10 is a schematic structural diagram of the clamping mechanism provided in an embodiment;

[0025] Figure 3 FIG. 14 is a schematic flow chart of the detection method provided in an embodiment. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention.

[0027] In addition, in the description of the present invention, unless otherwise clearly defined and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can also be the internal communication of two elements. It can be a wireless connection or a wired connection. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0028] In addition, the technical features involved in different embodiments of the present invention described hereinafter can be combined with each other as long as they do not conflict with each other.

[0029] Next, the present invention proposes some preferred embodiments to teach those skilled in the art to implement.

[0030] Figure 1 The structural schematic diagram of the incubation tank assembly of a chemiluminescence immunoassay analyzer provided for an embodiment shows an incubation tank assembly of a chemiluminescence immunoassay analyzer. The incubation tank assembly of the chemiluminescence immunoassay analyzer includes:

[0031] See Figure 1 , an incubation tank 100 with a linear channel inside; along the direction of the linear channel on the upper surface of the incubation tank 100, different operation positions communicating with the linear channel are provided for chemiluminescence detection; on one side of the linear channel, a driving mechanism 103 for driving a chip cartridge containing a disposable test reagent to pass through is provided; the side wall of the chip cartridge can block light from entering the cartridge body.

[0032] Among them, the incubation tank 100 can be in a long strip structure. The operation positions can include a puncture position 108, a sample addition position 110, a magnetic separation and cleaning position 106, a detection position 111, etc. The setting order of the operation positions such as the puncture position 108, the sample addition position 110, the magnetic separation and cleaning position 106, and the detection position 111 on the incubation tank 100 can be set according to specific needs. Preferably, the distances of the detection position 111, the sample addition position 110, the puncture position 108, and the magnetic separation and cleaning position 106 from the chip cartridge inlet become farther in sequence.

[0033] Improved, a position sensor 112 for sensing the position information of the chip cartridge is further provided on one side of the incubation tank 100; the position sensor 112 is arranged near the chip cartridge inlet 116 of the linear channel. Preferably, the distances of the position sensor 112, the detection position 111, the sample addition position 110, the puncture position 108, and the magnetic separation and cleaning position 106 from the chip cartridge inlet 116 become farther in sequence.

[0034] Improved, an identification position 113 is provided between the position sensor 112 and the chip cartridge inlet 116; the identification position 113 communicates with the linear channel. Among them, the identification position 113 can identify the identification information (such as a two-dimensional code) of the chip cartridge, and can confirm the identity information of the chip cartridge, so as to avoid errors in the detection object. For example, the identification information of the chip cartridge can be scanned and obtained by a scanner 114 supported by a scanner bracket 115 above the incubation tank 100 for comparison with the background.

[0035] Improved, a puncture fixation position 109 is provided beside the puncture position 108, and a liquid suction fixation position 107 is provided beside the magnetic separation and cleaning position 106. Among them, the puncture fixation position 109 and the liquid suction fixation position 107 can play the roles of puncture fixation and magnetic absorption fixation respectively.

[0036] In this embodiment, a linear channel is arranged inside the incubation tank 100, different operation positions communicating with the linear channel are arranged along the direction of the linear channel on the upper surface of the incubation tank 100, a driving mechanism 103 is arranged on one side of the linear channel, a chip cassette is arranged, and a disposable test reagent is sealed in the chip cassette. Then, the chip cassette is driven by the driving mechanism 103 to travel along the linear channel, thereby effectively avoiding the problem of the opening validity period and the operation of the "calibration" process, so that the detection process of the chemiluminescence immunoassay analyzer equipped with the incubation tank 100 assembly is instantaneously simple and the detection accuracy is improved.

[0037] It should be noted that since the test reagent sealed in the chip cassette is a disposable reagent for single-person use, the test reagent is pre-sealed in the chip cassette through a sealing film in a single-use dosage. At the same time, the pre-sealed reagent has been calibrated through a special "calibration" process, and no complex "calibration" operation is required when using the test reagent, so that the detection process of the chemiluminescence immunoassay analyzer equipped with the incubation tank 100 assembly is instantaneously simple. Among them, because the test reagent is a sealed disposable reagent for single-person use, it can not only effectively avoid the problem of the opening validity period and prevent the test accuracy from being reduced due to the reuse of the retained reagent, but also avoid the need to set up an additional reagent suction device to suck the reagent into the reaction cup, thereby effectively reducing a reagent loading and unloading process.

[0038] In addition, when specifically using the incubation tank 100 assembly of the chemiluminescence immunoassay analyzer in this embodiment to detect and analyze a sample, the chip cassette is placed into the chip cassette inlet 116 of the linear channel, and then the chip cassette is driven by the driving mechanism 103 to travel in the linear channel, where it receives the puncture of the chip cassette sealing film at the puncture position 108, receives the sample addition to the punctured chip cassette at the sample addition position 110, and then receives the detection of the chemiluminescence value of the reactant after mixing the sample and the reagent at the detection position 111, so that an instant, efficient and simple sample test can be realized.

[0039] In addition, compared with the chemiluminescence immunoassay analyzer in the prior art that uses an annular incubation tank to place reagent bottles and reaction cups, and the reagent bottles are filled with reagent dosages for multiple people to detect multiple samples, the chemiluminescence immunoassay analyzer with the incubation tank 100 assembly provided in this embodiment has the advantages of instantaneously simple detection process and relatively high detection accuracy.

[0040] It should also be noted that the long-strip structure of the incubation tank 100 is conducive to setting the linear channel, and is conducive to arranging the puncture position 108, the sample addition position 110, the magnetic separation and cleaning position 106, and the detection position 111 along the linear channel, so that other mechanisms of the chemiluminescence immunoassay analyzer can be arranged along the long-strip structure of the incubation tank 100, thereby achieving the effect of concentrating the mechanism positions to reduce the overall volume of the chemiluminescence immunoassay analyzer.

[0041] It should also be noted that chemiluminescence detection is a specific detection method that is completely different from biochemical detection. In chemiluminescence detection, light is required not to penetrate the side wall of the chip cassette to irradiate the sample. Therefore, when detecting the chemiluminescence value of the reactant, it is necessary to avoid natural light from irradiating the reactant in the chip cassette. Therefore, the side wall of the chip cassette needs to be able to block light from entering the cassette body. Specifically, the chip cassette can be made of light-blocking materials.

[0042] It should also be noted that the incubation tank 100 has the functions of storing the reactants at a constant temperature and providing a suitable reaction temperature, and can specifically be composed of a heating device, a temperature sensor, a temperature adjustment device, etc.

[0043] Figure 2 Schematic diagram of the structure of the clamping mechanism provided for an embodiment, showing a clamping mechanism.

[0044] See Figure 1-2 , the driving mechanism 103 drives the chip cassette to pass by driving the movement of a clamping and limiting mechanism. The clamping and limiting mechanism includes: a slider 101 connected to the driving mechanism 103, a clamping mechanism 104 that clamps the chip cassette on one side of the slider 101, and a limiting device 102 that limits the movement of the chip cassette on one side of the driving mechanism 103.

[0045] Among them, the clamping mechanism 104 includes a clamping plate cover 1041, a middle partition 1042, a rotating hook pull-out pin 1043, a fixed hook 1044, a side partition 1045, and a bottom cover 1046. The middle partition 1042, the rotating hook pull-out pin 1043, the fixed hook 1044, and the side partition 1045 are located between the clamping plate cover 1041 and the bottom cover 1046. The rotating hook pull-out pin 1043 and the fixed hook 1044 are located between the middle partition 1042 and the bottom cover 1046, and can cooperate to clamp and release the chip cassette.

[0046] Figure 3 Schematic diagram of the process of the detection method provided for an embodiment, showing a detection method.

[0047] See Figure 3 , a detection method for a chemiluminescence immunoassay analyzer, includes:

[0048] S0. Set a linear channel inside the incubation tank;

[0049] S1. Set different operation positions communicating with the linear channel along the direction of the linear channel on the top of the incubation tank;

[0050] S2. Set a driving mechanism on one side of the linear channel;

[0051] S3. Set a chip cassette; the chip cassette is sealed with a disposable test reagent;

[0052] S4. Drive the chip cassette to pass through the linear channel by means of a driving mechanism.

[0053] Refer to Figure 1-3 In this embodiment, a linear channel is arranged inside the incubation tank 100, different operation positions communicating with the linear channel are arranged along the direction of the linear channel on the upper surface of the incubation tank 100, a driving mechanism 103 is arranged on one side of the linear channel, a chip cassette is arranged, a disposable test reagent is sealed in the chip cassette, and then the chip cassette is driven to pass through the linear channel by the driving mechanism 103, thereby effectively avoiding the generation of the problem of the opening validity period and the operation of the "calibration" process, so that the detection process of the chemiluminescence immunoassay analyzer equipped with the incubation tank 100 assembly is instantaneously simple and the detection accuracy is improved.

[0054] It should be noted that since the test reagent sealed in the chip cassette is a disposable reagent for single use per person, the test reagent is pre-sealed in the chip cassette by a sealing film with a single-use measurement. At the same time, the pre-sealed reagent has been calibrated through a special "calibration" process, and no complex "calibration" operation is required when using the test reagent, so that the detection process of the chemiluminescence immunoassay analyzer equipped with the incubation tank 100 assembly is instantaneously simple. Among them, because the test reagent is a sealed disposable reagent for single use per person, it can not only effectively avoid the problem of the opening validity period and prevent the test accuracy from being reduced due to the retention of the reagent and reuse, but also avoid the need to set up an additional reagent suction device to suck the reagent into the reaction cup, thereby effectively reducing a reagent loading and unloading process.

[0055] In addition, when specifically using the incubation tank 100 assembly of the chemiluminescence immunoassay analyzer in this embodiment to detect and analyze a sample, the chip cassette is placed into the chip cassette inlet 116 of the linear channel, and then the chip cassette is driven by the driving mechanism 103 to pass through the linear channel, receive the puncture of the sealing film of the chip cassette by the puncture position 108, receive the sample addition to the punctured chip cassette by the sample addition position 110, and then receive the detection of the chemiluminescence value of the reactant after mixing the sample and the reagent by the detection position 111, so as to realize instant, efficient and simple sample testing.

[0056] In addition, compared with the chemiluminescence immunoassay analyzer in the prior art that uses an annular incubation tank to place reagent bottles and reaction cups, and the reagent bottles are filled with reagent amounts for multiple persons to detect multiple samples, the chemiluminescence immunoassay analyzer with the incubation tank 100 assembly provided in this embodiment has the advantages of instantaneously simple detection process and relatively high detection accuracy.

[0057] It should also be noted that the incubation tank 100 with a long strip structure is conducive to setting up a straight channel, which is beneficial to setting up the puncture position 108, the sample addition position 110, the magnetic separation and cleaning position 106, and the detection position 111 along the straight channel, so that other mechanisms of the chemiluminescence immunoassay analyzer can be arranged along the incubation tank 100 with a long strip structure, thereby achieving the effect of concentrating the mechanism positions to reduce the overall volume of the chemiluminescence immunoassay analyzer.

[0058] It should also be noted that when detecting the chemiluminescence value of the reactant, it is necessary to avoid natural light irradiating the reactant in the chip cassette. Therefore, the side wall of the chip cassette needs to be able to block light from entering the cassette body. Specifically, the chip cassette can be made of light-impermeable materials.

[0059] It should also be noted that the incubation tank 100 has the functions of storing the reactant at a constant temperature and providing an appropriate reaction temperature, which can be specifically composed of a heating device, a temperature sensor, a temperature regulating device, etc.

[0060] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A incubation tank assembly of a chemiluminescence immunoassay analyzer, characterized in that, Comprising: An incubation tank with a straight channel inside; different operation positions communicating with the straight channel are provided along the direction of the straight channel on the upper surface of the incubation tank for chemiluminescence detection. The operation positions include a puncture position, a sample addition position, a magnetic separation and cleaning position, and a detection position; a driving mechanism capable of driving a chip cartridge containing a disposable test reagent to pass through is arranged on one side of the straight channel. The driving mechanism drives the chip cartridge to pass through by driving a clamping and limiting mechanism to move. The clamping and limiting mechanism includes: a slider connected to the driving mechanism, a clamping mechanism for clamping the chip cartridge on one side of the slider, and a limiting device for limiting the movement of the chip cartridge on one side of the driving mechanism.

2. The incubation tank assembly of the chemiluminescence immunoassay analyzer according to claim 1, characterized in that, An in-place sensor for sensing the position information of the chip cartridge is further arranged on one side of the incubation tank; the in-place sensor is arranged near the chip cartridge entrance of the straight channel.

3. The incubation tank assembly of the chemiluminescence immunoassay analyzer according to claim 2, characterized in that, The distances from the in-place sensor, the detection position, the sample addition position, the puncture position, and the magnetic separation and cleaning position to the chip cartridge entrance become farther in sequence.

4. The incubation tank assembly of the chemiluminescence immunoassay analyzer according to claim 2, characterized in that, An identification position is arranged between the in-place sensor and the chip cartridge entrance; the identification position communicates with the straight channel.

5. The incubation tank assembly of the chemiluminescence immunoassay analyzer according to claim 2, characterized in that, A puncture fixing position is arranged beside the puncture position.

6. The incubation tank assembly of the chemiluminescence immunoassay analyzer according to claim 2, characterized in that, A liquid suction fixing position is arranged beside the magnetic separation and cleaning position.

7. A detection method for the incubation tank assembly of the chemiluminescence immunoassay analyzer according to any one of claims 1-6, characterized in that, Comprising: A straight channel is arranged inside the incubation tank; Different operation positions communicating with the straight channel are arranged along the direction of the straight channel on the upper surface of the incubation tank for chemiluminescence detection; A driving mechanism is arranged on one side of the straight channel; A chip cartridge is provided; the chip cartridge contains a disposable test reagent; The chip cartridge is driven to pass through the straight channel by the driving mechanism.

Images